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Pipette Tip (pipette + tip)
Selected AbstractsSolid Contact Micropipette Ion Selective Electrode for Potentiometric SECMELECTROANALYSIS, Issue 10 2007Gergely Gyetvai Abstract New solid contact ammonium micropipette electrodes (ISE), well applicable in scanning electrochemical microscopy are reported. The solid contact was made of a PEDOT nanowire coated carbon fiber, lowered down close to the orifice, and dipped inside the cocktail being in the pipette tip. This configuration provided low electrical resistance and good potential stability. Submicron tip size, usual in case of micropipette ISE-s easily can be fabricated in this way. The applicability of the electrode in SECM has been proved in SG/TC mode imaging urease enzyme active spots in urea solutions. [source] Growth of large protein crystals by a large-scale hanging-drop methodJOURNAL OF APPLIED CRYSTALLOGRAPHY, Issue 4 2010Keisuke Kakinouchi A method for growing large protein crystals is described. In this method, a cut pipette tip is used to hang large-scale droplets (maximum volume 200,µl) consisting of protein and precipitating agents. A crystal grows at the vapor,liquid interface; thereafter the grown crystal can be retrieved by droplet,droplet contact both for repeated macroseeding and for mounting crystals in a capillary. Crystallization experiments with peroxiredoxin of Aeropyrum pernix K1 (thioredoxin peroxidase, ApTPx) and hen egg white lysozyme demonstrated that this large-scale hanging-drop method could produce a large-volume crystal very effectively. A neutron diffraction experiment confirmed that an ApTPx crystal (6.2,mm3) obtained by this method diffracted to beyond 3.5,Ĺ resolution. [source] A convenient purification and preconcentration of peptides with ,-cyano-4-hydroxycinnamic acid matrix crystals in a pipette tip for matrix-assisted laser desorption/ionization mass spectrometry,JOURNAL OF MASS SPECTROMETRY (INCORP BIOLOGICAL MASS SPECTROMETRY), Issue 1 2010Helena, ehulková Abstract Peptide samples derived from enzymatic in-gel digestion of proteins resolved by gel electrophoresis often contain high amount of salts originating from reaction and separation buffers. Different methods are used for desalting prior to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), e.g. reversed-phase pipette tip purification, on-target washing, adding co-matrices, etc. As a suitable matrix for MALDI MS of peptides, ,-cyano-4-hydroxycinnamic acid (CHCA) is frequently used. Crystalline CHCA shows the ability to bind peptides on its surface and because it is almost insoluble in acidic water solutions, the on-target washing of peptide samples can significantly improve MALDI MS signals. Although the common on-target washing represents a simple, cheap and fast procedure, only a small portion of the available peptide solution is efficiently used for the subsequent MS analysis. The present approach is a combination of the on-target washing principle carried out in a narrow-end pipette tip (e.g. GELoader tip) and preconcentration of peptides from acidified solution by passing it through small CHCA crystals captured inside the tip on a glass microfiber frit. The results of MALDI MS analysis using CHCA-tip peptide preconcentration are comparable with the use of homemade POROS R2 pipette tip microcolumns. Advantages and limitations of this approach are discussed. Copyright © 2009 John Wiley & Sons, Ltd. [source] DigesTip: A new device for a rapid and efficient in-solution protein digestionPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 11 2008Roberto Marangoni Dr. Abstract DigesTip is a new device for in-solution protein digestion, based on a patent pending technology, able to immobilize enzymes (trypsin, in this case) on a solid surface, keeping their activity preserved. DigesTip is a standard pipette tip, usable both by human and by robots. Its main performances are: very short digestion time (1,min) and usability with low protein sample concentrations (5,,g/mL). DigesTip obtains a clear signal in MS measurements and its usage rules out several preparative steps. [source] A convenient and versatile hydroponic cultivation system for Arabidopsis thalianaPHYSIOLOGIA PLANTARUM, Issue 3 2004Hanna Norén A versatile two-step cultivation procedure for Arabidopsis thaliana is described for the production of large quantities of leaf material suitable for biochemical and biophysical analysis. The first step comprises a miniature greenhouse made out of a plastic pipette box to grow the seedlings to the six-leaf stage. For continued growth, the seedlings are transferred to hydroponic cultivation using an opaque container covered by a styrofoam lid. Transfer of the small seedlings to hydroponic culture is facilitated by growth in separate pipette tips, which protects vulnerable roots from damage. The hydroponic cultivation system is easy to scale-up and produces large amounts of relatively large leaves and roots. This hydroponic system produces enough plant material to make Arabidopsis a feasible model for biochemical and biophysical experiments, which can be combined with the available genetic information to address various aspects of plant functional genomics. [source] Electrospray ionization using disposable plastic pipette tipsRAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 19 2001Sergei Aksyonov No abstract is available for this article. [source] | ||||||||||