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Physical Parameters (physical + parameter)
Selected AbstractsEngine-Driven Preparation Of Curved Root Canals: Measuring Cyclic Fatigue And Other Physical Parameters,AUSTRALIAN ENDODONTIC JOURNAL, Issue 1 2002Ove A. Peters Dr med dent An increasing number of engine-driven rotary systems are marketed to shape root canals. Although these systems may improve the quality of canal preparations, the risk for instrument fracture is also increased. Unfortunately, the stresses generated in rotary instruments when shaping curved root canals have not been adequately studied. Consequently, the aim of an ongoing project was to develop a measurement platform that could more accurately detail physical parameters generated in a simulated clinical situation. Such a platform was constructed by fitting a torque-measuring device between the rotating endodontic instrument and the motor driving it. Apically directed force and instrument insertion depth were also recorded. Additional devices were constructed to assess cyclic fatigue and static fracture loads. The current pilot study evaluated GT rotary instruments during the shaping of curved canals in plastic blocks as well as "ISO 3630,1 torque to fracture" and number of rotations required for fatigue fracture. Results indicated that torques in excess of 40Nmm were generated by rotary GT-Files, a significantly higher figure than static fracture loads (less than 13Nmm for the size 20. 12 GT-File). Furthermore, the number of rotations needed to shape simulated canals with a 5 mm radius of curvature in plastic blocks was 10 times lower than the number of rotations needed to fracture instruments in a "cyclic fatigue test". Apical forces were always greater than IN, and in some specimens, scores of 8N or more were recorded. Further studies are required using extracted natural teeth, with their wide anatomical variation, in order to reduce the incidence of fracture of rotary instruments. In this way, the clinical potential of engine-driven rotary instruments to safely prepare curved canals can be fully appreciated. [source] EFFECTS OF KERNEL PROPERTIES AND POPPING METHODS ON POPCORN QUALITY OF DIFFERENT CORN CULTIVARSJOURNAL OF FOOD PROCESS ENGINEERING, Issue 4 2009NILGÜN ERTA ABSTRACT Three popcorn genotypes, Koç Cin (composite), Nermin Cin and Ant Cin-98 (hybrid) were investigated for the effects of kernel size (4 < D < 5 and 5 < D < 6), popping methods (conventional and microwave) and moisture content (10, 12 and 14%) on popcorn quality. The relationships between physical kernel properties and popping characteristics were also researched. The Nermin Cin popcorn cultivar had the highest expansion volume, flake size and the lowest percentage of unpopped kernel values among genotypes. 5 > D > 6 mm kernel fraction and the conventional method produced higher popcorn quality in all popcorn cultivars. The optimum moisture levels for the highest expansion volume changed between 12 and 14% for different cultivars. The correlation coefficient between kernel properties (width, sphericity, L, a and b) and expansion volume were found significant (P < 0.01) in microwave popping method. Color a had high direct effects on expansion volume in conventional and microwave popping methods according to path coefficient analysis. L values of popcorn cultivars negatively correlated with expansion volume in both popping methods. PRACTICAL APPLICATIONS Physical parameters like width, sphericity and color values were found important in predicting popping quality. Color values should be used to predict expansion volume instead of classic physical properties for ease ofmeasurement. Conventional popping method, 5 > D > 6 kernel size and 12,14% moisture content parameters should be used for optimum popcorn quality. [source] EFFECT OF THAWING METHODS ON TEXTURAL QUALITY OF SOUS-VIDE STEWED BEEF ASSESSED BY SENSORY AND INSTRUMENTAL ANALYSESJOURNAL OF FOOD QUALITY, Issue 5 2001N.J.N. YAU ABSTRACT This study compared four different thawing methods using sensory test with magnitude estimation, preference test with hedonic scale and physical analyses. The four methods were tap-water, refrigeration, microwave reheating, and boiling-water reheating. For physical analyses, there were differences (p,0.001) among treatments in drip loss and expressible juice. For drip loss, the microwave reheated samples were the highest and the refrigeration and tap-water treated samples were the lowest. For expressible juice, tap-water and refrigerated samples were higher (p,0.01) than the microwave and boiling-water reheated samples. Microwave and boiling-water reheated samples had higher hardness and cheviness and lower juiciness and fiber-looseness. The refrigerated and tap-water thawed samples had reverse sensory properties. Naive panelists preferred the refrigerated and tap-water treated samples to the microwave and boiling-water reheated samples for juiciness, tenderness, and overall preference. Physical parameters from textural profile analysis were not different among the thawing methods. Expressible juice was the best indicator for sensory hardness, juiciness, fiber-looseness and chewiness among the physical parameters. Usually a quadratic regression model fit best when expressible juice was used to predict sensory textural properties. [source] Head,tail Galaxies: beacons of high-density regions in clustersMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 3 2009Minnie Y. Mao ABSTRACT Using radio data at 1.4 GHz from the Australia Telescope Compact Array (ATCA), we identify five head,tail (HT) galaxies in the central region of the Horologium,Reticulum Supercluster (HRS). Physical parameters of the HT galaxies were determined along with substructure in the HRS to probe the relationship between environment and radio properties. Using a density enhancement technique applied to 582 spectroscopic measurements in the 2°× 2° region about A3125/A3128, we find all five HT galaxies reside in regions of extremely high density (>100 galaxies Mpc,3). In fact, the environments surrounding HT galaxies are statistically denser than those environments surrounding non-HT galaxies and among the densest environments in a cluster. Additionally, the HT galaxies are found in regions of enhanced X-ray emission and we show that the enhanced density continues out to substructure groups of 10 members. We propose that it is the high densities that allow ram pressure to bend the HT galaxies as opposed to previously proposed mechanisms relying on exceptionally high peculiar velocities. [source] Physical parameters of the O6.5V+B1V eclipsing binary system LS 1135MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 4 2006E. Fernández Lajús ABSTRACT The ,All Sky Automated Survey' (ASAS) photometric observations of LS 1135, an O-type single-lined binary (SB1) system with an orbital period of 2.7 d, show that the system is also eclipsing performing a numerical model of this binary based on the Wilson,Devinney method. We obtained an orbital inclination . With this value of the inclination, we deduced masses M1, 30 ± 1 M, and M2, 9 ± 1 M,, and radii R1, 12 ± 1 R, and R2, 5 ± 1 R, for primary and secondary components, respectively. Both the components are well inside their respective Roche lobes. Fixing the Teff of the primary to the value corresponding to its spectral type (O6.5V), the Teff obtained for the secondary component corresponds approximately to a spectral type of B1V. The mass ratio M2/M1, 0.3 is among the lowest known values for spectroscopic binaries with O-type components. [source] Bond rolling resistance and its effect on yielding of bonded granulates by DEM analysesINTERNATIONAL JOURNAL FOR NUMERICAL AND ANALYTICAL METHODS IN GEOMECHANICS, Issue 8 2006M. J. Jiang Abstract A discrete element modelling of bonded granulates and investigation on the bond effect on their behaviour are very important to geomechanics. This paper presents a two-dimensional (2-D) discrete element theory for bonded granulates with bond rolling resistance and provides a numerical investigation into the effect of bond rolling resistance on the yielding of bonded granulates. The model consists of mechanical contact models and equations governing the motion of bonded particles. The key point of the theory is that the assumption in the original bond contact model previously proposed by the authors (55th CSCE-ASCE Conference, Hamilton, Ont., Canada, 2002; 313,320; J. Eng. Mech. (ASCE) 2005; 131(11):1209,1213) that bonded particles are in contact at discrete points, is here replaced by a more reliable assumption that bonded particles are in contact over a width. By making the idealization that the bond contact width is continuously distributed with the normal/tangential basic elements (BE) (each BE is composed of spring, dashpot, bond, slider or divider), we establish a bond rolling contact model together with bond normal/tangential contact models, and also relate the governing equations to local equilibrium. Only one physical parameter , needs to be introduced in the theory in comparison to the original bond discrete element model. The model has been implemented into a 2-D distinct element method code, NS2D. Using the NS2D, a total of 86 1-D, constant stress ratio, and biaxial compressions tests have been carried out on the bonded granular samples of different densities, bonding strengths and rolling resistances. The numerical results show that: (i) the new theory predicts a larger internal friction angle, a larger yielding stress, more brittle behaviour and larger final broken contact ratio than the original bond model; (ii) the yielding stress increases nonlinearly with the increasing value of ,, and (iii) the first-yield curve (initiation of bond breakage), which define a zone of none bond breakage and which shape and size are affected by the material density, is amplified by the bond rolling resistance in analogous to that predicted by the original bond model. Copyright © 2006 John Wiley & Sons, Ltd. [source] Synchronization patterns in spaghetti-like nanoclustersINTERNATIONAL JOURNAL OF QUANTUM CHEMISTRY, Issue 15 2008Acep Purqon Abstract Spaghetti-like nanoclusters concern disordered shapes and irregular shape fluctuations in few correlated biological lipids. We evaluate the shape fluctuations by introducing Symmetry- S as a physical parameter for measuring symmetry degrees and detecting shape transitions. From numerical simulation of few correlated lipids of POPC and POPE at 300 K and 340 K by using molecular dynamics, we investigate the symmetry dynamics for each individual cluster by analyzing both spatiotemporal and frequency. From spatiotemporal analysis, we find several jump motions in S -dynamics and non-Gaussian distributions in S -distribution. Interestingly, the jump motions likely contribute on the existence of transitions in the non-Gaussian distributions. Additionally, even number of lipids show more symmetric than the odd number of lipids and the symmetry distributions shift at higher temperature, while, from three dimension of actual position of symmetry dynamics, they are not easy to configure high symmetry as well as showing certain patterns. From power spectra density analysis, each individual cluster shows nearly random fluctuation. Besides individual clusters, we also investigate mutual clusters. Surprisingly, although individual clusters show fluctuations randomly, mutual clusters show certain direction correlations. Moreover, they show certain patterns in delayed time analysis such as mutual fluctuations periodically occur for same number of lipids. It indicates that an existence of synchronization patterns occur in shape fluctuations of spaghetti-like nanoclusters. © 2008 Wiley Periodicals, Inc. Int J Quantum Chem, 2008 [source] The effects of osmotic stress on the structure and function of the cell nucleusJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 3 2010John D. Finan Abstract Osmotic stress is a potent regulator of the normal function of cells that are exposed to osmotically active environments under physiologic or pathologic conditions. The ability of cells to alter gene expression and metabolic activity in response to changes in the osmotic environment provides an additional regulatory mechanism for a diverse array of tissues and organs in the human body. In addition to the activation of various osmotically- or volume-activated ion channels, osmotic stress may also act on the genome via a direct biophysical pathway. Changes in extracellular osmolality alter cell volume, and therefore, the concentration of intracellular macromolecules. In turn, intracellular macromolecule concentration is a key physical parameter affecting the spatial organization and pressurization of the nucleus. Hyper-osmotic stress shrinks the nucleus and causes it to assume a convoluted shape, whereas hypo-osmotic stress swells the nucleus to a size that is limited by stretch of the nuclear lamina and induces a smooth, round shape of the nucleus. These behaviors are consistent with a model of the nucleus as a charged core/shell structure pressurized by uneven partition of macromolecules between the nucleoplasm and the cytoplasm. These osmotically-induced alterations in the internal structure and arrangement of chromatin, as well as potential changes in the nuclear membrane and pores are hypothesized to influence gene transcription and/or nucleocytoplasmic transport. A further understanding of the biophysical and biochemical mechanisms involved in these processes would have important ramifications for a range of fields including differentiation, migration, mechanotransduction, DNA repair, and tumorigenesis. J. Cell. Biochem. 109: 460,467, 2010. © 2009 Wiley-Liss, Inc. [source] A new space and time sensor fusion method for mobile robot navigationJOURNAL OF FIELD ROBOTICS (FORMERLY JOURNAL OF ROBOTIC SYSTEMS), Issue 7 2004TaeSeok Jin To fully utilize the information from the sensors of mobile robot, this paper proposes a new sensor-fusion technique where the sample data set obtained at a previous instant is properly transformed and fused with the current data sets to produce a reliable estimate for navigation control. Exploration of an unknown environment is an important task for the new generation of mobile service robots. The mobile robots may navigate by means of a number of monitoring systems such as the sonar-sensing system or the visual-sensing system. Notice that in the conventional fusion schemes, the measurement is dependent on the current data sets only. Therefore, more sensors are required to measure a given physical parameter or to improve the reliability of the measurement. However, in this approach, instead of adding more sensors to the system, the temporal sequences of the data sets are stored and utilized for the purpose. The basic principle is illustrated by examples and the effectiveness is proved through simulations and experiments. The newly proposed STSF (space and time sensor fusion) scheme is applied to the navigation of a mobile robot in an environment using landmarks, and the experimental results demonstrate the effective performance of the system. © 2004 Wiley Periodicals, Inc. [source] The long-time behaviour of the thermoconvective flow in a porous mediumMATHEMATICAL METHODS IN THE APPLIED SCIENCES, Issue 8 2004M. A. Efendiev Abstract For the Boussinesq approximation of the equations of coupled heat and fluid flow in a porous medium we show that the corresponding system of partial differential equations possesses a global attractor. We give lower and upper bounds of the Hausdorff dimension of the attractor depending on a physical parameter of the system, namely the Rayleigh number of the flow. Numerical experiments confirm the theoretical findings and raise new questions on the structure of the solutions of the system. Copyright © 2004 John Wiley & Sons, Ltd. [source] Particle energies and filling fractions of radio bubbles in cluster coresMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 3 2004R. J. H. Dunn ABSTRACT Using Chandra images of cluster cores with clear radio bubbles, we have determined k, which is the ratio of the total particle energy to that of the electrons radiating between 10 MHz and 10 GHz. Radiative and dynamical constraints on the bubbles indicate that the ratio of the energy factor, k, to the volume filling factor, f, lies within the range 1 ,k/f, 1000. Assuming pressure equilibrium between the radio-emitting plasma and the surrounding X-ray gas, none of the lobes has equipartition between relativistic particles and magnetic field. There is no evidence for any dependence of the upper limit of the k/f ratio on any physical parameter of the cluster or the radio source. The distribution of the upper limit on k/f appears to be bimodal, the value being ,3 for some clusters and ,300 for the others. We show that this may be due to the composition of the jet which forms the bubbles, the variation in the volume filling fraction or variation in the amount of reacceleration occurring in the bubble. [source] Influence of dispersion length on leaching calculated with PEARL, PELMO and PRZM for FOCUS groundwater scenariosPEST MANAGEMENT SCIENCE (FORMERLY: PESTICIDE SCIENCE), Issue 10 2004Jos JTI Boesten Abstract Harmonisation of the assessment of pesticide leaching to groundwater for EU registration is desirable to minimise confusion in the decision-making process at EU level. Recently, the FOCUS groundwater scenarios have been developed for three chromatographic models (PEARL, PELMO and PRZM) to increase this harmonisation. This study investigates the role of dispersion parameterisation in explaining the cause of the differences in pesticide leaching calculated by these models. PEARL describes dispersion via a physical parameter, ie the dispersion length. PELMO and PRZM simulate dispersion via a numerical procedure which generates an effective dispersion length equal to 0.5 times the thickness of the numerical compartments. The hypothesis was tested that the difference in the dispersion length input parameter (ie 5 cm for PEARL and about 2.5 cm for PELMO and PRZM) is a major cause of the difference in calculated leaching. It was tested whether results of PEARL calculations with a dispersion length of 2.5 cm corresponded much better to results of PELMO or PRZM than results of PEARL calculations with a dispersion length of 5 cm. This was done by calculations for one substance and all nine FOCUS scenarios and by calculations for a range of substances and two FOCUS scenarios (Châteaudun and Sevilla). All calculations were for winter wheat and an application at 1 day before emergence. Both tests showed that reduction of the dispersion length from 5 to 2.5 cm in PEARL led to a much better correspondence between PEARL and either PELMO or PRZM. Hence the hypothesis was supported. It is likely that harmonisation of the dispersion length in the FOCUS groundwater scenarios would reduce the differences in calculated leaching between PEARL and PELMO or PRZM considerably for part of these scenarios. Published in 2004 for SCI by John Wiley & Sons, Ltd. [source] Molecular thermodynamic model of the glass transition temperature: dependence on molecular weightPOLYMERS FOR ADVANCED TECHNOLOGIES, Issue 8 2008Yong Woo Kim Abstract A new molecular thermodynamic model to predict the dependency of the glass transition temperature (Tg) of polymer on its molecular weight was developed based on the configurational entropy model and the Flory,Huggins theory. In this model, the disorientation entropy of the polymer (Sdis) has been taken into account. Quantitative descriptions according to the proposed model are consistent with experimental Tg data of several polymers against the number of chain segment (r). At the same Tg., (Tg of polymer at a infinite r value), the degree of polymer disorientation is strongly correlated with the slope of straight line at lower r regions in the Tg versus r plot, which is quantitatively identified by physical parameter (,dis) in this model. Copyright © 2008 John Wiley & Sons, Ltd. [source] Progress in rational methods of cryoprotection in macromolecular crystallographyACTA CRYSTALLOGRAPHICA SECTION D, Issue 4 2010Thomas Alcorn Cryogenic cooling of macromolecular crystals is commonly used for X-ray data collection both to reduce crystal damage from radiation and to gather functional information by cryogenically trapping intermediates. However, the cooling process can damage the crystals. Limiting cooling-induced crystal damage often requires cryoprotection strategies, which can involve substantial screening of solution conditions and cooling protocols. Here, recent developments directed towards rational methods for cryoprotection are described. Crystal damage is described in the context of the temperature response of the crystal as a thermodynamic system. As such, the internal and external parts of the crystal typically have different cryoprotection requirements. A key physical parameter, the thermal contraction, of 26 different cryoprotective solutions was measured between 294 and 72,K. The range of contractions was 2,13%, with the more polar cryosolutions contracting less. The potential uses of these results in the development of cryocooling conditions, as well as recent developments in determining minimum cryosolution soaking times, are discussed. [source] Crystal quality and differential crystal-growth behaviour of three proteins crystallized in gel at high hydrostatic pressureACTA CRYSTALLOGRAPHICA SECTION D, Issue 6 2005A. Kadri Pressure is a non-invasive physical parameter that can be used to control and influence protein crystallization. It is also found that protein crystals of superior quality can be produced in gel. Here, a novel crystallization strategy combining hydrostatic pressure and agarose gel is described. Comparative experiments were conducted on hen and turkey egg-white lysozymes and the plant protein thaumatin. Crystals could be produced under up to 75,100,MPa (lysozymes) and 250,MPa (thaumatin). Several pressure-dependent parameters were determined, which included solubility and supersaturation of the proteins, number, size and morphology of the crystals, and the crystallization volume. Exploration of three-dimensional phase diagrams in which pH and pressure varied identified growth conditions where crystals had largest size and best morphology. As a general trend, nucleation and crystal-growth kinetics are altered and nucleation is always enhanced under pressure. Further, solubility of the lysozymes increases with pressure while that of thaumatin decreases. Likewise, changes in crystallization volumes at high and atmospheric pressure are opposite, being positive for the lysozymes and negative for thaumatin. Crystal quality was estimated by analysis of Bragg reflection profiles and X-ray topographs. While the quality of lysozyme crystals deteriorates as pressure increases, that of thaumatin crystals improves, with more homogeneous crystal morphology suggesting that pressure selectively dissociates ill-formed nuclei. Analysis of the thaumatin structure reveals a less hydrated solvent shell around the protein when pressure increases, with ,20% less ordered water molecules in crystals grown at 150,MPa when compared with those grown at atmospheric pressure (0.1,MPa). Noticeably, the altered water distribution is seen in depressurized crystals, indicating that pressure triggers a stable structural alteration on the protein surface while its polypeptide backbone remains essentially unaltered. [source] Effect of short-range forces on the length distribution of fibrous cytoskeletal proteinsBIOPOLYMERS, Issue 9 2008David Popp Abstract The length distribution of cytoskeletal filaments is an important physical parameter, which can modulate physiological cell functions. In both eukaryotic and prokaryotic cells various biological cytoskeletal polymers form supramolecular structures due to short-range forces induced mainly by molecular crowding or cross linking proteins, but their in vivo length distribution remains difficult to measure. In general, based on experimental evidence and mathematical modeling of actin filaments in aqueous solutions, the steady state length distribution of fibrous proteins is believed to be exponential. We performed in vitro TIRF- and electron-microscopy to demonstrate that in the presence of short-range forces, which are an integral part of any living cell, the steady state length distributions of the eukaryotic cytoskeletal biopolymer actin, its prokaryotic homolog ParM and microtubule homolog FtsZ deviate from the classical exponential and are either double-exponential or Gaussian, as recent theoretical modeling predicts. Double exponential or Gaussian distributions opposed to exponential can change for example the visco-elastic properties of actin networks within the cell, influence cell motility by decreasing the amount of free ends at the leading edge of the cell or effect the assembly of FtsZ into the bacterial Z-ring thus modulating membrane constriction. © 2008 Wiley Periodicals, Inc. Biopolymers 89: 711,721, 2008. This article was originally published online as an accepted preprint. The "Published Online" date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com [source] Real-time cartoon animation of smokeCOMPUTER ANIMATION AND VIRTUAL WORLDS (PREV: JNL OF VISUALISATION & COMPUTER ANIMATION), Issue 3-4 2005Haitao He Abstract In this paper, we present a practical framework to generate cartoon style animations of smoke, which consists of two components: a smoke simulator and a rendering system. In the simulation stage, the smoke is modelled as a set of smoothed particles and the physical parameters such as velocity and force are defined on particles directly. The smoke is rendered in flicker-free cartoon style with two-tone shading and silhouettes. Both the simulation and rendering are intuitive and easy to implement. In the most moderate scale scene, an impressive cartoon animation is generated with about a thousand particles at real-time frame rate. Copyright © 2005 John Wiley & Sons, Ltd. [source] Connecting patient needs with treatment managementACTA PSYCHIATRICA SCANDINAVICA, Issue 2009R. Kerwin Objective:, To propose ideas for the development of a core strategy for monitoring patients with schizophrenia to ensure physical health and optimal treatment provision. Method:, A panel of European experts in the field of schizophrenia met in Bordeaux in June 2006 to discuss, ,Patient management optimisation through improved treatment monitoring.' Results:, Key consensus from the discussion deemed that weight gain, oral health and ECG parameters were core baseline parameters to be monitored in all patients with schizophrenia. Further, an identification of a patient's own barriers to treatment alongside local health service strategies might comprise elements of an individualised management strategy which would contribute to optimisation of treatment. Any monitoring strategy should be kept simple to encourage physician compliance. Conclusion:, A practical solution to the difficulties of providing holistic patient care would be to suggest a limited set of physical parameters to be monitored by physicians on a regular basis. [source] Parameter identification of framed structures using an improved finite element model-updating method,Part I: formulation and verificationEARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 5 2007Eunjong Yu Abstract In this study, we formulate an improved finite element model-updating method to address the numerical difficulties associated with ill conditioning and rank deficiency. These complications are frequently encountered model-updating problems, and occur when the identification of a larger number of physical parameters is attempted than that warranted by the information content of the experimental data. Based on the standard bounded variables least-squares (BVLS) method, which incorporates the usual upper/lower-bound constraints, the proposed method (henceforth referred to as BVLSrc) is equipped with novel sensitivity-based relative constraints. The relative constraints are automatically constructed using the correlation coefficients between the sensitivity vectors of updating parameters. The veracity and effectiveness of BVLSrc is investigated through the simulated, yet realistic, forced-vibration testing of a simple framed structure using its frequency response function as input data. By comparing the results of BVLSrc with those obtained via (the competing) pure BVLS and regularization methods, we show that BVLSrc and regularization methods yield approximate solutions with similar and sufficiently high accuracy, while pure BVLS method yields physically inadmissible solutions. We further demonstrate that BVLSrc is computationally more efficient, because, unlike regularization methods, it does not require the laborious a priori calculations to determine an optimal penalty parameter, and its results are far less sensitive to the initial estimates of the updating parameters. Copyright © 2006 John Wiley & Sons, Ltd. [source] A new green fluorescent protein-based bacterial biosensor for analysing phenanthrene fluxesENVIRONMENTAL MICROBIOLOGY, Issue 4 2006Robin Tecon Summary The polycyclic aromatic hydrocarbon (PAH)-degrading strain Burkholderia sp. RP007 served as host strain for the design of a bacterial biosensor for the detection of phenanthrene. RP007 was transformed with a reporter plasmid containing a transcriptional fusion between the phnS putative promoter/operator region and the gene encoding the enhanced green fluorescent protein (GFP). The resulting bacterial biosensor ,Burkholderia sp. strain RP037 , produced significant amounts of GFP after batch incubation in the presence of phenanthrene crystals. Co-incubation with acetate did not disturb the phenanthrene-specific response but resulted in a homogenously responding population of cells. Active metabolism was required for induction with phenanthrene. The magnitude of GFP induction was influenced by physical parameters affecting the phenanthrene flux to the cells, such as the contact surface area between solid phenanthrene and the aqueous phase, addition of surfactant, and slow phenanthrene release from Model Polymer Release System beads or from a water-immiscible oil. These results strongly suggest that the bacterial biosensor can sense different phenanthrene fluxes while maintaining phenanthrene metabolism, thus acting as a genuine sensor for phenanthrene bioavailability. A relationship between GFP production and phenanthrene mass transfer is proposed. [source] Extraction, purification and characterization of wax from flax (Linum usitatissimum) strawEUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, Issue 7 2009Yasantha Athukorala Abstract The chemical composition and selected physical parameters of wax extracted from flax straw with supercritical CO2 (SC-CO2) and hexane have been determined. From the GC/MS results, clear variations in composition and component distributions were observed between SC-CO2 - and hexane-extracted samples. The major components of the SC-CO2 and hexane extracts from three flax cultivars were: fatty acids (36,49%), fatty alcohols (20,26%), aldehydes (10,14%), wax esters (5,12%), sterols (7,9%) and alkanes (4,5%). Purification of SC-CO2 -extracted wax with silica gel chromatography yielded 0.4,0.5% (dry matter) and was composed primarily of wax esters (C44, C46 and C48) and alkanes (C27, C29 and C31). UV-Vis scans of the purified wax samples exhibited two main peaks indicating the presence of conjugated dienes and carotenoids or related compounds. Fourier transform infrared results showed prominent peaks at 2918 (-C-H), 2849 (-C-H), 1745 (-C=O), 1462 (-C-H), 1169 (-C-O) and 719,cm,1 (-(CH2)n -), with NorLin wax showing a slightly deviating pattern compared to the other samples. Thermal analysis by differential scanning calorimetry revealed a mean melting point of 55,56,°C and oxidation temperatures of 146,153,°C for purified wax from flax straw processed using different procedures. [source] Peptidoglycan structure and architectureFEMS MICROBIOLOGY REVIEWS, Issue 2 2008Waldemar Vollmer Abstract The peptidoglycan (murein) sacculus is a unique and essential structural element in the cell wall of most bacteria. Made of glycan strands cross-linked by short peptides, the sacculus forms a closed, bag-shaped structure surrounding the cytoplasmic membrane. There is a high diversity in the composition and sequence of the peptides in the peptidoglycan from different species. Furthermore, in several species examined, the fine structure of the peptidoglycan significantly varies with the growth conditions. Limited number of biophysical data on the thickness, elasticity and porosity of peptidoglycan are available. The different models for the architecture of peptidoglycan are discussed with respect to structural and physical parameters. [source] Detecting the impact of oceano-climatic changes on marine ecosystems using a multivariate index: The case of the Bay of Biscay (North Atlantic-European Ocean)GLOBAL CHANGE BIOLOGY, Issue 1 2008GEORGES HEMERY Abstract Large-scale univariate climate indices (such as NAO) are thought to outperform local weather variables in the explanation of trends in animal numbers but are not always suitable to describe regional scale patterns. We advocate the use of a Multivariate Oceanic and Climatic index (MOCI), derived from ,synthetic' and independent variables from a linear combination of the total initial variables objectively obtained from Principal Component Analysis. We test the efficacy of the index using long-term data from marine animal populations. The study area is the southern half of the Bay of Biscay (43°,47°N; western Europe). Between 1974 and 2000 we monitored cetaceans and seabirds along 131000 standardized line transects from ships. Fish abundance was derived from commercial fishery landings. We used 44 initial variables describing the oceanic and atmospheric conditions and characterizing the four annual seasons in the Bay of Biscay. The first principal component of our MOCI is called the South Biscay Climate (SBC) index. The winter NAO index was correlated to this SBC index. Inter-annual fluctuations for most seabird, cetacean and fish populations were significant. Boreal species (e.g. gadiformes fish species, European storm petrel and Razorbill ,) with affinities to cold temperate waters declined significantly over time while two (Puffin and Killer Whale) totally disappeared from the area during the study period. Meridional species with affinities to hotter waters increased in population size. Those medium-term demographic trends may reveal a regime shift for this part of the Atlantic Ocean. Most of the specific observed trends were highly correlated to the SBC index and not to the NAO. Between 40% and 60% of temporal variations in species abundance were explained by the multivariate SBC index suggesting that the whole marine ecosystem is strongly affected by a limited number of physical parameters revealed by the multivariate SBC index. Aside the statistical error of the field measurements, the remaining variation unexplained by the physical characteristics of the environment correspond to the impact of anthropogenic activities such overfishing and oil-spills. [source] Arsenic in Glacial Drift Aquifers and the Implication for Drinking Water,Lower Illinois River BasinGROUND WATER, Issue 3 2001Kelly L. Warner The lower Illinois River Basin (LIRB) covers 47,000 km2 of central and western Illinois. In the LIRB, 90% of the ground water supplies are from the deep and shallow glacial drift aquifers. The deep glacial drift aquifer (DGDA) is below 152 m altitude, a sand and gravel deposit that fills the Mahomet Buried Bedrock Valley, and overlain by more than 30.5 m of clayey till. The LIRB is part of the USGS National Water Quality Assessment program, which has an objective to describe the status and trends of surface and ground water quality. In the DGDA, 55% of the wells used for public drinking-water supply and 43% of the wells used for domestic drinking water supply have arsenic concentrations above 10 ,g/L (a new U.S. EPA drinking water standard). Arsenic concentrations greater than 25 ,g/L in ground water are mostly in the form of arsenite (AsIII). The proportion of arsenate (AsV) to arsenite does not change along the flowpath of the DGDA. Because of the limited number of arsenic species analyses, no clear relations between species and other trace elements, major ions, or physical parameters could be established. Arsenic and barium concentrations increase from east to west in the DGDA and are positively correlated. Chloride and arsenic are positively correlated and provide evidence that arsenic may be derived locally from underlying bedrock. Solid phase geochemical analysis of the till, sand and gravel, and bedrock show the highest presence of arsenic in the underlying organic-rich carbonate bedrock. The black shale or coal within the organic-rich carbonate bedrock is a potential source of arsenic. Most high arsenic concentrations found in the DGDA are west and downgradient of the bedrock structural features. Geologic structures in the bedrock are potential pathways for recharge to the DGDA from surrounding bedrock. [source] Physical fitness in children with haemophilia and the effect of overweightHAEMOPHILIA, Issue 2 2009D. C. M. DOUMA-VAN RIET Summary., Although children with haemophilia are advised to participate in physical activities, their physical fitness has not been studied in a large group. In addition, children with haemophilia may be at increased risk for becoming overweight as a result of inactivity because of joint bleedings or because of overprotection. This study aimed to assess physical fitness (aerobic capacity), joint status, muscle strength, quality of life (QoL), self-reported motor competence and also prevalence of overweight and its association with physical parameters. Weight and height were measured. Skin folds were measured unilaterally at biceps, triceps, subscapular and supra-iliac sites. Aerobic capacity was determined on a cycle ergometer or with a 6-min walk test (6MWT). Muscle strength and active range of motion of elbows, knees and ankle joints were measured. Self-reported motor competence was measured with the ,Competentie BelevingsSchaal voor Kinderen'. Joint pain was scored on a Visual Analogue Scale. The Haemo-QoL Index was used to measure QoL. In 158 Dutch boys with haemophilia, with a mean age of 12.7 years (SD 2.9), normal aerobic capacity and muscle strength were found. Joint pain was reported by 16% of the participants. The prevalence of overweight (16%) was slightly increased when compared with healthy Dutch boys (13.5%). Being overweight had a negative association with the 6MWT and QoL. Dutch children with haemophilia have normal aerobic exercise capacity and muscle strength. The majority also has normal joint mobility. Prevalence of overweight is slightly increased. [source] Water quality and Cryptosporidium distribution in an upland water supply catchment, Cumbria, UKHYDROLOGICAL PROCESSES, Issue 7 2007A. Sturdee Abstract Four micro-catchment (MC) areas were identified to represent the main terrain types of a remote, sparsely populated upland valley catchment of 18 km2 in Cumbria, UK. These were improved land with good grazing (IB), steeply sloping land with rough grazing (SG), wet moorland with sparse grazing (WM) and enclosed woodland that excluded livestock and deer (EW). Each MC contained the origin of a small stream that flowed into Swindale Beck, the river draining the valley. The water quality during the 14-month study, as judged by chemical and physical parameters, was excellent, but it could not be regarded as pristine because of the frequent presence of Cryptosporidium oocysts arising from livestock and wild mammal faeces. Oocysts (0·2,5·6 l,1) detected by genus-specific immunofluorescent antibody were found in 32% of 188 water samples tested: ranking order EW 44%, IB 34%, Beck 30%, SG and WM 26%. Similarly, oocysts were identified in 9·5% of 1730 faecal samples. Small wild mammals (28%), calves (15·7%) and lambs (8·1%) were the dominant sources, whereas adult livestock (1·8%) and large wild mammals (4·8%) were less important. Autumn showed the highest occurrence of oocysts for both water and faecal samples. No hydrological controls were found to have a significant impact on the occurrence or concentration of oocysts in the main river or in the MCs, suggesting that their presence is controlled by seasonal changes in pathogen prevalence in the animal reservoir. Copyright © 2006 John Wiley & Sons, Ltd. [source] Factors governing the formation and persistence of layers in a subalpine snowpackHYDROLOGICAL PROCESSES, Issue 7 2004David Gustafsson Abstract The layered structure of a snowpack has a great effect on several important physical processes, such as water movement, reflection of solar radiation or avalanche release. Our aim was to investigate what factors are most important with respect to the formation and persistence of distinct layers in a subalpine environment. We used a physically based numerical one-dimensional model to simulate the development of a snowpack on a subalpine meadow in central Switzerland during one winter season (1998,99). A thorough model validation was based on extensive measurement data including meteorological and snow physical parameters. The model simulated the snow water equivalent and the depth of the snowpack as well as the energy balance accurately. The observed strong layering of the snowpack, however, was not reproduced satisfactorily. In a sensitivity analysis, we tested different model options and parameter settings significant for the formation of snow layers. The neglection of effects of snow microstructure on the compaction rate, and the current description of the water redistribution inside the snowpack, which disregard capillary barrier effects, preferential flow and lateral water flow, were the major limitations for a more realistic simulation of the snowpack layering. Copyright © 2004 John Wiley & Sons, Ltd. [source] Root canal preparation with FlexMaster: asessment of torque and force in relation to canal anatomyINTERNATIONAL ENDODONTIC JOURNAL, Issue 12 2003W. Hübscher Abstract Aim, To investigate physical parameters of FlexMaster nickel-titanium instruments while preparing curved canals in maxillary molars in vitro. Methodology, A torque-testing platform was used to prepare root canals in 11 extracted human maxillary molars with FlexMaster rotary instruments. Peak torque and force was registered along with numbers of rotations required to shape the canals. Canals were divided into ,wide' and ,constricted' groups depending on canal volumes assessed by microcomputed tomography. Resistance to cyclic fatigue was also tested. Mean scores for each instrument type were calculated and statistically compared using anova and Scheffé post hoc tests. Results, Mean torque varied between 0.1 ± 0.1 and 0.8 ± 0.5 N cm while mean force ranged from 4.2 ± 2.0 to 7.3 ± 3.5 N. Mean numbers of rotations totalled up to 18. All three variables registered showed weak correlations to preoperative canal volumes (P < 0.01) and differed significantly between ,wide' and ,constricted' canals (P < 0.001). Numbers of rotations to fracture in a cyclic fatigue test were between 348 and 1362. Conclusion, FlexMaster instruments generated low torque scores and were highly resistant to cyclic fatigue, whilst three instruments fractured in extremely narrow canals. Consequently, more research is required to limit fracture incidence and to optimize instrumentation guidelines. [source] Flow of a third grade fluid due to an accelerated diskINTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN FLUIDS, Issue 8 2010S. Asghar Abstract The magnetohydrodynamic (MHD) flow induced by non-coaxial rotation of porous disk and a third grade fluid at infinity is investigated. The disk is moving with uniform acceleration and rotating with a uniform angular velocity. Numerical solution of the governing nonlinear initial and boundary value problem is obtained. The effects of physical parameters on the velocity profiles are examined in detail. The present study shows that the constant acceleration part has a greater influence than the time part of the assumed variable velocity of the disk. Copyright © 2009 John Wiley & Sons, Ltd. [source] Finite-element/level-set/operator-splitting (FELSOS) approach for computing two-fluid unsteady flows with free moving interfacesINTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN FLUIDS, Issue 3 2005Anton Smolianski Abstract The present work is devoted to the study on unsteady flows of two immiscible viscous fluids separated by free moving interface. Our goal is to elaborate a unified strategy for numerical modelling of two-fluid interfacial flows, having in mind possible interface topology changes (like merger or break-up) and realistically wide ranges for physical parameters of the problem. The proposed computational approach essentially relies on three basic components: the finite element method for spatial approximation, the operator-splitting for temporal discretization and the level-set method for interface representation. We show that the finite element implementation of the level-set approach brings some additional benefits as compared to the standard, finite difference level-set realizations. In particular, the use of finite elements permits to localize the interface precisely, without introducing any artificial parameters like the interface thickness; it also allows to maintain the second-order accuracy of the interface normal, curvature and mass conservation. The operator-splitting makes it possible to separate all major difficulties of the problem and enables us to implement the equal-order interpolation for the velocity and pressure. Diverse numerical examples including simulations of bubble dynamics, bifurcating jet flow and Rayleigh,Taylor instability are presented to validate the computational method. Copyright © 2004 John Wiley & Sons, Ltd. [source] | ||||||||||||||||||||||||||||||||||||||||