Home About us Contact | |||
Photographic Film (photographic + film)
Selected AbstractsDigital photography: A primer for pathologistsJOURNAL OF CLINICAL LABORATORY ANALYSIS, Issue 2 2004Roger S. Riley Abstract The computer and the digital camera provide a unique means for improving hematology education, research, and patient service. High quality photographic images of gross specimens can be rapidly and conveniently acquired with a high-resolution digital camera, and specialized digital cameras have been developed for photomicroscopy. Digital cameras utilize charge-coupled devices (CCD) or Complementary Metal Oxide Semiconductor (CMOS) image sensors to measure light energy and additional circuitry to convert the measured information into a digital signal. Since digital cameras do not utilize photographic film, images are immediately available for incorporation into web sites or digital publications, printing, transfer to other individuals by email, or other applications. Several excellent digital still cameras are now available for less than $2,500 that capture high quality images comprised of more than 6 megapixels. These images are essentially indistinguishable from conventional film images when viewed on a quality color monitor or printed on a quality color or black and white printer at sizes up to 11×14 inches. Several recent dedicated digital photomicroscopy cameras provide an ultrahigh quality image output of more than 12 megapixels and have low noise circuit designs permitting the direct capture of darkfield and fluorescence images. There are many applications of digital images of pathologic specimens. Since pathology is a visual science, the inclusion of quality digital images into lectures, teaching handouts, and electronic documents is essential. A few institutions have gone beyond the basic application of digital images to developing large electronic hematology atlases, animated, audio-enhanced learning experiences, multidisciplinary Internet conferences, and other innovative applications. Digital images of single microscopic fields (single frame images) are the most widely utilized in hematology education at this time, but single images of many adjacent microscopic fields can be stitched together to prepare "zoomable" panoramas that encompass a large part of a microscope slide and closely simulate observation through a real microscope. With further advances in computer speed and Internet streaming technology, the virtual microscope could easily replace the real microscope in pathology education. Later in this decade, interactive immersive computer experiences may completely revolutionize hematology education and make the conventional lecture and laboratory format obsolete. Patient care is enhanced by the transmission of digital images to other individuals for consultation and education, and by the inclusion of these images in patient care documents. In research laboratories, digital cameras are widely used to document experimental results and to obtain experimental data. J. Clin. Lab. Anal. 18:91,128, 2004. © 2004 Wiley-Liss, Inc. [source] Application of an enhanced luminol chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H -imidazol-2-yl]phenylboronic acid to photographic detection of horseradish peroxidase on a membraneLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 2 2001Naotaka Kuroda Abstract Photographic detection of horseradish peroxidase (HRP) on a membrane by the luminol,hydrogen peroxide,HRP chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H -imidazol-2-yl]phenylboronic acid (DPPA) as an enhancer is described. The method is based on the long-lived chemiluminescence emission obtained by using DPPA. Under the optimum conditions, as little as 0.10,ng (ca. 2.3 fmol) and 0.20,ng (ca. 4.6 fmol) per spot of HRP on a membrane were detected as visible spots with exposure time of 60 and 10,min, respectively, by using an instant photographic film and a camera luminometer. The proposed method was highly sensitive and was successfully applied to the detection of HRP conjugates as an alternative to the colorimetric method using a chromogenic substrate in a commercially available assay kit of Western blotting. Copyright © 2001 John Wiley & Sons, Ltd. [source] Ophthalmic imaging today: an ophthalmic photographer's viewpoint , a reviewCLINICAL & EXPERIMENTAL OPHTHALMOLOGY, Issue 1 2009Timothy J Bennett FOPS Abstract Ophthalmic imaging has changed dramatically since the 1960s with increasingly complex technologies now available. Arguably, the greatest changes have been the development of the digital camera and the speed, processing power and storage of electronic data. Already, ophthalmic practices in many major institutions overseas have paperless medium storage and electronically generated reporting from all equipment that use a computer interface. It is hard to remember the widespread use of photographic film with its attendant costs, or even to remember the days before optical coherence tomography (OCT). These latest technical improvements in ophthalmic imaging are now standard in large Australian institutions and becoming more widespread in smaller private practices. The technicians that operate and maintain this ever-increasing plethora of gadgetry have seen their work practices change from the darkroom to the complexities of data-based imaging and storage. It is a fitting time to examine the contemporary state of ophthalmic imaging and what lies on the horizon as we move towards 2020. [source] Mixed Aza-Thioether Crowns Containing a 1,10-Phenanthroline Sub-Unit as Neutral Ionophores for Silver IonELECTROANALYSIS, Issue 24 2002Mojtaba Shamsipur Abstract Three different recently synthesized aza-thioether crowns containing a 1,10-phenanthroline sub-unit (L1,L3) and a corresponding acyclic ligand (L4) were studied to characterize their abilities as silver ion ionophores in PVC-membrane electrodes. Novel conventional silver-selective electrodes with internal reference solution (CONISE) and coated graphite-solid contact electrodes (SCISE) were prepared based on one of the 15-membered crowns containing two donating S atoms and two phenanthroline-N atoms (L1). The electrodes reveal a Nernstian behavior over wide Ag+ ion concentration ranges (1.0×10,5,1.0×10,1,M for CONISE and 5.0×10,8,4.0×10,2,M for SCISE) and very low limits of detection (8.0×10,6,M for CONISE and 3.0×10,8,M for SCISE). The potentiometric response is independent from pH of the solution in the pH range 3.0,8.0. The electrodes manifest advantages of low resistance, very fast response and, most importantly, good selectivities relative to a wide variety of other cations. The electrodes can be used for at least 2 months (for CONISE) and 4 months for (SCISE) without any appreciable divergence in potentials. The electrodes were used as an indicator electrode in the potentiometric titration of Ag+ ion and in the determination of silver in photographic emulsions and in radiographic and photographic films. [source] Elastohydrodynamics of tensioned web roll coating processINTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN FLUIDS, Issue 6 2003M. S. Carvalho Abstract Coating process is an important step in the manufacturing of different products, such as paper, adhesive and magnetic tapes, photographic films, and many other. The tensioned web roll coating is one the several methods used by different industries. It relies on the elastohydrodynamic action between the fluid and the tensioned substrate for transferring and applying the liquid. The main advantage of this method is its ability to apply very thin liquid layers with less sensitivity to mechanical tolerance at relative small cost. Despite its industrial application, theoretical analysis and fundamental understanding of the process are limited. This work analyses this elastohydrodynamic action by solving the differential equations that govern the liquid flow, described by the Navier,Stokes equation, and the web deformation, modelled by the cylindrical shell approximation. The goal is to determine the operating conditions at which the process is two dimensional and defect free. The equations are discretized by the Galerkin/finite-element method. The resulting non-linear system of equations is solved by Newton's method coupled with pseudo-arc-length continuation in order to obtain solutions around turning points. The theoretical results are used to construct an operating window of the process that is in agreement with limited experimental data. Copyright © 2003 John Wiley & Sons, Ltd. [source] |