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Phospholipid Fraction (phospholipid + fraction)
Selected AbstractsGrowth, fat content and fatty acid profile of South American catfish, surubim (Pseudoplatystoma fasciatum) juveniles fed live, commercial and formulated dietsJOURNAL OF APPLIED ICHTHYOLOGY, Issue 1 2009M. Arslan Summary South American catfish, barred surubim (Pseudoplatystoma fasciatum) juveniles (117.6 ± 11.8 mg individual weight; 28.3 ± 2.5 mm total length) were fed various diets: one live (Tubifex worms), two commercial (Aglo Norse and Bio Kyowa), and one semi-purified formulated diet (75% peptide based protein) over a 2-week period. Fish fed the Aglo Norse diet showed the highest growth performance, but cannibalism also was very high (42%). Fish fed peptide based formulated diet demonstrated the lowest growth rate, with no cannibalism. The highest survival was achieved with fish fed Tubifex worms (100%). Lipid level in the whole body of the fish fed four different experimental diets did not differ significantly, averaging 3.6 ± 0.7%. Fatty acid composition of neutral and phospholipid fractions of whole body lipids of fish reflected the fatty acid composition of the diets. The high level of 20:4n -6 in Tubifex worms resulted in a high level of this fatty acid in the tissue of fish fed this diet. It remains uncertain how high survival and no cannibalism is related to dietary lipids/fatty acids. In all cases, the increasing ratio of n -3 HUFA (highly unsaturated fatty acids)/n -6 HUFA in phospholipid fractions suggested the elongation and desaturation of 18:3n -3 to 22:6n -3 via 20:5n -3. Moreover, in respect to the 20:4n -6 levels in the diets, an increase in the concentration of this fatty acid in phospholipid fraction suggests that South American catfish can transform linoleate into arachidonate. [source] Phytanic Acid Accumulation Is Associated with Conduction Delay and Sudden Cardiac Death in Sterol Carrier Protein-2/Sterol Carrier Protein-x Deficient MiceJOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY, Issue 11 2004GEROLD MÖNNIG M.D. Introduction: The sterol carrier protein-2 gene encodes two functionally distinct proteins: sterol carrier protein-2 (SCP2, a peroxisomal lipid carrier) and sterol carrier protein-x (SCPx, a peroxisomal thiolase known as peroxisomal thiolase-2), which is involved in peroxisomal metabolism of bile acids and branched-chain fatty acids. We show in this study that mice deficient in SCP2 and SCPx (SCP2null) develop a cardiac phenotype leading to a high sudden cardiac death rate if mice are maintained on diets enriched for phytol (a metabolic precursor of branched-chain fatty acids). Methods and Results: In 210 surface and 305 telemetric ECGs recorded in wild-type (C57BL/6; wt; n = 40) and SCP2 null mice (n = 40), no difference was observed at baseline. However, on diet, cycle lengths were prolonged in SCP2 null mice (262.9 ± 190 vs 146.3 ± 43 msec), AV conduction was prolonged (58.3 ± 17 vs 42.6 ± 4 ms), and QRS complexes were wider (19.1 ± 5 vs 14.0 ± 4 ms). In 11 gene-targeted Langendorff-perfused hearts isolated from SCP2 null mice after dietary challenge, complete AV blocks (n = 5/11) or impaired AV conduction (Wenckebach point 132 ± 27 vs 92 ± 10 msec; P < 0.05) could be confirmed. Monophasic action potentials were not different between the two genotypes. Left ventricular function studied by echocardiography was similar in both strains. Phytanic acid but not pristanic acid accumulated in the phospholipid fraction of myocardial membranes isolated from SCP2 null mice. Conclusion: Accumulation of phytanic acid in myocardial phospholipid membranes is associated with bradycardia and impaired AV nodal and intraventricular impulse conduction, which could provide an explanation for sudden cardiac death in this model. [source] Long chain-polyunsaturated fatty acids modulate membrane phospholipid composition and protein localization in lipid rafts of neural stem cell culturesJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 6 2010Bénédicte Langelier Abstract Rat neural stem cells/neural progenitors (NSC/NP) are generally grown in serum-free medium. In this study, NSC/NP were supplemented with the main long-chain polyunsaturated fatty acids (PUFAs) present in the brain, arachidonic acid (AA), or docosahexaenoic acid (DHA), and were monitored for their growth. Lipid and fatty acid contents of the cells were also determined. Under standard conditions, the cells were characterized by phospholipids displaying a highly saturated profile, and very low levels of PUFAs. When cultured in the presence of PUFAs, the cells easily incorporated them into the phospholipid fraction. We also compared the presence of three membrane proteins in the lipid raft fractions: GFR and connexin 43 contents in the rafts were increased by DHA supplementation, whereas G, subunit content was not significantly modified. The restoration of DHA levels in the phospholipids could profoundly affect protein localization and, consequently, their functionalities. J. Cell. Biochem. 110: 1356,1364, 2010. © 2010 Wiley-Liss, Inc. [source] Macrophages transfer [14C]-labelled fatty acids to pancreatic islets in cultureCELL BIOCHEMISTRY AND FUNCTION, Issue 1 2001J. R. Garcia Jr. Abstract Macrophages are able to produce, export, and transfer fatty acids to lymphocytes in culture. The purpose of this study was to examine if labelled fatty acids could be transferred from macrophages to pancreatic islets in co-culture. We found that after 3,h of co-culture the transfer of fatty acids to pancreatic islets was: arachidonic , oleic > linoleic,=,palmitic. Substantial amounts of the transferred fatty acids were found in the phospholipid fraction; 87.6% for arachidonic, 59.9% for oleic, 53.1% for palmitic, and 36.9% for linoleic acids. The remaining radioactivity was distributed among the other lipid fractions analysed (namely polar lipids, cholesterol, fatty acids, triacylglycerol and cholesterol ester), varying with the fatty acid used. For linoleic acid, a significant proportion (63.1%) was almost equally distributed in these lipid fractions. Also, it was observed that transfer of fatty acids from macrophages to pancreatic islets is time-dependent up to 24,h, being constant and linear with time for palmitic acid and remaining constant after 12,h for oleic acid. These results lead us to postulate that in addition to the serum, circulating monocytes may also be a source of fatty acids to pancreatic islets, mainly arachidonic acid. Copyright © 2001 John Wiley & Sons, Ltd. [source] The fatty acid compositions of predator Piocoris luridus (Heteroptera: Lygaeidae) and its host Monosteria unicostata (Heteroptera: Tingidae) reared on almondINSECT SCIENCE, Issue 6 2007OZLEM CAKMAK Abstract The changes in fatty acid compositions during nutritional interaction among almond Amygdalus communis Linnaeus (Rosales: Rosaceae) (host plant), lacebug Monosteria unicostata (Mulsant and Rey) (Heteroptera: Tingidae) and its predator Piocoris luridus Fieber (Heteroptera: Lygaeidae) were determined by gas chromatography and gas chromatography-mass spectrometry analyses. The fatty acid profiles of phospholipids and triacylglycerols were substantially different. Unlike the general observations for virtually most terrestrial insects, arachidonic and eicosapentaenoic acids were detected in high proportions of phospholipid fractions in both insects, especially in P. luridus. Also the almond tissues provide very little oleic acid to the herbivore diet, yet both insect species developed high proportions of this component. Our data reveals instances of specific accumulation of fatty acid biosynthesis, elongation/desaturation, and not incorporating selected fatty acids into cellular lipids. [source] Effect of cream treatment on phospholipids and protein recovery in butter-making processINTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 4 2008Michel Britten Summary A simple approach is proposed to recover native protein and phospholipid fractions from butter-making process using equipments available in dairy-processing plant. A washing treatment was used to remove protein from the cream and increase the phospholipids purity in buttermilk. Cream from a first separation was diluted with milk ultrafitration permeate and separated a second time. A 10X dilution factor reduced protein concentration in the cream from 1.6 ± 0.2 to 0.52 ± 0.03%. As a result, the phospholipids to protein ratio in buttermilk increased from 53 ± 10 to 172 ± 7 mg g,1. In butter-making process, an important portion of total phospholipids (,26%) is retained in butter. Butter serum made from washed cream could then be used to produce phospholipid concentrates with phospholipids to protein ratio of 473 ± 3 mg g,1. Interestingly, the extracts from butter serum are characterised by a higher proportion of sphingomyelin compared with those from buttermilk. [source] Growth, fat content and fatty acid profile of South American catfish, surubim (Pseudoplatystoma fasciatum) juveniles fed live, commercial and formulated dietsJOURNAL OF APPLIED ICHTHYOLOGY, Issue 1 2009M. Arslan Summary South American catfish, barred surubim (Pseudoplatystoma fasciatum) juveniles (117.6 ± 11.8 mg individual weight; 28.3 ± 2.5 mm total length) were fed various diets: one live (Tubifex worms), two commercial (Aglo Norse and Bio Kyowa), and one semi-purified formulated diet (75% peptide based protein) over a 2-week period. Fish fed the Aglo Norse diet showed the highest growth performance, but cannibalism also was very high (42%). Fish fed peptide based formulated diet demonstrated the lowest growth rate, with no cannibalism. The highest survival was achieved with fish fed Tubifex worms (100%). Lipid level in the whole body of the fish fed four different experimental diets did not differ significantly, averaging 3.6 ± 0.7%. Fatty acid composition of neutral and phospholipid fractions of whole body lipids of fish reflected the fatty acid composition of the diets. The high level of 20:4n -6 in Tubifex worms resulted in a high level of this fatty acid in the tissue of fish fed this diet. It remains uncertain how high survival and no cannibalism is related to dietary lipids/fatty acids. In all cases, the increasing ratio of n -3 HUFA (highly unsaturated fatty acids)/n -6 HUFA in phospholipid fractions suggested the elongation and desaturation of 18:3n -3 to 22:6n -3 via 20:5n -3. Moreover, in respect to the 20:4n -6 levels in the diets, an increase in the concentration of this fatty acid in phospholipid fraction suggests that South American catfish can transform linoleate into arachidonate. [source] | ||||||||||