			Home About us Contact

Phosphate Nanoparticles (phosphate + nanoparticle)

Distribution by Scientific Domains

Polymers and Materials Science	50%

Selected Abstracts

Coating of Human Mesenchymal Cells in 3D Culture with Bioinorganic Nanoparticles Promotes Osteoblastic Differentiation and Gene Transfection,

ADVANCED MATERIALS, Issue 17 2007
R. Gonzalez-McQuire
Mesenchymal cells are modified in suspension with bio-functionalized calcium phosphate nanoparticles using a scaffold-free cell decoration method. Coated cells remain viable and retain functionality, with cell activity stimulated by the structure and composition of the coating. [source]

Nanogel-Templated Mineralization: Polymer-Calcium Phosphate Hybrid Nanomaterials

MACROMOLECULAR RAPID COMMUNICATIONS, Issue 6 2006
Ayae Sugawara
Abstract Summary: We report novel organic-inorganic hybrid nanomaterials that consist of polymer hydrogel nanoparticles (nanogels) and calcium phosphate. Hybrid nanoparticles that measure ca. 40 nm are synthesized from a dilute solution of hydroxyapatite using nanogels as templates for calcium phosphate mineralization. These nanoparticles show a narrow size distribution and high colloidal stability. Nanogel-adsorbed liposomes act as templates for hierarchical hybrid nanostructures. These nanohybrids can potentially be used as biocompatible drug carriers with controlled-release properties. TEM images of calcium phosphate nanoparticles formed in the presence of CHP nanogels (0.5 mg,·,mL,1) (left) and nanogel-liposomes (CHP 0.05 mg,·,mL,1, DPPC 0.08 mg,·,mL,1)(right). [source]

Tricalcium phosphate nanoparticles enable rapid purification, increase transduction kinetics, and modify the tropism of mammalian viruses

BIOTECHNOLOGY & BIOENGINEERING, Issue 4 2009
Imke A.J. Dreesen
Abstract Adenoviral, adeno-associated viral, and retroviral particles are chosen as gene delivery shuttles in more than 50% of all gene therapy clinical trials. Bulk availability of clinical-grade viral particles and their efficiency to transduce the therapeutic cargo into specific target cells remain the most critical bottlenecks in gene therapy applications to date. Capitalizing on the flame-spray technology for the reproducible economic large-scale production of amorphous tricalcium phosphate nanoparticulate powders (ATCP), we designed a scalable ready-to-use gravity-flow column set-up for the straightforward concentration and purification of transgenic adenoviral, adeno-associated viral, and lentiviral particles. Specific elution buffers enabled rapid release of viral particles from the ATCP matrix of the column and provided high-titer virus preparations in an unsurpassed period of time. The interaction of ATCP with adenoviral, adeno-associated viral, and lentiviral particles in solution increased the transduction kinetics of several mammalian cell lines in culture. The nanoparticles were also able to modify the tropism of murine leukemia virus (MLV) towards transduction of human cells. Based on these findings, we believe that the use of flame-spray tricalcium phosphate nanoparticles will lead to important progress in the development of future gene therapy initiatives. Biotechnol. Bioeng. 2009;102: 1197,1208. © 2008 Wiley Periodicals, Inc. [source]