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Phase Method (phase + method)
Selected AbstractsPiezoelectric field around threading dislocation in GaN determined on the basis of high-resolution transmission electron microscopy imageJOURNAL OF MICROSCOPY, Issue 3 2006G. MACIEJEWSKI Summary A new method of determining the piezoelectric field around dislocations from high-resolution transmission electron microscopy images is presented. In order to determine the electrical potential distribution near a dislocation core, we used the distortion field, obtained using the geometrical phase method and the non-linear finite element method. The electrical field distribution was determined taking into account the inhomogeneous strain distribution, finite geometry of the sample and the full couplings between elastic and electrical fields. The results of the calculation for a transmission electron microscopy thin sample are presented. [source] Solid-phase biotinylation of antibodies,JOURNAL OF MOLECULAR RECOGNITION, Issue 3 2004Elizabeth Strachan Abstract Biotinylation is an established method of labeling antibody molecules for several applications in life science research. Antibody functional groups such as amines, cis hydroxyls in carbohydrates or sulfhydryls may be modified with a variety of biotinylation reagents. Solution-based biotinylation is accomplished by incubating antibody in an appropriate buffered solution with biotinylation reagent. Unreacted biotinylation reagent must be removed via dialysis, diafiltration or desalting. Disadvantages of the solution-based approach include dilution and loss of antibody during post-reaction purification steps, and difficulty in biotinylation and recovery of small amounts of antibody. Solid-phase antibody biotinylation exploits the affinity of mammalian IgG-class antibodies for nickel IMAC (immobilized metal affinity chromatography) supports. In this method, antibody is immobilized on a nickel-chelated chromatography support and derivitized on-column. Excess reagents are easily washed away following reaction, and biotinylated IgG molecule is recovered under mild elution conditions. Successful solid phase labeling of antibodies through both amine and sulfhydryl groups is reported, in both column and mini-spin column formats. Human or goat IgG was bound to a Ni-IDA support. For sulfhydryl labeling, native disulfide bonds were reduced with TCEP, and reduced IgG was biotinylated with maleimide,PEO2 biotin. For amine labeling, immobilized human IgG was incubated with a solution of NHS,PEO4 biotin. Biotinylated IgG was eluted from the columns using a buffered 0.2,M imidazole solution and characterized by ELISA, HABA/avidin assay, probing with a streptavidin,alkaline phosphatase conjugate, and binding to a monomeric avidin column. The solid phase protocol for sulfhydryl labeling is significantly shorter than the corresponding solution phase method. Biotinylation in solid phase is convenient, efficient and easily applicable to small amounts of antibody (e.g. 100,,g). Antibody biotinylated on-column was found to be equivalent in stability and antigen-recognition ability to antibody biotinylated in solution. Solid-phase methods utilizing Ni-IDA resin have potential for labeling nucleic acids, histidine-rich proteins and recombinant proteins containing polyhistidine purification tags, and may also be applicable for other affinity systems and labels. Copyright © 2004 John Wiley & Sons, Ltd. [source] Return mapping of phases and the analysis of the gravitational clustering hierarchyMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2002Lung-Yih Chiang ABSTRACT In the standard paradigm for cosmological structure formation, clustering develops from initially random-phase (Gaussian) density fluctuations in the early Universe by a process of gravitational instability. The later, non-linear stages of this process involve Fourier mode,mode interactions that result in a complex pattern of non-random phases. We present a novel mapping technique that reveals mode coupling induced by this form of non-linear interaction and allows it to be quantified statistically. The phase mapping technique circumvents the difficulty of the circular characteristic of ,k and illustrates the statistical significance of phase difference at the same time. This generalized phase method allows us to detect weak coupling of phases on any ,k scales. [source] Preparation of lithium indium oxide via a rheological phase route and its electrochemical characteristics in LiOH and Li2SO4 solutionsPHYSICA STATUS SOLIDI (A) APPLICATIONS AND MATERIALS SCIENCE, Issue 1 2010Guo-Qing Zhang Abstract Submicrometer-sized lithium indium oxide (LiInO2) powder via a rheological phase method using trilithium citrate tetra hydrate (C6H5Li3O7,·,4H2O) and indium oxide (In2O3) has been prepared in this work for the first time. The optimal pyrolyzing temperature range to prepare crystalline LiInO2 is between 650 and 900,°C, which was confirmed by thermal gravimetric and differential thermogravimetric analysis of the precursor and X-ray diffraction analysis. The pure phase LiInO2 sample obtained has a uniform particle morphology and submicrosize, which was observed by scanning electron microscopy. The electrochemical studies show that a new pair of cathodic and anodic peaks at 0.23 and 0.38,V (vs. saturated calomel electrode) was obviously observed from the cyclic voltammetry curve of LiInO2 in 1,M LiOH solution, indicating a battery characteristic of the material in this electrolyte. While in 1,M Li2SO4 solution, the sample presents a supercapacitive characteristic within the same potential range. The reasons for different electrochemical behaviors in these two electrolytes can be attributed to the fact that the reaction of lithium ion insertion/extraction into/out of a LiInO2 electrode takes place in the bulk material in LiOH electrolyte solution, whereas it takes place on the electrode/electrolyte interface for Li2SO4 electrolyte case. [source] Fabrication of field emitters using GaN particlesPHYSICA STATUS SOLIDI (C) - CURRENT TOPICS IN SOLID STATE PHYSICS, Issue 7-8 2010Yuichiro Minakuchi Abstract The field emission from GaN particles has been demonstrated successfully for the first time. The novel field emitting device has a simple structure consisting of an electrically conductive polymer layer and GaN particles spread randomly on it. The GaN particles used for this device, which were synthesized in advance by the two-stage vapor phase method, have vertices and ridges formed by well-developed crystal planes. The electron emission started at an electric field of about 20 V/,m, and the current reached 10 nA at 28 V/,m. The maximum current density was 0.26 mA/cm2at 43 V/,m. The Fowler-Nordheim (F-N) plot of the I - V data indicated that the observed electron emission is originated from the F-N tunneling. (© 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Movement of the tongue during normal breathing in awake healthy humansTHE JOURNAL OF PHYSIOLOGY, Issue 17 2008S. Cheng Electromyographic (EMG) activity of the airway muscles suggest that genioglossus is the primary upper airway dilator muscle. However, EMG data do not necessarily translate into tissue motion and most imaging modalities are limited to assessment of the surfaces of the upper airway. In this study, we hypothesized that genioglossus moves rhythmically during the respiratory cycle and that the motion within is inhomogeneous. A ,tagged' magnetic resonance imaging technique was used to characterize respiratory-related tissue motions around the human upper airway in quiet breathing. Motion of airway tissues at different segments of the eupnoeic respiratory cycle was imaged in six adult subjects by triggering the scanner at the end of inspiration. Displacements of the ,tags' were analysed using the harmonic phase method (HARP). Respiratory timing was monitored by a band around the upper abdomen. The genioglossus moved during the respiratory cycle. During expiration, the genioglossus moved posteriorly and during inspiration, it moved anteriorly. The degree of motion varied between subjects. The maximal anteroposterior movement of a point tracked on the genioglossus was 1.02 ± 0.54 mm (mean ±s.d.). The genioglossus moved over the geniohyoid muscle, with minimal movement in other muscles surrounding the airway at the level of the soft palate. Local deformation of the tongue was analysed using two-dimensional strain maps. Across the respiratory cycle, positive strains within genioglossus reached peaks of 17.5 ± 9.3% and negative strains reached peaks of ,16.3 ± 9.3% relative to end inspiration. The patterns of strains were consistent with elongation and compression within a constant volume structure. Hence, these data suggest that even during respiration, the tongue behaves as a muscular hydrostat. [source] Vibrational infrared conformational studies of model peptides representing the semicrystalline domains of Bombyx mori silk fibroin,BIOPOLYMERS, Issue 5 2005Paola Taddei Abstract The structural organization of Bombyx mori silk fibroin was investigated by infrared (IR) spectroscopy. To this aim, (AG)15 and other model peptides of varying chain length, containing tyrosine (Y), valine (V), and serine (S) in the basic (AG)n sequence were synthesized by the solid phase method and their spectroscopic properties were determined. Both the position and the relative content of Y, V, and S residues in the (AG)n model system appeared critical in determining the preferred conformation, i.e., silk I, silk II, and unordered structures. Curve fitting analysis in the amide I range showed that the model peptides with prevailing silk II structure displayed different ,-sheet content, which was dependent on the degree of interruption of the (AG)n sequence. In this regard, the bands at about 1000 and 980 cm,1, specifically assigned to the AG sequence of the B. mori silk fibroin chain, were identified as marker of the degree of interruption of the (AG)n sequence. A stable silk I structure was observed only when the Y residue was located near the chain terminus, while a silk I , silk II conformational transition occurred when it was positioned in the central region of the peptide. Analysis of the second-derivative spectra in the amide I range allowed us to identify a band at 1639 cm,1 (4 , 1 hydrogen-bonded type II ,-turns), which is characteristic of the silk I conformation. © 2005 Wiley Periodicals, Inc. Biopolymers 78: 249,258, 2005 [source] | |||||||||||||