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Pharmaceutical Analysis (pharmaceutical + analysis)
Selected AbstractsCover Picture: Electrophoresis 17/2008ELECTROPHORESIS, Issue 17 2008Article first published online: 19 SEP 200 Issue no. 17 is a Special Issue on "Pharmaceutical Analysis" consisting of 25 papers that are arranged into five parts. This issue contains reviews on impurity profiling of pharmaceuticals, quality control of herbal medicines, and quality assurance of the glycosylation of antibodies. Research papers illustrate examples of enantioseparations, protein and macromolecule analysis, drug impurity profiling, determination of enzymatic activity by CE, and bioanalysis. [source] JPAG Session: Short Papers in Pharmaceutical AnalysisJOURNAL OF PHARMACY AND PHARMACOLOGY: AN INTERNATI ONAL JOURNAL OF PHARMACEUTICAL SCIENCE, Issue S1 2008Article first published online: 18 FEB 2010 First page of article [source] Short Papers in Pharmaceutical Analysis and PharmacognosyJOURNAL OF PHARMACY AND PHARMACOLOGY: AN INTERNATI ONAL JOURNAL OF PHARMACEUTICAL SCIENCE, Issue S1 2004Article first published online: 18 FEB 2010 No abstract is available for this article. [source] Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2-ethylpyridine columnJOURNAL OF SEPARATION SCIENCE, JSS, Issue 8 2008Claudio Brunelli Abstract The separation of neutral, acidic, and basic pharmaceuticals with diverse physicochemical properties by packed column supercritical fluid chromatography (pSFC) on a 2-ethylpyridine column (25 cm×4.6 mm id, 3 ,m particles) is presented. The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open-access generic screening environments. [source] Cover Picture: Electrophoresis 7'2010ELECTROPHORESIS, Issue 7 2010Article first published online: 26 MAR 2010 Issue no. 7 is a special issue on CE-MS consisting of "19 manuscripts subdivided into three major categories: one devoted to instrumental and methodical advances, two providing an insight into up-to-date applications from the fields of technical and natural products, food and environmental analysis on the one hand and biomedical and pharmaceutical analysis on the other hand. Diverse approaches how CE-MS can be employed for the solution of various analytical problems can be found in these papers. Different modes of electroseparation techniques in the capillary format such as CZE, CEC or MEEKC are coupled to various MS instruments ranging from simple quadrupole MS instruments to state of the art QTOF's, using a range of interfaces such as ESI, ICP or APPI. So this special issue will again try to present an overview of current trends and developments in the fields of CE-MS" [source] Cover Picture: Electrophoresis 10/2008ELECTROPHORESIS, Issue 10 2008Article first published online: 21 MAY 200 Regular issues provide a wide range of research and review articles covering all aspects of electrophoresis. Here you will find cutting-edge articles on methods and theory, instrumentation, nucleic acids, CE and CEC, miniaturization and microfluidics, proteomics and two-dimensional electrophoresis. "The present issue includes 29 manuscripts subdivided into three major parts: one part is devoted to instrumental and methodological advances, and two parts are providing an insight into up-to-date applications from the fields of natural products and food analysis on the one hand and biomedical and pharmaceutical analysis on the other hand. The approaches used comprise different modes of electroseparation methods such as CZE, packed column, monolithic column and open-tubular CEC, MEKC, CIEF, CITP, different modes of ionization such as MALDI, ICP, and ESI, as well as a range of mass analyzers from simple single quadrupole MS to top of the range Q-TOF instruments, providing MS-MS and accurate mass features." [source] Electrophoretic methods in pharmaceutical analysisELECTROPHORESIS, Issue 12 2006Hermann Wätzig No abstracts. [source] Large-volume sample stacking combined with separation by 2-hydroxypropyl-,-cyclodextrin for analysis of isoxyzolylpenicillins by capillary electrophoresisELECTROPHORESIS, Issue 17 2003Zhiwei Zhu Abstract A simple, quick and sensitive capillary electrophoretic technique has been developed for the pharmaceutical analysis of isoxazolylpenicillins (oxacillin, cloxacillin and dicloxacillin) at trace levels for the first time. This method comprises large-volume sample stacking using the electroosmotic flow (EOF) pump (LVSEP), separation using 2-hydroxypropyl-,-cyclodextrin (HP-,-CD) as selective complex-forming background electrolyte additive, and direct UV detection. A complete resolution was achieved in the optimal background electrolyte containing 5.2 mM HP-,-CD. LVSEP was successfully applied in their determinations to improve the sensitivity, where the EOF in the buffer zone was suppressed by using an acidic buffer with pH 3.6. The detection limits of the current technique were found to be 2.0 ,g/L for each of the isoxazolylpenicillins based on the signal-to-noise ratio of 3. The curves of peak response versus concentration were linear from 5.0 to 400.0 ,g/L with regression coefficients of 0.9982, 0.9986 and 0.9976, respectively. The interaction of isoxazolylpenicillins with HP-,-CD was discussed. The association constants for complexes of HP-,-CD with isoxazolylpenicillins were determined by electrophoretic method. The obtained association constants were 27.3, 34.9, and 48.5 M,1, respectively, being proportional to their hydrophobic properties and steric hindrances. A simple and easy-manipulative sample preparation method was developed and validated by analyzing commercially available milk samples. It was found that with current sample preparation process and instrumentation system, 0.1 mL of milk sample is enough for the analysis of isoxazolylpenicillins to meet European Union (EU) guideline of 30 ,g/kg. [source] Capillary electrochromatographic chiral separations with potential for pharmaceutical analysisJOURNAL OF SEPARATION SCIENCE, JSS, Issue 8 2005Debby Mangelings Abstract The use of capillary electrochromatography as a chiral separation technique for pharmaceutical applications is reviewed. Publications of the past 10 years that provide a potential practical application in pharmaceutical analysis are considered. Method development or validation, separation strategies, and potential routine analysis by the methods/applications cited are the main subjects on which we focused our attention. The indirect chiral separation method was only used once in CEC mode. In the direct chiral separations, the use of chiral stationary phases was obviously preferred over the use of chiral mobile phases with non-chiral stationary phases. Amongst the chiral stationary phases, those based on macrocyclic antibiotics and polysaccharide selectors were the most frequently used. Monolithic stationary phases also have several applications, but not so extended as those with packed capillary electrochromatography. The considered papers not only describe the applicability of the technique for relatively large sets of chiral analytes, they also showed that various types of stationary phases can be produced in-house in a simple manner. However, to survive as a mature separation technique, considerable time and effort are still needed to solve some disadvantages currently characterizing capillary electrochromatography. [source] Quantitative 1H NMR spectroscopic determination of the E/Z isomer ratio of the antidepressant drug fluvoxamine for use in pharmaceutical analysisMAGNETIC RESONANCE IN CHEMISTRY, Issue 12 2002Ralph Deubner Abstract High-resolution NMR spectroscopy is a powerful tool in the elucidation of a structure with respect to constitution, configuration and conformation. In recent years, NMR has been increasingly used in quantitative analysis. In this study, we show the ability of 1H NMR to determine the isomeric composition of the antidepressant drug fluvoxamine. The activity of fluvoxamine resides on the E -isomer, and the current British Pharmacopoeia limits the content of the Z -isomer to 0.5%. The NMR method described here is able to determine the content of the Z -isomer down to the 0.2% level on a total amount of 15 mg of the substance. Copyright © 2002 John Wiley & Sons, Ltd. [source] Sensitivity improvement of circular dichroism detection in HPLC by using a low-pass electronic noise filter: Application to the enantiomeric determination purity of a basic drugCHIRALITY, Issue 2 2007Marie Lorin Abstract The quality control of chiral drugs requires the determination of their enantiomeric purity. Nowadays, circular dichroism (CD) spectroscopy is gaining increasing importance in pharmaceutical analysis because of the commercially available CD detector in liquid chromatography. The separation of the two enantiomers of a basic drug (efaroxan) was achieved by high performance liquid chromatography using an amylose-derivated column with both UV and CD detections. A baseline-resolved separation (resolution: 5) was obtained after optimization of the mobile phase composition with hexane-ethanol-diethylamine (90:10:0.05; v/v/v). The use of a commercial low-pass electronic noise filter of the CD signal has improved the signal-to-noise ratio by a factor twelve and allowed the quantitation of each enantiomer in the 1.25,300 ,g ml,1 concentration range. The CD linear calibration curve, expressed in terms of stereoisomer height ratio versus concentration ratio, was plotted over the 0.4,6% range. A correlation coefficient greater than 0.999 was obtained by least-squares regression and the limit of detection for the distomer/eutomer ratio was estimated at 0.14%. Although the method validation showed good repeatability on the retention times (RSD < 0.9%), on the peak height ratios (RSD < 8.7%) of each enantiomer only up to 99.2% enantiomeric purity was achieved. Chirality, 2006. © 2006 Wiley-Liss, Inc. [source] | ||||||||||