Performance Thin-layer Chromatography (performance + thin-layer_chromatography)

Distribution by Scientific Domains

Kinds of Performance Thin-layer Chromatography

  • high performance thin-layer chromatography


  • Selected Abstracts


    Cover Picture: Electrophoresis 21'2009

    ELECTROPHORESIS, Issue 21 2009
    Article first published online: 27 OCT 200
    Issue no. 21 is a regular issue with Emphasis on "Nucleic Acids". The first part has 7 articles on nucleic acids covering various topics, e.g., sequencing, genotyping, PCR, insertion, mutation, etc. The remaining 11 articles are concerned with monoliths, pseudo-phases, coating, and sample pretreatment such as derivatization and concentration. Selected articles are: Applications of MALDI-TOF MS to large-scale human mtDNA population-based studies ((10.1002/elps.200900294)) Visual DNA as a diagnostic tool ((10.1002/elps.200900273)) Preliminary results for two-dimensional separation with high performance thin-layer chromatography and pressurized planar electrochromatography ((10.1002/elps.200900471)) [source]


    Quantitative determination of haloperidol in tablets by high performance thin-layer chromatography

    JOURNAL OF SEPARATION SCIENCE, JSS, Issue 5 2007
    Sigrid Mennickent
    Abstract A densitometric high performance thin-layer chromatography (HPTLC) method was developed and validated for the quantitative analysis of haloperidol in tablets. Chromatographic separation was achieved on precoated silica gel F 254 HPTLC plates using a mixture of acetone/chloroform/n -butanol/acetic acid glacial/water (5:10:10:2.5:2.5 v/v/v/v/v) as the mobile phase. Quantitative analysis was carried out at a wavelength of 254 nm. The method was linear in the 10,100 ng/,L range, with a determination coefficient of 0.999. The coefficients of variation for precision were not higher than 2.35%. The detection limit was 0.89 ng/,L, and the quantification limit was 2.71 ng/,L. The accuracy ranged from 97.76 to 100.33%, with a CV not higher than 4.50%. This method was successfully applied to quantify haloperidol in real pharmaceutical samples, including the comparison with HPLC measurements. The method was fast, specific, with a good precision and accuracy for the quantitative determination of haloperidol in tablets. [source]


    Retention data from reverse-phase high-performance thin-layer chromatography in characterization of some bis-salicylic acid derivatives

    BIOMEDICAL CHROMATOGRAPHY, Issue 8 2009
    -Sekuli, Tatjana Djakovi
    Abstract The chromatographic behaviour of salicylic acid derivatives was investigated using reversed-phase high performance thin-layer chromatography (RP HPTLC) with methanol,water and dioxane,water binary mixtures as mobile phase in order to establish relationships between chromatographic data and selected physico-chemical parameters that are related to ADME (absorption, distribution, metabolism and elimination). Some of the investigated compounds were screened for antioxidant activity. Examination of chromatographic behaviour revealed a linear correlation between RM values and the volume fraction of mobile phase modifier. Obtained RM0 values were correlated with lipophilicity, solubility, human intestinal absorption, plasma-protein binding, and blood,brain barrier data. The comparison among chromatographic data obtained by two mobile phase was performed with a statistical technique, principle component analysis. Copyright © 2009 John Wiley & Sons, Ltd. [source]