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Percent Recovery (percent + recovery)
Selected AbstractsEffect of Conductivity, pH, and Elution Buffer Salinity on Glycomacropeptide Recovery from Whey Using Anion Exchange ChromatographyJOURNAL OF FOOD SCIENCE, Issue 4 2005Hatice N. Tek ABSTRACT: The objective of this study was to investigate the effect of whey conductivity, pH, and the salt concentration of the elution buffer on glycomacropeptide recovery and its extent of contamination using anion exchange chromatography. Glycomacropeptide was isolated from Mozzarella whey. Samples were analyzed for glycomacropeptide and contaminating whey proteins. Mass balances and percent recoveries were calculated from these data. Glycomacropeptide recovery increased substantially with decreasing conductivity and increasing pH of the whey feed stream. Increasing the pH, but not increasing the conductivity, increased contamination of the glycomacropeptide by primarily beta-lactoglobulin. Salt concentration of at least 0.1 M was required for complete elution of bound glycomacropeptide. These data define conditions needed for glycomacropeptide recovery by a process chromatography system that uses food-grade buffers, operates at industrially relevant flow rates, and achieves up to 98% recovery. [source] Stability-indicating assay of sodium cromoglicate in ophthalmic solution using mixed-mode hydrophilic interaction chromatographyJOURNAL OF SEPARATION SCIENCE, JSS, Issue 9 2008Mohammed Shahid Ali Abstract A hydrophilic interaction chromatographic (HILIC) procedure for the quantification of Sodium Cromoglicate (SCG) in ophthalmic solution is developed. Mobile phase consists of ACN and buffer, 86:14 v/v. Atlantis HILIC,Si column, 25 cm×4.6 mm, is used as stationary phase. Detection is carried out using a variable wavelength UV-Vis detector at 326 nm. Linearity range and percent recoveries for SCG were 50,400 ,g/mL and 100.44%, respectively. The SCG HILIC-UV assay was validated according to the International Conference on Harmonization guidelines. The method separates two impurities and degradation products resulting from stress environment. Influence of organic solvent, ionic strength and mobile phase pH on the retention of SCG is studied. The paper provides optimization of polar anionic solute (SCG) on unmodified silica by HILIC. Proposed method can be used as a stability-indicating assay for SGC and can be proved to be beneficial in ESI-MS for enhanced sensitivity. [source] Conversion of Isoflavone Glycosides to Aglycones in SoyLife and Soymeal Using ,-glycosidaseJOURNAL OF FOOD SCIENCE, Issue 2 2003L. Xie ABSTRACT: Conversion of isoflavones from glycosides to aglycones in SoyLife and soymeal using varying concentrations of ,-glycosidase, and different pH conditions and temperatures was investigated. The best conditions for the conversion of glycosides to aglycones were pH 5.0, 50 °C, and 5 h incubation with 5 units ,-glycosidase/g Soy Life and 1.5 units/g soymeal. Under these conditions, the amount of genistein, daidzein, and glycitein in Soy Life treated with ,-glycosidase were 4.22, 11.52, and 11.85 ,mol/g compared to untreated controls of 0.26, 0.97, and 4.43 ,mol/g, respectively. In soymeal, the amounts were 3.21, 2.02, and 2.12 ,mol/g compared to untreated controls of 1.23, 1.25, and 1.51 ,mol/g, respectively. Mole percent recovery of genistein was 87% in Soy Life and 80% in soymeal, respectively. [source] Lactic acid quantitation in hand dishwashing liquid using an HILIC-UV methodologyJOURNAL OF SEPARATION SCIENCE, JSS, Issue 6-7 2010Mark Storton Abstract Different hydrophilic interaction chromatography (HILIC) columns were screened for lactic acid separation in hand dishwashing liquid products and the influence of mobile phase strength, buffer concentration and column temperature on the retention of lactic acid on a Zorbax NH2 column was investigated. An isocratic HILIC method for the quantitation of lactic acid in hand dishwashing liquid products was developed. The mobile phase consists of 70% methanol and 30% 20,mM sodium phosphate buffer (v/v) at pH 2.5. The HILIC stationary phase is Zorbax NH2, 250×4.6 with a 5,,m particle size. Detection was carried out using a variable wavelength UV-VIS detector at 226,nm. The linear range and percent recovery for lactic acid in the products were 44.68,1206.39,,g/mL and 100.3%, respectively. This paper provides an optimized HILIC methodology for the analysis of an acidic polar analyte (lactic acid) on a basic stationary phase. The proposed method can be used for the routine analysis of lactic acid. [source] Removal and Recovery of UO2(??) from Water Samples Using 2,2,-Diamino-4,4,-bithiazole as a New Reagent for Solid Phase ExtractionCHINESE JOURNAL OF CHEMISTRY, Issue 4 2010Majid Soleimani Abstract A novel sensitive and simple method for rapid and selective extraction, preconcentration and determination of uranyl as its 2,2,-diamino-4,4,-bithiazole (DABTZ) complex by using octadecylsilica columns and spectrophotometry is presented. Extraction efficiency and the influence of flow rates of sample solution and eluent, pH, amount of DABTZ, type and least amount of eluent for elution of uranyl complex from columns, break-through volume and limit of detection were evaluated. Also the effects of various cationic and anionic interferences on percent recovery of uranyl were studied. Average extraction efficiency of ca. 90% was obtained by elution of the column with minimal amount of solvent in the presence of interferences. The average preconcentration factor, 136 and a detection limit 0.32 ng·mL,1 were obtained. The method was applied to the recovery and determination of uranyl in different water samples. [source] | ||||||||||