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Part IV (part + iv)

Distribution by Scientific Domains
Humanities and Social Sciences11%

Selected Abstracts

Cover Picture: Electrophoresis 16'2010

ELECTROPHORESIS, Issue 17 2010
Article first published online: 7 SEP 2010
Issue no. 17 is a regular issue comprising 18 manuscripts distributed over 5 separate parts. Part I has 7 research articles on some aspects of proteins and cell separations. Part II has 3 research articles on nucleic acid research including cloning/amplification, gene regulation and STR analysis. Part III offers ways of measuring diffusion and binding constants in two separate articles. Concentration and detection approaches are treated in 4 research articles making up Part IV. The last two articles in this issue (Part V) are on CEC and EKC describing a mixed mode monolithic stationary phase and a cyclodextrin-modified MEEKC. Featured articles include: Lamp-based wavelength-resolved fluorescence detection for protein capillary electrophoresis: Set-up and detector performance ((doi: 10.1002/elps.201000246)) Electromigration diffusivity spectrometry: A way for simultaneous determination of diffusion coefficients from mixed samples ((doi: 10.1002/elps.201000252)) Sample stacking capillary electrophoretic microdevice for highly sensitive mini Y short tandem repeat genotyping ((doi: 10.1002/elps.201000270)) [source]

Cover Picture: Electrophoresis 16'2010

ELECTROPHORESIS, Issue 16 2010
Article first published online: 19 AUG 2010
Issue no. 16 is a regular issue with an Emphasis on "Proteins and Proteomics" comprising 20 manuscripts distributed over 4 separate parts. Part I has 7 research articles on various aspects of proteins and proteomics including combinatorial peptide ligand library for accessing low abundance proteins, analysis of membrane proteins, proteomic profiling of human colon cancer cells, quantitative determinations of biomarkers in clinical diagnostics, recombinant factor VIII, analysis of E. coli soluble proteins, and a weakly basic amino-reactive fluorescent label for IEF of proteins and chip electrophoresis. Part II has 2 research articles dealing with the CE analysis of magnetic nanoparticles and a microfluidic magnetic bead impact for cell stimulation. Part III consists of 2 research articles dealing with on-line preconcentration in CE. Instrumentation, devices and various methodologies are described in 9 research articles, which make the content of Part IV. Featured articles include: Combinatorial peptide ligand library plasma treatment: Advantages for accessing low-abundance proteins ((doi: 10.1002/elps.201000188)) Precautions to improve the accuracy of quantitative determinations of biomarkers in clinical diagnostics ((doi: 10.1002/elps.201000243)) Rapid identification of Candida albicans in blood by combined capillary electrophoresis and fluorescence in situ hybridization ((doi: 10.1002/elps.201000138)) [source]

Cover Picture: Electrophoresis 14'2010

ELECTROPHORESIS, Issue 14 2010
Article first published online: 21 JUL 2010
Issue no. 14 is a "mini special issue" on "Microscale Separation Methods for Metabolomics" comprising 9 manuscripts on metabolomics and 12 manuscripts on various topics in nucleic acids, biomarkers, proteomics, miniaturization, etc. Part I has 9 manuscripts on metabolomics featuring new technological developments and the potential of CE-MS, targeted analysis of one class of metabolites and non-targeted analysis, and data interpretation that is essential to acquire useful biological information. In short, the importance of CE and, generally, of microscale separation methods for metabolomics is rapidly increasing and the papers published in this issue give an overview of this field. Part II has 2 research papers on biomarkers while Part III is on various aspects of nucleic acids including but not limited to genotyping, PCR, SSCP, PCR and detection of DNA. Part IV describes various aspects of fundamentals and methodology in microfluidics, cell lysates by 2-DE, CE-LIF of plasmid DNA, whole blood assay of trypsin activity, etc. [source]

Cover Picture: Electrophoresis 13'2010

ELECTROPHORESIS, Issue 13 2010
Article first published online: 30 JUN 2010
Issue no. 13 is a special issue on "Food and Beverage Analysis" comprising 23 contributions distributed over four distinct parts. Part I "presents six review papers, five of them are focused on the use of capillary electromigration techniques for detecting enantiomers, nucleosides, nucleotides, pesticides and contaminants in foods as well as foodborne pathogens". The sixth review deals with the application of two-dimensional electrophoresis to investigate food allergens. A series of original contributions on the use of CE, CEC and MEKC in the analysis of food constituents are grouped in Part II. Part III presents "Works on the detection of genetically modified foods by CGE and the analysis of antibiotics and antioxidants by microemulsion electrokinetic chromatography". Finally, Part IV is on the use of CE-MS to analyze endocrine disruptors and polyphenols in different food matrices, and on the speciation analysis of arsenic and selenium compounds in different foodstuffs by capillary electrophoresis-inductively coupled plasma-mass spectrometry. [source]

Cover Picture: Electrophoresis 12'2010

ELECTROPHORESIS, Issue 12 2010
Article first published online: 18 JUN 2010
Issue no. 12 is a regular issue comprising 19 contributions distributed over five distinct parts. Part I has 7 articles describing novel methodologies pertaining to proteins and proteomics. Part II has 3 research papers on CEC stationary phases and CEC-MS. Part III is on detection approaches including a review article on the advances and applications of chemiluminescence coupled to CE. Part IV has two papers on enantioseparations, and Part V has four contributions on aptamers, human genetic, preparative FFE and microchannels. Featured articles include: Simplified method for concentration of mitochondrial membrane protein complexes ((10.1002/elps.201000019)) Analysis of low-molecular mass aldehydes in drinking waters through capillary electrophoresis with laser-induced fluorescence detection ((10.1002/elps.200900734)) Selection of aptamers for signal transduction proteins by capillary electrophoresis ((10.1002/elps.200900543)) [source]

Cover Picture: Electrophoresis 11'2010

ELECTROPHORESIS, Issue 11 2010
Article first published online: 26 MAY 2010
Issue no. 11 is a special issue on "Bioanalysis" comprising 18 manuscripts distributed over six distinct parts. Part I has 3 review articles describing a novel approach for biopolymer focusing, free flow electrophoresis and mass spectrometry analysis of deamidation of proteins. Part II has 2 research papers on some fundamentals and methodology. Part III is on microRNA while Part IV is on protein and protein complex analysis using CE and microfluidics. Part V, which is on gel based protein analysis and proteomic profiling, has 7 contributions making the bulk of the special issue. Finally, Part VI is on metabolic and toxicological profiling and has 3 contributions. [source]

Cover Picture: Electrophoresis 10'2010

ELECTROPHORESIS, Issue 10 2010
Article first published online: 18 MAY 2010
Issue no. 10 is a regular issue comprising 19 manuscripts distributed over four distinct parts. Part I is on microfluidics and miniaturized systems and has 5 articles; Part II is on nucleic acids with 4 articles on restriction endonuclease fingerprinting, mutation detection and DNA separation and detection; Part III has 7 articles on monolithic stationary phases for CEC, single walled carbon nanohorns as pseudo-stationary phases for CEC and EKC, MEEKC, cyclodextrin-modified gold nanoparticles for enantioseparations by CEC, use of divalent dipeptides as counter ions in CE and capillary coating for CE of proteins; and Part IV has 3 articles on proteomics methodologies. Featured articles include: Microfluidic preparative free-flow isoelectric focusing in a triangular channel: System development and characterization ((10.1002/elps.200900577)) Separation and recovery of nucleic acids with improved biological activity by acid-degradable polyacrylamide gel electrophoresis ((10.1002/elps.200900783)) Evaluation of the performance of single-walled carbon nanohorns in capillary electrophoresis ((10.1002/elps.200900628)) The inter- and intra-operator variability in manual spot segmentation and its effect on spot quantitation in two dimensional electrophoresis analysis. ((10.1002/elps.200900674)) [source]

Cover Picture: Electrophoresis 8'2010

ELECTROPHORESIS, Issue 8 2010
Article first published online: 20 APR 2010
Issue no. 8 is a regular issue comprising19 manuscripts distributed over four distinct parts. Part I is on proteins and proteomics and has 5 articles; Part II is on nucleic acids with 5 articles on DNA purification, sequencing, genotyping and differential gene expression; Part III has 4 articles on droplet dispensing and particle separation; Part IV is on various methodologies and applications assembling 5 articles on improved sample preparation method for glycan analysis by CE, measurement of intracellular accumulation chemotherapeutic drugs in cancerous cells, metabolic monitoring in microfluidic cell arrays, microchip electrophoresis for continuous monitoring of microdialysis samples, and determination of glyphosate and its metabolites in plant materials by CE. Featured articles include: Delta2D and Proteomweaver: Performance evaluation of two different approaches for two-dimensional electrophoresis analysis. ((10.1002/elps.200900766)) A Multidimensional Electrophoretic System of Separation for the Analysis of Gene Expression (MESSAGE). ((10.1002/elps.200900624)) Particle trapping using dielectrophoretically patterned carbon nanotubes. ((10.1002/elps.200900717)) [source]

Cover Picture: Electrophoresis 6'2010

ELECTROPHORESIS, Issue 6 2010
Article first published online: 22 MAR 2010
Issue no. 6 is a regular issue consisting of 18 full research papers. The first paper is a "Fast Track" contribution involving microchip electrophoresis of Alu elements for gender determination and inference of human ethnic origin. The remaining 17 papers are distributed over 5 different parts: Part I is on some trends in CEC; Part II deals with enantioseparations; Part III describes some novel detection approaches; Part IV has investigations on proteomics and proteins; Part V tackles the subjects related to pathogens, bacteria and the binding of aptamers to small solutes. [source]

Cover Picture: Electrophoresis 5'2010

ELECTROPHORESIS, Issue 5 2010
Article first published online: 26 FEB 2010
Issue no. 5 is a Special issue on "Fundamentals of Electrophoresis". Part I has 5 review articles on the various fields of electrophoresis. Separation in nano-channels is treated in 3 articles in Part II. Part III has 3 research papers on computer simulation of electromigration and Part IV also has 3 articles on stacking and focusing. Interaction in electrophoretic systems is the subject of Part V which has 6 research papers. Finally, electroosmotic flow is described in 1 paper in part VI. [source]

Cover Picture: Electrophoresis 22'2009

ELECTROPHORESIS, Issue 22 2009
Article first published online: 25 NOV 200
Issue no. 22 is a Special Issue on "CE and CEC Innovations" consisting of 24 important contributions in various areas of CE and CEC that are grouped into five different parts. Part I has 7 articles on novel "Stationary Phases for CEC". Part II is on "CE of Microorganisms and their Components and Interactions", and has 4 research articles. "Enantioseparations" constitute part III and has 3 research articles dealing with different chiral species and chiral CE systems. Part IV has 3 contributions on "Detection Approaches in CE". Part V is on "Capillary Coating, Affinity and Separation Media , Applications" and contains 7 research articles dealing with the separations of proteins, lipoproteins, bioactive inflammatory cytokines, inorganic and small organic anions, non-steroidal anti-inflammatory drugs, cell culture media and ancient DNA samples." [source]

Cover Picture: Electrophoresis 4'09

ELECTROPHORESIS, Issue 4 2009
Article first published online: 26 FEB 200
Issue no. 4 is a special issue on "CEC and EKC" containing 20 papers including 2 Fast Track papers. The first Fast Track paper deals with high-resolution computer simulations of EKC while the second Fast Track paper reports the use of camera cell phone to detect the gold nanoparticle-labeled immunoassay results on microfluidic chip. The remaining 18 papers of this special issue are distributed into four parts. Part I is on monolithic capillaries for CEC and has five papers demonstrating the continuous efforts in developing novel monolithic stationary phases. Part II, which assembles six contributions, is on various aspects of MEKC separations of a wide range of compounds in the presence of different types of micelles. On-line concentration cum EKC/CEC is the subject of Part III that has five contributions demonstrating signal enhancement for various small and large molecules. This issue concludes with Part IV on enantioseparations by chiral EKC and CEC. [source]

Teaching and Learning Guide for: Memoryscape: How Audio Walks Can Deepen Our Sense of Place by Integrating Art, Oral History and Cultural Geography

GEOGRAPHY COMPASS (ELECTRONIC), Issue 5 2008
Toby Butler
Author's Introduction This article is concerned with the history and practice of creating sound walks or ,memoryscapes': outdoor trails that use recorded sound and spoken memory played on a personal stereo or mobile media to experience places in new ways. It is now possible to cheaply and easily create this and other kinds of located media experience. The development of multi-sensory-located media (,locedia') presents some exciting opportunities for those concerned with place, local history, cultural geography and oral history. This article uses work from several different disciplines (music, sound art, oral history and cultural geography) as a starting point to exploring some early and recent examples of locedia practice. It also suggests how it might give us a more sophisticated, real, embodied and nuanced experience of places that the written word just can not deliver. Yet, there are considerable challenges in producing and experiencing such work. Academics used to writing must learn to work in sound and view or image; they must navigate difficult issues of privacy, consider the power relations of the outsider's ,gaze' and make decisions about the representation of places in work that local people may try and have strong feelings about. Creating such work is an active, multi-sensory and profoundly challenging experience that can offer students the chance to master multi-media skills as well as apply theoretical understandings of the histories and geographies of place. Author Recommends 1.,Perks, R., and Thomson, A. (2006). The oral history reader, 2nd ed. London: Routledge. This is a wonderful collection of significant writing concerned with oral history. Part IV, Making Histories features much of interest, including a thought-provoking paper on the challenges of authoring in sound rather than print by Charles Hardy III, and a moving interview with Graeme Miller, the artist who created the Linked walk mentioned in the memoryscape article. These only feature in the second edition. 2.,Cresswell, T. (2004). Place: a short introduction. Oxford, UK: Blackwell. A refreshingly clear and well-written guide to the different theoretical takes on what makes places , a good starting point for further reading. 3.,Carlyle, A. (ed.). (2008). Autumn leaves: sound and the environment in artistic practice. Paris, France: Double Entendre. This is a collection of short essays and examples of located sonic media art; it includes interviews with practitioners and includes Hildegard Westekamp's Soundwalking, a practical guide to leading students on a mute walk. Lots of thought provoking, applied reading material for students here. 4.,Blunt, A., et al. (eds) (2003). Cultural geography in practice. London: Arnold. A great book for undergraduate and postgraduate students , concepts explained and lots of examples of actually doing cultural geography. The chapter on mapping worlds by David Pinder is particularly useful in this context. 5.,Pinder, D. (2001). Ghostly footsteps: voices, memories and walks in the city. Ecumene 8 (1), pp. 1,19. This article is a thoughtful analysis of a Janet Cardiff sound walk in Whitechapel, East London. Online Materials http://www.memoryscape.org.uk This is my project website, which features two online trails, Dockers which explores Greenwich and the memories of the London Docks that are archived in the Museum of London, and Drifting which is a rather strange experiment-combining physical geography and oral history along the Thames at Hampton Court, but still makes for an interesting trail. Audio, maps and trails can be downloaded for free, so students with phones or iPods can try the trails if you are within reach of Surrey or London. The site features an online version, with sound-accompanying photographs of the location. http://www.portsofcall.org.uk This website has three more trails here, this time of the communities surrounding the Royal Docks in East London. The scenery here is very dramatic and anyone interested in the regeneration of East London and its impact on local communities will find these trails interesting. Like Dockers, the walks feature a lot of rare archive interviews. This project involved a great deal of community interaction and participation as I experimented with trying to get people involved with the trail-making process. The site uses Google maps for online delivery. http://www.soundwalk.com This New York-based firm creates exceptionally high-quality soundwalks, and they are well worth the money. They started by producing trails for different districts of New York (I recommend the Bronx Graffiti trail) and have recently made trails for other cities, like Paris and Varanassi in India. http://www.mscapers.com This website is run by Hewlett Packard, which has a long history of research and development in located media applications. They currently give free licence to use their mscape software which is a relatively easy to learn way of creating global positioning system-triggered content. The big problem is that you have to have a pricey phone or personal digital assistant to run the software, which makes group work prohibitively expensive. But equipment prices are coming down and with the new generations of mobile phones developers believe that the time when the player technology is ubiquitous might be near. And if you ask nicely HP will lend out sets of equipment for teaching or events , fantastic if you are working within reach of Bristol. See also http://www.createascape.org.uk/ which has advice and examples of how mscape software has been used for teaching children. Sample Syllabus public geography: making memoryscapes This course unit could be adapted to different disciplines, or offered as a multidisciplinary unit to students from different disciplines. It gives students a grounding in several multi-media techniques and may require support/tuition from technical staff. 1.,Introduction What is a located mediascape, now and in the future? Use examples from resources above. 2.,Cultural geographies of site-specific art and sound Theories of place; experiments in mapping and site-specific performance. 3.,Walk activity: Westergard Hildekamp , sound walk, or one of the trails mentioned above The best way , and perhaps the only way , to really appreciate located media is to try one in the location they have been designed to be experienced. I would strongly advise any teaching in this field to include outdoor, on-site experiences. Even if you are out of reach of a mediascape experience, taking students on a sound walk can happen anywhere. See Autumn Leaves reference above. 4.,Researching local history An introduction to discovering historical information about places could be held at a local archive and a talk given by the archivist. 5.,Creating located multimedia using Google maps/Google earth A practical exercise-based session going through the basics of navigating Google maps, creating points and routes, and how to link pictures and sound files. 6.,Recording sound and oral history interviews A practical introduction to the techniques of qualitative interviewing and sound recording. There are lots of useful online guides to oral history recording, for example, an online oral history primer http://www.nebraskahistory.org/lib-arch/research/audiovis/oral_history/index.htm; a more in depth guide to various aspects of oral history http://www.baylor.edu/oral%5fhistory/index.php?id=23566 or this simple oral history toolkit, with useful links to project in the North of England http://www.oralhistorynortheast.info/toolkit/chapter1.htm 7.,Sound editing skills Practical editing techniques including working with clips, editing sound and creating multi-track recordings. The freeware software Audacity is simple to use and there are a lot of online tutorials that cover the basics, for example, http://www.wikieducator.org/user:brentsimpson/collections/audacity_workshop 8.,Web page design and Google maps How to create a basic web page (placing pictures, text, hyperlinks, buttons) using design software (e.g. Dreamweaver). How to embed a Google map and add information points and routes. There is a great deal of online tutorials for web design, specific to the software you wish to use and Google maps can be used and embedded on websites free for non-profit use. http://maps.google.com/ 9,and 10. Individual or group project work (staff available for technical support) 11.,Presentations/reflection on practice Focus Questions 1What can sound tell us about the geographies of places? 2When you walk through a landscape, what traces of the past can be sensed? Now think about which elements of the past have been obliterated? Whose past has been silenced? Why? How could it be put back? 3Think of a personal or family story that is significant to you. In your imagination, locate the memory at a specific place. Tell a fellow student that story, and describe that place. Does it matter where it happened? How has thinking about that place made you feel? 4What happens when you present a memory of the past or a located vision of the future in a present landscape? How is this different to, say, writing about it in a book? 5Consider the area of this campus, or the streets immediately surrounding this building. Imagine this place in one of the following periods (each group picks one): ,,10,000 years ago ,,500 years ago ,,100 years ago ,,40 years ago ,,last Thursday ,,50 years time What sounds, voices, stories or images could help convey your interpretation of this place at that time? What would the visitor hear or see today at different points on a trail? Sketch out an outline map of a located media trail, and annotate with what you hear/see/sense at different places. Project Idea small group project: creating a located mediascape Each small group must create a located media experience, reflecting an aspect of the history/geography/culture of an area of their choosing, using the knowledge that they have acquired over the course of the semester. The experience may be as creative and imaginative as you wish, and may explore the past, present or future , or elements of each. Each group must: ,,identify an area of interest ,,research an aspect of the area of the groups choosing; this may involve visiting local archives, libraries, discussing the idea with local people, physically exploring the area ,,take photographs, video or decide on imagery (if necessary) ,,record sound, conduct interviews or script and record narration ,,design a route or matrix of media points The final project must be presented on a website, may embed Google maps, and a presentation created to allow the class to experience the mediascape (either in the classroom or on location, if convenient). The website should include a brief theoretical and methodological explanation of the basis of their interpretation. If the group cannot be supported with tuition and support in basic website design or using Google mapping with sound and imagery, a paper map with locations and a CD containing sound files/images might be submitted instead. For examples of web projects created by masters degree students of cultural geography at Royal Holloway (not all sound based) see http://www.gg.rhul.ac.uk/MA/web-projects.html [source]

Development and validation of the Unified Multiple System Atrophy Rating Scale (UMSARS)

MOVEMENT DISORDERS, Issue 12 2004
Gregor K. Wenning MD
Abstract We aimed to develop and validate a novel rating scale for multiple system atrophy (Unified Multiple System Atrophy Rating Scale - UMSARS). The scale comprises the following components: Part I, historical, 12 items; Part II, motor examination, 14 items; Part III, autonomic examination; and Part IV, global disability scale. For validation purposes, 40 MSA patients were assessed in four centers by 4 raters per center (2 senior and 2 junior raters). The raters applied the UMSARS, as well as a range of other scales, including the Unified Parkinson's Disease Rating Scale (UPDRS) and the International Cooperative Ataxia Rating Scale (ICARS). Internal consistency was high for both UMSARS-I (Crohnbach's alpha = 0.84) and UMSARS-II (Crohnbach's alpha = 0.90) sections. The interrater reliability of most of the UMSARS-I and -II items as well as of total UMSARS-I and -II subscores was substantial (k (w) = 0.6,0.8) to excellent (k (w) > 0.8). UMSARS-II correlated well with UPDRS-III and ICARS (rs > 0.8). Depending on the degree of the patient's disability, completion of the entire UMSARS took 30 to 45 minutes. Based on our findings, the UMSARS appears to be a multidimensional, reliable, and valid scale for semiquantitative clinical assessments of MSA patients. © 2004 Movement Disorder Society [source]

Mexico: New Democracy with Old Parties?,

POLITICS, Issue 3 2003
Carlos E. Casillas
Mexico's 2000 presidential election was one of the most important political events in the nation's contemporary history. The victory of the National Action Party (PAN) and Vicente Fox, the first ,non-official' candidate ever to win a Mexican presidential election, surprised both local and world observers. This article comprises four parts. Part I very briefly places the election in historical perspective. In Part II, each of the three front-runners in the contest is profiled. Part III includes a systematic analysis of the general election results by constituencies or other territorial units, and features tabulated data. Part IV addresses the development of political parties and the party system before and after the elections. [source]

Cover Picture: Electrophoresis 19'2010

ELECTROPHORESIS, Issue 19 2010
Article first published online: 29 SEP 2010
Issue no. 19 is a special issue on "CE and CEC Innovations" comprising 1 Fast Track manuscript and 20 manuscripts distributed over 5 separate parts. Part I has 5 research articles on novel trends in CEC. Part II has 4 research articles dealing with innovative methodologies. Part III reports a variety of interactive CE systems described in 5 research articles. Proteins and biomarkers are treated in 3 research articles making up part IV. The last 3 articles in this issue (Part V) are on detection approaches. The FAST TRACK article describes "An automated capillary electrophoresis system for high speed separation of DNA fragments based on a short capillary". [source]

Cover Picture: Electrophoresis 18'2010

ELECTROPHORESIS, Issue 18 2010
Article first published online: 20 SEP 2010
Issue no. 18 is a special issue on "Miniaturization in Asia-Pacific" comprising 21 manuscripts distributed over 5 separate parts. Part I has 1 review article on microfluidics gradient platform for controlling cellular behavior. Part II has 6 research articles dealing with various basic technologies in microfluidics. Part III reports a variety of detection approaches described in 6 research articles. DNA and protein analysis using microfluidics are treated in 3 research articles making up part IV. The last 5 articles in this issue (Part V) are on cell analysis. [source]