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Palmitic Acid (palmitic + acid)
Selected AbstractsFatty acid composition in some selected marine fish species living in Turkish watersJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 1 2006Abdulkadir Bay Abstract The muscle fatty acid (FA) composition of 12 selected marine fish species living in Turkish waters was determined. FA profiles of muscle were compared in terms of total and individual saturated (SFAs) and unsaturated fatty acids. Total SFA, monounsaturated fatty acid (MUFA), n-3 polyunsaturated fatty acid (n-3 PUFA) and n-6 polyunsaturated fatty acid (n-6 PUFA) contents as well as n-3/n-6 FA and eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA) ratios differed (P < 0.01) in all fish species. Palmitic acid (16:0) among total SFAs, oleic acid (18:1n-9) among MUFAs, DHA (22:6n-3) among n-3 PUFAs and 18:2n-6 and 20:4n-6 among n-6 PUFAs were the most abundant FAs. The ratio of n-3/n-6 PUFA contents, indicating the availability of n-3 PUFAs that are beneficial for human health, ranged from 2.67 to 12.61. The EPA/DHA ratio was between 0.17 and 0.83 in the marine fish species studied. Copyright © 2005 Society of Chemical Industry [source] Short-term administration of (-)-epigallocatechin gallate reduces hepatic steatosis and protects against warm hepatic ischemia/reperfusion injury in steatotic miceLIVER TRANSPLANTATION, Issue 3 2005Ryan N. Fiorini Hepatic steatosis increases the extent of cellular injury incurred during ischemia/reperfusion (I/R) injury. (-)-Epigallocatechin gallate (EGCG), the major flavonoid component of green tea (camellia sinensis) is a potent antioxidant that inhibits fatty acid synthase (FAS) in vitro. We investigated the effects of EGCG on hepatic steatosis and markers of cellular damage at baseline and after I/R injury in ob/ob mice. Animals were pretreated with 85 mg/kg EGCG via intraperitoneal (ip) injection for 2 days or oral consumption in the drinking water for 5 days before 15 minutes of warm ischemia and 24 hours of reperfusion. After EGCG administration, total baseline hepatic fat content decreased from baseline. Palmitic acid and linoleic acid levels also were reduced substantially in all ECGC-treated animals before I/R. Alanine aminotransferase (ALT) levels decreased in all EGCG-treated animals compared with control animals after I/R. Histologic analysis demonstrated an average decrease of 65% necrosis after EGCG administration. EGCG administration also increased resting hepatic energy stores as determined by an increase in cellular adenosine triphosphate (ATP) with a concomitant decrease in uncoupling protein 2 (UCP2) before I/R. Finally, there was an increased level of glutathione (GSH) in the EGCG-treated mice compared with the vehicle-treated mice both at baseline and after I/R. In conclusion, taken together, this study demonstrates that treatment with ECGC by either oral or ip administration, significantly protects the liver after I/R, possibly by reducing hepatic fat content, increasing hepatic energy status, and functioning as an antioxidant. (Liver Transpl 2005;11:298,308.) [source] Fatty acid oxidation and meiotic resumption in mouse oocytesMOLECULAR REPRODUCTION & DEVELOPMENT, Issue 9 2009Stephen M. Downs We have examined the potential role of fatty acid oxidation (FAO) in AMP-activated protein kinase (AMPK)-induced meiotic maturation. Etomoxir and malonyl CoA, two inhibitors of carnitine palmitoyl transferase-1 (CPT1), and thus FAO, blocked meiotic induction in dbcAMP-arrested cumulus cell-enclosed oocytes (CEO) and denuded oocytes (DO) by the AMPK activator, AICAR. C75, an activator of CPT1 and FAO, stimulated meiotic resumption in CEO and DO. This effect was insensitive to the AMPK inhibitor, compound C, indicating an action downstream of AMPK. Palmitic acid or carnitine also promoted meiotic resumption in DO in the presence of AICAR. Since C75 also suppresses the activity of fatty acid synthase (FAS), we tested another FAS inhibitor, cerulenin. Cerulenin stimulated maturation in arrested oocytes, but to a lesser extent, exhibited significantly slower kinetics and was effective in CEO but not DO. Moreover, etomoxir completely blocked C75-induced maturation but was ineffective in cerulenin-treated oocytes, suggesting that the meiosis-inducing action of C75 is through activation of FAO within the oocyte, while that of cerulenin is independent of FAO and acts within the cumulus cells. Finally, we determined that long chain, but not short chain, fatty acyl carnitine derivatives were stimulatory to oocyte maturation. Palmitoyl carnitine stimulated maturation in both CEO and DO, with rapid kinetics in DO; this effect was blocked by mercaptoacetate, a downstream inhibitor of FAO. These results indicate that activation of AMPK stimulates meiotic resumption in mouse oocytes by eliminating a block to FAO. Mol. Reprod. Dev. 76: 844,853, 2009. © 2009 Wiley-Liss, Inc. [source] Decrease in stearic acid proportions in adipose tissues and liver lipids in fatty liver of dairy cowsANIMAL SCIENCE JOURNAL, Issue 3 2006Hiroshi SATO ABSTRACT Samples of liver and perirenal, mesenteric and subcutaneous fat were collected from 16 sick necropsied dairy cows to evaluate the fatty acid profiles in the hepatic and adipose tissues associated with advanced fatty liver or hepatic lipidosis. Hepatic triglyceride and eight fatty acids were measured in the hepatic and adipose tissues. Six cows had more than 3% triglyceride on fresh weight in their livers and were classified as having fatty liver. Stearic and linoleic acid proportions in the liver decreased markedly with increased hepatic triglyceride levels, while the proportion of palmitic and oleic acids increased. The most striking fluctuations in hepatic lipidosis were manifested as decreased stearic acid in the adipose tissues including subcutaneous fat with the trend of decreasing stearic acid. Palmitic acid was elevated in hepatic and perirenal fat in fatty liver cows. In instances of advanced hepatic lipidosis, palmitoleic acid increased in only subcutaneous fat and not in perirenal or mesenteric fat. In addition to the proportions of hepatic fatty acids in fatty liver, this study also clarified the fluctuations observed in the profiles of fatty acids of the adipose tissues in cows with advanced hepatic lipidosis, particularly the decline in the proportions of stearic acid. [source] Influence of dietary palm oil on growth, tissue fatty acid compositions, and fatty acid metabolism in liver and intestine in rainbow trout (Oncorhynchus mykiss)AQUACULTURE NUTRITION, Issue 4 2005J. FONSECA-MADRIGAL Abstract This study aimed to investigate the effects of dietary crude palm oil (CPO) on fatty acid metabolism in liver and intestine of rainbow trout. Triplicate groups of rainbow trout for 10 weeks at 13 °C were fed on diets in which CPO replaced fish oil (FO) in a graded manner (0,100%). At the end of the trial, fatty acid compositions of flesh, liver and pyloric caeca were determined and highly unsaturated fatty acid (HUFA) synthesis and fatty acid oxidation were estimated in isolated hepatocytes and caecal enterocytes using [1- 14C]18:3n-3 as substrate. Growth performance and feed efficiency were unaffected by dietary CPO. Fatty acid compositions of selected tissues reflected the dietary fatty acid composition with increasing CPO resulting in increased proportions of 18:1n-9 and 18:2n-6 and decreased proportions of n-3HUFA, 20:5n-3 and 22:6n-3. Palmitic acid, 16:0, was also increased in flesh and pyloric caeca, but not in liver. The capacity of HUFA synthesis from 18:3n-3 increased by up to threefold in both hepatocytes and enterocytes in response to graded increases in dietary CPO. In contrast, oxidation of 18:3n-3 was unaffected by dietary CPO in hepatocytes and reduced by high levels of dietary CPO in enterocytes. The results of this study suggest that CPO can be used at least to partially replace FO in diets for rainbow trout in terms of permitting similar growth and feed conversion, and having no major detrimental effects on lipid and fatty acid metabolism, although flesh fatty acid compositions are significantly affected at an inclusion level above 50%, with n-3HUFA reduced by up to 40%. [source] Eicosanoids influence in vitro elongation of plasmatocytes from the tobacco hornworm, Manduca sextaARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY (ELECTRONIC), Issue 1 2005Jon S. Miller Abstract Nodule formation is the predominant insect cellular defense reaction to bacterial challenges, responsible for clearing the largest proportion of infecting bacteria from hemolymph circulation. Hemocyte spreading behavior is a critical step in the nodulation process. It has been suggested that eicosanoids mediate several steps in the process. However, the influence of eicosanoids on hemocyte spreading has not been investigated in detail. To test the hypothesis that eicosanoids mediate hemocyte spreading behavior, I treated larvae of the tobacco hornworm, Manduca sexta, with eicosanoid biosynthesis inhibitors and later assessed plasmatocyte elongation on glass slides. Plasmatocytes from larvae treated with dexamethasone did not elongate to the extent of plasmatocytes from untreated control larvae. The dexamethasone effect on plasmatocyte elongation was expressed in a dose-dependent manner and was reversed by injecting dexamethasone-treated larvae with the eicosanoid-precursor fatty acid, arachidonic acid. Palmitic acid, which is not substrate for eicosanoid biosynthesis, did not reverse the influence of dexamethasone on plasmatocyte elongation. Finally, plasmatocytes from larvae treated with a range of eicosanoid biosynthesis inhibitors did not elongate to the extent of plasmatocytes from control larvae. Plasmatocyte width did not appear to be influenced in this study. These findings strongly support the idea that insect plasmatocyte elongation is influenced by eicosanoids. Arch. Insect Biochem. Physiol. 59:42,51, 2005. © 2005 Wiley-Liss, Inc. [source] Fatty acid incorporation in endothelial cells and effects on endothelial nitric oxide synthaseEUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Issue 9 2007S. Couloubaly Abstract Background The nature of fatty acids provided by the diet as well as plasma lipid metabolism can modify the composition and properties of plasma membrane and thus the activity of membrane proteins. In humans, as well as in experimental models, diabetes is associated with both an alteration in serum lipid profile and a documented endothelial dysfunction. This in vitro study investigated on an immortalized human endothelial cell line (EA.hy 926) the specific effects of several free fatty acids (FFAs) on the composition of cellular membranes and the regulation of endothelial nitric oxide synthase (eNOS). Materials and methods 0·1% of lipid deprived serum was added to the incubation medium with 25 mm glucose in order to study the effects of individual fatty acids: myristic acid, palmitic acid, stearic acid, oleic acid or linoleic acid at 100 µm bound with albumin. The effects of the FFAs on the endothelial nitric oxide synthase were investigated on mRNA level by quantitative PCR, on protein level and Ser1177 phosphorylation by Western blot and on enzymatic activity on living cells using radiolabelled arginine. Results Free linoleic acid increased the membrane content in n-6 fatty acids (mainly C18: n-6 and its metabolites) with a decrease in saturated and monounsaturated fatty acids. These conditions decreased the basal eNOS activity and reduced the phosphorylation of eNOS-Ser1177 due to activation by histamine. Free palmitic acid enriched the membranes with 16 : 0 with a slight decrease in monounsaturated fatty acids. These conditions increased eNOS activation without increasing Ser1177 phosphorylation upon histamine activation. The addition of the other FFAs also resulted in modifications of membrane composition, which did not to affect eNOS-Ser1177 phosphorylation. Conclusion Among the fatty acids used, only modification of the membrane composition due to linoleic acid supply disturbed the basal enzymatic activity and Ser1177 phosphorylation of eNOS in a way that limited the role of histamine activation. Linoleic acid might involve the dysfunction of both eNOS basal activity and its phosphorylation status and may then contribute to an impaired vasodilatation in vivo. [source] Mechanism of modulation of T cell responses by N-palmitoylated peptidesEUROPEAN JOURNAL OF IMMUNOLOGY, Issue 12 2004Clara Bueno Abstract Small structural changes in the antigenic peptides recognized by TCR can alter the biological properties of those peptides and convert them into weak agonists, partial agonists, or antagonists of these receptors. These altered peptide ligands (APL) are usually generated by conservative amino acid substitutions at TCR contact residues. Here, we show that APL with therapeutic properties can also be generated by attachment of palmitic acid at the N terminus of the peptide without the need to modify the peptide's primary sequence. Using N-palmitoylated pigeon cytochrome-c peptide 81,104 (PALPCC81,104), we were able to induce T cell hyporesponsiveness to the wild-type peptide in vitro. More importantly, administration of the PALPCC81,104 to mice reduced the responsiveness to the native peptide when tested ex vivo. Biochemical and functional experiments indicated that the action of N-palmitoylated peptides was due to the conversion of the native peptide into a weak agonist that could then induce T cell anergy. Our results demonstrate that N-palmitoylation of antigenic peptides is a feasible strategy to generate APL, as it avoids the need to screen multiple amino acid variants of each specific antigen to identify those with therapeutic properties. [source] Chemical evaluation of some paprika (Capsicum annuum L.) seed oilsEUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, Issue 12 2009Bertrand Matthäus Dr. Abstract The oil contents of seeds from paprika (Capsicum annuum L.) collected from different locations in Turkey and Italy varied in a relatively wide range from 8.5,g/100,g to 32.6,g/100,g. The fatty acid, tocopherol and sterol contents of the oils from different paprika seeds were investigated. The main fatty acids in paprika seed oils were linoleic acid (69.5,74.7,g/100,g), oleic acid (8.9,12.5,g/100,g) and palmitic acid (10.7,14.2,g/100,g). The oils contained an appreciable amount of ,-tocopherol (306.6,602.6,mg/kg), followed by ,-tocopherol (7.3,148.7,mg/kg). The major sterols were ,-sitosterol (1571.4,4061.7,mg/kg), campesterol (490.8,1182.7,mg/kg), and ,5 -avenasterol (374.5,899.6,mg/kg). The total concentration of sterols ranged from 3134.0,mg/kg to 7233.7,mg/kg. Remarkable amounts of cholesterol were found in the different samples (164.6,491.0,mg/kg). The present study showed that paprika seeds are a potential source of valuable oil that could be used for edible and industrial applications. [source] Thermostability of genetically modified sunflower oils differing in fatty acid and tocopherol compositionsEUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, Issue 8 2008Susana Marmesat Abstract The objective of the study was to investigate the performance at frying temperature of a new sunflower oil with high content of oleic and palmitic acid (HOHPSO) and containing ,-tocopherol as the most abundant natural antioxidant. HOHPSO either containing ,- or ,-tocopherol (HOHPSO-, and HOHPSO-,, respectively) were obtained from genetically modified sunflower seeds and refined under identical conditions. The oil stability against oxidation, as measured by Rancimat at 120,°C, was much higher for the oil containing ,-tocopherol, suggesting the higher effectiveness of ,-tocopherol as compared to ,-tocopherol to delay oxidation. Experiments at high temperature (180,°C) simulating the conditions applied in the frying process clearly demonstrated that, for the same periods of heating, the oil degradation and the loss of natural tocopherol were significantly lower for the oil containing ,-tocopherol. Comparison of different genetically modified sunflower oils with different fatty acid compositions confirmed that oil degradation depended on the fatty acid composition, being higher at a higher degree of unsaturation. However, the loss of tocopherol for a similar level of oil degradation was higher as the degree of unsaturation decreased. Overall, the results showed that HOHPSO-, had a very high stability at frying temperatures and that mixtures of HOHPSO-, and HOHPSO-, would be an excellent alternative to fulfill the frying performance required by the processors and the vitamin,E content claimed by the consumers. [source] Involvement of astroglial ceramide in palmitic acid-induced Alzheimer-like changes in primary neuronsEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 8 2007Sachin Patil Abstract A high-fat diet has been shown to significantly increase the risk of the development of Alzheimer's disease (AD), a neurodegenerative disease histochemically characterized by the accumulation of amyloid beta (A,) protein in senile plaques and hyperphosphorylated tau in neurofibrillary tangles. Previously, we have shown that saturated free fatty acids (FFAs), palmitic and stearic acids, caused increased amyloidogenesis and tau hyperphosphorylaion in primary rat cortical neurons. These FFA-induced effects observed in neurons were found to be mediated by astroglial FFA metabolism. Therefore, in the present study we investigated the basic mechanism relating astroglial FFA metabolism and AD-like changes observed in neurons. We found that palmitic acid significantly increased de-novo synthesis of ceramide in astroglia, which in turn was involved in inducing both increased production of the A, protein and hyperphosphorylation of the tau protein. Increased amyloidogenesis and hyperphoshorylation of tau lead to formation of the two most important pathophysiological characteristics associated with AD, A, or senile plaques and neurofibrillary tangles, respectively. In addition to these pathophysiological changes, AD is also characterized by certain metabolic changes; abnormal cerebral glucose metabolism is one of the distinct characteristics of AD. In this context, we found that palmitic acid significantly decreased the levels of astroglial glucose transporter (GLUT1) and down-regulated glucose uptake and lactate release by astroglia. Our present data establish an underlying mechanism by which saturated fatty acids induce AD-associated pathophysiological as well as metabolic changes, placing ,astroglial fatty acid metabolism' at the center of the pathogenic cascade in AD. [source] Sterol-induced upregulation of phosphatidylcholine synthesis in cultured fibroblasts is affected by the double-bond position in the sterol tetracyclic ring structureFEBS JOURNAL, Issue 21 2000Petra Leppimäki We have examined how a specific enrichment of cultured fibroblasts with various sterols (cholesterol, lathosterol, 7-dehydrocholesterol, allocholesterol and dihydrocholesterol) regulate synthesis de novo of phosphatidylcholine, cholesterol and cholesteryl (or steryl) esters in human skin fibroblasts. When human skin fibroblasts were incubated for 1 h with 130 µm cholesterol/CyD complexes, the mass of cellular free cholesterol increased by 100 nmol·mg,1 protein (from 90 nmol·mg,1 to 190 nmol·mg,1 protein). A similar exposure of cells to different sterol/CyD complexes increased the cell sterol content between 38 and 181 nmol sterol per mg cell protein. In cholesterol-enriched cells, the rate of phosphatidylcholine synthesis was doubled compared to control cells, irrespective of the type of precursor used ([3H]choline, [3H]palmitic acid, or [14C]glycerol). Enrichment of fibroblasts with 7-dehydrocholesterol, allocholesterol, or dihydrocholesterol also upregulated phosphatidylcholine synthesis, whereas cells enriched with lathosterol failed to upregulate their phosphatidylcholine synthesis. The activity of membrane-bound CTP:phosphocholine cytidylyltransferase, the rate-limiting enzyme, was increased by 47 ± 4% in cholesterol-enriched cells whereas its activity was unchanged in lathosterol-enriched cells. Sterol enrichment with all tested sterols (including lathosterol) down-regulated acetate-incorporation into cholesterol, and upregulated sterol esterification in the sterol-enriched fibroblasts. Using 31P-NMR to measure the lamellar-to-hexagonal (L,,HII) phase transition in multilamellar lipid dispersions, lathosterol-containing membranes underwent their transition at significantly higher temperatures compared to membranes containing any of the other sterols. In a system with 1-palmitoyl-2-oleoyl- sn -glycero-3-phosphoethanolamine and either cholesterol or lathosterol (70 : 30 mol/mol), differential scanning calorimetry also revealed that the L,,HII -transition occurred at a higher temperature with lathosterol compared to either cholesterol, allocholesterol, or dihydrocholesterol. These findings together suggest that there may exist a correlation between the propensity of a sterol to stabilize the L,,HII -transition and its capacity to upregulate the activity of CTP:phosphocholine cytidylyltransferase in cells. [source] Identification of phosphatidylserine decarboxylases 1 and 2 from Pichia pastorisFEMS YEAST RESEARCH, Issue 6 2009Tamara Wriessnegger Abstract Genetic manipulation of lipid biosynthetic enzymes allows modification of cellular membranes. We made use of this strategy and constructed mutants in phospholipid metabolism of Pichia pastoris, which is widely used in biotechnology for expression of heterologous proteins. Here we describe identification of two P. pastoris phosphatidylserine decarboxylases (PSDs) encoded by genes homologous to PSD1 and PSD2 from Saccharomyces cerevisiae. Using P. pastoris psd1, and psd2, mutants we investigated the contribution of the respective gene products to phosphatidylethanolamine synthesis, membrane composition and cell growth. Deletion of PSD1 caused loss of PSD activity in mitochondria, a severe growth defect on minimal media and depletion of cellular and mitochondrial phosphatidylethanolamine levels. This defect could not be compensated by Psd2p, but by supplementation with ethanolamine, which is the substrate for the cytidine diphosphate (CDP),ethanolamine pathway, the third route of phosphatidylethanolamine synthesis in yeast. Fatty acid analysis showed selectivity of both Psd1p and Psd2p in vivo for the synthesis of unsaturated phosphatidylethanolamine species. Phosphatidylethanolamine species containing palmitic acid (16:0), however, were preferentially assembled into mitochondria. In summary, this study provides first insight into membrane manipulation of P. pastoris, which may serve as a useful method to modify cell biological properties of this microorganism for biotechnological purposes. [source] Volatile constituents of the essential oil of Nepeta ucrainica L. ssp. kopetdaghensis from IranFLAVOUR AND FRAGRANCE JOURNAL, Issue 2 2005K. Javidnia Abstract Composition of the volatile oil of Nepeta ucraininca L. ssp. kopetdaghensis (Lamiaceae) was investigated by GLC and GC-MS. Forty-one compounds representing 89.5% of the total oil were characterized. The main components of the oil were germacrene-D (39.7%), palmitic acid (10.8%), , -bourbonene (5.8%) and spathulenol (5.6%). Nepetalact-one isomers were not present in the essential oil of N. ucrainica L. ssp. kopetdaghensis. Copyright © 2004 John Wiley & Sons, Ltd. [source] Increased tumor necrosis factor ,,converting enzyme activity induces insulin resistance and hepatosteatosis in mice,HEPATOLOGY, Issue 1 2010Loredana Fiorentino Tumor necrosis factor ,,converting enzyme (TACE, also known as ADAM17) was recently involved in the pathogenesis of insulin resistance. We observed that TACE activity was significantly higher in livers of mice fed a high-fat diet (HFD) for 1 month, and this activity was increased in liver > white adipose tissue > muscle after 5 months compared with chow control. In mouse hepatocytes, C2C12 myocytes, and 3T3F442A adipocytes, TACE activity was triggered by palmitic acid, lipolysaccharide, high glucose, and high insulin. TACE overexpression significantly impaired insulin-dependent phosphorylation of AKT, GSK3, and FoxO1 in mouse hepatocytes. To test the role of TACE activation in vivo, we used tissue inhibitor of metalloproteinase 3 (Timp3) null mice, because Timp3 is the specific inhibitor of TACE and Timp3,/, mice have higher TACE activity compared with wild-type (WT) mice. Timp3,/, mice fed a HFD for 5 months are glucose-intolerant and insulin-resistant; they showed macrovesicular steatosis and ballooning degeneration compared with WT mice, which presented only microvesicular steatosis. Shotgun proteomics analysis revealed that Timp3,/, liver showed a significant differential expression of 38 proteins, including lower levels of adenosine kinase, methionine adenosysltransferase I/III, and glycine N -methyltransferase and higher levels of liver fatty acid-binding protein 1. These changes in protein levels were also observed in hepatocytes infected with adenovirus encoding TACE. All these proteins play a role in fatty acid uptake, triglyceride synthesis, and methionine metabolism, providing a molecular explanation for the increased hepatosteatosis observed in Timp3,/, compared with WT mice. Conclusion: We have identified novel mechanisms, governed by the TACE,Timp3 interaction, involved in the determination of insulin resistance and liver steatosis during overfeeding in mice. (HEPATOLOGY 2009.) [source] Effects of drying process on biochemical and microbiological quality of silverside (fish) Atherina lagunaeINTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 6 2010Salah Selmi Summary Changes in biochemical and microbiological quality of silverside during two drying process were investigated. Total fatty acid and amino acid contents in silverside were 5.42 and 10.9 g 100 g,1 fresh sample, respectively, in which, palmitic acid and glutamic acid were the most abundant. N-3 and n-6 polyunsaturated fatty acids (PUFA) levels were 0.59 and 0.62 g 100 g,1 fresh silverside respectively. After hot air and solar drying treatments, Peroxide value and thiobarbituric acid reactive substances of the total lipids increased significantly (P < 0.05) to reach 2.58 and 3.41 meq active O2 kg,1 oil and 0.87 and 1.27 mg MA kg,1 oil, respectively. Fatty acid profile of non-polar lipid remains unchanged during experimental drying process. However, PUFA (% of total fatty acids) of polar lipid decreased significantly (P < 0.05) during both drying process with lower levels in solar drying process. Drying conditions had a significant effect on the microbiological quality. Therefore, mesophiles and total coliforms increased within drying treatment with higher counts in solar drying process. [source] Effect of Planting Date on Seed Yield, Oil Content and Fatty Acid Composition of Safflower (Carthamus tinctorius) Cultivars Grown in the Mediterranean Region of TurkeyJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 5 2003B. Samanc Abstract A study was conducted to investigate the effect of different planting dates (25 April, 5 May and 15 May 1998 and 30 April, 15 May and 25 May 1999) on the seed yield, oil content and fatty acid composition of three safflower (Carthamus tinctorius) cultivars (Yenice 5-38, Dincer 5-118 and 5-154) grown in fields of the research facility of Akdeniz University in Antalya, Turkey. While seed yield, oil content, and palmitic acid, stearic acid, and oleic acid contents decreased, linoleic acid content increased from 50.86 to 55.72 % with delay in planting date. The effect of genotype on fatty acids was greater than that of environment. [source] The influence of dietary fish oil vs. sunflower oil on the fatty acid composition of plasma cholesteryl-esters in healthy, adult catsJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 11-12 2003E. A. Plantinga Summary The question addressed was whether the fatty acid composition of plasma cholesteryl esters (CEs) in cats reflects the intake of fatty acids. Diets containing either fish oil or sunflower oil were fed to six healthy, adult cats in a cross-over trial. The dry cat foods contained approximately 18.5% crude fat, of which two-third was in the form of the variable oil. Blood samples were collected at the end of each 4-week feeding period, and the fatty acid composition of plasma CEs and plasma concentrations of lipoproteins were determined. Consumption of the diet with fish oil was associated with significantly greater proportions of eicosapentaenoic acid, arachidonic acid, , -linolenic acid, oleic acid, palmitic acid and myristic acid in plasma CEs. The intake of fish oil instead of sunflower oil reduced the percentage of linoleic acid in CEs. The plasma concentrations of total cholesterol, high-density lipoprotein cholesterol, phospholipids and triglycerides were not affected by fish oil vs. sunflower oil feeding. [source] Effect of protein : energy ratio in milk replacers on growth performance of goat kidsJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 5-6 2002K-H. YEOM Two separate experiments were carried out to establish the effects of the protein : energy ratio in milk replacers on growth performance, plasma lipid concentrations and fatty acid composition in adipose tissue of male goat kids. In the first experiment there were 211 3-day- old goat kids and in the second experiment there were 121 kids aged 3,7 days. The animals were fed ad libitum for a period of 4 weeks on milk replacers containing either 11.5 or 9.5 g crude protein/MJ metabolizable energy. In essence, protein was exchanged with fat on a weight basis. Milk concentrations were increased from 160 to 190 g/l in experiment 1, from 150 to 180 g/l in experiment 2. There were significant increases in body weight and feed intake when the milk replacer with high protein : energy ratio was fed. Group mean average daily weight gain was 168 and 203 g for the groups with low and high dietary protein : energy ratio in experiment 1; for experiment 2 the values were 139 and 160 g. Average dry matter intake was 18 and 14% higher for the diet with high protein : energy ratio in experiments 1 and 2, respectively. There was no change in either feed conversion (feed : gain ratio) or energy conversion (weight gain : energy intake ratio). There were no consistent diet effects on plasma lipid concentrations. Dietary fatty acid composition was reflected by that of adipose tissue. The milk replacer with high protein : energy ratio produced a small increase in the contents of myristic and palmitic acid in adipose tissue. [source] Different apoptosis ratios and gene expressions in two human cell lines after sevoflurane anaesthesiaACTA ANAESTHESIOLOGICA SCANDINAVICA, Issue 9 2009S. KVOLIK Background: The aim of this study was to determine the effect of a single exposure of carcinoma cells (Caco-2 and HEp-2) to an anaesthetic gas mixture containing sevoflurane 3%, applied for a period of either 1 or 2 h, on the induction of apoptosis, propapototic gene expression and sphingomyelinase activity. Methods: Apoptosis was determined by flow cytometry. p53, caspase 3 and CYP2E1 gene expression was determined using reverse transcriptase polymerase chain reaction. Activities of acid (aSMase) and neutral sphingomyelinases (nSMase) were measured using methyl- 14C sphingomyeline, and for de novo ceramide and lipid synthesis [3H] palmitic acid was used. All results were compared with controls and analysed by Mann,Whitney and Kruskal,Wallis tests. Results: In the treated Caco-2 cells, the apoptotic ratio increased 24 h after anaesthesia (16.9%; P=0.04). The expression of both p53 and caspase-3 genes increased in Caco-2 and decreased in HEp-2 cells. The CYP2E1 gene expression was observed only in the Caco-2 cells. In control cells, the catalytic activity of aSMase was 2.3 times higher than that of nSMase activity. Decreased aSMase and nSMase activities were observed in Caco-2 cells 24 h after exposition. aSMase activity was halved (54.2%; P=0.06) in HEp-2 cells 24 h after anaesthesia. De novo ceramide synthesis correlated with SMase activity in Caco-2 cells. Conclusion: Sevoflurane anaesthesia induces late apoptosis in the colonic and laryngeal cancer cells investigated. Although the results obtained may indicate that an anaesthetic gas mixture containing sevoflurane induces p53-dependent apoptosis in the Caco-2 cells, the mechanism of apoptosis induction is unclear and remains to be elucidated. [source] Kinetics of esterification of palmitic acid with isopropanol using p -toluene sulfonic acid and zinc ethanoate supported over silica gel as catalystsJOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY, Issue 10 2004Roomana Aafaqi Abstract Kinetic data on the esterification of palmitic acid with isopropanol were obtained using homogeneous (para -toluene sulfonic acid, p -TSA) and heterogeneous (zinc ethanoate coated on silica gel, ZnA/SG) catalysts in a batch reactor. The ZnA/SG catalyst was prepared using a sol,gel technique. The esterification reaction was studied at different reaction temperatures (373,443 K), initial reactants molar ratio (1,5), catalyst loading (1,5 gcat dm,3) and water concentration in feed (0,15 vol%). A power law rate equation was used for homogeneous kinetics analysis. The Langmuir Hinshelwood Hougen Watson (LHHW) model was used for heterogeneous kinetics. The kinetic parameters of both models were obtained using Polymath software. The reaction parameters were used to obtain simulated values of conversion for both catalytic systems. The simulated values were compared with the experimental values and were in good agreement. Copyright © 2004 Society of Chemical Industry [source] LIPID, CHOLESTEROL AND FATTY ACID PROFILE OF SOME COMMERCIALLY IMPORTANT FISH SPECIES FROM SOUTH CASPIAN SEAJOURNAL OF FOOD BIOCHEMISTRY, Issue 4 2010S. PIRESTANI ABSTRACT The fatty acid, lipid, cholesterol and energy contents in five commercially important fish species from South Caspian Sea (common kilka, Caspian kutum, golden gray mullet, common carp and pike perch) were evaluated. The fatty acid compositions of these five fish species ranged from 28.99 to 41.05% saturated fatty acids, 40.99,56.25% monounsaturated fatty acids and 14.22,23.03% polyunsaturated fatty acids. Among these, those occurring in the highest proportions were palmitic acid (20.42,27.9%), palmitoleic acid (11.09,26.26%), oleic acid (16.1,36.94%), eicosapentaenoic acid (3.22,7.53%) and docosahexaenoic acid (3.86,11.36%). The lipid, cholesterol and energy contents ranged from 1.97% to 10.23%, 57,302 mg/100 g and 4365.4,5544.2 cal g/dm, respectively. The obtained Statistical results showed that in these fishes, many of the above mentioned indices had significant differences (P , 0.01) and the cluster analysis results of fatty acid compositions showed that common carp and pike perch had good similarity, followed by the Caspian kutum and golden gray mullet. However, common kilka did not show any similarity to others. PRACTICAL APPLICATIONS Fish consumption has been linked to health benefits such as reduced risk of coronary heart disease. This is largely attributed to the lipid, cholesterol, energy contents, fatty acid compositions and the polyunsaturated fatty acids present in fish oils. The ,-3 : ,-6, polyunsaturated fatty acid/saturated fatty acid and eicosapentaenoic acid + docosahexaenoic acid/C16 ratios are considered to be useful criteria for comparing relative nutritional and oxidation values of fish oils. [source] INHIBITION OF MICROORGANISMS IN SALAD DRESSING BY SUCROSE AND METHYLGLUCOSE FATTY ACID MONOESTERSJOURNAL OF FOOD PROCESSING AND PRESERVATION, Issue 4 2003CHING-MIAO YANG The antimicrobial activity of sucrose and methylglucose esters of medium to long chain fatty acids was studied with two microorganisms involved in the spoilage of salad dressings, Zygosaccharomyces bailii and Lactobacillus fructivorans. The microorganisms were inhibited to various degrees by 0.1, 0.5, and 1.0% synthesized sucrose or methylglucose monoesters using a modified broth dilution method. Sucrose monoesters were most inhibitory when the esterified fatty acid was myristic (C14) or palmitic acid (C16). Methylglucose monoesters with lauric (C12) or myristic acid (C14) exhibited greater inhibition than those with longer chain fatty acids. The least inhibition was generally observed with sucrose and methylglucose oleate (C18:1). Sucrose monoesters were usually more inhibitory than methylglucose monoesters of the same fatty acid, especially for palmitic and stearic (C18) acids. In salad dressing, 1% sucrose monoesters of lauric, myristic, or palmitic acid significantly (P < 0.05) inhibited the growth of Z. bailii and L. fructivorans, and were comparable with or more effective than 0.1% sodium benzoate. Z. bailii growth was nearly completely inhibited by sucrose laurate, myristate and palmitate by 9 days of salad dressing storage. Sucrose monoesters did not delay the lag phase of L. [source] Composition and Oxidative Stability of a Structured Lipid from Amaranth Oil in a Milk-Based Infant FormulaJOURNAL OF FOOD SCIENCE, Issue 2 2010Ashanty M. Pina-Rodriguez ABSTRACT:, Amaranth oil can be enzymatically modified to match breast milk fat analog requirements. We have developed a structured lipid (SL) from amaranth oil that, in combination with milk fat, delivers recommended amounts of docosahexaenoic acid (DHA) with palmitic acid specifically esterified at the,sn- 2 position of the triacylglycerol (TAG) backbone. The aim of this study was to study the final fatty acid (FA) contribution and oxidation stability of an infant formula prepared using the structured lipid DCAO (DHA-containing customized amaranth oil). DCAO was included as complementary fat in a "prototype" infant formula, and prepared in parallel with a "control" infant formula under the same processing conditions. The same ingredients but different complementary fat sources were used. A blend of the most commonly used vegetable oils (palm olein, soybean, coconut, and high-oleic sunflower oils) for infant formula was used instead of DCAO in the "control" formula. Additionally, "prototype" and "control" infant formulas were compared to a "commercial" product in terms of FA composition. The oxidative stability index (OSI) of the extracted fats from "prototype,""control," and "commercial" infant formulas were evaluated and compared to the OSI of the substrate fat replacers used. DCAO was the least stable compared to other fat analogs. The use of commercial antioxidants in DCAO containing products should prevent oxidation and therefore increase their stability. [source] Quality Attributes and Microbial Storage Stability of Caviar from Cultivated White Sturgeon (Acipenser transmontanus)JOURNAL OF FOOD SCIENCE, Issue 1 2010Joong-Han Shin ABSTRACT:, Caviar was prepared from white sturgeon (Acipenser transmontanus) roe by adjusting the water phase salt (WPS) to 4.0% to 6.3% by adding food grade NaCl. Fish were obtained from 2 different farms from the Inland Northwest (N,= 5). Salt was absorbed at a different rate and to a different extent by roe from different fish. The lipid content in the fish roe varied from 10.2% to 14.4% (w/w), with palmitic acid and oleic acid being the most abundant saturated and monounsaturated fatty acids present, respectively. The caviar contained high levels of polyunsaturated fatty acids (PUFA) (35% to 37%) with docosahexanoic acid being the most abundant ,-3 long chain fatty acid. There were no significant differences in microbial storage stability for caviars from different fish stored at 3 °C. However, for caviar stored at 7 °C, there was less growth of,Listeria monocytogenes,(using a cocktail of ATCC 19114, 7644, 19113 strains) in 2 samples (2B46 and 0F05) until day 20. In 2 other samples (453F and 2519), which had lower initial microbial loads, less overall microbial growth was observed, indicating that culture and harvest practices result in compositional differences between fish, which may impact both product composition and storage stability. [source] Effects of Bleaching on the Properties of Roasted Sesame OilJOURNAL OF FOOD SCIENCE, Issue 1 2005Inhwan Kim ABSTRACT: Improvement in quality of roasted sesame oil was studied. Roasted sesame oil was bleached at 70 °C, 85 °C, or 100 °C for 20 min with acid-activated clay at 0.5%, 1.0%, or 3.0% (w/w) and then centrifuging at 12096 × g at 4 °C for 10 min. The color of the roasted sesame oil became lighter and the viscosity of oil decreased by bleaching. Bleaching caused a significant increase in the smoke point of the oil, from 170 °C to a range of 183 °C to 191 °C. Bleaching increased palmitic acid and decreased linoleic acid contents of roasted sesame oil. Bleaching decreased free fatty acid (FFA) and conjugated dienoic acid (CDA) contents and carbonyl values (CV) of roasted sesame oil. The more the acid clay was used, the lower were the FFA and CDA contents and CV of the oil. Amount of acid clay in bleaching of roasted sesame oil had higher effects on the color, viscosity, smoke point, FFA and CDA contents, and CV of roasted sesame oil than the bleaching temperature. Bleaching did not show a significant effect on tocopherol contents of the sesame oil. Bleaching tended to decrease sesamolin contents and increase sesamol contents in the roasted sesame oil. As the amount of acid clay and the bleaching temperature increased, the contents of sesamin and sesamolin in the oil decreased while sesamol contents increased. [source] Molecular interactions of the neuronal GPI-anchored lipocalin LazarilloJOURNAL OF MOLECULAR RECOGNITION, Issue 5 2008Diego Sanchez Abstract Lazarillo, a glycoprotein involved in axon growth and guidance in the grasshopper embryo, is the only member of the lipocalin family that is attached to the cell surface by a GPI anchor. Recently, the study of Lazarillo homologous genes in Drosophila and mouse has revealed new functions in the regulation of lifespan, stress resistance and neurodegeneration. Here we report an analysis of biochemical properties of Lazarillo to gain insight into the molecular basis of its physiological function. Recombinant forms of the grasshopper protein were expressed in two different systems to test: (1) potential binding of several hydrophobic ligands; (2) protein,protein homophilic interactions; and (3) whether interaction with the function-blocking mAb 10E6 interferes with ligand binding. We tested 10 candidate ligands (retinoic acid, heme, bilirubin, biliverdin, ecdysterone, juvenile hormone, farnesol, arachidonic acid, linoleic acid and palmitic acid), and monitored binding using electrophoretic mobility shift, absorbance spectrum, and fluorimetry assays. Our work indicates binding to heme and retinoic acid, resulting in increased electrophoretic mobility, as well as to fatty acids, resulting in multimerization. Retinoic acid and fatty acids binding were confirmed by fluorescence titration, and heme binding was confirmed with absorbance spectrum assays. We demonstrate that Lazarillo oligomerizes in solution and can form clusters in the plasma membrane when expressed and GPI-anchored to the cell surface, however it is unable to mediate cell,cell adhesion. Finally, by ligand-mAb competition experiments we show that ligand-binding alone cannot be the key factor for Lazarillo to perform its function during axonal growth in the grasshopper embryo. Copyright © 2008 John Wiley & Sons, Ltd. [source] Phase equilibrium in supercritical CO2 mixtures using a modified Kwak-Mansoori mixing ruleAICHE JOURNAL, Issue 2 2004José O. Valderrama Abstract The mixing rules proposed by Kwak and Mansoori for the Peng-Robinson equation of state have been modified to describe vapor-liquid equilibrium in mixtures that contain supercritical CO2. Data in the literature for nine binary liquid,vapor systems containing supercritical CO2 are used for testing the modified models. The systems studied were binary mixtures containing carbon dioxide with lauric acid, palmitic acid, oleic acid, linoleic acid, 1-octanol, 1-decanol, 2-methyl-1-pentanol, ,-pinene, and limonene. The modifications studied included the introduction of a nonquadratic mixing rule proposed by one of the authors and a different interaction parameter for the volume constant of the equation of state. Compared to results in the literature, the Kwak-Mansoori combining rules and the proposed empirical modifications give lower deviations in correlating the solute concentration in the vapor phase, the most important variable for the design of supercritical extraction processes. Contrary to arguments found in the literature, it is also demonstrated that a severe test for an equation of state and its mixing rules is the correlation of the solute concentration in vapor,liquid mixtures. © 2004 American Institute of Chemical Engineers AIChE J, 50: 480,488, 2004 [source] A laser imaging and neutron reflection investigation into the monolayer behaviour of fatty acids used for taste masking microspheresJOURNAL OF PHARMACEUTICAL SCIENCES, Issue 5 2008Sheng Qi Abstract Fatty acid microspheres have been used for taste masking purposes whereby the drug is preferentially released in the lower gastrointestinal tract, although the mechanisms involved are poorly understood. In this study, we use a combination of surface pressure measurements, Brewster angle microscopy (BAM) and neutron reflectivity measurements to study the phase miscibility and escaping tendency from mixed stearic and palmitic acid films with a view to relating this to drug dissolution behaviour. It was noted that mixed systems showed considerably greater film interaction and instability than those composed of the pure lipid, especially in alkaline media. BAM studies were able to identify a range of phase separated structures for both the pure and mixed systems. Neutron reflectivity studies indicated a marked selective dissolution of palmitic acid into the subphase as a function of time and allowed quantification of the rate of dissolution of this species. It is concluded that the fatty acids are interacting within the monolayer and in addition the palmitic acid is escaping the mixed monolayers and dissolving into the alkali subphase. These findings have strong relevance for understanding the mechanism of drug release from the associated microspheres. © 2007 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 97: 1864,1877, 2008 [source] Apoptosis inducing activity of viscin, a lipophilic extract from Viscum album L.JOURNAL OF PHARMACY AND PHARMACOLOGY: AN INTERNATI ONAL JOURNAL OF PHARMACEUTICAL SCIENCE, Issue 1 2005K. Urech Detection of antiproliferative activity and bioactivity-guided fractionation of viscin, a lipophilic extract from Viscum album L., led to the isolation of betulinic acid, oleanolic acid and ursolic acid as active components. Viscin, betulinic acid, oleanolic acid and ursolic acid inhibited growth and induced apoptotic cell death in Molt4, K562 and U937 leukaemia cells. The growth inhibitory effect of viscin was more pronounced in Molt4 and U937 cells (IC50 (concentration that inhibited cell proliferation by 50%): 118 ± 24 and 138 ± 24 ,g mL,1) than in K562 cells (IC50: 252 ± 37 ,g mL,1). Oleanolic acid was the least effective in all cell lines (7.5,45.5% inhibition at 10 ,g mL,1) and ursolic acid the most active in Molt4 and U937 cells (81.8 and 97.8% inhibition, respectively, at 5 ,g mL,1). A dose-dependent loss of membrane phospholipid asymmetry associated with apoptosis was induced in all cell lines as shown in flow cytometry by the externalization of phosphatidylserine and morphological changes in cell size and granularity. There were differences in individual cell lines' response towards the apoptosis-inducing effect of viscin, betulinic acid, oleanolic acid and ursolic acid. The triterpenoids ,-amyrin, ,-amyrinacetate, lupeol, lupeolacetate, ,-sitosterol and stigmasterol, and the fatty acids oleic acid, linoleic acid, palmitic acid and stearic acid were also present in the lipophilic extract. [source] | |||||||||||||||||||||||||||||||