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Terms modified by Parrots Selected AbstractsTWO NEW PARROTS (PSITTACIFORMES) FROM THE LOWER EOCENE FUR FORMATION OF DENMARKPALAEONTOLOGY, Issue 3 2008DAVID M. WATERHOUSE Abstract:, Two new fossil psittaciform birds from the Lower Eocene ,Mo Clay' (Fur Formation) of Denmark (c. 54 Ma) are described. An unnamed specimen is assigned to the extinct avian family of stem-group parrots, Pseudasturidae (genus and species incertae sedis), while a second (Mopsitta tanta gen. et sp. nov.) is the largest fossil parrot yet known. Both specimens are the first fossil records of these birds from Denmark. Although the phylogenetic position of Mopsitta is unclear (it is classified as family incertae sedis), this form is phylogenetically closer to Recent Pstittacidae than to other known Palaeogene psittaciforms and may, therefore, represent the oldest known crown-group parrot. [source] Phylogeny and biogeography of Yellow-headed and Blue-fronted Parrots (Amazona ochrocephala and Amazona aestiva) with special reference to the South American taxaIBIS, Issue 3 2007CAMILA C. RIBAS The Yellow-headed Parrot (Amazona ochrocephala) has a broad Neotropical distribution, ranging from Mexico to the Amazon Basin, and a history of complex taxonomy and controversial species limits. Recent molecular analyses have started to clarify the taxonomic arrangement of the complex, but have not included a representative geographical sampling from South America. These studies have shown that the Yellow-headed complex can be divided into three main lineages, and seems to be paraphyletic, due to the inclusion of the Blue-fronted Parrot (Amazona aestiva) that occurs in central South America. Here we present a phylogenetic analysis based on mitochondrial DNA sequences of 45 representatives of the Yellow-headed complex from South and Central America, plus 13 Blue-fronted individuals from different localities in South America. Our analyses recover the three primary lineages found previously in the Yellow-headed complex, show that there is genetic structure in the South American lineage, which can be divided into two well-supported, closely related clades, and demonstrate that Blue-fronted samples are distributed in both clades. Differentiation of South American Blue-fronted and Yellow-headed Parrot populations does not correspond to the plumage differences used to distinguish the Blue-fronted Parrot from the Yellow-headed Parrot, nor to plumage differences used to distinguish among South American Yellow-headed subspecies. This suggests that traditional taxonomy based on plumage characters needs revision, and that this may be an interesting example of ongoing divergence-with-gene-flow related to the forest/open area ecotone in southern Amazonia. [source] Phlebotomus (Adlerius) halepensis vector competence for Leishmania major and Le. tropicaMEDICAL AND VETERINARY ENTOMOLOGY, Issue 3 2003J. Sádlová Abstract., In Eurasia, phlebotomine sandflies of the subgenus Adlerius (Diptera: Psychodidae) comprise about 20 known species. Some are suspected vectors of visceral leishmaniasis (VL) and at least one species has been implicated as a vector of cutaneous leishmaniasis (CL). We tested Phlebotomus (Adlerius) halepensis Theodor (Jordan strain) for CL vector competence, compared with three standard vectors: Phlebotomus (Phlebotomus) duboscqi N-L. from Senegal, Phlebotomus (Paraphlebotomus) sergenti Parrot from Turkey and the Neotropical Lutzomyia longipalpis (L. & N) (Jacobina strain). Sandfly females were membrane-fed on amastigote suspensions of Leishmania major Y. & S. and Le. tropica (Wright) (Kinetoplastida: Trypanosomatidae) and examined for parasite development 3, 6 and 10 days post-infection. Phlebotomus halepensis showed high susceptibility to both leishmanias, supporting typical suprapylarian parasite development similar to the other vectors. Phlebotomus halepensis infection rates were ,90% for Le. major and ,80% for Le. tropica, with high parasite densities. Development of infections was relatively fast, colonizing the thoracic midgut by 6 days post-bloodmeal in every case and reaching the stomodeal valve in >80% of flies. In late-stage infections, 10 days post-bloodmeal, nearly all P. halepensis females had cardia and stomodeal valve filled with very high numbers of parasites and some Le. tropica -infected females had promastigotes in the pharynx and proboscis. Host choice experiments in the laboratory showed that P. halepensis females fed readily on rat or rabbit and preferred the human forearm. In view of its vector competence and partial anthropophily, we infer that P. halepensis is a potential vector of cutaneous as well as visceral leishmaniases. [source] Nesting Habitat of the Lilac-crowned Parrot in a Modified Landscape in MexicoBIOTROPICA, Issue 3 2009Tiberio C. Monterrubio-Rico ABSTRACT Parrot populations are being increasingly pressured to occupy modified or fragmented landscapes, yet little is known of the habitat requirements of most species, particularly with regard to the effects on breeding habitat. We evaluated nesting habitat of the lilac-crowned parrot Amazona finschi in the modified landscape of coastal Michoacan in Mexico. We located 90 parrot nests in 12 tree species in Michoacan, with lilac-crowned parrots presenting a narrow niche-breadth of tree species used for nesting. Considering an additional 82 nest trees recorded for lilac-crowned parrots in Jalisco, we determined a 51 percent similarity in cavity resource use by parrots in the two dry forest regions. Overall, the predominant nest tree species with 76 percent of nests were Astronium graveolens, Piranhea mexicana, Brosimum alicastrum, and Tabebuia spp., all characteristic of semi-deciduous forest. Only 8 percent of nests occurred in trees characteristic of deciduous forest. Parrots utilized large trees with canopy level cavities as nest sites, and preferred conserved semi-deciduous forest for nesting, with fewer nests than expected in deciduous forest and transformed agricultural land. Nest areas in semi-deciduous forest occurred on significantly steeper terrain, as remnant semi-deciduous forest is restricted to steep ridges and canyons. Those parrot nests in modified habitats and forest patches were located near to continuous forest, with nest trees in open agricultural land being significantly closer to continuous forest than nests in disturbed forest patches. These results demonstrate the importance of conserved semi-deciduous forest as breeding habitat for the threatened, endemic lilac-crowned parrot, making wild populations of the species vulnerable to the high rate of transformation and fragmentation of tropical dry forest. RESUMEN Las poblaciones de Psitácidos están siendo presionadas a ocupar paisajes modificados, sin embargo poco se conoce sobre los requerimientos de hábitat para la mayoría de las especies, particularmente con respecto a los efectos sobre sus hábitats de anidación. Evaluamos el hábitat de anidación del loro corona lila (Amazona finschi) en el paisaje modificado de la costa de Michoacán en México. Localizamos 90 sitios de anidación en 12 especies de árboles en Michoacan, encontrando que el loro corona lila presentó una estrecha amplitud de nicho en el uso de especies de árboles para anidar. Considerando un adicional 82 árboles-nido del loro corona lila en Jalisco, determinamos un 51% de similitud en utilización del recurso de cavidades por los loros en los dos regiones de bosque seco. Los árboles claves, con 76% de los nidos, fueron Astronium graveolens, Piranhea mexicana, Brosimum alicastrum, y Tabebuia spp., todos característicos del bosque subcaducifolio. Solo 8% de los nidos se encontraron en arboles características del bosque caducifolio. Los loros utilizaron árboles grandes con cavidades en el dosel como sitios de anidación, y prefirieron el bosque subcaducifolio conservado para anidar, con un menor número de nidos que lo esperado en el bosque caducifolio y tierras agropecuarias. Las áreas de anidación en bosque subcaducifolio ocurrieron sobre pendientes significativamente más inclinadas, ya que los remanentes de este tipo de vegetación están actualmente restringidos a pendientes inclinadas y cañadas. Aquellos nidos en hábitats modificados y parches pequeños de vegetación se localizaron cerca de áreas de bosque continuo, con los árboles-nido que ocurrieron en terrenos agropecuarios significativamente más cerca de los bosques continuos, que los que ocurrieron en fragmentos de bosques perturbados. Estos resultados destacan la importancia del bosque subcaducifolio conservado como hábitat de anidación para esta especie de loro endémico y amenazado, haciendo a sus poblaciones silvestres vulnerables al alto nivel de transformación y fragmentación del bosque tropical seco. [source] Effects of Male Vocal Learning on Female Behavior in the Budgerigar, Melopsittacus undulatusETHOLOGY, Issue 10 2005Arla G. Hile Parrots are unusual among birds and animals in general in the extent of their ability to learn new vocalizations throughout life and irrespective of season. The budgerigar (Melopsittacus undulatus), a small parrot that is well suited for laboratory studies, has been the subject of numerous studies investigating the neurobiology of vocal learning. To date, few studies have focused on the function of vocal imitation by parrots. Previous work from our research group has shown that vocal imitation in budgerigars is sex-biased, as males paired with females learn vocalizations from their new mates, but not vice versa. This bias led us to hypothesize that vocal learning has a reproductive function. To test this hypothesis, we conducted two experiments. In the first experiment, we tutored males so that they could produce a call similar to one shared by a group of experimental females. The experimental females were then presented with one of the tutored males and another, equally unfamiliar, male that had not been tutored. We found that the females spent a greater proportion of time in proximity of, and made more affiliative displays toward, the tutored males. In the second experiment, seven males received small bilateral brain lesions that disrupt vocal learning. These males and an equal number of control males were then released into an aviary containing females and reproductive resources. We found that lesioned and control males were equally successful in obtaining social mates, but females mated to lesioned males were more likely to engage in extra-pair activities. These experiments indicate that a male's ability to imitate a female's call can influence the sexual behavior of the female even though lack of imitation ability does not appear to influence social pairing. We hypothesize that mate choice in budgerigars has multiple stages. Upon meeting a strange male, a female quickly assesses its ability for social acquisition of calls by the presence or absence of a call type similar to its own in its repertoire. As courtship proceeds into pair formation, the female assesses the ability of male to learn more directly by the extent of the male's perfection of imitation. [source] Nest-site fidelity and cavity reoccupation by Blue-fronted Parrots Amazona aestiva in the dry Chaco of ArgentinaIBIS, Issue 1 2009IGOR BERKUNSKY The frequency of cavity reoccupation in secondary cavity nesters depends on several factors including quality of cavities, degree of nest-site fidelity, competition with other cavity nesters and availability of new cavities. Blue-fronted Parrots Amazona aestiva are secondary cavity nesters that live in subtropical forests and savannas of central South America. We examined the characteristics of the trees and cavities used by this species in a protected area of the dry Chaco of Argentina and estimated nest-site fidelity and cavity reoccupation. We also assessed whether the probability of cavity reoccupation was associated with cavity characteristics and nesting success during the previous year. Nest-site fidelity of banded females was 68% and cavity reoccupation by banded and unbanded individuals 62%. Probability of reoccupation was associated with wall thickness and depth of the cavity, and was lower if the nest failed the previous year than if it was successful. The high rate of cavity reoccupation in Blue-fronted Parrots is largely attributable to strong nest-site fidelity and may reflect preferences for cavities whose characteristics are associated with higher nesting success. [source] Phylogeny and biogeography of Yellow-headed and Blue-fronted Parrots (Amazona ochrocephala and Amazona aestiva) with special reference to the South American taxaIBIS, Issue 3 2007CAMILA C. RIBAS The Yellow-headed Parrot (Amazona ochrocephala) has a broad Neotropical distribution, ranging from Mexico to the Amazon Basin, and a history of complex taxonomy and controversial species limits. Recent molecular analyses have started to clarify the taxonomic arrangement of the complex, but have not included a representative geographical sampling from South America. These studies have shown that the Yellow-headed complex can be divided into three main lineages, and seems to be paraphyletic, due to the inclusion of the Blue-fronted Parrot (Amazona aestiva) that occurs in central South America. Here we present a phylogenetic analysis based on mitochondrial DNA sequences of 45 representatives of the Yellow-headed complex from South and Central America, plus 13 Blue-fronted individuals from different localities in South America. Our analyses recover the three primary lineages found previously in the Yellow-headed complex, show that there is genetic structure in the South American lineage, which can be divided into two well-supported, closely related clades, and demonstrate that Blue-fronted samples are distributed in both clades. Differentiation of South American Blue-fronted and Yellow-headed Parrot populations does not correspond to the plumage differences used to distinguish the Blue-fronted Parrot from the Yellow-headed Parrot, nor to plumage differences used to distinguish among South American Yellow-headed subspecies. This suggests that traditional taxonomy based on plumage characters needs revision, and that this may be an interesting example of ongoing divergence-with-gene-flow related to the forest/open area ecotone in southern Amazonia. [source] Do Meyer's Parrots Poicephalus meyeri benefit pollination and seed dispersal of plants in the Okavango Delta, Botswana?AFRICAN JOURNAL OF ECOLOGY, Issue 3 2010Rutledge S. Boyes Abstract Recent studies of new world parrots repeatedly document, with few exceptions, that parrots are wasteful and destructive predispersal seed predators that are unlikely to contribute towards pollination and seed dispersal. Few detailed studies, however, have assessed the contribution of African parrots to forest ecology by quantifying the potential net benefit of seed and flower predation by parrots for most tree species in their diet. Due to the incidence of pollen on the heads of Meyer's Parrots when feeding on Leguminosae flowers and the dispersal of viable seeds to the ground during seed predation, we compared destruction rates, when feeding on pods, fruits and flowers, with dispersal rates of viable seeds to the ground and frequency of head contact with reproductive apparatus to estimate net benefit from Meyer's Parrot feeding activity. Meyer's Parrots were not implicated in endo- or epizoochory, but they dropped uneaten fruit pulp and seeds to the ground during feeding bouts, thus providing ripe, undamaged seeds to secondary seed dispersers. This link with forest recruitment was weak, as all tree species utilized by Meyer's Parrots either had more significant primary dispersal agents or were primarily wind-dispersed. In most cases, the negative effect of seed predation outweighed any positive effects in terms of dispersal, whereby almost three times more seeds were consumed or destroyed than were dispersed to the ground. Significantly, only Sclerocarya birrea caffra recorded marginal net dispersal benefit from utilization by Meyer's Parrots. Due to low relative resource abundance and high destruction rate, feeding activity on Berchemia discolor may be significant enough to influence its spatial distribution and abundance. Utilization of flowers of Kigelia africana and Adansonia digitata by parrots likely had a significant negative impact on pollination. Feeding on Acacia nigrescens flowers, however, was potentially advantageous to their pollination. We conclude that Poicephalus parrots are net consumers of ripe, undamaged seeds and flowers, thus having an overall negative impact on forest recruitment in subtropical Africa. Résumé Les études récentes des perroquets du nouveau monde disent toujours, à quelques exceptions près, que les perroquets sont des prédateurs qui gaspillent et détruisent les semences avant leur dispersion et qu'ils sont donc peu susceptibles de contribuer à la fertilisation et à la dispersion des graines. Cependant, peu d'études détaillées ont évalué la contribution des perroquets africains à l'écologie des forêts en quantifiant le bénéfice net potentiel de la consommation des graines et des fleurs pour la plupart des espèces d'arbres qui entrent dans leur régime alimentaire. Sachant que la tête des perroquets de Meyer se charge de pollen lorsqu'ils se nourrissent de fleurs de légumineuses et qu'ils dispersent des semences viables sur le sol lorsqu'ils en consomment, nous comparons le taux de destruction, lorsqu'ils mangent des gousses, des fruits et des fleurs, avec le taux de dispersion de semences viables sur le sol et la fréquence des contacts de leur tête avec l'appareil reproducteur des plantes, pour estimer le bénéfice net de l'alimentation du perroquet de Meyer. Ces perroquets n'étaient pas impliqués dans l'endo- ou l'épizoochorie, mais lorsqu'ils mangeaient, ils laissaient tomber de la pulpe et des graines de fruits non consommées, fournissant ainsi des semences intactes à des disperseurs secondaires. Ce rapport avec le recrutement des forêts était faible dans la mesure où toutes les semences des espèces d'arbres fréquentées par les perroquets de Meyer soit avaient des agents de dispersion primaires plus importants, soit étaient principalement dispersées par le vent. Dans la plupart des cas, l'effet négatif de la consommation des semences dépassait tout effet positif en termes de dispersion, et presque trois fois plus de graines étaient mangées ou détruites que dispersées sur le sol. Il est intéressant de noter que seul Sclerocarya birrea caffra présentait un bénéfice net de dispersion marginal dûà la fréquentation des perroquets de Meyer. En raison de la faible abondance relative de ressources et du taux de destruction élevé, la consommation de Berchemia discolor pourrait être assez significative pour influencer sa distribution spatiale et son abondance. La consommation des fleurs de Kigelia africana et d'Adansonia digitata par les perroquets avait probablement un impact négatif sur leur pollinisation. Mais la consommation des fleurs d'Acacia nigrescensétait peut-être avantageuse pour la pollinisation. Nous concluons que les perroquets Poicephalus sont des consommateurs nets de graines et de fleurs mûres et intactes, et qu'ils ont donc un impact global négatif sur le recrutement des forêts en Afrique subtropicale. [source] Parrots Take it with a Grain of Salt: Available Sodium Content May Drive Collpa (Clay Lick) Selection in Southeastern PeruBIOTROPICA, Issue 3 2009Luke L. Powell ABSTRACT Soils from 18 parrot collpas (,clay licks') in southeastern Peru averaged four times more available sodium than uneaten control soils. Collpa soils contained marginally more clay than control sites and clay content was uncorrelated with available sodium content. Parrots may select and ingest soils based on available sodium content. RESUMEN Suelos de 18 colpas de loros del sureste del Perú promediaron cuatro veces más sodio disponible que suelos control no consumidos. Suelos de colpas fueron un poco más arcillosos que los suelos control; además el contenido de arcilla no presentó correlación con el contenido de sodio disponible. Concluimos que la selección y consumo de suelos por loros se basa en el contenido de sodio disponible. [source] Nesting Habitat of the Lilac-crowned Parrot in a Modified Landscape in MexicoBIOTROPICA, Issue 3 2009Tiberio C. Monterrubio-Rico ABSTRACT Parrot populations are being increasingly pressured to occupy modified or fragmented landscapes, yet little is known of the habitat requirements of most species, particularly with regard to the effects on breeding habitat. We evaluated nesting habitat of the lilac-crowned parrot Amazona finschi in the modified landscape of coastal Michoacan in Mexico. We located 90 parrot nests in 12 tree species in Michoacan, with lilac-crowned parrots presenting a narrow niche-breadth of tree species used for nesting. Considering an additional 82 nest trees recorded for lilac-crowned parrots in Jalisco, we determined a 51 percent similarity in cavity resource use by parrots in the two dry forest regions. Overall, the predominant nest tree species with 76 percent of nests were Astronium graveolens, Piranhea mexicana, Brosimum alicastrum, and Tabebuia spp., all characteristic of semi-deciduous forest. Only 8 percent of nests occurred in trees characteristic of deciduous forest. Parrots utilized large trees with canopy level cavities as nest sites, and preferred conserved semi-deciduous forest for nesting, with fewer nests than expected in deciduous forest and transformed agricultural land. Nest areas in semi-deciduous forest occurred on significantly steeper terrain, as remnant semi-deciduous forest is restricted to steep ridges and canyons. Those parrot nests in modified habitats and forest patches were located near to continuous forest, with nest trees in open agricultural land being significantly closer to continuous forest than nests in disturbed forest patches. These results demonstrate the importance of conserved semi-deciduous forest as breeding habitat for the threatened, endemic lilac-crowned parrot, making wild populations of the species vulnerable to the high rate of transformation and fragmentation of tropical dry forest. RESUMEN Las poblaciones de Psitácidos están siendo presionadas a ocupar paisajes modificados, sin embargo poco se conoce sobre los requerimientos de hábitat para la mayoría de las especies, particularmente con respecto a los efectos sobre sus hábitats de anidación. Evaluamos el hábitat de anidación del loro corona lila (Amazona finschi) en el paisaje modificado de la costa de Michoacán en México. Localizamos 90 sitios de anidación en 12 especies de árboles en Michoacan, encontrando que el loro corona lila presentó una estrecha amplitud de nicho en el uso de especies de árboles para anidar. Considerando un adicional 82 árboles-nido del loro corona lila en Jalisco, determinamos un 51% de similitud en utilización del recurso de cavidades por los loros en los dos regiones de bosque seco. Los árboles claves, con 76% de los nidos, fueron Astronium graveolens, Piranhea mexicana, Brosimum alicastrum, y Tabebuia spp., todos característicos del bosque subcaducifolio. Solo 8% de los nidos se encontraron en arboles características del bosque caducifolio. Los loros utilizaron árboles grandes con cavidades en el dosel como sitios de anidación, y prefirieron el bosque subcaducifolio conservado para anidar, con un menor número de nidos que lo esperado en el bosque caducifolio y tierras agropecuarias. Las áreas de anidación en bosque subcaducifolio ocurrieron sobre pendientes significativamente más inclinadas, ya que los remanentes de este tipo de vegetación están actualmente restringidos a pendientes inclinadas y cañadas. Aquellos nidos en hábitats modificados y parches pequeños de vegetación se localizaron cerca de áreas de bosque continuo, con los árboles-nido que ocurrieron en terrenos agropecuarios significativamente más cerca de los bosques continuos, que los que ocurrieron en fragmentos de bosques perturbados. Estos resultados destacan la importancia del bosque subcaducifolio conservado como hábitat de anidación para esta especie de loro endémico y amenazado, haciendo a sus poblaciones silvestres vulnerables al alto nivel de transformación y fragmentación del bosque tropical seco. [source] Effects of Male Vocal Learning on Female Behavior in the Budgerigar, Melopsittacus undulatusETHOLOGY, Issue 10 2005Arla G. Hile Parrots are unusual among birds and animals in general in the extent of their ability to learn new vocalizations throughout life and irrespective of season. The budgerigar (Melopsittacus undulatus), a small parrot that is well suited for laboratory studies, has been the subject of numerous studies investigating the neurobiology of vocal learning. To date, few studies have focused on the function of vocal imitation by parrots. Previous work from our research group has shown that vocal imitation in budgerigars is sex-biased, as males paired with females learn vocalizations from their new mates, but not vice versa. This bias led us to hypothesize that vocal learning has a reproductive function. To test this hypothesis, we conducted two experiments. In the first experiment, we tutored males so that they could produce a call similar to one shared by a group of experimental females. The experimental females were then presented with one of the tutored males and another, equally unfamiliar, male that had not been tutored. We found that the females spent a greater proportion of time in proximity of, and made more affiliative displays toward, the tutored males. In the second experiment, seven males received small bilateral brain lesions that disrupt vocal learning. These males and an equal number of control males were then released into an aviary containing females and reproductive resources. We found that lesioned and control males were equally successful in obtaining social mates, but females mated to lesioned males were more likely to engage in extra-pair activities. These experiments indicate that a male's ability to imitate a female's call can influence the sexual behavior of the female even though lack of imitation ability does not appear to influence social pairing. We hypothesize that mate choice in budgerigars has multiple stages. Upon meeting a strange male, a female quickly assesses its ability for social acquisition of calls by the presence or absence of a call type similar to its own in its repertoire. As courtship proceeds into pair formation, the female assesses the ability of male to learn more directly by the extent of the male's perfection of imitation. [source] The clergyman's wife and the parrotEVOLUTIONARY ANTHROPOLOGY, Issue 1 2006Kenneth Weiss First page of article [source] Reactions of nomadic and resident parrot speciesINTERNATIONAL ZOO YEARBOOK, Issue 1 2000C. METTKE-HOFMANN The exploratory behaviour of six species of lorikeet (Loriini) and four species of broad-tailed parrot (Platycercini), which differ in their migratory behaviour, was tested to investigate whether residents and nomads show different reactions to environmental enrichment. For each species, seven pairs of birds were tested in a familiar aviary. The test was performed on 2 days, separated by a resting period of 2 days. Three unknown objects were brought into the aviary for 24 hours on day 1 and 6 hours on day 2. The results showed that more resident birds contacted the objects than nomadic birds and that residents showed shorter latencies until first contact than nomads. No differences between groups occurred in the duration of exploration but there was a positive correlation between duration of exploration and the tendency of the species to exhibit plucking behaviour. The results suggest that object presentation is a useful tool in supporting activity in resident as well as nomadic Psittacidae. [source] Phylogenetic analysis by 16S rDNA gene sequence comparison of avian taxa of Bisgaard and characterization and description of two new taxa of PasteurellaceaeJOURNAL OF APPLIED MICROBIOLOGY, Issue 2 2003H. Christensen Abstract Aims: Characterization and classification of members of Pasteurellaceae isolated from birds by extended phenotypic characterization and 16S rDNA gene sequence comparison. Methods and Results: A total of 95 avian isolates were subjected to extended phenotypic characterization. Thirteen bacterial strains selected from main phenotypic clusters and isolated from parrot, parakeet, budgerigar, partridge, pheasant, chicken, duck, hawk and gull were subsequently characterized by 16S rDNA gene sequencing. Eight of the sequenced strains were classified with six taxa of Bisgaard of which two (34 and 40) have not been published before, and the properties of four others (14, 22, 26 and 32) changed upon the characterization of these new isolates. Of the remaining strains, one was identified as a phenotypic variant in maltose and dextrin of Pasteurella gallinarum another as a trehalose positive variant of taxon 3 of Bisgaard. The remaining three strains sequenced were not closely related to existing taxa of Pasteurellaceae. However, they were found to belong to the Avian cluster with 92,97% 16S rDNA gene sequence similarity. Conclusion: The study allowed the classification of bacteria isolated from birds by the integrated use of extended phenotypic characterization and 16S rDNA gene sequence analysis. Only the application of 16S rDNA gene sequencing allows a correct identification of variant strains. Significance and Impact of the Study: The description of new taxa within the bacterial family Pasteurellaceae will subsequently allow additional isolates of these taxa to be identified and improve the diagnosis and epidemiological understanding of bacteria causing disease in birds. [source] Eucalyptus pollen grain emptying by two Australian nectarivorous psittacinesJOURNAL OF AVIAN BIOLOGY, Issue 3 2001B. D. Gartrell The relative importance of pollen as a source of protein to vertebrates is controversial. In nectarivorous psittacine birds, field studies support its importance, but an experimental study in a nectarivorous parrot showed that less than 7% of pollen grains were emptied. We investigated pollen grain emptying by two nectarivorous Australian parrots, the Swift Parrot Lathamus discolor and the Musk Lorikeet Glossopsitta concinna. We used a controlled experiment, and examined pollen located at different levels through the alimentary tract of wild L. discolor. There was significant emptying of pollen grains (x=45.4%±1.91 s.e.) by all birds in the experimental trials. There was also a progressive increase in the percentage of pollen grains emptied at different sites along the alimentary tract in wild birds (crop x=24.2%±4.44 s.e., proventriculus x=34.0%±7.29 s.e., duodenum x=54.3%±5.42 s.e. and distal intestine x=64.2%±4.68 s.e.). The percentage of pollen grains emptied by captive L. discolor in the experimental trial (x=44.1%±2.77 s.e.) was not significantly different from that found in wild L. discolor (x=40.3%±4.25 s.e.). Both species of nectarivorous parrot were able to rapidly ingest large quantities of Eucalyptus pollen and appeared to empty the pollen grains efficiently. Eucalyptus pollen appears to be an important source of protein for these birds. [source] TWO NEW PARROTS (PSITTACIFORMES) FROM THE LOWER EOCENE FUR FORMATION OF DENMARKPALAEONTOLOGY, Issue 3 2008DAVID M. WATERHOUSE Abstract:, Two new fossil psittaciform birds from the Lower Eocene ,Mo Clay' (Fur Formation) of Denmark (c. 54 Ma) are described. An unnamed specimen is assigned to the extinct avian family of stem-group parrots, Pseudasturidae (genus and species incertae sedis), while a second (Mopsitta tanta gen. et sp. nov.) is the largest fossil parrot yet known. Both specimens are the first fossil records of these birds from Denmark. Although the phylogenetic position of Mopsitta is unclear (it is classified as family incertae sedis), this form is phylogenetically closer to Recent Pstittacidae than to other known Palaeogene psittaciforms and may, therefore, represent the oldest known crown-group parrot. [source] Darstellung der Nerven am Flügel von Psittaciden (Amazone, Graupapagei) mit digitaler Phototechnik ,ANATOMIA, HISTOLOGIA, EMBRYOLOGIA, Issue 4 2000G. Hummel Representation of the nerves of the wings in the blue-fronted Amazon and the African grey parrot using a digital photographic technique In four blue-fronted Amazon and five African grey parrots, the nerves innervating the muscles of the wings were carefully dissected and described. The preparation steps were recorded with a digital camera. This technique has now advanced so far that the image quality is almost the same as that of a normal camera. The pictures are stored in a computer. They can be enhanced and published via the Internet. Zusammenfassung Bei 4 Blaustirnamazonen und 5 Graupapageien werden die Nerven, welche die Flügelmuskulatur versorgen, sorgfältig präpariert und beschrieben. Die einzelnen Präparationsschritte werden mit einer digitalen Kamera dokumentiert. Diese Technik hat sich soweit verbessert, dai die Bildqualität nahezu der einer herkömmlichen Kamera entspricht. Die in einem Computer gespeicherten Bilder können bearbeitet und über das Internet verbreitet werden. [source] Ecology of the coprophagous moth Trisyntopa neossophila Edwards (Lepidoptera: Oecophoridae)AUSTRALIAN JOURNAL OF ENTOMOLOGY, Issue 2 2009Stuart J N Cooney Abstract Trisyntopa neossophila Edwards (Lepidoptera: Oecophoridae) is an unusual moth whose breeding cycle is closely synchronised with a termite mound nesting parrot of northern Australia; the hooded parrot (Psephotus dissimilis Collet). T. neossophila is one of three coprophagous, nest dwelling moths in the genus Trisyntopa Lower 1918. True coprophagy is rare in the Lepidoptera, although some species occasionally consume faeces to gain rare nutrients. We present observations of the life cycle of T. neossophila, a moth that lays its eggs in the nest of a hooded parrot, so that larvae hatch in synchrony with the hatching of the parrot's eggs. The larvae spend their larval period in the nest and exclusively consume the excrement of the nestling parrots. When the parrot chicks fledge, the larvae move to the walls of the nest cavity to pupate, emerging the following wet season to repeat the process during the next parrot breeding season. [source] Unusual multifocal granulomatous disease caused by actinomycetous bacteria in a nestling Derbyan parrot (Psittacula derbiana)AUSTRALIAN VETERINARY JOURNAL, Issue 1-2 2009FJ Park A nestling Derbyan parrot (Psittacula derbiana) was presented with unusual subcutaneous swellings of the thigh regions, and poor growth. Histological examination revealed actinomycetous bacteria associated with multifocal systemic granulomas. The clinical and pathological findings of the case are presented, and some relevant aspects of actinomycetous bacterial infections in mammals and birds are discussed. Although granulomatous disease is encountered at times in avian species, the actinomycetous bacteria (Nocardia and Actinomyces spp.) have rarely been reported in association with multifocal granulomatous disease in birds. [source] Nesting Habitat of the Lilac-crowned Parrot in a Modified Landscape in MexicoBIOTROPICA, Issue 3 2009Tiberio C. Monterrubio-Rico ABSTRACT Parrot populations are being increasingly pressured to occupy modified or fragmented landscapes, yet little is known of the habitat requirements of most species, particularly with regard to the effects on breeding habitat. We evaluated nesting habitat of the lilac-crowned parrot Amazona finschi in the modified landscape of coastal Michoacan in Mexico. We located 90 parrot nests in 12 tree species in Michoacan, with lilac-crowned parrots presenting a narrow niche-breadth of tree species used for nesting. Considering an additional 82 nest trees recorded for lilac-crowned parrots in Jalisco, we determined a 51 percent similarity in cavity resource use by parrots in the two dry forest regions. Overall, the predominant nest tree species with 76 percent of nests were Astronium graveolens, Piranhea mexicana, Brosimum alicastrum, and Tabebuia spp., all characteristic of semi-deciduous forest. Only 8 percent of nests occurred in trees characteristic of deciduous forest. Parrots utilized large trees with canopy level cavities as nest sites, and preferred conserved semi-deciduous forest for nesting, with fewer nests than expected in deciduous forest and transformed agricultural land. Nest areas in semi-deciduous forest occurred on significantly steeper terrain, as remnant semi-deciduous forest is restricted to steep ridges and canyons. Those parrot nests in modified habitats and forest patches were located near to continuous forest, with nest trees in open agricultural land being significantly closer to continuous forest than nests in disturbed forest patches. These results demonstrate the importance of conserved semi-deciduous forest as breeding habitat for the threatened, endemic lilac-crowned parrot, making wild populations of the species vulnerable to the high rate of transformation and fragmentation of tropical dry forest. RESUMEN Las poblaciones de Psitácidos están siendo presionadas a ocupar paisajes modificados, sin embargo poco se conoce sobre los requerimientos de hábitat para la mayoría de las especies, particularmente con respecto a los efectos sobre sus hábitats de anidación. Evaluamos el hábitat de anidación del loro corona lila (Amazona finschi) en el paisaje modificado de la costa de Michoacán en México. Localizamos 90 sitios de anidación en 12 especies de árboles en Michoacan, encontrando que el loro corona lila presentó una estrecha amplitud de nicho en el uso de especies de árboles para anidar. Considerando un adicional 82 árboles-nido del loro corona lila en Jalisco, determinamos un 51% de similitud en utilización del recurso de cavidades por los loros en los dos regiones de bosque seco. Los árboles claves, con 76% de los nidos, fueron Astronium graveolens, Piranhea mexicana, Brosimum alicastrum, y Tabebuia spp., todos característicos del bosque subcaducifolio. Solo 8% de los nidos se encontraron en arboles características del bosque caducifolio. Los loros utilizaron árboles grandes con cavidades en el dosel como sitios de anidación, y prefirieron el bosque subcaducifolio conservado para anidar, con un menor número de nidos que lo esperado en el bosque caducifolio y tierras agropecuarias. Las áreas de anidación en bosque subcaducifolio ocurrieron sobre pendientes significativamente más inclinadas, ya que los remanentes de este tipo de vegetación están actualmente restringidos a pendientes inclinadas y cañadas. Aquellos nidos en hábitats modificados y parches pequeños de vegetación se localizaron cerca de áreas de bosque continuo, con los árboles-nido que ocurrieron en terrenos agropecuarios significativamente más cerca de los bosques continuos, que los que ocurrieron en fragmentos de bosques perturbados. Estos resultados destacan la importancia del bosque subcaducifolio conservado como hábitat de anidación para esta especie de loro endémico y amenazado, haciendo a sus poblaciones silvestres vulnerables al alto nivel de transformación y fragmentación del bosque tropical seco. [source] Latitudinal gradients in diversity: real patterns and random modelsECOGRAPHY, Issue 3 2001Patricia Koleff Mid-domain models have been argued to provide a default explanation for the best known spatial pattern in biodiversity, namely the latitudinal gradient in species richness. These models assume no environmental gradients, but merely a random latitudinal association between the size and placement of the geographic ranges of species. A mid-domain peak in richness is generated because when the latitudinal extents of species in a given taxonomic group are bounded to north and south, perhaps by a physical constraint such as a continental edge or perhaps by a climatic constraint such as a critical temperature or precipitation threshold, then the number of ways in which ranges can be distributed changes systematically between the bounds. In addition, such models make predictions about latitudinal variation in the latitudinal extents of the distributions of species, and in beta diversity (the spatial turnover in species identities). Here we test how well five mid-domain models predict observed latitudinal patterns of species richness, latitudinal extent and beta diversity in two groups of birds, parrots and woodpeckers, across the New World. Whilst both groups exhibit clear gradients in richness and beta diversity and the general trend in species richness is acceptably predicted (but not accurately, unless substantial empirical information is assumed), the fit of these models is uniformly poor for beta diversity and latitudinal range extent. This suggests either that, at least for these data, as presently formulated mid-domain models are too simplistic, or that in practice the mid-domain effect is not significant in determining geographical variation in diversity. [source] Effects of Male Vocal Learning on Female Behavior in the Budgerigar, Melopsittacus undulatusETHOLOGY, Issue 10 2005Arla G. Hile Parrots are unusual among birds and animals in general in the extent of their ability to learn new vocalizations throughout life and irrespective of season. The budgerigar (Melopsittacus undulatus), a small parrot that is well suited for laboratory studies, has been the subject of numerous studies investigating the neurobiology of vocal learning. To date, few studies have focused on the function of vocal imitation by parrots. Previous work from our research group has shown that vocal imitation in budgerigars is sex-biased, as males paired with females learn vocalizations from their new mates, but not vice versa. This bias led us to hypothesize that vocal learning has a reproductive function. To test this hypothesis, we conducted two experiments. In the first experiment, we tutored males so that they could produce a call similar to one shared by a group of experimental females. The experimental females were then presented with one of the tutored males and another, equally unfamiliar, male that had not been tutored. We found that the females spent a greater proportion of time in proximity of, and made more affiliative displays toward, the tutored males. In the second experiment, seven males received small bilateral brain lesions that disrupt vocal learning. These males and an equal number of control males were then released into an aviary containing females and reproductive resources. We found that lesioned and control males were equally successful in obtaining social mates, but females mated to lesioned males were more likely to engage in extra-pair activities. These experiments indicate that a male's ability to imitate a female's call can influence the sexual behavior of the female even though lack of imitation ability does not appear to influence social pairing. We hypothesize that mate choice in budgerigars has multiple stages. Upon meeting a strange male, a female quickly assesses its ability for social acquisition of calls by the presence or absence of a call type similar to its own in its repertoire. As courtship proceeds into pair formation, the female assesses the ability of male to learn more directly by the extent of the male's perfection of imitation. [source] Availability and selection of arboreal termitaria as nest-sites by Orange-fronted Parakeets Aratinga canicularis in conserved and modified landscapes in MexicoIBIS, Issue 2 2009TANIA C. SANCHEZ-MARTINEZ Few studies have investigated the use of termitaria by nesting parrots, or how this may be affected by habitat transformation. We determined selection of termitaria by nesting Orange-fronted Parakeets Aratinga canicularis, and evaluated the effect of habitat transformation on the availability of termitaria nest-sites for Parakeets in the tropical dry forest of Western Mexico. Availability of termitaria was quantified in 24 survey plots in a factorial design of six 1-ha plots in each of conserved and modified, deciduous and semi-deciduous forest. Characteristics of termitaria were determined in survey plots, as well as 21 nest-termitaria used by Parakeets, and their nearest adjacent termitarium. There was an overall density of 1.6 termitaria/ha suitable for nesting by Parakeets, which did not differ between habitats, although only 8% of apparently suitable termitaria were occupied by nesting Parakeets. However, termitaria in conserved semi-deciduous forest were significantly higher above the ground, and termitaria in conserved deciduous forest were significantly smaller in volume. In the modified landscape, termitaria were significantly lower and their volume significantly larger than in conserved landscapes. Termitaria used by nesting parrots were at a significantly greater height above the ground than the nearest adjacent termitaria. Termitarium volume did not reliably predict the likelihood of nest-site selection, although Parakeets only used termitaria between 15 and 150 l. Parakeets nesting in modified habitats used termitaria at a significantly lower height than Parakeets nesting in conserved habitat. It is unclear whether this represents a decline in nest-site quality in modified habitats, which could affect reproductive success of Parakeet populations in fragmented landscapes. [source] Globally threatened parrots: criteria, characteristics and curesINTERNATIONAL ZOO YEARBOOK, Issue 1 2000N.J. COLLAR Ninety (26%) of the world's parrot species are threatened with extinction. While this figure will vary with deteriorating circumstances, taxonomic insight and assessment of new evidence, the great majority (currently 93%) of threatened parrots are forest species, most (75 species, 83%) have populations estimated at less than 10 000 mature individuals, and many (37 species, 41%) have ranges %%20 000km2. Habitat destruction (notably the loss of nest-sites), trade, hunting and introduced species are significant threats. Indonesia, Australia, Brazil, the Philippines, Colombia and Mexico support two-thirds (60 species) of all threatened parrots. Conservation of sites with sympatric threatened parrots is required, alongside research and awareness programmes that enable site and species management. [source] An overview of captive-management programmes and regional collection planning for parrotsINTERNATIONAL ZOO YEARBOOK, Issue 1 2000R. WILKINSON Psittacines are kept widely in captivity in zoos and private aviculture, and although 130 of the c. 360 parrot species are threatened, international stud-books are only maintained for a small number of these. Regional collection planning is well advanced in Australasia, North America and Europe with high-level co-ordinated breeding programmes and regional studbooks. A few regional studbooks are also maintained by zoos in Africa, Brazil and Japan. Within Australasia most programmes are for indigenous parrots and some are linked closely to species-recovery projects involving local wildlife agencies. In Europe and North America the majority of breeding programmes and studbooks are for exotic species of larger macaws, cockatoos and amazons. Some of these breeding programmes in zoos assist field conservation through active fundraising and participation of staff in field projects. Zoos also support conservation projects for species not maintained at their institutions. The role that private aviculture may play in conservation breeding programmes is discussed. [source] Situation-dependant management of large parrots by manipulation of the social environmentINTERNATIONAL ZOO YEARBOOK, Issue 1 2000C. E. KING Further exploration of alternative methods of managing large parrots in captivity, that will allow natural behaviours to be expressed, is desirable. Examples of social behavioural management of large parrots at Rotterdam Zoo and other institutions are provided in this article. The topics discussed include group pair selection, group housing and breeding, leaving the young with the parents during successive breeding efforts, managing aggression, foster incubation and foster parenting. [source] Eucalyptus pollen grain emptying by two Australian nectarivorous psittacinesJOURNAL OF AVIAN BIOLOGY, Issue 3 2001B. D. Gartrell The relative importance of pollen as a source of protein to vertebrates is controversial. In nectarivorous psittacine birds, field studies support its importance, but an experimental study in a nectarivorous parrot showed that less than 7% of pollen grains were emptied. We investigated pollen grain emptying by two nectarivorous Australian parrots, the Swift Parrot Lathamus discolor and the Musk Lorikeet Glossopsitta concinna. We used a controlled experiment, and examined pollen located at different levels through the alimentary tract of wild L. discolor. There was significant emptying of pollen grains (x=45.4%±1.91 s.e.) by all birds in the experimental trials. There was also a progressive increase in the percentage of pollen grains emptied at different sites along the alimentary tract in wild birds (crop x=24.2%±4.44 s.e., proventriculus x=34.0%±7.29 s.e., duodenum x=54.3%±5.42 s.e. and distal intestine x=64.2%±4.68 s.e.). The percentage of pollen grains emptied by captive L. discolor in the experimental trial (x=44.1%±2.77 s.e.) was not significantly different from that found in wild L. discolor (x=40.3%±4.25 s.e.). Both species of nectarivorous parrot were able to rapidly ingest large quantities of Eucalyptus pollen and appeared to empty the pollen grains efficiently. Eucalyptus pollen appears to be an important source of protein for these birds. [source] Do Meyer's Parrots Poicephalus meyeri benefit pollination and seed dispersal of plants in the Okavango Delta, Botswana?AFRICAN JOURNAL OF ECOLOGY, Issue 3 2010Rutledge S. Boyes Abstract Recent studies of new world parrots repeatedly document, with few exceptions, that parrots are wasteful and destructive predispersal seed predators that are unlikely to contribute towards pollination and seed dispersal. Few detailed studies, however, have assessed the contribution of African parrots to forest ecology by quantifying the potential net benefit of seed and flower predation by parrots for most tree species in their diet. Due to the incidence of pollen on the heads of Meyer's Parrots when feeding on Leguminosae flowers and the dispersal of viable seeds to the ground during seed predation, we compared destruction rates, when feeding on pods, fruits and flowers, with dispersal rates of viable seeds to the ground and frequency of head contact with reproductive apparatus to estimate net benefit from Meyer's Parrot feeding activity. Meyer's Parrots were not implicated in endo- or epizoochory, but they dropped uneaten fruit pulp and seeds to the ground during feeding bouts, thus providing ripe, undamaged seeds to secondary seed dispersers. This link with forest recruitment was weak, as all tree species utilized by Meyer's Parrots either had more significant primary dispersal agents or were primarily wind-dispersed. In most cases, the negative effect of seed predation outweighed any positive effects in terms of dispersal, whereby almost three times more seeds were consumed or destroyed than were dispersed to the ground. Significantly, only Sclerocarya birrea caffra recorded marginal net dispersal benefit from utilization by Meyer's Parrots. Due to low relative resource abundance and high destruction rate, feeding activity on Berchemia discolor may be significant enough to influence its spatial distribution and abundance. Utilization of flowers of Kigelia africana and Adansonia digitata by parrots likely had a significant negative impact on pollination. Feeding on Acacia nigrescens flowers, however, was potentially advantageous to their pollination. We conclude that Poicephalus parrots are net consumers of ripe, undamaged seeds and flowers, thus having an overall negative impact on forest recruitment in subtropical Africa. Résumé Les études récentes des perroquets du nouveau monde disent toujours, à quelques exceptions près, que les perroquets sont des prédateurs qui gaspillent et détruisent les semences avant leur dispersion et qu'ils sont donc peu susceptibles de contribuer à la fertilisation et à la dispersion des graines. Cependant, peu d'études détaillées ont évalué la contribution des perroquets africains à l'écologie des forêts en quantifiant le bénéfice net potentiel de la consommation des graines et des fleurs pour la plupart des espèces d'arbres qui entrent dans leur régime alimentaire. Sachant que la tête des perroquets de Meyer se charge de pollen lorsqu'ils se nourrissent de fleurs de légumineuses et qu'ils dispersent des semences viables sur le sol lorsqu'ils en consomment, nous comparons le taux de destruction, lorsqu'ils mangent des gousses, des fruits et des fleurs, avec le taux de dispersion de semences viables sur le sol et la fréquence des contacts de leur tête avec l'appareil reproducteur des plantes, pour estimer le bénéfice net de l'alimentation du perroquet de Meyer. Ces perroquets n'étaient pas impliqués dans l'endo- ou l'épizoochorie, mais lorsqu'ils mangeaient, ils laissaient tomber de la pulpe et des graines de fruits non consommées, fournissant ainsi des semences intactes à des disperseurs secondaires. Ce rapport avec le recrutement des forêts était faible dans la mesure où toutes les semences des espèces d'arbres fréquentées par les perroquets de Meyer soit avaient des agents de dispersion primaires plus importants, soit étaient principalement dispersées par le vent. Dans la plupart des cas, l'effet négatif de la consommation des semences dépassait tout effet positif en termes de dispersion, et presque trois fois plus de graines étaient mangées ou détruites que dispersées sur le sol. Il est intéressant de noter que seul Sclerocarya birrea caffra présentait un bénéfice net de dispersion marginal dûà la fréquentation des perroquets de Meyer. En raison de la faible abondance relative de ressources et du taux de destruction élevé, la consommation de Berchemia discolor pourrait être assez significative pour influencer sa distribution spatiale et son abondance. La consommation des fleurs de Kigelia africana et d'Adansonia digitata par les perroquets avait probablement un impact négatif sur leur pollinisation. Mais la consommation des fleurs d'Acacia nigrescensétait peut-être avantageuse pour la pollinisation. Nous concluons que les perroquets Poicephalus sont des consommateurs nets de graines et de fleurs mûres et intactes, et qu'ils ont donc un impact global négatif sur le recrutement des forêts en Afrique subtropicale. [source] Seasonal occurrence and local movements of the grey-headed (brown-necked) parrot Poicephalus fuscicollis suahelicus in southern AfricaAFRICAN JOURNAL OF ECOLOGY, Issue 4 2003Craig T. Symes Abstract Seasonal movements of grey-headed (brown-necked) parrots were recorded in parts of its range and are likely a response to breeding and availability of specific food sources. Breeding occurred in the northern Kruger National Park and lowveld near the Mutale,Luvhuvhu river confluence from April to August. Aggregations and movements of birds occurred during the post-breeding season (August,December) in response to seasonally abundant food sources. In north-eastern South Africa, grey-headed parrots occurred at Levubu, following the breeding season and their arrival in the area was correlated with the availability of unripe Mabola Plum, Parinari curatellifolia fruit. Similar regional movements occurred in Zimbabwe, the Caprivi of northern Namibia and Zambia. During these movements, flocks of up to 50 individuals were observed, whilst during breeding months singletons and pairs were more frequently seen. This increased abundance in time and space suggests that seasonal migratory movements occur. Résumé Des déplacements saisonniers de perroquets Poicephalus robustus ont été relevés dans des parties de leur aire de répartition, et ils sont vraisemblablement une réponse aux besoins de la reproduction et à la disponibilité des sources de leur nourriture spécifique. La reproduction avait lieu d'avril à août dans le nord du Parc National Kruger et dans le lowveld près du confluent des rivières Mutale et Luvhuvhu. Des rassemblements et des déplacements d'oiseaux avaient lieu dans la saison qui suivait celle de la reproduction (août , décembre) en réponse à l'abondance saisonnière des sources de nourriture. Au nord-est de l'Afrique du Sud, ces perroquets se rencontraient à Levubu, après la saison de reproduction, et leur arrivée coïncidait à la disponibilité des prunes Mabola (Parinari curatellifolia) avant leur maturité. On constatait des déplacements régionaux saisonniers semblables au Zimbabwe, dans le Capríví au nord de la Namibie et en Zambie. Au cours de ces déplacements, on a observé des bandes qui peuvent compter jusqu'à 50 individus, alors que pendant les mois de reproduction, on voyait plus souvent des solitaires ou des couples. Cette abondance accrue à certains moments et à certains endroits suggère qu'il existe des migrations saisonnières. [source] Molted feathers from clay licks in Peru provide DNA for three large macaws (Ara ararauna, A. chloropterus, and A. macao)JOURNAL OF FIELD ORNITHOLOGY, Issue 2 2009Kara J. Gebhardt ABSTRACT Conservation genetic analyses of wildlife have increased greatly in the past 10 yr, yet genetic studies of parrots are rare because of difficulties associated with capturing them and obtaining samples. Recent studies have demonstrated that molted feathers can provide a useful source of DNA, but success rates have varied considerably among studies. Our objective was to determine if molted macaw feathers from Blue-and-yellow Macaws (Ara ararauna), Scarlet Macaws (A. macao), and Red-and-green Macaws (A. chloropterus) collected from rainforest geophagy sites called clay licks could provide a good source of DNA for population genetic studies. Specific objectives were to determine (1) how nuclear DNA microsatellite amplification success and genotyping error rates for plucked macaw feathers compared to those for molted feathers collected from clay licks in the Amazon rainforest, and (2) if feather size, feather condition, species, or extraction method affected microsatellite amplification success or genotyping error rates from molted feathers. Amplification success and error rates were calculated using duplicate analyses of four microsatellite loci. We found that plucked feathers were an excellent source of DNA, with significantly higher success rates (P < 0.0001) and lower error rates (P= 0.0002) than for molted feathers. However, relatively high success rates (75.6%) were obtained for molted feathers, with a genotyping error rate of 11.7%. For molted feathers, we had higher success rates and lower error rates for large feathers than small feathers and for feathers in good condition than feathers that were moldy and broken when collected. We also found that longer incubation times and lower elution volumes yielded the highest quality DNA when extracting with the Qiagen DNeasy tissue kit. Our study demonstrates that molted feathers can be a valuable source of genetic material even in the challenging conditions of tropical rainforests, and our results provide valuable information for maximizing DNA amplification success rates when working with shed feathers of parrots. SINOPSIS Los análisis genéticos para la conservación de la vida silvestre han crecido en gran escala durante los últimos 10 años, pero el análisis genético de los loros son raros por las dificultades asociados con su captura y obtención de muestras. Estudios recientes han demostrado que plumas mudadas podrían proveer una fuente útil de ADN, pero las tasas de éxito varían considerablemente entre estudios. Nuestro objetivo fue determinar si las plumas mudadas de Ara ararauna, A. macao y A. chloropterus colectadas en sitios de bosque húmedo donde estas aves consumen el suelo, llamados colpas, podrían proveer una fuente útil de ADN para estudios de la genética de las poblaciones. Los objetivos específicos fueron determinar (1) como comparan las tasas de éxito de la amplificación de los microsatélites del ADN nuclear y las tasas de error en el análisis del genotipo de plumas, entre plumas colectadas directamente de los guacamayos y plumas colectadas en colpas en el bosque Amazónico, y (2) si el tamaño de la pluma, su condición, la especie o el método de extracción afecta el éxito de la amplificación de los microsatélites o las tasas de error en el análisis del genotipo de las plumas mudadas. Las tasas de éxito de amplificación y error fueron calculados usando análisis duplicados de cuatro loci de microsatélites. Encontramos que plumas colectadas directamente de las aves son una fuente excelente de ADN, con tasas de éxito significativamente más altas (P < 0.0001), y con menores tasas de error (P= 0.0002) que las plumas mudadas. Sin embargo, tasas de éxito relativamente altas (75.6%) fueron obtenidos de plumas mudadas, con una tasa de error en el análisis del genotipo de 11.7%. Para plumas mudadas, tuvimos tasas de éxito más altas y tasas de error menores para plumas grandes que para plumas pequeñas y para plumas en buena condición que para plumas que estaban cubiertos con hongos y quebradas cuando fueron colectadas. También encontramos que mayores periodos de incubación y menores volúmenes de elución proveían el ADN de mayor calidad cuando se extraía el ADN usando el kit de tejido Quiagen DNeasy. Nuestro estudio demuestra que las plumas mudadas pueden ser una fuente valiosa de materia genética, hasta en las condiciones de los bosques húmedos tropicales. Nuestros resultados proveen información valiosa para maximizar las tasas de éxito de la amplificación del ADN cuando se analizan las plumas mudadas de los loros. [source] |