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Other Consisting (other + consisting)
Selected AbstractsDetermination of 1-methylhistidine and 3-methylhistidine by capillary and chip electrophoresis with contactless conductivity detectionELECTROPHORESIS, Issue 13 2007Petr T, ma Dr. Abstract CE with capacitively coupled contactless detection (C4D) was used to determine 3-methylhistidine (3-MH) and 1-methylhistidine (1-MH). The C4D response to 3-MH was studied in a BGE consisting of 500,mM acetic acid and ammonia at varying concentration and the results were compared with the theory. Complete separation of a model mixture of 3-MH, 1-MH, and histidine (His) was attained in two optimized BGEs, one containing 500,mM HAc, 20,mM NH4OH, and 0.1 % m/v hydroxyethylcellulose (HEC), pH,3.4 (I) and the other consisting of 100,mM morpholinoethanesulfonic acid (MES), 25,mM LiOH, and 0.1 % m/v HEC, pH,5.5 (II). These optimized BGEs were tested in CE/C4D analyses of urine. Promising results were obtained for separation and determination of 3-MH, 1-MH, and His on a silicon microchip, using aluminum strips as the C4D electrodes; the three analytes were baseline-separated within less than 30,s with a separation channel effective length of 38,mm. The LOD were satisfactory and amounted to 26.4,,M for 3-MH and 18.3,,M for 1-MH. [source] Host-related life history traits in interspecific hybrids of cactophilic DrosophilaENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, Issue 1 2008E. M. Soto Abstract In the genus Drosophila (Diptera: Drosophilidae), interspecific hybridization is a rare phenomenon. However, recent evidence suggests a certain degree of introgression between the cactophilic siblings Drosophila buzzatii Patterson & Wheeler and Drosophila koepferae Fontdevila & Wasserman. In this article, we analyzed larval viability and developmental time of hybrids between males of D. buzzatii and females of D. koepferae, raised in media prepared with fermenting tissues of natural host plants that these species utilize in nature as breeding sites. In all cases, developmental time and larval viability in hybrids was not significantly different from parental lines and, depending on the cross, hybrids developed faster than both parental species or than the slowest species. When data of wing length were included in a discriminant function analysis, we observed that both species can be clearly differentiated, while hybrids fell in two categories, one intermediate between parental species and the other consisting of extreme phenotypes. Thus, our results point out that hybrid fitness, as measured by developmental time and viability, is not lower than in the parental species. [source] Measuring fatigue in patients with Parkinson's disease , the Fatigue Severity ScaleEUROPEAN JOURNAL OF NEUROLOGY, Issue 6 2002K. Herlofson The objective was to compare the prevalence and severity of fatigue in patients with Parkinson's disease (PD) with that in two control groups, one consisting of randomly chosen control subjects of the same age and sex distribution and the other consisting of patients with coxarthrosis waiting to receive total hip replacement. We also explored the possible correlation of demographic and clinical data to the presence and severity of fatigue. Sixty-six patients with PD, 131 randomly chosen controls and 79 patients with coxarthrosis, waiting to receive total hip replacement, were evaluated for fatigue. Patients and controls with a depressive mood disorder or cognitive impairment had been excluded from the study. Fatigue was measured by the Fatigue Severity Scale (FSS). For the patients with PD the mean total FSS score was 4.1, compared with 2.7 amongst the randomly chosen control group and 2.9 in the group consisting of patients with coxarthrosis. Fifty per cent of the patients with PD had a mean total FSS score of 4 or higher, compared with 25% in both of the two control groups. There was no correlation between pain, presence of self-reported nocturnal sleep disorders or duration of PD and fatigue. The patients with fatigue did have a more advanced disease, measured both by Unified Parkinson's Disease Rating Scale score and Hoehn and Yahr stage. Although the univariate analyses indicated that more severe parkinsonism was correlated to the symptom, the multivariate analysis showed that none of the studied variables were significant explanatory factors for fatigue. Fatigue is a common symptom in patients with PD without depression or dementia. The study indicates that fatigue is an independent symptom of the disease without relation to other motor or non-motor symptoms. [source] Long-memory dynamic Tobit modelsJOURNAL OF FORECASTING, Issue 5 2006A. E. Brockwell Abstract We introduce a long-memory dynamic Tobit model, defining it as a censored version of a fractionally integrated Gaussian ARMA model, which may include seasonal components and/or additional regression variables. Parameter estimation for such a model using standard techniques is typically infeasible, since the model is not Markovian, cannot be expressed in a finite-dimensional state-space form, and includes censored observations. Furthermore, the long-memory property renders a standard Gibbs sampling scheme impractical. Therefore we introduce a new Markov chain Monte Carlo sampling scheme, which is orders of magnitude more efficient than the standard Gibbs sampler. The method is inherently capable of handling missing observations. In case studies, the model is fit to two time series: one consisting of volumes of requests to a hard disk over time, and the other consisting of hourly rainfall measurements in Edinburgh over a 2-year period. The resulting posterior distributions for the fractional differencing parameter demonstrate, for these two time series, the importance of the long-memory structure in the models.,,Copyright © 2006 John Wiley & Sons, Ltd. [source] O -Glycosylated 24,kDa human growth hormone has a mucin-like biantennary disialylated tetrasaccharide attached at Thr-60PROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 13 2009Juan J. Bustamante Abstract MS was used to characterize the 24,kDa human growth hormone (hGH) glycoprotein isoform and determine the locus of O -linked oligosaccharide attachment, the oligosaccharide branching topology, and the monosaccharide sequence. MALDI-TOF/MS and ESI-MS/MS analyses of glycosylated 24,kDa hGH tryptic peptides showed that this hGH isoform is a product of the hGH normal gene. Analysis of the glycoprotein hydrolysate by high-performance anion-exchange chromatography with pulsed amperometric detection and HPLC with fluorescent detection for N -acetyl neuraminic acid (NeuAc) yielded the oligosaccharide composition (NeuAc2, N -acetyl galactosamine1, Gal1). After ,-elimination to release the oligosaccharide from glycosylated 24,kDa hGH, collision-induced dissociation of tryptic glycopeptide T6 indicated that there had been an O -linked oligosaccharide attached to Thr-60. The sequence and branching structure of the oligosaccharide were determined by ESI-MS/MS analysis of tryptic glycopeptide T6. The mucin-like O -oligosaccharide sequence linked to Thr-60 begins with N -acetyl galactosamine and branches in a bifurcated topology with one appendage consisting of galactose followed by NeuAc and the other consisting of a single NeuAc. The oligosaccharide moiety lies in the high-affinity binding site 1 structural epitope of hGH that interfaces with both the growth hormone and the prolactin receptors and is predicted to sterically affect receptor interactions and alter the biological actions of hGH. [source] | ||||||||||