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Oryza Sativa L. (oryza + sativa_l)
Selected AbstractsMODELING VARIETAL EFFECT ON THE WATER UPTAKE BEHAVIOR OF MILLED RICE (ORYZA SATIVA L.) DURING SOAKINGJOURNAL OF FOOD PROCESS ENGINEERING, Issue 6 2007B.K. YADAV ABSTRACT Milled rice is soaked until saturation before cooking and other processing. The soaking behavior of the milled rice is affected by varietal factor as well as initial moisture content (M0) of the samples. In the present study, tests were performed for milled whole kernels of 10 rice varieties ranging from low to high amylose content (16,29% d.b.) with three initial moisture levels (approximately 8, 12 and 16% d.b.) for monitoring water uptake in rice kernels during soaking at room temperature (25 ± 1C), in relation to the varietal differences manifested by the physicochemical properties. The water uptake by milled rice kernels took place at a faster rate in the beginning and was followed by a diminishing rate finally leading to a saturated value during soaking. The water uptake of the kernels during soaking could be best expressed by a modified exponential relationship with R2 values ranging from 0.971 to 0.998 for all varieties. The slope of the fitted straight line between actual and estimated moisture contents of milled rice during soaking using a modified exponential relationship was about unity (0.998) with a high R2 value of 0.989 and a root mean square error of 1.2% d.b. The parameters of the fitted model were the function of the M0 and the physicochemical properties of the milled rice. Using developed relationship, the water uptake of the milled rice during soaking could be estimated from its M0 and the physicochemical properties within±10% of the actual values. PRACTICAL APPLICATIONS This information would be useful for the scientific world working on the soaking characteristics of various varieties of rice, mainly for the modeling of the soaking process. It could also be used as a tool in selecting the rice varieties to meet their desired water uptake properties in relation to their psychochemical properties by rice breeder scientists. [source] Effect of bacteria-mineral water produced from bio-reacted fowl dung on seed germination of wheat (Triticum aestivum) and rice (Oryza sativa L.)ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY, Issue 1 2008Wenyuan He Abstract Positive effects of Bacteria-mineral water (BMW) produced from bio-reacted manure on plant growth and crop seed germination has been observed in agriculture practices. The experiment was conducted to examine the effects of BMW produced from bio-reacted fowl dung on seed germination of rice (Oryza sativa L.) and wheat (Triticum aestivum). Seeds were soaked in BMW at concentrations of 100, 10, 1, 0.5, 0.25, 0.125, 0.025, and 0% (control) and then incubated at 25°C ± 1°C in a seed germinator for 7 days. All BMW treatments not only enhanced germination energy and final germination percentage of wheat and rice seeds, but also significantly improved (P < 0.05) seed vigor index (VI). Compared to control, treatment with 0.25% BMW had significant effects (P < 0.05) on final germination percentage and increased significantly seed germination percentage (7.34%) and germination energy (8.67%) of wheat seeds. There were strong correlations between germination energy and final germination percentage (P < 0.05), germination index (P < 0.05), VI (P < 0.05), water absorption rate (P < 0.01), and storage reserve transform rate (P < 0.01). While for rice seeds, 0.25% and 0.125% BMW treatments significantly improved (P < 0.05) final germination percentage by 8% separately, and germination energy enhanced 8.66% and 9.33% respectively. There were strong correlations between germination energy (and final germination percentage) and other parameters except for water adsorption rate and storage reserve loss rate. BMW consistently showed positive effects on crop seed germination. 0.25% BMW treatment may be the best concentration to stimulate wheat (Triticum aestivum) seeds germination, while 0.125,0.25% BMW would be the most suitable concentration range for rice (Oryza sativa L.) seeds. © 2008 American Institute of Chemical Engineers Environ Prog, 2008 [source] Toward detoxifying mercury-polluted aquatic sediments with rice genetically engineered for mercury resistanceENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 12 2003Andrew C. P. Heaton Abstract Mercury contamination of soil and water is a serious problem at many sites in the United States and throughout the world. Plant species expressing the bacterial mercuric reductase gene, merA, convert ionic mercury, Hg(II), from growth substrates to the less toxic metallic mercury, Hg(0). This activity confers mercury resistance to plants and removes mercury from the plant and substrates through volatilization. Our goal is to develop plants that intercept and remove Hg(II) from polluted aquatic systems before it can undergo bacterially mediated methylation to the neurotoxic methylmercury. Therefore, the merA gene under the control of a monocot promoter was introduced into Oryza sativa L. (rice) by particle gun bombardment. This is the first monocot and first wetland-adapted species to express the gene. The merA -expressing rice germinated and grew on semisolid growth medium spiked with sufficient Hg(II) to kill the nonengineered (wild-type) controls. To confirm that the resistance mechanism was the conversion of Hg(II) to Hg(0), seedlings of merA -expressing O. sativa were grown in Hg(II)-spiked liquid medium or water-saturated soil media and were shown to volatilize significantly more Hg(0) than wild-type counterparts. Further genetic manipulation could yield plants with increased efficiency to extract soil Hg(II) and volatilize it as Hg(0) or with the novel ability to directly convert methylmercury to Hg(0). [source] Seasonal changes in the effects of elevated CO2 on rice at three levels of nitrogen supply: a free air CO2 enrichment (FACE) experimentGLOBAL CHANGE BIOLOGY, Issue 6 2003HAN-YONG KIM Abstract Over time, the stimulative effect of elevated CO2 on the photosynthesis of rice crops is likely to be reduced with increasing duration of CO2 exposure, but the resultant effects on crop productivity remain unclear. To investigate seasonal changes in the effect of elevated CO2 on the growth of rice (Oryza sativa L.) crops, a free air CO2 enrichment (FACE) experiment was conducted at Shizukuishi, Iwate, Japan in 1998,2000. The target CO2 concentration of the FACE plots was 200 µmol mol,1 above that of ambient. Three levels of nitrogen (N) were supplied: low (LN, 4 g N m,2), medium [MN, 8 (1998) and 9 (1999, 2000) g N m,2] and high N (HN, 12 and 15 g N m,2). For MN and HN but not for LN, elevated CO2 increased tiller number at panicle initiation (PI) but this positive response decreased with crop development. As a result, the response of green leaf area index (GLAI) to elevated CO2 greatly varied with development, showing positive responses during vegetative stages and negative responses after PI. Elevated CO2 decreased leaf N concentration over the season, except during early stage of development. For MN crops, total biomass increased with elevated CO2, but the response declined linearly with development, with average increases of 32, 28, 21, 15 and 12% at tillering, PI, anthesis, mid-ripening and grain maturity, respectively. This decline is likely to be due to decreases in the positive effects of elevated CO2 on canopy photosynthesis because of reductions in both GLAI and leaf N. Up to PI, LN-crops tended to have a lower response to elevated CO2 than MN- and HN-crops, though by final harvest the total biomass response was similar for all N levels. For MN- and HN-crops, the positive response of grain yield (ca. 15%) to elevated CO2 was slightly greater than the response of final total biomass while for LN-crops it was less. We conclude that most of the seasonal changes in crop response to elevated CO2 are directly or indirectly associated with N uptake. [source] Contrasting infection frequencies of Neotyphodium endophyte in naturalized Italian ryegrass populations in Japanese farmlandsGRASSLAND SCIENCE, Issue 2 2010Masayuki Yamashita Abstract Neotyphodium endophytes often confer benefits to their host grasses and may enhance invasiveness of some grasses. The knowledge of infection frequencies of endophytes among invading weed populations is necessary to understand the relationships between endophyte infection and invasiveness. Here we present data on infection frequencies of Italian ryegrass (Lolium multiflorum Lam.), an important weed in some farmlands in Japan, persisting in contrasting farmlands: a terraced paddy field and a wheat-soybean double-cropped field in the western region of Shizuoka prefecture, Japan. The terraced paddy site is a mosaic of several landscape elements such as paddy fields, levees, fallow and abandoned fields, with a high percentage of non-crop area. Rice (Oryza sativa L.) has been cultivated for more than a decade with no application of chemical fertilizers, pesticides and fungicides. The wheat-soybean field is characterized by the aggregation of large-scaled fields that were originally reconstructed paddy fields, showing a low percentage of non-crop area. Wheat and soybean have been grown as winter and summer crops, respectively, using chemical fertilizers and herbicides. We examined the presence or absence of endophytes in a total of 1200 seeds sampled from the two Italian ryegrass populations. The terraced paddy population exhibited a markedly high infection frequency (91.0%), due possibly to selective feeding of non-infected seeds by insects. In contrast, the wheat-soybean farmland population showed almost no infection (1.1%), whereas the putative source of the invasion in the proximity exhibited a relatively high infection rate (64.4%). Such a micro-scale variation in infection frequencies may be attributable to a loss in endophyte viability within the wheat-soybean field. The findings suggest that endophyte infection frequency may markedly differ among the Italian ryegrass populations even within the same region, presumably depending on the abundance of the seed-eating insects, farmland management regimes and/or environmental conditions such as soil humidity. [source] Ensilage of wilted whole crop rice (Oryza sativa L.) using a roll baler for chopped material: Silage quality in long-term storageGRASSLAND SCIENCE, Issue 2 2007Hidenori Kawamoto Abstract We examined the effects of long-term storage on the fermentation quality, chemical composition, and digestibility of wilted whole crop rice silage prepared using a roll baler for chopped material (set chop length, 13 mm) and compared the results with those obtained by using a conventional roll baler. The roll balers were used for ensiling whole crop rice of three types: (i) dough-ripe stage with light wilting (45% dry matter); (ii) dough-ripe stage with heavy wilting (65% dry matter); and (iii) yellow-ripe stage with light wilting (45% dry matter). The apparent dry matter density was higher in the ensiled roll bales composed of chopped whole crop rice (chopped bales) than in those composed of non-chopped whole crop rice (conventional bales) (195,250 kg m,3 vs 156,218 kg m,3, respectively). The formation of volatile fatty acids and ammonia-nitrogen was low in all types of silage. Further, no marked differences in the chemical composition and apparent dry matter digestibility were observed between silage from the two types of bales. However, there were significant differences in their lactic acid and ethanol contents. The lactic acid production in the conventional silage was low (0.08,0.14% fresh matter), whereas that in the chopped silage was high (0.71,0.97% fresh matter). A lower pH value (pH 4.0,4.3) was retained in the chopped silage after 10 months of storage. High ethanol production (1.1,2.5% fresh matter) was observed in the conventional silage, whereas ethanol production decreased to less than 1% in the chopped silage. These results indicate that although the ethanol fermentation is readily enhanced in the ensilage of wilted whole crop rice performed by a conventional baler, the ensilage performed by a baler for chopped material encourages lactic acid fermentation and suppresses ethanol production over a wide moisture range throughout the long-term storage. [source] DROUGHT STRESS: Comparative Time Course Action of the Foliar Applied Glycinebetaine, Salicylic Acid, Nitrous Oxide, Brassinosteroids and Spermine in Improving Drought Resistance of RiceJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 5 2010M. Farooq Abstract Worldwide rice productivity is being threatened by increased endeavours of drought stress. Among the visible symptoms of drought stress, hampered water relations and disrupted cellular membrane functions are the most important. Exogenous use of polyamines (PAs), salicylic acid (SA), brassinosteroids (BRs), glycinebetaine (GB) and nitrous oxide (NO) can induce abiotic stresses tolerance in many crops. In this time course study, we appraised the comparative role of all these substances to improve the drought tolerance in rice (Oryza sativa L.) cultivar Super-Basmati. Plants were subjected to drought stress at four leaf stage (4 weeks after emergence) by maintaining soil moisture at 50 % of field capacity. Pre-optimized concentrations of GB (150 mg l,1), SA (100 mg l,1), NO (100 ,mol l,1 sodium nitroprusside as NO donor), BR (0.01 ,m 24-epibrassinolide) and spermine (Spm; 10 ,m) were foliar sprayed at five-leaf stage (5 weeks after emergence). There were two controls both receiving no foliar spray, viz. well watered (CK1) and drought stressed (CK2). There was substantial reduction in allometric response of rice, gas exchange and water relation attributes by drought stress. While drought stress enhanced the H2O2, malondialdehyde (MDA) and relative membrane permeability, foliar spray of all the chemicals improved growth possibly because of the improved carbon assimilation, enhanced synthesis of metabolites and maintenance of tissue water status. Simultaneous reduction in H2O2 and MDA production was also noted in the plants treated with these substances. Drought tolerance was sturdily associated with the greater tissue water potential, increased synthesis of metabolites and enhanced capacity of antioxidant system. Of all the chemicals, foliar spray with Spm was the most effective followed by BR. [source] Exogenously Applied Nitric Oxide Enhances the Drought Tolerance in Fine Grain Aromatic Rice (Oryza sativa L.)JOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 4 2009M. Farooq Abstract Drought stress is a severe threat to the sustainable rice production, which causes oxidative damage and disturbs plant water relations, while exogenously applied nitric oxide (NO) may have the potential to alleviate these effects in rice plants. In this study, the role of NO to improve drought tolerance in fine grain aromatic rice (Oryza sativa L. cv. Basmati 2000) was evaluated. Sodium nitroprusside, a NO donor, was used at 50, 100 and 150 ,mol l,1 both as seed priming and foliar spray. To prime, the seeds were soaked in aerated NO solution of respective solution for 48 h and dried back to original weight. Primed and non-primed seeds were sown in plastic pots with normal irrigation in a greenhouse. At four leaf stage, plants were subjected to drought stress except the controls, which were kept at full field capacity. Drought was maintained at 50 % of field capacity by watering when needed. Two controls were maintained; both receiving no NO treatments as foliar application or seed treatment, one under drought conditions and the other under well-watered conditions. Drought stress seriously reduced the rice growth, but both methods of NO application alleviated the stress effects. Drought tolerance in rice was strongly related to the maintenance of tissue water potential and enhanced capacity of antioxidants, improved stability of cellular membranes and enhanced photosynthetic capacity, plausibly by signalling action of NO. Foliar treatments proved more effective than the seed treatments. Among NO treatment, 100 ,mol l,1 foliar spray was more effective. [source] Water Deficit Reduced Fertility of Young Microspores Resulting in a Decline of Viable Mature Pollen and Grain Set in RiceJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 1 2009G. N. Nguyen Abstract Pollen formation in rice (Oryza sativa L.) is highly vulnerable to environmental stresses such as heat, chilling and drought. In rice plants exposed to drought during male reproductive development, the most obvious damage often observed is a decline in the number of engorged pollen and grain set. This has been well characterized in rice under chilling and to a lesser extent under drought stress. Moreover, detailed literature on the immediate effects of drought on developing young microspores in rice is still limited. Here, we report findings from experiments on rice plants exposed to water deficit for three consecutive days during early stages of anther development. When the osmotic potential of the growing medium was equal to or less than ,0.5 MPa, as induced by polyethylene glycol, the leaf water potential was significantly lowered and grain set was reduced. A strong correlation between grain set and viable young microspores (P < 0.001, r2 = 0.8223) indicates that water deficit immediately reduced fertility of rice plants at the time of exposure. This result suggests a new underlying mechanism of water deficit-induced pollen abortion in rice. [source] Effect of Straw on Yield Components of Rice (Oryza sativa L.) Under Rice-Rice Cropping SystemJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 2 2006K. Surekha Abstract Field experiments were conducted at the Directorate of Rice Research experimental farm, ICRISAT campus, Patancheru, Hyderabad, during 1998,2000 for five consecutive seasons (three wet and two dry seasons) with five treatments [T1 , 100 % straw incorporation; T2 , 50 % straw incorporation; T3 , 100 % straw + green manure (GM) incorporation; T4 , 100 % straw burning and T5 , 100 % straw removal (control)] along with the recommended dose of fertilizers to evaluate the effect of different crop residue management (CRM) practices on yield components and yield of rice in rice,rice cropping sequence. The ammonium N measured at active tillering was higher in 100 % straw-added plots over 50 % straw addition and straw removal with maximum values in the straw + GM-incorporated plots. Among the yield components, tillers, panicles and spikelets were influenced from the second season of residue incorporation with significant increase in 100 % straw-added treatments. The increase in tiller and panicle number could be attributed to the increased NH4 -N in these treatments, which is evident from the significant correlation between tiller number and NH4 -N (r = 0.82**) and panicle number and NH4 -N (r = 0.87**). The influence of residue treatments on rice grain yield was observed from the third season onwards where incorporation of straw alone or in combination with GM and burning of straw significantly increased grain and straw yields. Grain yield showed significant positive correlation with the number of tillers (r = 0.74*,0.81**) and panicles (r = 0.74*,0.84**) in three treatments (T1, T3 andT4) where grain yields were significantly higher. The regression analysis showed that 57,66 % and 64,75 % of the variation in yield could be explained by tillers and panicles together in these three treatments during wet and dry seasons respectively. Thus, CRM practices such as addition of 100 % straw either alone or with GM and straw burning influenced the yield components (tillers, panicles and spikelets) positively and thereby increased rice grain yields. [source] Inter-relationships Amongst Grain Characteristics, Grain-Filling Parameters and Rice (Oryza sativa L.) Milling QualityJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 4 2001S. Jongkaewwattana Resistance to breakage is a desirable trait of the rice kernel. Many factors, such as the genetics of the cultivar, the plant growth environment and the conditions of the milling process, will affect kernel breakage. Although many papers have discussed the factors that may affect and improve rice milling quality, few have related the grain-filling process to head rice, the unbroken polished kernels obtained after milling. The objectives of this paper were: (i) to characterize the interrelationships amongst grain filling and grain structural characteristics; (ii) to determine whether the grain-filling process and grain characteristics affect head rice, and (iii) to suggest a pathway through which grain characteristics can influence head rice recovery. An analysis of the interrelationships amongst all grain characteristics suggested that variables of grain structure (size, volume and per cent hull) have a decisive influence on the grain-filling process (rate and duration of grain filling). The grain-filling process will affect final grain traits such as weight and density, which in turn will have a direct impact on head rice. In addition, non-uniformity, whether expressed in terms of variable grain size and shape or grain filling and maturity, has a detrimental effect on rice milling quality. The implication of these findings is that rice breeders need to pay more attention to selecting plant types that have a high degree of uniformity of grain characteristics on the panicle, and to those traits (such as greater grain size, weight and density) that have a positive impact on yield and milling quality. Beziehungen zwischen Korneigenschaften, Kornfüllungsparametern und Reis (Oryza sativa L.)-Vermahlungsqualität Bruchresistenz von Reiskörnern ist eine wünschenswerte Eigenschaft. Viele Faktoren, wie Genetik, Umwelt des Pfanzenwachstums und Voraussetzungen der Vermahlung, beeinflussen die Bruchresistenz der Körner. Obwohl viele Veröffentlichungen die Faktoren diskutieren, die die Mahlqualität beinträchtigen oder verbessern, beziehen sich nur wenige auf den Kornfüllungsvorgang zu ungebrochenen polierten Körner nach dem Mahlvorgang. Ziel der Untersuchungen war es: zu charakterisieren die Beziehungen zwischen Kornfüllung und Kornstruktur; zu bestimmen, ob der Kornfüllungsprozess und Korneigenschaften die Bruchresistenz von Reis beeinflussen können; und vorzuschlagen eine Behandlung durch die Korneigenschaften die Wiedergewinnung von Bruchreis beieinflußt werden kann. Eine Analyse der Beziehungen zwischen den Korneigenschaften weist darauf hin, dass Variable der Kornstruktur (Größe, Umfang und Antiel der Schale) einen deutlichen Einfluß auf den Kornfüllungsprozess haben (Rate und Dauer der Kornfüllung). Der Kornfüllungsprozess beeinflußt Korngewicht und Korndichte, die eine unmittelbare Auswirkung auf ungebrochene Körner nach dem Mahlvorgang haben. Auch Uneinheitlichkeit in Korngröße und Kornform oder Kornfüllung und Kornreife haben einen ungünstigen Einfluß auf die Vermahlungsqualität von Reis. Hieraus ergibt sich der Hinweis, daß Reiszüchter in der Selketion auf einen hohen Grad von Einheitlichkeit der Körner der Rispe und deren Eigenschaften (hohe Korngröße, Korngewicht und Korndichte), die einen Einfluß auf Ertrag und Mahlqualität haben, achten sollten. [source] Olfactory response of Trigonotylus caelestialium (Het.: Miridae) to rice plant and gramineous weedsJOURNAL OF APPLIED ENTOMOLOGY, Issue 8 2007T. Niiyama Abstract:, The olfactory response of Trigonotylus caelestialium, to rice, Oryza sativa L., and two species of gramineous weeds, Poa annua and Digitaria ciliaris, was investigated with an olfactometer to clarify the role of host-plant odours as olfactory cues in their host-finding behaviour. Third-instar nymphs and adult males were significantly attracted to whole plants (above ground parts) of P. annua. However, adult females were not significantly attracted to whole plants of P. annua. Attractancy of rice to T. caelestialium differed with the growth stage and part of the plant. Adult females were significantly attracted to stems and leaves in the panicle-formation stage, and panicles in the flowering stage. They were not attracted to stems and leaves in the fourth-leaf stage and flowering stage, or to panicles in the milk-and full-ripe stages. Although adult males were significantly attracted to stems and leaves in the panicle-formation stage, they were not attracted to the other rice structures tested. Both females and males were significantly attracted to stems and leaves, as well as panicles of D. ciliaris in the flowering stage. The findings suggest that T. caelestialium use host-plant volatiles as olfactory cues to find their host plants and their invasion to paddy fields were caused by olfactory responses to rice plant. [source] Influence of host plant odours on invasion of the rice leaf bug Trigonotylus caelestialium into paddy fieldsAGRICULTURAL AND FOREST ENTOMOLOGY, Issue 1 2010Tatsuya Fujii 1The host-odour preferences of the rice leaf bug Trigonotylus caelestialium between the rice plant Oryza sativa L. and four species of graminaceous weeds, Poa annua, Alopecurus aequalis, Digitaria ciliaris and Eleusine indica, were investigated with an olfactometer aiming to clarify the influence of these odours on invasion of the bug to paddy fields at the flowering stage of rice. 2Both female and male adults significantly preferred the graminaceous weed A. aequalis in the flowering stage to rice in the fifth-leaf stage. The bugs also significantly preferred flowering P. annua and A. aequalis to rice in the panicle-formation stage. However, the bugs showed no preferences between rice in the flowering and grain-filling stages and the flowering graminaceous weeds P. annua, D. ciliaris and E. indica. 3The preference of the rice leaf bug for the flowering graminaceous weeds before rice flowering coincides with the fact that these bugs mainly live on these weeds before rice flowering. It is considered that the bug's similar preference for flowering rice panicles as the flowering graminaceous weeds causes the intense invasion of the bug into paddy fields at this rice developmental stage. [source] Three-Dimensional Lipid Distribution of a Brown Rice KernelJOURNAL OF FOOD SCIENCE, Issue 7 2002Y. Ogawa ABSTRACT: Lipid distribution was successfully observed in a brown rice kernel (Oryza sativa L.) 3-dimension-ally (3D) by means of a virtual 3D visualizing model. Sections of an untreated rice kernel were collected on an adhesive tape with preservation of its shape. The actual distribution of lipid was visualized by staining. A virtual 3D visualizing model of the lipid distribution was produced from the stained sequential sections of the rice kernel. Lipid is not only located at the outer layer of the rice kernel but also in lower tissues beneath the seed coat and around the embryo. Lipid distribution at dorsal and ventral sides could also be visualized. [source] Effects of Elevated CO2 on Growth, Carbon Assimilation, Photosynthate Accumulation and Related Enzymes in Rice Leaves during Sink-Source TransitionJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 6 2008Jun-Ying Li Abstract To study the effects of growing rice (Oryza sativa L.) leaves under the treatment of the short-term elevated CO2 during the period of sink-source transition, several physiological processes such as dynamic changes in photosynthesis, photosynthate accumulation, enzyme activities (sucrose phosphate synthase (SPS), and sucrose synthase (SS)), and their specific gene (sps1 and RSus1) expressions in both mature and developing leaf were measured. Rice seedlings with fully expanded sixth leaf (marked as the source leaf, L6) were kept in elevated (700 ,mol/mol) and ambient (350 mol/L) CO2 until the 7th leaf (marked as the sink leaf, L7) fully expanded. The results demonstrated that elevated CO2 significantly increased the rate of leaf elongation and biomass accumulation of L7 during the treatment without affecting the growth of L6. However, in both developing and mature leaves, net photosynthetic assimilation rate (A), all kinds of photosynthate contents such as starch, sucrose and hexose, activities of SPS and SS and transcript levels of sps1 and RSus1 were significantly increased under elevated CO2 condition. Results suggested that the elevated CO2 had facilitated photosynthate assimilation, and increased photosynthate supplies from the source leaf to the sink leaf, which accelerated the growth and sink-source transition in new developing sink leaves. The mechanisms of SPS regulation by the elevated CO2 was also discussed. [source] Cell-wall Invertases from Rice are Differentially Expressed in Caryopsis during the Grain Filling StageJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 4 2008Yong-Qin Wang Abstract Cell-wall invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To investigate the role of cell-wall invertases for seed development in rice (Oryza sativa L.), cDNAs of three putative cell-wall invertase genes OsCIN1, OsCIN2 and OsCIN3 were isolated. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed different expression patterns of the three genes in various rice tissues/organs. In developing caryopses, they exhibited similar temporal expression patterns, expressed highly at the early and middle grain filling stages and gradually declined to low levels afterward. However, the spatial expression patterns of them were very different, with OsCIN1 primarily expressed in the caryopsis coat, OsCIN2 in embryo and endosperm, and OsCIN3 in embryo. Further RNA in situ hybridization analysis revealed that a strong signal of OsCIN2 mRNA was detected in the vascular parenchyma surrounding the xylem of the chalazal vein and the aleurone layer, whereas OsCIN3 transcript was strongly detected in the vascular parenchyma surrounding the phloem of the chalazal vein, cross-cells, the aleurone layer and the nucellar tissue. These data indicate that the three cell-wall invertase genes play complementary/synergetic roles in assimilate unloading during the grain filling stage. In addition, the cell type-specific expression patterns of OsCIN3 in source leaf blades and anthers were also investigated, and its corresponding physiological roles were discussed. [source] The 6-phosphogluconate Dehydrogenase Genes Are Responsive to Abiotic Stresses in RiceJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 5 2007Fu-Yun Hou Abstract Glucose-6-phosphate dehydrogenase (G6PDH, E.C. 1.1.1.49) and 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) are both key enzymes of the pentose phosphate pathway (PPP). The OsG6PDH1 and Os6PGDH1 genes encoding cytosolic G6PDH and cytosolic 6PGDH were isolated from rice (Oryza sativa L.). We have shown that Os6PGDH1 gene was up-regulated by salt stress. Here we reported the isolation and characterization of Os6PGDH2 from rice, which encode the plastidic counterpart of 6PGDH. Genomic organization analysis indicated that OsG6PDH1 and OsG6PDH2 genes contain multiple introns, whereas two Os6PGDH1 and Os6PGDH2 genes have no introns in their translated regions. In a step towards understanding the functions of the pentose phosphate pathway in plants in response to various abiotic stresses, the expressions of four genes in the rice seedlings treated by drought, cold, high salinity and abscisic acid (ABA) were investigated. The results show that OsG6PDH1 and OsG6PDH2 are not markedly regulated by the abiotic stresses detected. However, the transcript levels of both Os6PGDH1 and Os6PGDH2 are up-regulated in rice seedlings under drought, cold, high salinity and ABA treatments. Meanwhile, the enzyme activities of G6PDH and 6PGDH in the rice seedlings treated by various abiotic stresses were investigated. Like the mRNA expression patterns, G6PDH activity remains constant but the 6PGDH increases steadily during the treatments. Taken together, we suggest that the pentose phosphate pathway may play an important role in rice responses to abiotic stresses and the second key enzyme of PPP, 6PGDH, may function as a regulator controlling the efficiency of the pathway under abiotic stresses. (Handling editor: Kang Chong) [source] Rice Mitochondrial Genes Are Transcribed by Multiple Promoters That Are Highly DivergedJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 12 2006Qun-Yu Zhang Abstract Plant mitochondrial genes are often transcribed into complex sets of mRNA. To characterize the transcription initiation and promoter structure, the transcript termini of four mitochondrial genes, atp1, atp6, cob, rps7, in rice (Oryza sativa L.), were determined by using a modified circularized RNA reverse transcription-polymerase chain reaction method. The results revealed that three genes (atp1, atp6, rps7) were transcribed from multiple initiation sites, indicating the presence of multiple promoters. Two transcription termination sites were detected in three genes (atp6, cob, rps7), respectively. Analysis on the promoter architecture showed that the YRTA (Y=T or C, R=A or G) motifs that are widely present in the mitochondrial promoters of other monocotand dicot plant species were detected only in two of the 12 analyzed promoters. Our data suggest that the promoter sequences in the rice mitochondrial genome are highly diverged in comparison to those in other plants, and the YRTA motif is not an essential element for the promoter activity. (Managing editor: Li-Hui Zhao) [source] Genotyping the Heading Date of Male-Sterile Rice Line II-32AJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 4 2006Jun-Feng Xu Abstract II-32A, an elite male-sterile line of rice (Oryza sativa L.), has been widely used for the production of hybrid rice seed in China. Heading date in most combinations using II-32A shows transgressive inheritance or similarity to the latter parent, but the genotype of II-32A with respect to major genes for heading time is unknown. This limits the further exploitation of this sterile line in breeding and hybrid seed production. Using a number of major gene heading date isogenic lines and heading date QTL near-isogenic lines, we genetically analyzed II-32B under both long- and short-day conditions. We show that II-32B carries two photoperiod-sensitive genes, E1 and E3, a recessive late-heading gene, ef-1, and a photoperiod-sensitive allele, Se-1u. In addition we identified in II-32B a recessive inhibitor for E1 or Se-1n and other modified photoperiod-sensitive genes. The heading-date constitution of II-32A was determined to be E1e2E3Se-1uef-1i-Se-1. (Managing editor: Li-Hui Zhao) [source] Genetic Analysis and Mapping of the Dominant Dwarfing Gene D-53 in RiceJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 4 2006Li-Rong Wei Abstract The dwarfing gene D-53 is one of a few dominant genes for dwarfing in rice (Oryza sativa L.). In the present study, our genetic analysis confirmed that mutant characteristics including dwarfing, profuse tillering, thin stems and small panicles are all controlled by the dominant D-53 gene. We measured the length of each internode of KL908, a D-53- carrying line, and classified the dwarfism of KL908 into the dn-type. In addition, we measured elongation of the second sheath and ,-amylase activity in the endosperm, and we characterized KL908 as a dwarf mutant that was neither gibberellic acid-deficient nor gibberellic acid-insensitive. Using a large F2 population obtained by crossing KL908 with a wild-type variety, NJ6, the D-53 gene was mapped to the terminal region of the short arm of chromosome 11, with one simple sequence repeat marker, Ds3, co-segregating, and the other, K81114, located 0.6 cM away. (Managing editor: Li-Hui Zhao) [source] Genetic Analysis of Tolerance to Rice Tungro Bacilliform Virus in Rice (Oryza sativa L.) Through AgroinoculationJOURNAL OF PHYTOPATHOLOGY, Issue 4 2006N. S. Zenna Abstract Balimau Putih [an Indonesian cultivar tolerant to rice tungro bacilliform virus (RTBV)] was crossed with IR64 (RTBV, susceptible variety) to produce the three filial generations F1, F2 and F3. Agroinoculation was used to introduce RTBV into the test plants. RTBV tolerance was based on the RTBV level in plants by analysis of coat protein using enzyme-linked immunosorbent assay. The level of RTBV in cv. Balimau Putih was significantly lower than that of IR64 and the susceptible control, Taichung Native 1. Mean RTBV levels of the F1, F2 and F3 populations were comparable with one another and with the average of the parents. Results indicate that there was no dominance and an additive gene action may control the expression of tolerance to RTBV. Tolerance based on the level of RTBV coat protein was highly heritable (0.67) as estimated using the mean values of F3 lines, suggesting that selection for tolerance to RTBV can be performed in the early selfing generations using the technique employed in this study. The RTBV level had a negative correlation with plant height, but positive relationship with disease index value. [source] Using farmer knowledge to combat low productive spots in rice fields of a Sahelian irrigation schemeLAND DEGRADATION AND DEVELOPMENT, Issue 4 2004P. J. A. van Asten Abstract In the oldest sections of Burkina Faso's largest irrigation scheme in the Sourou Valley (13°,10,,N, 03°,30,W) rice (Oryza sativa L.) yields dropped from about 5 to 6,t,ha,1 in the early 1990s, shortly after establishment of the scheme, to 2 to 4,t,ha,1 from 1995 onwards. Farmers blamed this yield decline on the appearance of 2 to 20,m diameter low productive spots. According to farmers and field measurements, the low productive spots decreased yields by 25,50 per cent. The low productive spots are caused by Zn deficiency. Low Zn availability is related to the very low DTPA-extractable Zn content of the soil (0·08,0·46,mg,kg,1), the alkaline-calcareous character of the soil, the non-application of Zn fertilizers, and a relatively large P fertilizer dose (21,kg,P,ha,1). Farmers were correct in relating the calcareous nature of the soil to the presence of the low productive spots. They were instrumental in identifying application of decomposed organic resources (e.g. rice straw at 5,t,ha,1) as a short-term solution that increases yields by 1·5 to 2·0,t,ha,1. Application of Zn fertilizer (10,kg,Zn,ha,1) in 29 farmer fields in the 2001 dry season eradicated the low productive spots and increased yields from 3·4 to 6·0,t,ha,1. Although application of Zn fertilizer is strongly recommended, it is not yet available in Burkina Faso. Based on a comparison of fertilizer prices on the world market and the local market, we expect that the use of Zn fertilizers will be highly profitable (cost/value ratio,,,2). Despite the relatively recent introduction of irrigated rice cropping, most farmers showed a good understanding of cropping constraints and possible solutions. Both farmers and researchers mutually benefited from each other's knowledge and observations. Copyright © 2004 John Wiley & Sons, Ltd. [source] Rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibitionLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 1 2003Wenli Chen Abstract Using a highly sensitive single photon counter, a spontaneous chemiluminescence (CL) study on rice (Oryza sativa L.) seeds stored in different years was carried out. We first observed that the degree of ageing in rice seeds was related to the intensity of spontaneous CL during early imbibition (0,30 min). Rice seeds stored for a shorter time had a stronger intensity of CL in early imbibition. The germination rate of rice seeds showed an obvious positive correlation with the intensity of spontaneous CL. Singlet oxygen (1O2) in rice seeds during early imbibition was investigated by a CL method using a cypridina luciferin analogue, 2-methyl-6-(p -methoxyphenyl)-3,7-dihydroimidazo [1,2,] pyrazin-3-one (MCLA), as a selective CL probe. Additional experimental evidence for the formation of 1O2 came from the quenching effect of sodium azide (NaN3) on MCLA-mediated rice seeds' CL. Analysis based on the experimental results demonstrated that spontaneous CL in rice seeds during early imbibition was mainly contributed by singlet oxygen (1O2). Copyright © 2002 John Wiley & Sons, Ltd. [source] Isovitexin-2,- O -,-[6- O -E- p -coumaroylglucopyranoside] from UV-B irradiated Leaves of Rice, Oryza sativa L. Inhibits Fertility of Helicoverpa armigeraPHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 5 2007Merdelyn T. Caasi-Lit UV-B irradiated rice leaves (Oryza sativa L.) contained four closely related flavonoids, with either an isoorientin or isovitexin aglycone. These flavonoids have previously been purified and characterized, and were added to artificial diets of the African bollworm (Helicoverpa armigera Hübner) at 0.1× concentration found in irradiated rice leaves. Consumption of different diets had relatively small effects on laval, pupal and adult duration, weight and survival, indicating the insects lived near normal life cycles on all diets. However, one of the compounds, flavonoid IIa, isovitexin-2,- O -,-[6- O -E- p -coumaroylglucopyranoside], dramatically reduced the number of fertile eggs laid to 7% of control insects (P < 0.001) when added to insect diets at 18 nmol gFW,1 (14 ppm). A similar antifertility effect was observed when only the male partner consumed diet containing flavonoid IIa, indicating that the reduced fertility may be male specific. In contrast, the fecundity and fertility of insects eating diets containing the closely related flavonoids, isoorientin-2,- O -,-[6- O -E- p -coumaroylglucopyranoside] or isoorientin-2,- O -,-[6- O -E- p -feruloylglucopyranoside], were not significantly different to control diets. [source] Effects of Crude and Partially Purified Extracts from UV-B,irradiated Rice Leaves on Helicoverpa armigera (Hübner),PHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 5 2005Merdelyn T. Caasi-Lit ABSTRACT The effect of crude and partially purified extracts from ultraviolet-B (UV-B),irradiated rice (Oryza sativa L.) leaves on the growth and development of corn earworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), was investigated. Fifty ,L droplets of a liquid diet containing different concentrations of the crude and partially purified extracts were fed to H. armigera neonates to determine possible short-term toxicity effects. A choice test using a solid artificial diet was also performed to determine larval feeding preferences and antifeedant effects. To study effects on the life history of the insect, different concentrations of the crude and partially purified extracts were also incorporated in the artificial diet and fed to individually confined neonates of H. armigera. The neonates were reared up to the adult stage. Results showed that crude and partially purified extracts of UV-B,irradiated rice leaves demonstrated antifeedant, growth-inhibitory and antibiotic properties against H. armigera. At high concentrations, the extract initially stimulated larval feeding; however, there were subsequent negative effects on pupal and adult traits, thereby reducing the reproductive potential of adults. These partially purified extracts appeared to have an antifertility effect because adults laid fewer eggs and, of those eggs laid, viability was lower. These results suggest that the accumulated flavonoids or other phenolics in UV-B,irradiated leaves, extracted from UV-B,resistant rice cultivar ,M202,' affected the growth, development and reproduction of H. armigera, a polyphagous insect pest. [source] Isolation and identification of a potent allelopathic substance in rice root exudatesPHYSIOLOGIA PLANTARUM, Issue 3 2002Hisashi Kato-Noguchi A search for growth inhibitors in rice root exudates was undertaken in order to clarify the allelopathic system in rice (Oryza sativa L.). Rice seedlings inhibited the growth of cress (Lepidium sativum L.) and lettuce (Lactuca sativa L.) seedlings when the cress and lettuce were grown with rice seedlings. The putative compound causing the inhibitory effect of rice seedlings was isolated from their culture solution, and the chemical structure of the inhibitor was determined by spectral data as momilactone B. Momilactone B inhibited the growth of cress and lettuce seedlings at concentrations greater than 3 and 30 µM, respectively. The concentration of momilactone B was 3.4 and 1.1 nmol per seedling in the culture solutions of husked and non-husked rice seedlings, respectively. These results suggest that rice seedlings may release momilactone B into the environment and the stress caused by the husk-treatment may increase the amount of momilactone B released. Thus, momilactone B may play an important role in rice allelopathy. [source] Functional analysis of rice NPR1 -like genes reveals that OsNPR1/NH1 is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility,PLANT BIOTECHNOLOGY JOURNAL, Issue 2 2007Yuexing Yuan Summary The key regulator of salicylic acid (SA)-mediated resistance, NPR1, is functionally conserved in diverse plant species, including rice (Oryza sativa L.). Investigation in depth is needed to provide an understanding of NPR1 -mediated resistance and a practical strategy for the improvement of disease resistance in the model crop rice. The rice genome contains five NPR1 -like genes. In our study, three rice homologous genes, OsNPR1/NH1, OsNPR2/NH2 and OsNPR3, were found to be induced by rice bacterial blight Xanthomonas oryzae pv. oryzae and rice blast Magnaporthe grisea, and the defence molecules benzothiadiazole, methyl jasmonate and ethylene. We confirmed that OsNPR1 is the rice orthologue by complementing the Arabidopsis npr1 mutant. Over-expression of OsNPR1 conferred disease resistance to bacterial blight, but also enhanced herbivore susceptibility in transgenic plants. The OsNPR1-green fluorescent protein (GFP) fusion protein was localized in the cytoplasm and moved into the nucleus after redox change. Mutations in its conserved cysteine residues led to the constitutive localization of OsNPR1(2CA)-GFP in the nucleus and also abolished herbivore hypersensitivity in transgenic rice. Different subcellular localizations of OsNPR1 antagonistically regulated SA- and jasmonic acid (JA)-responsive genes, but not SA and JA levels, indicating that OsNPR1 might mediate antagonistic cross-talk between the SA- and JA-dependent pathways in rice. This study demonstrates that rice has evolved an SA-mediated systemic acquired resistance similar to that in Arabidopsis, and also provides a practical approach for the improvement of disease resistance without the penalty of decreased herbivore resistance in rice. [source] A bidirectional gene trap construct suitable for T-DNA and Ds -mediated insertional mutagenesis in rice (Oryza sativa L.)PLANT BIOTECHNOLOGY JOURNAL, Issue 5 2004Andrew L. Eamens Summary A construct suitable for genome-wide transfer-DNA (T-DNA) and subsequent transposon-based (Ds) gene trapping has been developed for use in rice (Oryza sativa). This T-DNA/Ds construct contains: Ds terminal sequences immediately inside T-DNA borders for subsequent Ds mobilization; promoterless green fluorescent protein (sgfpS65T) and ,-glucuronidase (uidA) reporter genes, each fused to an intron (from Arabidopsis GPA1 gene) to enable bidirectional gene trapping by T-DNA or Ds; an ampicillin resistance gene (bla) and a bacterial origin of replication (ori) to serve as the plasmid rescue system; an intron-containing hygromycin phosphotransferase gene (hph) as a selectable marker or Ds tracer; and an intron-containing barnase gene in the binary vector backbone (VB) to select against transformants carrying unwanted VB sequences. More than a threefold increase over previously reported reporter gene-based gene trapping efficiencies was observed in primary T-DNA/Ds transformant rice lines, returning an overall reporter gene expression frequency of 23%. Of the plant organs tested, 3.3,7.4% expressed either reporter at varying degrees of organ or tissue specificity. Approximately 70% of the right border (RB) flanking sequence tags (FSTs) retained 1,6 bp of the RB repeat and 30% of the left border (LB) FSTs retained 5,23 bp of the LB repeat. The remaining FSTs carried deletions of 2,84 bp inside the RB or 1,97 bp inside the LB. Transposition of Ds from the original T-DNA was evident in T-DNA/Ds callus lines super-transformed with a transposase gene (Ac) construct, as indicated by gene trap reporter activity and rescue of new FSTs in the resulting double transformant lines. [source] QTL analysis for the resistance to small brown planthopper (Laodelphax striatellus Fallén) in rice using backcross inbred linesPLANT BREEDING, Issue 1 2010C. X. Duan With 3 figures and 3 tables Abstract Small brown planthopper (SBPH) is a serious pest of rice (Oryza sativa L.) in China. An indica variety ,Kasalath' is highly resistant to SBPH. A mapping population consisting of 98 BC1F9 lines, derived from a backcross of ,Nipponbare'/,Kasalath'//,Nipponbare', was applied to detect quantitative trait loci (QTL) for resistance to SBPH. In the modified seedbox screening test, three QTLs for SBPH resistance were mapped on chromosomes 3 and 11, explaining 49.9% of the phenotypic variance. In the antixenosis test, a total of three QTLs conferring antixenosis against SBPH were detected on chromosomes 3, 8 and 11, which accounted for 36.4% of the total phenotypic variance. In addition, two QTLs expressing antibiosis to SBPH were detected on chromosomes 2 and 11, explaining 13.8% and 14.7% of the phenotypic variance, respectively. Qsbph11e, Qsbph11f and Qsbph11g were located in the region between S2260 and G257 on chromosome 11, indicating that the locus is significant in conferring resistance to SBPH in ,Kasalath'. The molecular markers linked to these QTLs should be useful in the development of varieties with horizontal resistance to SBPH. [source] Association mapping of straighthead disorder induced by arsenic in Oryza sativaPLANT BREEDING, Issue 6 2009H. A. Agrama Abstract Straighthead is a physiological disorder in rice (Oryza sativa L.) resulting in sterile florets, poorly developed panicles and yield loss. Because of its sporadic nature and unidentified causes for the disorder, molecular marker assisted selection is essential for resistance improvement in breeding programmes. To take advantage of recent advances in gene-mapping technology, we executed a genome-wide association mapping to identify genetic regions associated with straighthead disorder using 547 accessions of germplasm from the USDA rice core collection. Straighthead was evaluated in arsenic treated soil and genotyping was conducted with 75 molecular markers covering the entire rice genome about every 25 cM. A mixed-linear model approach combining the principal component assignments with kinship estimates proved to be particularly promising for association mapping. The extent of linkage disequilibrium was described among the markers. Six markers were found to be significantly associated with straighthead, explaining 35% of the total phenotypic variation. However, only two SSR markers, RM413 and RM277 on chromosome 5 and 12, respectively, have a significant association with low rating indicating straighthead resistance. Confirmation of the marker-straighthead association using segregating populations is necessary before marker-assisted selection can be applied. [source] | ||||||||||||||||