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Selected AbstractsHuman fetal radial glia cells generate oligodendrocytes in vitroGLIA, Issue 5 2009Zhicheng Mo Abstract Limited knowledge about human oligodendrogenesis prompted us to explore the lineage relationship between cortical radial glia (RG) cells and oligodendrocytes (OLs) in the human fetal forebrain. RG cells were isolated from cortical ventricular/subventricular zone and their progeny was followed in vitro. One portion of RG cells differentiated into cells of OL lineage identified by cell-type specific antibodies, including platelet-derived growth factor receptor-alpha (PDGFR,), NG2, O4, myelin basic protein, and myelin oligodendrocyte glycoprotein. Moreover, using Cre Lox fate mapping (brain lipid binding protein-Cre/Floxed-yellow fluorescent protein) we established a direct link between RG cells and OL progenitors. In vitro generation of RG-derived O4+ OL progenitors was enhanced by addition of sonic hedgehog (SHH) and reduced by the SHH inhibitor, cyclopamine, suggesting the role of SHH signaling in this process. In summary, our in vitro experiments revealed that a portion of cortical RG cells isolated from human forebrain at the second trimester of gestation generates OL progenitors and this suggests a role of SHH in this process. © 2008 Wiley-Liss, Inc. [source] An Alternative Procedure for Fabricating a Hollow Interim Obturator for a Partial Maxillectomy PatientJOURNAL OF PROSTHODONTICS, Issue 3 2009Hiroshi Shimizu DDS Abstract A technique is described for fabricating an interim obturator for a partial maxillectomy patient. This technique enables the immediate and easy chairside fabrication of a lightweight hollow-type interim obturator when a preoperative cast is not available. After a master cast is formed, two duplicate casts are made from the master cast. One portion of the obturator is fabricated on a duplicate cast with a corrected defect, and the other portion of the prosthetic base is made on the second duplicate cast with a corrected alveolar ridge. These two portions are connected and adjusted in the patient's oral cavity. This technique is beneficial because it helps patients maintain good esthetics and their ability to speak, swallow, and chew just after surgery. [source] Effects of Cryopreservation on Bull Spermatozoa Distribution in Morphometrically Distinct SubpopulationsREPRODUCTION IN DOMESTIC ANIMALS, Issue 4 2007J Rubio-Guillén Contents Assisted sperm morphometry analysis (ASMA) was used in this study to determine the effects of cryopreservation on bull spermatozoa distribution in morphometrically distinct subpopulations. Ejaculates were collected from five bulls and were divided. One portion was diluted at 30°C in a skim milk,egg yolk medium, containing glycerol. A microscope slide was prepared from single extended sperm samples prior to freezing. The remainder of each sample was frozen in nitrogen vapours. After thawing, sperm smears were prepared as described above. All slides were air dried and stained with Hemacolor. The sperm-head dimensions for a minimum of 200 sperm heads were analysed from each sample by means of the Sperm-Class Analyser® (SCA), and the mean measurements recorded. Our results showed that applying the ASMA technology and multivariate cluster analyses, it was possible to determine that three separate subpopulations of spermatozoa with different morphometric characteristics coexist in bull ejaculates (large, average and small spermatozoa). The mean values of each sperm head dimension among the three subpopulations of spermatozoa were significantly different (p < 0.001). Besides, there were significant (p < 0.001) differences in the distribution of these three sperm subpopulations between fresh and thawed samples. Thus, the percentage of representation of the subpopulation that includes those spermatozoa whose dimensions are the biggest, decreased from 52.06% in extended fresh samples to 15.51% in the thawed ones. Contrarily, the percent of representation of the subpopulation containing the smallest spermatozoa, increased from 8.70% in extended fresh samples to 34.04% in the thawed ones. In conclusion, the present study confirms the heterogeneity of sperm head dimensions in bull semen, heterogeneity that vary through the cryopreservation procedure. [source] Dynamic scratch-pad memory management with data pipelining for embedded systemsCONCURRENCY AND COMPUTATION: PRACTICE & EXPERIENCE, Issue 13 2010Yanqin Yang Abstract In this paper, we propose an effective data pipelining technique, SPDP (Scratch-Pad Data Pipelining), for dynamic scratch-pad memory (SPM) management with DMA (Direct Memory Access). Our basic idea is to overlap the execution of CPU instructions and DMA operations. In SPDP, based on the iteration access patterns of arrays, we group multiple iterations into a block to improve the data locality of regular array accesses. We allocate the data of multiple iterations into different portions of the SPM. In this way, when the CPU executes instructions and accesses data from one portion of the SPM, DMA operations can be performed to transfer data between the off-chip memory and another portion of SPM simultaneously. We perform code transformation to insert DMA instructions to achieve the data pipelining. We have implemented our SPDP technique with the IMPACT compiler, and conduct experiments using a set of loop kernels from DSPstone, Mibench, and Mediabench on the cycle-accurate VLIW simulator of Trimaran. The experimental results show that our technique achieves performance improvement compared with the previous work. Copyright © 2010 John Wiley & Sons, Ltd. [source] China rose (Hibiscus rosasinensis) petals: a potent natural carotenoid source for goldfish (Carassius auratus L.)AQUACULTURE RESEARCH, Issue 11 2007Archana Sinha Abstract Goldfish (Carassius auratus) are in demand in world markets due to their attractive golden colour. Carotenoids are the primary source of colour in the skin of fish. To optimize the colour in captivity, fish must obtain an adequate level of carotenoids in their feed. With this objective, four natural colour enhancers were tested. A common batch of feed was divided into five equal portions and colour ingredients, spirulina (D-S), china rose petals (D-C), marigold petals (D-M) and Lactobacil, a commercial probiotic (D-L), were added at 5 mg kg,1 to four portions of feed; one portion (D-O) was kept as a control without any additive. A feeding trial was conducted for 8 weeks. Each 70 L aquarium was stocked with 10 fish (average weight 1.6 g) and feed was given at 5% of the body weight. Growth rate, survival, biochemical composition and pigmentation in the skin of fish were measured. Histological studies of gonads were also conducted. Growth of fish in different treatments was significantly different. There was no difference in the proximate composition of the fish at the start of the experiment but after 8 weeks of feeding, fish fed the diet supplemented with china rose petals had a lower moisture content (70.48%) and higher protein (17.7%) and lipid (5.25%) levels than the group fed the control diet. Pigmentation was the highest (4.01 ,g g,1) in D-C, followed by D-M (3.16 ,g g,1), D-S (2.92 ,g g,1) and D-L (2.84 ,g g,1) and the lowest (0.24 ,g g,1) in D-O. Gonad development of fish fed with the D-C diet was better compared with the gonads of control (D-O) fish, followed by D-M-, D-L- and D-S-fed fish. Gonads of fish, fed D-C, showed well-marked changes in the testis where a large number of seminiferous tubules bound together by means of a thin layer of connective tissue were observed. These tubules were highly convoluted and were separated from each other by thin connective tissue stroma. The intra space contained connective tissue, blood capillaries and interstitial cells. The spermatogonia could be seen as a large spherical cell containing a large central nucleus with a distinct nucleolus. The study shows that the china rose (Hibiscus rosasinensis) petal is a potent natural carotenoid source for goldfish to enhance its colour and also accelerate gonadal development. [source] | ||||||||||