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Ocular Cicatricial Pemphigoid (ocular + cicatricial_pemphigoid)
Selected AbstractsTissue-engineered tear secretory system: Functional lacrimal gland acinar cells cultured on matrix protein-coated substrataJOURNAL OF BIOMEDICAL MATERIALS RESEARCH, Issue 1 2007Shivaram Selvam Abstract Dry eye is a general term that refers to a myriad of ophthalmic disorders resulting in the inadequate wetting of the corneal surface by the tear film. Dry eyes are typically treated by the application of artificial tears. However, patients with lacrimal insufficiencies such as Stevens-Johnson syndrome, chemical and thermal injuries, or ocular cicatricial pemphigoid have very limited options because of the short duration and action of lubricating agents. As a therapeutic strategy, we are working to develop a bioengineered tear secretory system for such patients. This article describes the growth and physiological properties of purified rabbit lacrimal gland acinar cells (pLGACs) on several matrix protein-coated polymers such as silicone, collagen I, copolymers of poly- D,L -lactide- co -glycolide (PLGA; 85:15 and 50:50), poly- L -lactic acid (PLLA), and Thermanox® plastic cell culture coverslips. Monolayers of acinar cells were established on all of the polymeric substrata. An assay of ,-hexosaminidase activity in the supernatant medium showed significant increases in protein secretion, following stimulation with 100 ,M carbachol on matrix protein-coated and uncoated polymers such as silicone, PLGA 85:15, and PLLA. Our study demonstrates that PLLA supported the morphological and physiological properties of purified rabbit lacrimal gland epithelial cells more successfully than the others. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007 [source] Tumour necrosis factor-, in conjunctivae affected by ocular cicatricial pemphigoidACTA OPHTHALMOLOGICA, Issue 7 2007Miguel Cordero Coma Abstract. Purpose:, The presence of tumour necrosis factor-, (TNF-,) in conjunctivae affected by ocular cicatricial pemphigoid (OCP) was investigated. Methods:, Biopsy specimens from the conjunctivae of eight patients with OCP, three patients with atopic keratoconjunctivitis (AKC) and two normal subjects were studied for the expression of TNF-, by immunohistochemistry. Two independent, masked investigators evaluated the specimens. All samples were similarly processed by a third investigator. Results:, No TNF-, was discerned in the normal conjunctival sections; small amounts of TNF-, were observed in the atopic keratoconjunctivitis specimens. TNF-, was present in substantial amounts in conjunctival sections of patients with OCP. The expression of TNF-, was detected in both epithelial and stromal cells of conjunctivae from OCP patients. Conclusions:, The presence of TNF-, in conjunctivae affected by OCP may indicate that this cytokine plays an important role in the production and maintenance of conjuctival inflammation response and subsequent conjunctival scarring in patients with OCP. Further studies clarifying this potential role are warranted. [source] Cicatricial entropion repair with hard palate mucous membrane graft: surgical technique and outcomesCLINICAL & EXPERIMENTAL OPHTHALMOLOGY, Issue 4 2008Brighu N Swamy Abstract Background:, The use of a hard palate mucous membrane graft (HPMMG) has been previously described for upper and lower eyelid cicatricial entropion repair. The objective of this paper is to review the surgical technique and postoperative complications in a large series of patient who underwent hard palate grafting for the management of cicatricial entropion. Methods:, The medical records of 107 patients representing 147 eyelids undergoing surgical management of cicatricial entropion with HPMMG were reviewed. The surgical technique is described. Results:, 147 eyelid operations (74 upper, 73 lower) were performed on 107 patients (46 male, 61 female), with a mean age of 63 years (range 12,87). The aetiology of the cicatricial entropion included idiopathic (41%), trauma (5.6%), chronic blepharitis (16.8%), chemical injury (3.7%), ocular cicatricial pemphigoid (8.4%), trachoma (7.5%) and other (16.8%). Patients were followed postoperatively for an average of 21 months (range 6,120). Ninety-four per cent of patients noted symptomatic improvement. The postoperative complications included excess keratin (29%), recurrence of cicatricial entropion (4.1%), punctuate epithelial erosion (2.7%), graft shrinkage (0.7%) and donor site bleeding (2.0%). Conclusions:, Cicatricial Entropion with hard palate mucous membrane grafting for both upper and lower eyelid surgery offers high symptomatic and anatomical cure rates. The requirement for further surgical intervention is low. [source] | ||||||||||