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Oil Seeds (oil + seed)
Selected AbstractsActivity and concentration of non-proteolyzed phosphoenolpyruvate carboxykinase in the endosperm of germinating castor oil seeds: effects of anoxia on its activityPHYSIOLOGIA PLANTARUM, Issue 4 2007Mariana Martín Phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.49) catalyses the reversible decarboxylation of oxaloacetate to phosphoenolpyruvate in the gluconeogenic production of sugars from storage lipids in germinating oil seeds. The enzyme is quite susceptible to limited proteolysis during extraction. Immunoblotting was used to diagnose unwanted in vitro proteolytic activity against PEPCK from germinating castor oil seeds (COS) by following the disappearance of its native 74-kDa subunit and concomitant appearance of a truncated 64-kDa polypeptide. Alkaline pH and the inclusion of thiol protease inhibitors effectively prevented COS PEPCK proteolysis during incubation of clarified COS extracts at 4°C. The carboxylating and decarboxylating activities and concentration of non-proteolyzed COS PEPCK were investigated during germination. This is the first report in which both activities catalyzed by PEPCK were measured in vitro during a whole developmental process. Carboxylating activity and the level of immunoreactive 74-kDa PEPCK polypeptides rapidly increased in parallel to maximal values by day 5 and then significantly declined over the subsequent 2 days. In contrast, decarboxylating PEPCK activity was much higher over the 7 days of growth examined. In addition, the effect on PEPCK activity while changing the endosperm from aerobic (when gluconeogenesis predominates in the tissue) to anaerobic conditions (where the tissue becomes glycolytic) was studied. While PEPCK decarboxylating activity remained almost constant, carboxylating activity declined to undetectable levels in response to anaerobiosis. These and the developmental profile results suggest that COS PEPCK may be subject to a mechanism of post-translation control that selectively inhibits the carboxylating, but not the decarboxylating activity. [source] Chemical characterization of Azadirachta indica grafted on Melia azedarach and analyses of azadirachtin by HPLC-MS-MS (SRM) and meliatoxins by MALDI-MSPHYTOCHEMICAL ANALYSIS, Issue 4 2010Moacir Rossi Forim Abstract Introduction , Melia azedarach adapted to cool climates was selected as rootstocks for vegetative propagation of Azadirachta indica. Cleft grafting of A. indica on M. azedarach rootstock showed excellent survival. Little is known about the chemistry of grafting. Objective , The roots, stems, leaves and seeds of this graft were examined in order to verify if grafted A. indica would produce limonoids different from those found in non-grafted plants. Intact matured fruits were also studied to verify if they were free of meliatoxins. Methodology , After successive chromatographic separations the extracts afforded several limonoids. HPLC-MS/MS and MALDI-MS were used to develop sensitive methods for detecting azadirachtin on all aerial parts of this graft and meliatoxins in fruits, respectively. Results , The stem afforded the limonoid salannin, which was previously found in the oil seeds of A. indica. Salannin is also found in the root bark of M. azedarach. Thus, the finding of salannin in this study suggests that it could have been translocated from the M. azedarach rootstock to the A. indica graft. HPLC-MS/MS analyses showed that azadirachtin was present in all parts of the fruits, stem, flowers and root, but absent in the leaves. The results of MALDI-MS analyses confirmed the absence of meliatoxins in graft fruits. Conclusion , This study showed that A. indica grafted onto M. azedarach rootstock produces azadirachtin, and also that its fruits are free of meliatoxins from rootstocks, confirming that this graft forms an excellent basis for breeding vigorous Neem trees in cooler regions. Copyright © 2010 John Wiley & Sons, Ltd. [source] Potential of Pakistani camel for dairy and other usesANIMAL SCIENCE JOURNAL, Issue 5 2007Muhammad YAQOOB ABSTRACT Camels have the potential for milk, meat and draught power and can contribute a handsome share of the production of these commodities. The potential of this wonderful animal has never been realized and it could be harnessed as a prospective milk producing animal. The future of animals that can thrive under harsh environmental conditions, the camel being at the top of the list, is bright. The camel is still a neglected species in Pakistan and has not received the proper attention of researchers and scientists. The population explosion, urbanization and industrialization have expanded agricultural activities to produce more food for the rapid growing human population of the country. Cultivated areas are shrinking, thus reducing the fodder production area for buffalo and cattle. Under these circumstances we have to search for other available sources to enhance milk production. The environmental changes occurring on the earth and the water shortage in the region have also adversely affected the production potential of buffalo, cattle, goats and sheep. Under these changing ecological circumstances, rearing camel is the best option for more milk production and the proper utilization of the vast unused lands of this country. Most studies also have named the camel as an animal of great socioeconomic importance in large tracts of the industrializing world. The camel serves as a cheaper source of power for drawing water from wells, plowing and leveling land, working mini extraction mills (extracting from oil seeds), grinding wheat, corn and other grains and crushing sugarcane and pulling carts for the transportation of goods as well as people. [source] Extraction of Poppy Seed Oil Using Supercritical CO2JOURNAL OF FOOD SCIENCE, Issue 2 2003B. Bozan ABSTRACT: Extraction of poppy seed oil with supercritical carbon dioxide (SC-CO2) was performed and the effect of extraction conditions on oil solubility and yield as well as oil composition was evaluated. Within the temperature (50 to 70 °C) and pressure (21 to 55 MPa) ranges studied, 55 MPa/70 °C gave the highest oil solubility (24.1 mg oil/g CO2) and oil yield (38.7 g oil/100g seed). Fatty acid composition of the oil obtained with SC-CO2 at 55 MPa/70 °C was similar to that of petroleum ether-extracted oil (p > 0.05) with linoleic acid making up 69.0 to 73.7% of fatty acids. Tocol content of the SC-CO2 -extracted oils varied from 22.37 to 33.35 mg/100 g oil, which was higher than that of petroleum ether-extracted oil (15.28 mg/100 g oil). Poppy seed oil may have potential in the rapidly growing specialty oil market. [source] | |||||||||||||