Home About us Contact
|
Home About us Contact | ||
|
|
||
Novel Polymorphic Microsatellite Markers (novel + polymorphic_microsatellite_marker)
Selected AbstractsNovel polymorphic microsatellite markers developed in the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae)INSECT SCIENCE, Issue 5 2005YA-JIE JI Abstract A novel set of five polymorphic di- or trinucleotide microsatellite loci suitable for population genetic study were developed from an enriched genomic library for the pest insect cotton bollworm, Helicoverpa armigera, and cross-amplifiability of these and other published loci was tested in a closely related species, the tobacco budworm, H. assulta. The expected heterozygosity at these loci ranges from 0.62 to 0.91 in the cotton bollworm. The observed allele numbers varies from 4 to 12 in the limited number of individuals tested. Although a large proportion of cloned microsatellite sequences are present in multi-copy in the cotton bollworm genome, the overwhelming majority of the finalized polymorphic diallelic loci are tri-nucleotide microsatellites - an unexpected outcome, which should facilitate subsequent genotyping analysis. [source] Novel polymorphic microsatellite markers for paternity analysis in the red-capped robin (Petroica goodenovii: Aves)MOLECULAR ECOLOGY RESOURCES, Issue 4 2003D. K. Dowling Abstract Seven microsatellite loci were isolated and characterized from the red-capped robin Petroica goodenovii, using nonradioactive polymerase chain reaction (PCR)-based techniques to screen an enriched genomic library. Five loci showed no evidence of null alleles and were variable [mean heterozygosity (HE) = 0.440, mean number of alleles = 8]. Cross-amplification using primers for microsatellites in Phylloscopus occipitalis and Emberiza schoeniclus yielded another two polymorphic loci. The combined set of five red-capped robin and two cross-amplified loci are suitable for paternity assignment (exclusion probability for seven unlinked loci = 0.9760). [source] Novel polymorphic microsatellite markers for the common pandora (Pagellus erythrinus)MOLECULAR ECOLOGY RESOURCES, Issue 4 2003Andreja Ram Abstract Details of six highly polymorphic dinucleotide and one highly polymorphic tetranucleotide microsatellite markers are provided for Pagellus erythrinus. These markers are highly polymorphic, with an expected heterozygosity ranging from 0.713 to 0.959 and allele numbers ranging from seven to 36. These microsatellite markers should help determine population genetic structure and fisheries stocks for management purposes. [source] Isolation and characterization of microsatellite markers from Teretrius nigrescens Lewis (Coleoptera: Histeridae), predator of the storage pest Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae)MOLECULAR ECOLOGY RESOURCES, Issue 4 2009A. B. OMONDI Abstract Teretrius nigrescens is a predator of the larger grain borer (LGB) Prostephanus truncatus, an invasive post-harvest pest in Africa. We describe the isolation and characterization of 24 novel polymorphic microsatellite markers and their testing on a population from Honduras. Alleles per locus ranged between 2 and 12, and observed heterozygosity between 0.037 and 0.646. Six loci deviated significantly from Hardy,Weinberg equilibrium and showed evidence of null alleles. These markers will be useful for studies of the predator's population structure and characterizing populations for control of LGB. [source] | ||||||||||