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Novel Platform (novel + platform)
Selected AbstractsFunctionalized 3,3,,5,5,-Tetraaryl-1,1,-Biphenyls: Novel Platforms for Molecular ReceptorsHELVETICA CHIMICA ACTA, Issue 3 2003Roger Welti This paper describes the development of novel aromatic platforms for supramolecular construction. By the Suzuki cross-coupling protocol, a variety of functionalized m- terphenyl derivatives were prepared (Schemes,1,4). Macrolactamization of bis(ammonium salt) (S,S)- 6 with bis(acyl halide) 7 afforded the macrocyclic receptor (S,S)- 2 (Scheme,1), which was shown by 1H-NMR titration studies to form ,nesting' complexes of moderate stability (Ka between 130 and 290,M,1, 300,K) with octyl glucosides 13,15 (Fig.,2) in the noncompetitive solvent CDCl3. Suzuki cross-coupling starting from 3,3,,5,5,-tetrabromo-1,1,-biphenyl provided access to a novel series of extended aromatic platforms (Scheme,5) for cleft-type (Fig.,1) and macrotricyclic receptors such as (S,S,S,S)- 1. Although mass-spectral evidence for the formation of (S,S,S,S)- 1 by macrolactamization between the two functionalized 3,3,,5,5,-tetraaryl-1,1,-biphenyl derivatives (S,S)- 33 and 36 was obtained, the 1H- and 13C-NMR spectra of purified material remained rather inconclusive with respect to both purity and constitution. The versatile access to the novel, differentially functionalized 3,3,,5,5,-tetrabromo-1,1,-biphenyl platforms should ensure their wide use in future supramolecular construction. [source] Comprehensive expression atlas of fibroblast growth factors and their receptors generated by a novel robotic in situ hybridization platformDEVELOPMENTAL DYNAMICS, Issue 2 2005Murat Burak Yaylaoglu Abstract A recently developed robotic platform termed "Genepaint" can carry out large-scale nonradioactive in situ hybridization (ISH) on tissue sections. We report a series of experiments that validate this novel platform. Signal-to-noise ratio and mRNA detection limits were comparable to traditional ISH procedures, and hybridization was transcript-specific, even in cases in which probes could have hybridized to several transcripts of a multigene family. We established an atlas of expression patterns of fibroblast growth factors (Fgfs) and their receptors (Fgfrs) for the embryonic day 14.5 mouse embryo. This atlas provides a comprehensive overview of previously known as well as novel sites of expression for this important family of signaling molecules. The Fgf/Fgfr atlas was integrated into the transcriptome database (www.genepaint.org), where individual Fgf and Fgfr expression patterns can be interactively viewed at cellular resolution and where sites of expressions can be retrieved using an anatomy-based search. Developmental Dynamics 234:371,386, 2005. © 2005 Wiley-Liss, Inc. [source] Capturing Complex Protein Gradients on Biomimetic Hydrogels for Cell-Based AssaysADVANCED FUNCTIONAL MATERIALS, Issue 21 2009Steffen Cosson Abstract A versatile strategy to rapidly immobilize complex gradients of virtually any desired protein on soft poly(ethylene glycol) (PEG) hydrogel surfaces that are reminiscent of natural extracellular matrices (ECM) is reported. A microfluidic chip is used to generate steady-state gradients of biotinylated or Fc-tagged fusion proteins that are captured and bound to the surface in less than 5,min by NeutrAvidin or ProteinA, displayed on the surface. The selectivity and orthogonality of the binding schemes enables the formation of parallel and orthogonal overlapping gradients of multiple proteins, which is not possible on conventional cell culture substrates. After patterning, the hydrogels are released from the microfluidic chip and used for cell culture. This novel platform is validated by conducting single-cell migration experiments using time-lapse microscopy. The orientation of cell migration, as well as the migration rate of primary human fibroblasts, depends on the concentration of an immobilized fibronectin fragment. This technique can be readily applied to other proteins to address a wealth of biological questions with different cell types. [source] Engineered bio-nanocapsules, the selective vector for drug delivery systemIUBMB LIFE, Issue 1 2006Dongwei Yu Abstract The bio-nanocapsule (BNC) is our concept of artificial hollow nanoparticles that have been designed and produced through biotechnological procedures. We proposed an empty virus-like particle, which consists of a recombinant L envelope protein of hepatitis B virus (HBV) and a lipid derived from the host cell, as an engineered BNC. Although this BNC was first developed as an immunogen of hepatitis B vaccine, the pre-S1 region in N-terminus of L envelope protein confers hepatocyte specific infectivity of HBV on the BNC. This recombinant BNC is now being developed as a novel platform of drug delivery system (DDS) vector for selective delivery. IUBMB Life, 58: 1 - 6, 2006 [source] Triclosan inhibition of mycobacterial InhA in Saccharomyces cerevisiae: yeast mitochondria as a novel platform for in vivo antimycolate assaysLETTERS IN APPLIED MICROBIOLOGY, Issue 4 2010A. Gurvitz Abstract Aims:, To demonstrate the suitability of yeast to act as a novel biotechnological platform for conducting in vivo inhibition assays using drugs with low efficacies towards their mycobacterial targets, such as occurs in the situation with triclosan and InhA. Methods and Results:, A surrogate yeast host represented by Saccharomyces cerevisiae etr1, cells lacking Etr1p, the 2- trans -enoyl-thioester reductase of mitochondrial type 2 fatty acid synthase (FASII), was designed to rely on the Mycobacterium tuberculosis FASII enzyme InhA. Although InhA is 10 000 times less sensitive to the antimicrobial drug triclosan than is bacterial FabI, the respiratory growth of yeast cells depending on InhA was severely affected on glycerol medium containing triclosan. Conclusions:, The yeast system could detect enzyme inhibition despite the use of a drug with only low efficacy. Significance and Impact of the Study:, Tuberculosis affects a third of the human population, and InhA is a major drug target for combating this disease. InhA is inhibited by isoniazid, but triclosan-derived compounds are presently being developed as antimycolates. A demonstration of triclosan inhibition of InhA in yeast represents a meaningful variation in studying this effect in mycobacteria, because it occurred without the potentially confusing aspects of perturbing protein,protein interactions which are presumed vital to mycobacterial FASII, inactivating other important enzymes or eliciting a dedicated transcriptional response in Myco. tuberculosis. [source] A Self-assembly Route for Double Bilayer Lipid Membrane FormationCHEMPHYSCHEM, Issue 3 2010Xiaojun Han Dr. Biomembranes: A new method of forming double bilayer lipid membranes uses NHS/EDC chemistry to link adjacent bilayers, both of which remain fluid. This approach provides a novel platform for the study of biomembranes, in which the components of the upper membrane are shielded from the solid substrate by a second membrane (see figure); and for studying more complex membrane protein systems which span double lipid bilayers. [source] | |||||||||||||