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New Microsatellite Markers (new + microsatellite_marker)
Selected AbstractsGiant Axonal Neuropathy Locus Refinement To A < 590 KB Critical IntervalJOURNAL OF THE PERIPHERAL NERVOUS SYSTEM, Issue 1 2001L Cavalier Giant axonal neuropathy (GAN) is a rare autosomal recessive neurodegenerative disorder, characterised clinically by the development of chronic distal polyneuropathy during childhood, mental retardation, kinky or curly hair, skeletal abnormalities and, ultrastructurally, by axons in the central and peripheral nervous systems distended by masses of tightly woven neurofilaments. We recently localised the CAN locus in 16q24.1 to a 5-cM interval between the D16S507 and D16S511 markers by homozygosity mapping in three consanguineous Tunisian families. We have now established a contig-based physical map of the region comprising YACs and BACs where we have placed four genes, ten ESTs, three STSs and two additional microsatellite markers, and where we have identified six new SSCP polymorphisms and six new microsatellite markers. Using these markers, we have refined the position of our previous flanking recombinants. We also identified a shared haplotype between two Tunisian families and a small region of homozygosity in a Turkish family with distant consanguinity, both suggesting the occurrence of historic recombinations and supporting the conclusions based on the phase-known recombinations. Taken together, these results allow us to establish a transcription map of the region, and to narrow down the GAN position to a < 590 kb critical interval, an important step toward the identification of the defective gene. [source] Isolation and characterization of new microsatellite markers for rose bitterlings, Rhodeus ocellatusMOLECULAR ECOLOGY RESOURCES, Issue 3 2009Y. SHIRAI Abstract The Japanese rose bitterling (Rhodeus ocellatus kurumeus) is facing imminent extinction because of hybridization and competition from an invasive alien subspecies (Rhodeus ocellatus ocellatus). Eleven new microsatellite markers for the two subspecies were developed using dinucleotide repeat specific polymerase chain reaction. The number of alleles per locus and the heterozygosity in R. o. kurumeus were lower than those in R. o. ocellatus. Most of these microsatellite markers were successfully cross-amplified in three Acheilognathinae species. [source] Isolation and characterization of microsatellite loci for the European tree frog (Hyla arborea)MOLECULAR ECOLOGY RESOURCES, Issue 5 2008L. BERSET-BRäNDLI Abstract We developed 11 new microsatellite markers for the European tree frog (Hyla arborea), and tested patterns of polymorphism in 54 adults (27 males and 27 females) from two ponds close to Lausanne (Western Switzerland). One marker was sex linked and two pairs displayed linkage disequilibrium. Comparisons of allele numbers with heterozygosity values support a stepwise-mutation model at neutral equilibrium, with mutation rates spanning nearly two orders of magnitude. These markers will prove useful for population genetic studies and fine-scale investigations requiring genetic assignment techniques. [source] Isolation and characterization of new microsatellite markers in shea tree (Vitellaria paradoxa C. F. Gaertn)MOLECULAR ECOLOGY RESOURCES, Issue 4 2008FRANÇOIS ALLAL Abstract Vitellaria paradoxa is one of the major components of African parkland agroforestry systems. In order to assess the genetic diversity and population structure of this species, we isolated and characterized 14 polymorphic nuclear microsatellite loci. Primers developed to amplify these loci were used to analyse 200 individuals of a shea tree population in Mali. Loci have shown a high number of alleles ranging from four to 26, and display an observed level of heterozygosity between 0.37 and 0.85. These new very polymorphic microsatellite markers will be useful for genetic and ecological studies of V. paradoxa. [source] PRIMER NOTE: Microsatellite markers for the June sucker (Chasmistes liorus mictus), Utah sucker (Catostomus ardens), and five other catostomid fishes of western North AmericaMOLECULAR ECOLOGY RESOURCES, Issue 3 2007B. L. CARDALL Abstract We developed and optimized five new microsatellite markers for the genetic management of the endangered June sucker. We report the cross-amplification of these markers, and seven microsatellites previously developed for Klamath Basin suckers, in seven catostomid species of western North America. No linkage disequilibrium was detected between pairs of loci. Since most of these loci exhibited conserved priming sites, they may be useful for landscape-scale studies of speciation and patterns of gene flow among multiple sucker lineages. [source] Development and characterization of eight new microsatellite markers for the haremic sandperch, Parapercis cylindrica (family Pinguipedidae)MOLECULAR ECOLOGY RESOURCES, Issue 4 2006C. SMITH-KEUNE Abstract Eight di-, tri- and tetranucleotide microsatellite markers were developed for the haremic sandperch Parapercis cylindrica using a linker-ligated, magnetic bead enrichment protocol. Screening of at least 17 individuals showed these markers to be polymorphic with observed heterozygosity ranging from 0.381 to 1.000 (mean = 0.742) and the numbers of alleles ranging from three to 18. The average polymorphic information content for these eight loci was 0.723. These markers may be used for parentage studies aimed at exploring the complex mating strategies employed by this haremic coral reef fish and will be valuable for population genetic studies. [source] Characterization of new microsatellite markers in mung bean, Vigna radiata (L.)MOLECULAR ECOLOGY RESOURCES, Issue 4 2006JAE-GYUN GWAG Abstract The present work reports the isolation and characterization of new polymorphic microsatellites in mung bean (Vigna radiata L.). Of 93 designed primer pairs, seven were found to amplify polymorphic microsatellite loci, which were then characterized using 34 mung bean accessions. The number of alleles ranged from two to five alleles per locus with an average of three alleles. Observed and expected heterozygosity values ranged from 0 to 0.088 and from 0.275 to 0.683, respectively. All seven loci showed significant deviations from Hardy,Weinberg equilibrium, whereas only one pairwise combination (GBssr-MB77 and GBssr-MB91) exhibited significant departure from linkage disequilibrium. These newly developed markers are currently being utilized for diversity assessment within the mung bean germplasm collection of the Korean Gene Bank. [source] Development and characterization of nine new microsatellite markers in Taraxacum (Asteraceae)MOLECULAR ECOLOGY RESOURCES, Issue 4 2004RADIM J. VA Abstract This study aims at developing and characterizing new microsatellite primer pairs in Taraxacum officinale auct. to produce polymorphic markers for genetical and evolutionary studies on apomixis in this sexual-apomictic complex. A total of 24 diploid plants were tested for allelic polymorphism and heterozygosity. Out of nine loci three deviated significantly from the Hardy,Weinberg equilibrium expectations, probably due to the presence of null-alleles. Successful cross-species amplification was obtained for all markers in 29 Taraxacum microspecies from five sections. [source] Five new microsatellite markers for Northern pike (Esox lucius)MOLECULAR ECOLOGY RESOURCES, Issue 3 2003Sophie Launey Abstract Five new microsatellite markers were isolated from Northern pike (Esox lucius), a freshwater fish widely distributed in both Northern Europe and Northern America. Characterization in 30 individuals from a population from France revealed four to seven alleles and an expected heterozygosity of 0.243,0.642. No departure from Hardy,Weinberg equilibrium nor linkage disequilibrium were found. The level of polymorphism was higher than previously reported in the literature for pike populations from Northern America and Northern Europe, but with different microsatellite loci. [source] Isolation of new microsatellite markers and application in four species of mouse lemurs (Microcebus sp.)MOLECULAR ECOLOGY RESOURCES, Issue 2 2003A. Hapke Abstract We report the development of 13 new microsatellite markers for mouse lemurs (Microcebus sp.). Two markers were isolated from the fat tailed dwarf lemur (Cheirogaleus medius) and 11 from the grey mouse lemur (Microcebus murinus). A total of 561 individuals from four different species of mouse lemurs was genotyped with the newly developed markers. All markers showed Mendelian inheritance in 21 families of mouse lemurs. All markers show polymorphism in several species of mouse lemurs and seven amplified in C. medius. Among these new markers are the first 10 published for M. berthae and the first 11 for M. griseorufus. [source] Quantitative resistance to Plum pox virus in Prunus davidiana P1908 linked to components of the eukaryotic translation initiation complexPLANT PATHOLOGY, Issue 3 2009G. Marandel A complex, polygenic resistance to Plum pox virus (PPV) was previously described in a wild peach-related species, Prunus davidiana clone P1908. In the current study, an analysis of quantitative trait loci (QTL) was performed on an F2 population comprising 99 individuals obtained by selfing the F1 individual #40 of an interspecific cross between susceptible nectarine cv. Summergrand and the resistant P. davidiana clone P1908. Six QTL were identified using both parametric and non-parametric methods of detection, individually explaining 5,28% of the phenotypic variance. The total phenotypic variation explained ranged from 29 to 58%. Alignment of the genetic map of the F2 cross with the P. davidiana parent map showed consistency of QTL over generations, with three of the six QTL co-localizing at the 1-LOD interval and another one at the 2-LOD interval. Two of the QTL were mapped onto linkage group one, where resistance to PPV was previously mapped in apricot. Development and mapping of new microsatellite markers linked to candidate genes revealed a striking co-localization of three of the detected QTL with gene copies coding for eukaryotic translation initiation factors eIF4E and eIF(iso)4G. As co-localization of one QTL with candidate gene eIF(iso)4E was previously reported in the F1 population, the results reported here strongly reinforce the idea that components of the eukaryotic translation initiation complex are correlated with resistance to PPV in P. davidiana P1908. [source] Polymorphic microsatellite loci for the common marmoset (Callithrix jacchus) designed using a cost- and time-efficient methodAMERICAN JOURNAL OF PRIMATOLOGY, Issue 9 2008M. Raveendran Abstract We describe a cost- and time-efficient method for designing new microsatellite markers in any species with substantial genomic DNA sequence data available. Using this technique, we report 14 new polymorphic dinucleotide microsatellite loci isolated from the common marmoset. The relative yield of new polymorphisms was higher with less labor than described in previous marmoset studies. Of 20 loci initially evaluated, 14 were polymorphic and amplified reliably (70% success rate). The number of alleles ranged from 3 to 9 with heterozygosity varying from 0.48 to 0.83. Am. J. Primatol. 70:906,910, 2008. © 2008 Wiley-Liss, Inc. [source] Development of 21 new microsatellite markers in Labeo rohita (rohu)ANIMAL GENETICS, Issue 2 2009A. Patel No abstract is available for this article. [source] A Generalized Caprine-like Hypoplasia Syndrome is localized within a 6-cM interval on bovine chromosome 13 in the Montbéliarde breedANIMAL GENETICS, Issue 2 2008A. Duchesne Summary Caprine-like Generalized Hypoplasia Syndrome (or SHGC) is a new hereditary disorder described in the Montbéliarde breed. We report here the characterization of this new disease, based on the visual examination of animals affected by SHGC, and on physiological and biochemical studies undertaken on samples of both SHGC and normal animals. Biological samples for more than 150 affected calves and their parents have been collected over the past 4 years within the framework of the Bovine Genetic Disease Observatory. First, pedigree analyses showed that the mode of inheritance is most probably autosomal recessive. Then, a genome scan with 113 animals and 140 microsatellite markers revealed a single locus within a 35-cM region on bovine chromosome 13. Genotypes of 261 animals for 18 new microsatellite markers from the region confirmed the localization of the disorder to a 6-cM interval. Finally, based on the analysis of haplotypes in 463 Montbéliarde sires, we estimated the frequency of the SHGC mutated allele in the population and could propose a strategy for the systematic eradication of this disorder in the near future. [source] Development and linkage relationships for new microsatellite markers of the sea bass (Dicentrarchus labrax L.)ANIMAL GENETICS, Issue 1 2004D. A. Chistiakov Summary Twenty-eight polymorphic microsatellites were isolated from the sea bass, Dicentrarchus labrax, using a microsatellite enrichment protocol and selective hybridization with oligonucleotide probes. Analysis for these markers and 11 recently described microsatellites of D. labrax found linkage between 26 loci and revealed eight linkage groups. [source] | ||||||||||