Home About us Contact
|
Home About us Contact | ||
|
|
||
New Member (new + member)
Terms modified by New Member Selected AbstractsTwo C-Terminal Variants of NBC4, a New Member of the Sodium Bicarbonate Cotransporter Family: Cloning, Characterization, and LocalizationIUBMB LIFE, Issue 1 2000Alexander Pushkin Abstract We report the cloning, characterization, and chromosomal assignment of a new member of the sodium bicarbonate cotransporter (NBC) family, NBC4. The NBC4 gene was mapped to chromosome 2p13 and is a new candidate gene for Alstrom syndrome. Two variants of the transporter have been isolated from human testis and heart, which differ in their C termini. NBC4a encodes a 1137-residue polypeptide and is widely expressed in various tissues, including liver, testis, and spleen. NBC4b is identical to NBC4a except that it has a 16-nucleotide insert, creating a C-terminal frame shift. NBC4b encodes a 1074-residue polypeptide and is highly expressed in heart. Amino acids 1-1046 are common to both NBC4 variants. NBC4a has two protein-interacting domains that are lacking in NBC4b: a proline-rich sequence, PPPSVIKIP (amino acids 1102-1110), and a consensus PDZ-interacting domain, SYSL (1134-1137). NBC4b lacks the stretch of charged residues present in the C terminus of NBC4a and other members of the NBC family.Unlike other members of the NBC family, both NBC4a and NBC4b have a unique glycine-rich region (amino acids 440- 469). In comparison with other members of the bicarbonate transport superfamily, NBC4a and NBC4b are most similar structurally to the electrogenic sodium bicarbonate cotransporters (NBC1). [source] ECONOMIC GROUPINGS: EAC: New Member?AFRICA RESEARCH BULLETIN: ECONOMIC, FINANCIAL AND TECHNICAL SERIES, Issue 7 2010Article first published online: 1 SEP 2010 No abstract is available for this article. [source] F90927: A New Member in the Class of Cardioactive SteroidsCARDIOVASCULAR THERAPEUTICS, Issue 3 2007Markus Keller ABSTRACT F90927 is a newly developed cardioactive drug with a steroid-like structure. It acts directly and agonistically on the cardiac L-type Ca2+ channel by shifting its voltage-dependent activation toward more negative potentials. This leads to an increased influx of Ca2+ and, therefore, to a stronger contraction; however, no arrhythmias occur. Calcium current stimulation can already be observed at nanomolar concentrations, but higher concentrations of F90927 elevate intracellular Ca2+ concentration, causing a reduction of the myocardial compliance and an increased diastolic blood pressure. Vessels also react to F90927 and contract in its presence. Binding of F90927 with the L-type Ca2+ channel presumably occurs in the vicinity of the transmembrane domains III and IV of the ,1 subunit. F90927 exhibits no use dependence and interacts with Ca2+ channel inhibitors of all three known classes of channel modulators (dihydropyridines, phenylalkylamines, and benzothiazepines), suggesting that it is a member of a new class of Ca2+ channel modulators. Due to its adverse effects on blood pressure and vessel contraction, F90927 is not an ideal drug candidate. It has, however, some unique properties, which makes it a promising tool to study the function of the L-type Ca2+ channel. [source] ChemInform Abstract: Synthesis of a New Member in Iron-Based Layered Superconductor: Nd0.8Th0.2OFeAs with Tc = 38 K.CHEMINFORM, Issue 9 2009Min Xu Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a "Full Text" option. The original article is trackable via the "References" option. [source] Crystallographic Features and Tetragonal Phase Stability of PbVO3, a New Member of PbTiO3 Family.CHEMINFORM, Issue 17 2005Alexei A. Belik Abstract For Abstract see ChemInform Abstract in Full Text. [source] Crystal Structure and Magnetic Properties of the Trilayered Perovskite Sr4Rh3O10: A New Member of the Strontium Rhodate Family.CHEMINFORM, Issue 44 2004Kazunari Yamaura Abstract For Abstract see ChemInform Abstract in Full Text. [source] Editorial: Electroanalyses , Welcome to New Members of the Editorial TeamELECTROANALYSIS, Issue 1 2010Joseph Wang No abstract is available for this article. [source] Announcing Two New Members of the Foreign Language Annals Editorial BoardFOREIGN LANGUAGE ANNALS, Issue 1 2007Article first published online: 31 DEC 200 No abstract is available for this article. [source] New Members in the Nin+1(QO3)nX2 Family: Unusual 3D Network Based on Ni4ClO3 Cubane-Like Clusters in Ni7(TeO3)6Cl2.CHEMINFORM, Issue 50 2006Hai-Long Jiang Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF. [source] High Pressure Synthesis and Characterization of New Members of the RuSr2(Ln, Ce)2Cu2O10 Family (Ln: Y, Dy).CHEMINFORM, Issue 44 2005Jose Manuel Gallardo-Amores Abstract For Abstract see ChemInform Abstract in Full Text. [source] ChemInform Abstract: First Rb Silicotitanate Phase and Its K-Structural Analogue: New Members of the SNL-A Family (Cc-A2TiSi6O15; A: K, Rb, Cs).CHEMINFORM, Issue 8 2002M. Nyman Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a "Full Text" option. The original article is trackable via the "References" option. [source] Rho-GTPases: New members, new pathwaysJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 2 2005Elena M. Sorokina Abstract Proteins comprising the Rho family of GTPases mediate reorganization of the actin cytoskeleton as well as transcription of genes. Recent findings from genome sequencing efforts, genetic screens, and signal transduction research have revealed that the Rho family contains several new, hitherto unrecognized members. In this review, we focus on these newly discovered Rho-GTPases and discuss their role in signaling to the cytoskeleton and the nucleus. © 2004 Wiley-Liss, Inc. [source] Swift,and,Erie: The Trials of an Ephemeral Landmark CaseJOURNAL OF SUPREME COURT HISTORY, Issue 3 2009TONY A. FREYER Like jazz improvisation, the meaning of,Swift v. Tyson,was elusive.1 Justice Joseph Story's 1842 opinion concerning an important commercial-law issue arose from a jury trial.2 When the creditor plaintiff appealed, counsel for the winning debtor raised as a defense Section 34 of the 1789 Judiciary Act. The federal circuit court disagreed about the standing of commercial law under Section 34. Although profound conflicts otherwise divided nationalist and states'-rights proponents, the Supreme Court endorsed Story's commercial-law opinion unanimously.3 New members of the Court and the increasing number of federal lower-court judges steadily transformed the,Swift,doctrine; after the Civil War it agitated the federal judiciary, elite lawyers, and Congress.4 Asserting contrary tenets of American constitutionalism, the Supreme Court overturned the ninety-six-year-old precedent in,Erie Railroad v. Tompkins,(1938).5 The,Swift,doctrine's resonance with changing times was forgotten. The Court and the legal profession established, transformed, and abandoned the doctrine though an adversarial process and judicial instrumentalism. Although the policy of each decision reflected its time, Story's opinion was more consistent with the federalism of the early Constitution than was,Erie.6 [source] New members of the Editorial BoardCOLORECTAL DISEASE, Issue 2 2003Article first published online: 13 MAR 200 No abstract is available for this article. [source] Expression of qBrn-1, a new member of the POU gene family, in the early developing nervous system and embryonic kidneyDEVELOPMENTAL DYNAMICS, Issue 4 2006Lei Lan Abstract It has been shown that POU domain genes play critical roles in the development of the nervous system. We have obtained a new member of the class III POU domain genes, qBrn-1, from the cDNA library of embryonic day 5 quail and have made an extensive expression pattern analysis of qBrn-1 and qBrn-2 throughout the early embryonic development by in situ hybridization. With a specific antibody we prepared, further analysis by immunohistochemistry showed that the location of qBrn-1 protein was consistent with that of the transcripts in the early developing quail. Our results showed that both qBrn-1 and qBrn-2 were preferentially expressed in the developing central nervous system, and their transcripts were initially detected in the neural plate and later in the distinct regions of the neural tube with a stage-dependent pattern. Moreover, their expression was also detected in both notochord and neural crests. However, qBrn-1 signal, different from qBrn-2, was more widely found in the auditory pits, branchial arches, and in the mesodermal components of the developing kidney. And the expression of qBrn-1 in nephric region was earlier and wider than that of mouse Brn-1, suggesting the characteristic function of qBrn-1 in the kidney formation. The distinct dynamic expression patterns of qBrn-1 and qBrn-2 indicate multiple roles of the class III POU genes in quail neurogenesis and organogenesis. Developmental Dynamics 235:1107,1114, 2006. © 2006 Wiley-Liss, Inc. [source] Isolation and characterization of Tn -Dha1, a transposon containing the tetrachloroethene reductive dehalogenase of Desulfitobacterium hafniense strain TCE1ENVIRONMENTAL MICROBIOLOGY, Issue 1 2005Julien Maillard Summary A new 9.9 kb catabolic transposon, Tn -Dha1, containing the gene responsible for tetrachloroethene (PCE) reductive dechlorination activity, was isolated from Desulfitobacterium hafniense strain TCE1. Two fully identical copies of the insertion sequence ISDha1, a new member of the IS256 family, surround the gene cluster pceABCT, a truncated gene for another transposase and a short open reading frame with homology to a member of the twin-arginine transport system (tatA). Evidence was obtained by Southern blot for an alternative form of the transposon element as a circular molecule containing only one copy of ISDha1. This latter structure most probably represents a dead-end product of the transposition of Tn -Dha1. Strong indications for the transposition activity of ISDha1 were given by polymerase chain reaction (PCR) amplification and sequencing of the intervening sequence located between both inverted repeats (IR) of ISDha1 (IR junction). A stable genomic ISDha1 tandem was excluded by quantitative real-time PCR. Promoter mapping of the pceA gene, encoding the reductive dehalogenase, revealed the presence of a strong promoter partially encoded in the right inverted repeat of ISDha1. A sequence comparison with pce gene clusters from Desulfitobacterium sp. strains PCE-S and Y51 and from Dehalobacter restrictus, all of which show 100% identity for the pceAB genes, indicated that both Desulfitobacterium strains seem to possess the same transposon structure, whereas only the pceABCT gene cluster is conserved in D. restrictus. [source] The role of the ICOS/B7RP-1 T cell costimulatory pathway in murine experimental autoimmune uveoretinitisEUROPEAN JOURNAL OF IMMUNOLOGY, Issue 11 2006Yoshihiko Usui Abstract ICOS/B7RP-1 is a new member of the CD28/B7 family of costimulatory molecules and plays differential roles in autoimmune diseases. In this study, we examined the role of ICOS/B7RP-1 pathway in the pathogenesis of mouse experimental autoimmune uveoretinitis (EAU), an animal model of human autoimmune uveitis. ICOS expression was found on infiltrating CD4+ T cells in the region of the retina in EAU-induced mice. The anti-B7RP-1 monoclonal antibody (mAb)-treated or ICOS-deficient mice showed a substantial reduction of disease scores. Blockade of ICOS/B7RP-1 interaction during the effector phase ameliorated the disease, whereas its blockade during the induction phase exhibited no significant effect. Moreover, administration of anti-B7RP-1 mAb effectively ameliorated the disease induced by adoptive transfer of pathogenic T cells. The anti-B7RP-1 mAb treatment inhibited the expansion and/or effector function of pathogenic T cells, given that proliferative response and IFN-, production by lymph node cells were reduced upon restimulation with the antigen peptide in vitro. These results suggest that the ICOS/B7RP-1 interaction plays a critical role in the pathogenesis of uveitis. We also indicated that ICOS-mediated costimulation plays differential roles in EAU and experimental autoimmune encephalomyelitis, which is also a Th1 disease induced in the same manner as EAU. [source] Solvothermal Synthesis, Crystal Structure, and Thermal Stability of Three-Layered Thioantimonate(III) Complexes: [Ni(C3H10N2)3]Sb4S7, [C4H14N2]Sb8S13·H2O, and [C6H18N2]Sb10S16·H2OEUROPEAN JOURNAL OF INORGANIC CHEMISTRY, Issue 11 2007Meng Zhang Abstract Three new thioantimonate(III) complexes [Ni(1,2-PDA)3]Sb4S7 (1) (1,2-PDA = 1,2-propanediamine), [dmenH22+]Sb8S13·H2O (2) (dmen = N,N -dimethylethylenediamine), and [deenH22+]Sb10S16·H2O (3) (deen = N,N -diethylethylenediamine), prepared under solvothermal conditions, have been characterized by single-crystal X-ray diffraction, elemental analysis, and DTA-TG measurements. In compound 1, a rectangle-like Sb16S16 heteroring whose dimensions are about 8.1,×,14.7 Å is observed, this is the largest reported pore in layered thioantimonates to date. Further condensation of the Sb16S16 heterorings resulted in an unprecedented framework of the five-atom thick SbxSyn, layer. In 2, a new member of SbnSn (n = 2, 3, 4, 8, 16, 30, 31, 32) heterocycles Sb15S15 heteroring has been obtained as the first example. In compound 3, the most particular feature is that when the secondary Sb,S bonds are considered the Sb2 atom becomes sevenfold coordinated.(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007) [source] Molecular cloning and expression regulation of PRG-3, a new member of the plasticity-related gene familyEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 1 2004Nicolai E. Savaskan Abstract Phospholipid-mediated signalling on neurons provokes diverse responses such as neurogenesis, pattern formation and neurite remodelling. We have recently uncovered a novel set of molecules in the mammalian brain, named plasticity-related genes (PRGs), which mediate lipid phosphate phosphatase activity and provide evidence for their involvement in mechanisms of neuronal plasticity. Here, we report on a new member of the vertebrate-specific PRG family, which we have named plasticity-related gene-3 (PRG-3). PRG-3 is heavily expressed in the brain and shows a specific expression pattern during brain development where PRG-3 expression is found predominantly in neuronal cell layers and is already expressed at embryonic day 16. In the mature brain, strongest PRG-3 expression occurs in the hippocampus and cerebellum. Overexcitation of neurons induced by kainic acid leads to a transient down-regulation of PRG-3. Furthermore, PRG-3 is expressed on neurite extensions and promotes neurite growth and a spreading-like cell body in neuronal cells and COS-7 cells. In contrast to previously described members of the PRG family, PRG-3 does not perform its function through enzymatic phospholipid degradation. In summary, our findings feature a new member of the PRG family which shows dynamic expression regulation during brain development and neuronal excitation. [source] Analytical modelling of users' behaviour and performance metrics in key distribution schemesEUROPEAN TRANSACTIONS ON TELECOMMUNICATIONS, Issue 1 2010Massimo Tornatore Access control for group communications must ensure that only legitimate users can access the authorised data streams. This could be done by distributing an encrypting key to each member of the group to be secured. To achieve a high level of security, the group key should be changed every time a user joins or leaves the group, so that a former group member has no access to current communications and a new member has no access to previous communications. Since group memberships could be very dynamic, the group key should be changed frequently. So far, different schemes for efficient key distribution have been proposed to limit the key-distribution overhead. In previous works, the performance comparison among these different schemes have been based on simulative experiments, where users join and leave secure groups according to a basic statistical model of users' behaviour. In this paper, we propose a new statistical model to account for the behaviour of users and compare it to the modelling approach so far adopted in the literature. Our new model is able to to lead the system to a steady state (allowing a superior statistical confidence of the results), as opposed to current models in which the system is permanently in a transient and diverging state. We also provide analytical formulations of the main performance metrics usually adopted to evaluate key distribution systems, such as rekey overheads and storage overheads. Then, we validate our simulative outcomes with results obtained by analytical formulations. Copyright © 2009 John Wiley & Sons, Ltd. [source] The C-terminus of viral vascular endothelial growth factor-E partially blocks binding to VEGF receptor-1FEBS JOURNAL, Issue 1 2008Marie K. Inder Vascular endothelial growth factor (VEGF) family members play important roles in embryonic development and angiogenesis during wound healing and in pathological conditions such as tumor formation. Parapoxviruses express a new member of the VEGF family which is a functional mitogen that specifically activates VEGF receptor (VEGFR)-2 but not VEGFR-1. In this study, we show that deletion from the viral VEGF of a unique C-terminal region increases both VEGFR-1 binding and VEGFR-1-mediated monocyte migration. Enzymatic removal of O -linked glycosylation from the C-terminus also increased VEGFR-1 binding and migration of THP-1 monocytes indicating that both the C-terminal residues and O -linked sugars contribute to blocking viral VEGF binding to VEGFR-1. The data suggest that conservation of the C-terminal residues throughout the viral VEGF subfamily may represent a means of reducing the immunostimulatory activities associated with VEGFR-1 activation while maintaining the ability to induce angiogenesis via VEGFR-2. [source] Section Neurocentrae Bunge from genus Cousinia Cass. (Asteraceae) in IranFEDDES REPERTORIUM, Issue 1-2 2009S. B. Djavadi This paper is based on experience from field studies, herbarium materials and images of type specimens belonging to sect. Neurocentrae Bunge from genus Cousinia Cass. Section Leucocaulon Tscherneva is considered as synonym of sect. Neurocentrae Bunge, according to the morphological characters of C. turcomanica C.Winkl. (the type specimen of sect. Leucocaulon) and close affinities that shows with the species of sect. Neurocentrae. Section Ramosissimae is described as a section new to science. Differences between sect. Neurocentrae and sect. Ramosissimae are discussed and a key to the species of the two sections are provided separately. Besides, C. deserti var. longispinosa is described as a new member for the sect. Neurocentrae Bunge. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) Die Sektion Neurocentrae Bunge der Gattung Cousinia Cass. (Asteraceae) in Iran Diese Arbeit basiert auf Erfahrungen aus Geländearbeit, Studien an Herbar-Material und Untersuchungen an Typus-Material der Sektion Neurocentrae Bunge der Gattung Cousinia Cass. Die Sektion Leucocaulon Tscherneva wird als Synonym zur Sekt. Neurocentrae betrachtet und zwar auf Grund der morphologischen Merkmale von C. turcomania C.Winkl. (Typus-Art der Sekt. Leucocaulon) und der engen Verwandtschaft dieser Art zur Sektion Neurocentrae. Die Sekt. Ramosissimae wird hier als neue Sektion beschrieben. Die Unterschiede zwischen der Sekt. Neurocentrae und der Sekt. Ramosissimae werden betrachtet und ein Schlüssel für beide Sektionen beigefügt. Außerdem wird Cousinia deserti var. longispinosa als neue Art in der Sektion Neurocentrae beschrieben. [source] Immulectin-4 from the tobacco hornworm Manduca sexta binds to lipopolysaccharide and lipoteichoic acidINSECT MOLECULAR BIOLOGY, Issue 2 2006X.-Q. Yu Abstract Insect C-type lectins function as pattern recognition receptors in innate immunity. In the tobacco hornworm Manduca sexta, we have previously isolated three C-type lectins named immulectins, which are involved in innate immune responses. Here, we report a new member of the immulectin family, immulectin-4 (IML-4). IML-4 mRNA was detected in the fat body of control larvae and was induced in the fat body when larvae were injected with bacteria. Recombinant IML-4 bound to bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and the binding activity was not affected by addition of calcium or EGTA. IML-4 agglutinated bacteria and yeast, and agglutination of Escherichia coli by IML-4 was concentration- and calcium-dependent. IML-4 also enhanced haemocyte encapsulation and melanization. [source] The Old-Boy Network and the Young-Gun EffectINTERNATIONAL ECONOMIC REVIEW, Issue 4 2000Curtis R. Taylor A model of an exclusive group or class whose membership is governed by personal contact and interaction is studied. Members of this old-boy network attempt to shield themselves from transacting with opportunistic or incompetent individuals by dealing only infrequently with unproven nonmembers. This injures the unproven but qualified agents not in the network. Moreover, because recruitment of a new member creates a public good for network members, too little recruiting is performed in equilibrium. [source] Blind separation of convolutive mixtures of cyclostationary signalsINTERNATIONAL JOURNAL OF ADAPTIVE CONTROL AND SIGNAL PROCESSING, Issue 3 2004Wenwu Wang Abstract An adaptive blind source separation algorithm for the separation of convolutive mixtures of cyclostationary signals is proposed. The algorithm is derived by applying natural gradient iterative learning to a novel cost function which is defined according to the wide sense cyclostationarity of signals and can be deemed as a new member of the family of natural gradient algorithms for convolutive mixtures. A method based on estimating the cycle frequencies required for practical implementation of the proposed algorithm is presented. The efficiency of the algorithm is supported by simulations, which show that the proposed algorithm has improved performance for the separation of convolved cyclostationary signals in terms of convergence speed and waveform similarity measurement, as compared to the conventional natural gradient algorithm for convolutive mixtures. Copyright © 2004 John Wiley & Sons, Ltd. [source] ,-Fe2O3 Nanorings Prepared by a Microwave-Assisted Hydrothermal Process and Their Sensing Properties,ADVANCED MATERIALS, Issue 17 2007X. Hu Free-standing , -Fe2O3nanorings are synthesized in solution through a rapid microwave-assisted hydrothermal process. The ringlike structure is a new member in the family of iron oxide nanostructures. The sensors made of the ,-Fe2O3 nanorings exhibit high sensitivity not only for bio-sensing of hydrogen peroxide in a physiological solution but also for gas-sensing of alcohol vapor at room temperature. [source] Evaluation of coexistence of the Human Herpesvirus type 8 (HHV-8) infection and pemphigusINTERNATIONAL JOURNAL OF DERMATOLOGY, Issue 7 2010Naser Tayyebi Meibodi Associate Professor Background, Human Herpesvirus type 8 (HHV-8) is a new member of the herpes virus family, first found in the tissue of acquired immune deficiency syndrome (AIDS)-related Kaposi's sarcoma. Environmental factors including viral infection may play a role in the onset and/or development of pemphigus. Some studies based on polymerase chain reaction findings suggest an association between HHV-8 and pemphigus. The aim of this study is investigation of the association of pemphigus with HHV-8 and the relationship between inflammatory and acantholytic cells with HHV-8 infection. Methods, Tissue-extracted DNA from 41 paraffin-embedded skin tissues from patients first presenting with pemphigus was tested using nested PCR for the presence of HHV-8. A total of 37 cases had pemphigus vulgaris (PV) and 4 patients had pemphigus foliaceus (PF). For our control group, normal skin of 21 cosmetic surgery patients were included. Furthermore, microscopic slides with H&E staining were evaluated for the number of inflammatory and acantholytic cells per microscopic field. Results, Skin lesions from 13 of 37 patients (35.1%) with PV and 2 of 4 cases (50%) with PF were positive for HHV-8 DNA. All specimens in our control group were negative for this virus. Conclusion, HHV-8 infection might be a contributing factor in the initiation or development of pemphigus. [source] Two C-Terminal Variants of NBC4, a New Member of the Sodium Bicarbonate Cotransporter Family: Cloning, Characterization, and LocalizationIUBMB LIFE, Issue 1 2000Alexander Pushkin Abstract We report the cloning, characterization, and chromosomal assignment of a new member of the sodium bicarbonate cotransporter (NBC) family, NBC4. The NBC4 gene was mapped to chromosome 2p13 and is a new candidate gene for Alstrom syndrome. Two variants of the transporter have been isolated from human testis and heart, which differ in their C termini. NBC4a encodes a 1137-residue polypeptide and is widely expressed in various tissues, including liver, testis, and spleen. NBC4b is identical to NBC4a except that it has a 16-nucleotide insert, creating a C-terminal frame shift. NBC4b encodes a 1074-residue polypeptide and is highly expressed in heart. Amino acids 1-1046 are common to both NBC4 variants. NBC4a has two protein-interacting domains that are lacking in NBC4b: a proline-rich sequence, PPPSVIKIP (amino acids 1102-1110), and a consensus PDZ-interacting domain, SYSL (1134-1137). NBC4b lacks the stretch of charged residues present in the C terminus of NBC4a and other members of the NBC family.Unlike other members of the NBC family, both NBC4a and NBC4b have a unique glycine-rich region (amino acids 440- 469). In comparison with other members of the bicarbonate transport superfamily, NBC4a and NBC4b are most similar structurally to the electrogenic sodium bicarbonate cotransporters (NBC1). [source] Antilisterial activity of lactic acid bacteria isolated from rigouta, a traditional Tunisian cheeseJOURNAL OF APPLIED MICROBIOLOGY, Issue 3 2004T. Ghrairi Abstract Aims:, Screening for lactic acid bacteria (LAB) producing bacteriocins and other antimicrobial compounds is of a great significance for the dairy industry to improve food safety. Methods and Results:, Six-hundred strains of LAB isolated from ,rigouta', a Tunisian fermented cheese, were tested for antilisterial activity. Eight bacteriocinogenic strains were selected and analysed. Seven of these strains were identified as Lactococcus lactis and produced nisin Z as demonstrated by mass spectrometry analysis of the purified antibacterial compound. Polymerase chain reaction experiments using nisin gene-specific primers confirmed the presence of nisin operon. Plasmid profiles analysis suggests the presence of, at least, three different strains in this group. MMT05, the eighth strain of this antilisterial collection was identified, at molecular level, as Enterococcus faecalis. The purified bacteriocin produced by this strain showed a molecular mass of 10 201·33 ± 0·85 Da. This new member of class III bacteriocins was termed enterocin MMT05. Conclusions:, Seven lactococcal strains producing nisin Z were selected and could be useful as bio-preservative starter cultures. Additional experiments are needed to evaluate the promising strain MMT05 as bio-preservative as Enterococci could exert detrimental or beneficial role in foods. Significance and Impact of the Study:, Only a few antibacterial strains isolated from traditional African dairy products were described. The new eight strains described herein contribute to the knowledge of this poorly studied environment and constitute promising strains for fermented food safety. [source] Alphavirus infections in salmonids , a reviewJOURNAL OF FISH DISEASES, Issue 9 2007M F McLoughlin Abstract The first alphavirus to be isolated from fish was recorded in 1995 with the isolation of salmon pancreas disease virus from Atlantic salmon, Salmo salar L., in Ireland. Subsequently, the closely related sleeping disease virus was isolated from rainbow trout, Oncorhynchus mykiss (Walbaum), in France. More recently Norwegian salmonid alphavirus (SAV) has been isolated from marine phase production of Atlantic salmon and rainbow trout in Norway. These three viruses are closely related and are now considered to represent three subtypes of SAV, a new member of the genus Alphavirus within the family Togaviridae. SAVs are recognized as serious pathogens of farmed Atlantic salmon and rainbow trout in Europe. This paper aims to draw together both historical and current knowledge of the diseases caused by SAVs, the viruses, their diagnosis and control, and to discuss the differential diagnosis of similar pathologies seen in cardiomyopathy syndrome and heart and skeletal muscle inflammation of Atlantic salmon. [source] | ||||||||||||||||||||||||||||||||||