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Necessary Procedure (necessary + procedure)
Selected AbstractsIdentification and Characterization of an Organic Solvent Tolerance Gene in Helicobacter pyloriHELICOBACTER, Issue 1 2007Hung-Chuan Chiu Abstract Background:, Pre-cleaning and soaking in glutaraldehyde is the necessary procedure to disinfect endoscopes. However, some chemical-solvent-tolerant bacteria may survive after incomplete endoscopic disinfection. The goal of this study was to identify glutaraldehyde resistance-related genes in Helicobacter pylori. Materials and Methods:, ,-Zap phagemid expression library of H. pylori strain NTUH-C1 was selected with 0.1% glutaraldehyde. The minimal inhibitory concentration (MIC) of glutaraldehyde-resistant DNA fragments of H. pylori NTUH-C1 strain were determined. Imp/OstA recombinant protein was expressed, purified, and used to generate anti-Imp/OstA polyclonal antibody. Imp/ostA knockout, deletion, and complementation strains were constructed. The function of Imp/OstA was monitored by organic solvent tolerance assay, antibiotics susceptibility test, and n -phenylnapthylamine assay. Results:, Using Imp/ostA polyclonal antibody against cell lysate of wild-type and imp/ostA mutant showed that it is not essential in H. pylori. Organic solvent tolerance assay demonstrated the role of Imp/ostA in n-hexane tolerance. MIC test showed that the mutation of imp/ostA was susceptible to hydrophobic and ,-lactam antibiotics. NPN assay demonstrated that the level of outer membrane permeability was increased by 50% in mutant strain comparing to wild-type strain (p < .001). Conclusions:, We have identified an Imp/OstA protein that was associated with glutaraldehyde resistance in our clinical strain H. pylori NTUH-C1 by screening of ,-Zap expression library. Disruption of this protein results in altering membrane permeability, sensitivity to organic solvent, and susceptibility to antibiotics. [source] The lateral X-ray of the hip in fracture: a necessary procedure?JOURNAL OF EVALUATION IN CLINICAL PRACTICE, Issue 1 2008Amit Sharad Chandra Bidwai MBChB Senior House Officer in General Surgery [source] Strain E26 of Agrobacterium vitis, a Biological Control Agent of Grapevine Crown Gall, Does Not Contain virA and virG Pathogenic DeterminantsJOURNAL OF PHYTOPATHOLOGY, Issue 11-12 2009Qing Wei Abstract Risk assessment of biological control agents (BCAs) for the control of plant diseases in the field and/or laboratories has now become a necessary procedure before developing and producing novel BCAs. Agrobacterium vitis strain E26 is a promising potential biocontrol agent of grapevine crown gall disease. However, much less is understood about its safety or environmental risks. In this study, polymerase chain reaction (PCR) and Southern blot analyses were used to determine whether five essential virulence genes (virA, virG, iaaH, iaaM and ipt) were present in strain E26. Primers and probes were designed based on the conserved regions of each gene. The overall results obtained indicated that A. vitis strain E26 does not contain the virA and virG determinants, suggesting that this strain would be unlikely to elicit crown gall symptoms in either host or non-host plants. It seems that the iaaH, iaaM, or ipt gene were not present in strain E26 either. An applicable new approach combining PCR and Southern blot analyses to examine the pathogenicity of potential BCAs, particularly BCAs from the genus of Agrobacterium spp. was described. [source] Review of recommendations for the management of dental trauma presented in first-aid textbooks and manualsDENTAL TRAUMATOLOGY, Issue 3 2010Katarzyna Emerich Proper information in first-aid textbooks and manuals should be the best way to present necessary procedures on how to act at the site of injury. The objective of this review is to report the quality of the knowledge presented in first-aid books and manuals. Methods:, We carried out a review of first-aid international textbooks and manuals available in Medical University Libraries in Poland. The inclusion criteria were all manuals on first-aid that were written for medical staff and lay persons, and were published between 1969 and 2007. All texts were screened for dental trauma treatment recommendations. Results:, Our literature review has shown that among 45 first-aid textbooks and manuals only 19 mention procedures for use in case of dental trauma. Of those texts, only 13 detail the storage media for an avulsed tooth until replantation. Conclusions:, Current, evidence-based, recommendations concerning first-aid procedures after dental trauma should be incorporated in forthcoming editions of first-aid textbooks and manuals. The guidance on procedures contained in reviewed texts is misleading. [source] Patient Injuries from Surgical Procedures Performed in Medical Offices: Three Years of Florida DataDERMATOLOGIC SURGERY, Issue 12p1 2004Brett Coldiron MD, FACP Background. Many state medical boards and legislatures are in the process of developing regulations that restrict procedures in the office setting with the intention of enhancing patient safety. The highest quality data in existence on office procedure adverse incidents have been collected by the state of Florida. Objective. The objective was to determine and analyze the nature of surgical incidents in office-based settings using 3 years of Florida data from March 2000 to March 2003. Methods. An incidence study with prospective data collection was performed. Individual reports that resulted in death or a hospital transfer were further investigated by determining the reporting physician's board certification status, hospital privilege status (excluding procedure specific operating room privileges), and office accreditation status. Results. In 3 years there were 13 procedure-related deaths and 43 procedure-related complications that resulted in a hospital transfer. Seven of the 13 deaths involved elective cosmetic procedures, 5 of which were performed under general anesthesia and 2 of which were performed with intravenous sedation anesthesia. Forty-two percent of the offices reporting deaths and 50% of the offices reporting procedural incidents that resulted in a hospital transfer were accredited by an independent accreditation agency. Ninety-six percent of physicians reporting surgical incidents were board-certified, and all had hospital privileges. Conclusions. Restrictions on office procedures for medically necessary procedures, such as requiring office accreditation, board certification, and hospital privileges, would have little effect on overall safety of surgical procedures. These data also show that the greatest danger to patients lies not with surgical procedures in office-based settings per se, but with cosmetic procedures that are performed in office-based settings, particularly when under general anesthesia. Our conclusions are dramatically different from those of a recent study, which claimed a 12-fold increased risk of death for procedures in the office setting. [source] | ||||||||||