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Naked Amoebae (naked + amoeba)

Distribution by Scientific Domains
Life Sciences100%

Selected Abstracts

High Numbers of Naked Amoebae in the Planktonic Waters of a Mangrove Stand in Southern Florida, USA

THE JOURNAL OF EUKARYOTIC MICROBIOLOGY, Issue 3 2000
ANDREW ROGERSON
ABSTRACT. This is the first study to examine the abundance of naked amoebae in the water column of a mangrove stand. A total of 37 different morphotypes was noted and at least 13 of these are probably new species. Over a one-year sampling interval, amoebae averaged 35,400 cells liter,1 (range 2,000,104,000) by an indirect enrichment cultivation method. Densities in the upper end of this range arc the highest ever reported for any planktonic habitat. Variation between samples was related to the quantity of suspended aggregates (floes) in the water column emphasizing that amoebae are usually floc-associated. The study also showed that it is essential to disrupt floc material prior to withdrawing sample aliquots for the indirect counting method since several amoebae can occupy the interstices of aggregates. There is concern that indirect enumeration methods that require organisms to be cultured in the laboratory seriously underestimate the true count. A direct counting method using acridine orange staining and epifluorescence microscopy was attempted to assess the possible magnitude of the error associated with indirect counting. While this direct method had limitations, notably the difficulty of unambiguously differentiating between small amoebae and nanoflagellates, the results suggested that the indirect method gave estimates that were close to the true count (within a factor of two). Mangrove waters are rich in heterotrophic protozoa (,3 × 106 liter1) and while the heterotrophic flagellates are by far the dominant group, naked amoebae outnumber ciliates some 20-foid. The ecological consequences of high numbers of amoebae, particularly the common small forms less than 10 ,m in length, need to be examined for these important coastal sites. [source]

A methane-driven microbial food web in a wetland rice soil

ENVIRONMENTAL MICROBIOLOGY, Issue 12 2007
Jun Murase
Summary Methane oxidation is a key process controlling methane emission from anoxic habitats into the atmosphere. Methanotrophs, responsible for aerobic methane oxidation, do not only oxidize but also assimilate methane. Once assimilated, methane carbon may be utilized by other organisms. Here we report on a microbial food web in a rice field soil driven by methane. A thin layer of water-saturated rice field soil was incubated under opposing gradients of oxygen and 13C-labelled methane. Bacterial and eukaryotic communities incorporating methane carbon were analysed by RNA-stable isotope probing (SIP). Terminal restriction fragment length polymorphism (T-RFLP) and cloning showed that methanotrophs were the most prominent group of bacteria incorporating methane carbon. In addition, a few Myxobacteria -related sequences were obtained from the ,heavy' rRNA fraction. Denaturing gradient gel electrophoresis (DGGE) targeting 18S rRNA detected various groups of protists in the ,heavy' rRNA fraction including naked amoeba (Lobosea and Heterolobosea), ciliates (Colpodea) and flagellates (Cercozoa). Incubation of soil under different methane concentrations in air resulted in the development of distinct protozoan communities. These results suggest that methane carbon is incorporated into non-methanotrophic pro- and microeukaryotes probably via grazing, and that methane oxidation is a shaping force of the microeukaryotic community depending on methane availability. [source]

The Role of Amoeboid Protists and the Microbial Community in Moss-Rich Terrestrial Ecosystems: Biogeochemical Implications for the Carbon Budget and Carbon Cycle, Especially at Higher Latitudes,

THE JOURNAL OF EUKARYOTIC MICROBIOLOGY, Issue 3 2008
O. ROGER ANDERSON
ABSTRACT. Moss-rich terrestrial communities are widely distributed in low- and high-latitude environments, covering vast surface areas in the boreal forests and tundra. The microbial biota in these organic-rich communities may contribute substantially to the carbon budget of terrestrial communities and the carbon cycle on a global scale. Recent research is reported on the carbon content of microbial communities in some temperate and high-latitude moss communities. The total carbon content and potential respiratory carbon dioxide (CO2) efflux is reported for bacteria, microflagellates, naked amoebae, and testate amoebae within sampling sites at a northeastern forest and the tundra at Toolik, Alaska. Quantitative models of the predicted total CO2 efflux from the microbes, based on microscopic observations and enumeration of the microbiota in samples from the research sites, are described and predictions are compared with published field-based data of CO2 efflux. The significance of the predictions for climate change and global warming are discussed. [source]

High Numbers of Naked Amoebae in the Planktonic Waters of a Mangrove Stand in Southern Florida, USA

THE JOURNAL OF EUKARYOTIC MICROBIOLOGY, Issue 3 2000
ANDREW ROGERSON
ABSTRACT. This is the first study to examine the abundance of naked amoebae in the water column of a mangrove stand. A total of 37 different morphotypes was noted and at least 13 of these are probably new species. Over a one-year sampling interval, amoebae averaged 35,400 cells liter,1 (range 2,000,104,000) by an indirect enrichment cultivation method. Densities in the upper end of this range arc the highest ever reported for any planktonic habitat. Variation between samples was related to the quantity of suspended aggregates (floes) in the water column emphasizing that amoebae are usually floc-associated. The study also showed that it is essential to disrupt floc material prior to withdrawing sample aliquots for the indirect counting method since several amoebae can occupy the interstices of aggregates. There is concern that indirect enumeration methods that require organisms to be cultured in the laboratory seriously underestimate the true count. A direct counting method using acridine orange staining and epifluorescence microscopy was attempted to assess the possible magnitude of the error associated with indirect counting. While this direct method had limitations, notably the difficulty of unambiguously differentiating between small amoebae and nanoflagellates, the results suggested that the indirect method gave estimates that were close to the true count (within a factor of two). Mangrove waters are rich in heterotrophic protozoa (,3 × 106 liter1) and while the heterotrophic flagellates are by far the dominant group, naked amoebae outnumber ciliates some 20-foid. The ecological consequences of high numbers of amoebae, particularly the common small forms less than 10 ,m in length, need to be examined for these important coastal sites. [source]