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N Sources (n + source)
Selected AbstractsPhenotypical variation in a toxic strain of the phytoplankter, Cylindrospermopsis raciborskii (nostocales, cyanophyceae) during batch cultureENVIRONMENTAL TOXICOLOGY, Issue 6 2001Peter R. Hawkins Abstract A nonaxenic strain of Cylindrospermopsis raciborskii Woloszynska (AWT 205) was grown in batch culture, with and without nitrate as the primary N source. Rapid log-phase growth with nitrate was 1.0 doubling/day versus 0.3 doubling/day without nitrate. Cylindrospermopsin (CYN) production was measured by HPLC. The rate of intracellular CYN production matched cell division rate for both the diazotrophies at cell densities less than 107 cell/ml. At cell density >107 cell/ml, additional resource limitation in batch culture slowed log-phase growth to 0.04 division/day and cell division and CYN production decoupled. Intracellular CYN concentration increased at a rate of 0.08 doubling/day, twice the cell division rate. Extracellular CYN as a proportion of the total CYN increased from 20% during the rapid growth phase, to 50% during the slow growth phase. The total CYN yield from cultures grown out to stationary phase (55 days) exceeded 2 mg CYN/l. C. raciborskii cells in log-phase growth, exposed to 1 ppm copper (as copper sulphate), lysed within 24 hours. After copper treatment, all CYN was in the filterable fraction. These findings imply that in naturally occurring blooms of C. raciborskii, the movement of intracellular CYN into solution will be the greatest during stationary phase, when intracellular concentrations are highest and cell lysis is more frequent. The application of algicides that promote cell lysis will exacerbate this effect. © 2001 John Wiley & Sons, Inc. Environ Toxicol 16: 460,467, 2001 [source] A quantitative review comparing the yield of switchgrass in monocultures and mixtures in relation to climate and management factorsGCB BIOENERGY, Issue 1 2010DAN WANG Abstract Switchgrass (Panicum virgatum L.), a US Department of Energy model species, is widely considered for US biomass energy production. While previous studies have demonstrated the effect of climate and management factors on biomass yield and chemical characteristics of switchgrass monocultures, information is lacking on the yield of switchgrass grown in combination with other species for biomass energy. Therefore, the objective of this quantitative review is to compare the effect of climate and management factors on the yield of switchgrass monocultures, as well as on mixtures of switchgrass, and other species. We examined all peer-reviewed articles describing productivity of switchgrass and extracted dry matter yields, stand age, nitrogen fertilization (N), temperature (growing degree days), and precipitation/irrigation. Switchgrass yield was greater when grown in monocultures (10.9 t ha,1, n=324) than when grown in mixtures (4.4 t ha,1, n=85); yield in monocultures was also greater than the total yield of all species in the mixtures (6.9 t ha,1, n=90). The presence of legume species in mixtures increased switchgrass yield from 3.1 t ha,1 (n=65) to 8.9 t ha,1 (n=20). Total yield of switchgrass-dominated mixtures with legumes reached 9.9 t ha,1 (n=25), which was not significantly different from the monoculture yield. The results demonstrated the potential of switchgrass for use as a biomass energy crop in both monocultures and mixtures across a wide geographic range. Monocultures, but not mixtures, showed a significant positive response to N and precipitation. The response to N for monocultures was consistent for newly established (stand age <3 years) and mature stands (stand age ,3 years) and for lowland and upland ecotypes. In conclusion, these results suggest that fertilization with N will increase yield in monocultures, but not mixtures. For monocultures, N treatment need not be changed based on ecotype and stand age; and for mixtures, legumes should be included as an alternative N source. [source] Effects of Salinity and Mixed Ammonium and Nitrate Nutrition on the Growth and Nitrogen Utilization of BarleyJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 4 2001A. Ali The absorption and utilization of nitrogen (N) by plants are affected by salinity and the form of N in the root medium. A hydroponic study was conducted under controlled conditions to investigate growth and N uptake by barley (Hordeum vulgare L.) supplied with five different NH4+ -N/NO3, -N ratios at electrical conductivity of 0 and 8 dS m,1. The five NH4+ -N/NO3 -N ratios were 0/100, 25/75, 50/50, 75/25 and 100/0, each giving a total N supply of 100 mg N l,1 in the root medium. A mixed N supply of NH4+ and NO3, resulted in greater accumulation of N in plants than either NO3, or NH4+ as the sole N source. Plants produced a significantly higher dry matter yield when grown with mixed N nutrition than with NH4+ or NO3, alone. Total dry matter production and root and shoot N contents decreased with increasing salinity in the root medium. The interaction between salinity and N nutrition was found to be significant for all the variables. A significant positive correlation (r=0.97) was found between nitrogen level in the plant shoot and its dry matter yield. Wachstum und Stickstoffausnutzung bei Gerste in Abhängigkeit von Versalzung und Michungen von Ammonium und Nitrat Aufnahme und Nutzung von N durch Pflanzen wird von der Versalzung und N-Form im Wurzelbereich bestimmt. Es wurde in Hydrokultur unter kontrollierten Bedingungen Wachstum und N-Aufnahme durch Gerste (Hordeum vulgare L.) bei Anwendung von fünf unterschiedlichen NH4+ -N/NO3, -N Verhältnissen bei einer elektrischen Konduktivität von 0 und 8 dS m,1 untersucht. Die Gesamtmenge von 100 mg N l,1 im Wurzelmedium wies NH4+ -N/NO3, -N Verhältnisse von 0/100, 25/75, 50/50, 75/25 und 100/0 auf. Mischungen von NH4+ und NO3, führten zu einer größeren Aufnahme durch die Pflanzen als bei alleiniger Anwendung von NO3, oder NH4+. Die Pflanzen produzierten signifikant mehr Gesamttrockenmasse mit Mischungen der beiden N-Formen im Vergleich zu alleiniger Anwendung von NH4+ oder NO3,. Die Gesamttrockenmasse sowie die N-Gehalte von Wurzel und Sproß nahmen mit steigender Versalzung ab. Versalzungs- und N-Versorgungs-Interaktion war signifikant in allen Versuchsbedingungen. Eine signifikante positive Korrelation (r=0,97) wurde zwischen Stickstoffkonzentration und der Trockenmasseproduktion der Pflanze gefunden. [source] Effects of aromatic amino acids, phenylacetate and phenylpropionate on fermentation of xylan by the rumen anaerobic fungi, Neocallimastix frontalis and Piromyces communisJOURNAL OF APPLIED MICROBIOLOGY, Issue 4 2007A.Y. Guliye Abstract Aims:, Anaerobic fungi are important members of the fibrolytic community of the rumen. The aim of this study was to study their requirement for aromatic amino acids (AA) and related phenyl acids (phenylpropionic and phenylacetic acids) for optimal xylan fermentation. Methods and Results:,Neocallimastix frontalis RE1 and Piromyces communis P were grown in a defined medium containing oat spelts xylan as the sole energy source, plus one of the following N sources: ammonia; ammonia plus a complete mixture of 20 AA commonly found in protein; ammonia plus complete AA mixture minus aromatic AA; ammonia plus phenyl acids; ammonia plus complete AA mixture without aromatic AA plus phenyl acids. Both species grew in all the media, indicating no absolute requirement for AA. The complete AA mixture increased (P < 0·05) acetate concentration by 18% and 15%, sugar utilization by 33% and 22% and microbial yield by about 22% and 15% in N. frontalis and P. communis, respectively, in comparison with the treatments that had ammonia as the only N source. Neither the supply of aromatic AA or phenol acids, nor their deletion from the complete AA mixture, affected the fermentation rate, products or yield of either species. Conclusions:, AA were not essential for N. frontalis and P. communis, but their growth on xylan was stimulated. The effects could not be explained in terms of aromatic AA alone. Significance and Impact of the Study:, Ruminant diets should contain sufficient protein to sustain optimal fibre digestion by ruminal fungi. Aromatic AA or phenyl acids alone cannot replace the complete AA mixture. [source] RAPID AMMONIUM- AND NITRATE-INDUCED PERTURBATIONS TO CHL a FLUORESCENCE IN NITROGEN-STRESSED DUNALIELLA TERTIOLECTA (CHLOROPHYTA),JOURNAL OF PHYCOLOGY, Issue 2 2003Erica B. Young When NH4+ or NO3, was supplied to NO3, -stressed cells of the microalga Dunaliella tertiolecta Butcher, immediate transient changes in chl a fluorescence were observed over several minutes that were not seen in N-replete cells. These changes were predominantly due to nonphotochemical fluorescence quenching. Fluorescence changes were accompanied by changes in photosynthetic oxygen evolution, indicating interactions between photosynthesis and N assimilation. The magnitude of the fluorescence change showed a Michaelis-Menten relationship with half-saturation concentration of 0.5 ,M for NO3, and 10 ,M for NH4+. Changes in fluorescence responses were characterized in D. tertiolecta both over 5 days of N starvation and in cells cultured at a range of NO3, -limited growth rates. Variation in responses was more marked in starved than in limited cells. During N starvation, the timing and onset of the fluorescence responses were different for NO3, versus NH4+ and were correlated with changes in maximum N uptake rate during N starvation. In severely N-starved cells, the major fluorescence response to NO3, disappeared, whereas the response to NH4+ persisted. N-starved cells previously grown with NH4+ alone showed fluorescence responses with NH4+ but not NO3, additions. The distinct responses to NO3, and NH4+ may be due to the differences between regulation of the uptake mechanisms for the two N sources during N starvation. This method offers potential for assessing the importance of NO3, or NH4+ as an N source to phytoplankton populations and as a diagnostic tool for N limitation. [source] Does the source of nitrogen affect the response of subterranean clover to prolonged root hypoxia?JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 2 2010Faouzi Horchani Abstract Nitrogen (N) is taken up by most plant species in the form of nitrate (NO) or ammonium (NH). The plant response to continuous ammonium nutrition is species-dependent. In this study, the effects of the source of N nutrition (NO, NH, or the mixture of NO and NH) on the response of clover (Trifolium subterraneum L. cv. 45C) plants to prolonged root hypoxia was studied. Under aerobic conditions, plant growth was strongly depressed by NH, compared to NO or mixed N nutrition, as indicated by the significant decrease in root and shoot-dry-matter production (DW), root and shoot water contents (WC), leaf chlorophyll concentration, and chlorophyll fluorescence parameters (F0, Fv/Fm). However, the N source had no effect on chlorophyll a,to,chlorophyll b ratio. Under hypoxic conditions, the negative effects of root hypoxia on plant-growth parameters (DW and WC), leaf chlorophyll concentration, and chlorophyll fluorescence parameters were alleviated by NH rather than NO supply. Concomitantly, shoot DW,to,root DW ratio, and root and leaf NH concentrations were significantly decreased, whereas root and leaf carbohydrate concentrations, glutamine synthetase activities, and protein concentrations were remarkably increased. The present data reveal that the N source (NO or NH) is a major factor affecting clover responses to hypoxic stress, with plants being more tolerant when NH is the N form used. The different sensitivity is discussed in terms of a competition for energy between nitrogen assimilation and plant growth. [source] Effects of nitrate-, ammonium-, and organic-nitrogen-based fertilizers on growth and yield of tomatoesJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 1 2005Anuschka Heeb Abstract Mineral and organic fertilizers contain different forms and amounts of nitrogen (N), which can affect yield and product quality. The aim of this study was to determine appropriate amounts of N applied as nitrate (NO), ammonium (NH), and organic N (a mixture based on chicken manure) for optimal growth and quality of tomatoes. A pot experiment with sand as substrate was established in a greenhouse with six-week-old tomato plants (Lycopersicon esculentum Mill. cv. "Armada"). Nitrogen was applied in nutrient solutions at different NO : NH ratios combined with different chloride levels (NO -dominated, NO = NH at low Cl,, NO = NH at high Cl,, and NH -dominated, respectively) or as organic N at four N-application rates (250, 500, 750, 1000 mg N plant,1 week,1). No significant differences in shoot biomass and yields of red tomatoes were observed between NO - or NH -fed plants. Nitrogen rates above 750 mg N plant,1 week,1 did not significantly increase marketable fruit yield, but enhanced shoot-biomass production. The NH -N-dominated treatments (which also had high Cl, concentrations) showed increasing incidence of blossom-end-rot (BER)-infected fruits. In the organic-N treatments, shoot-biomass production and yields were lower than in the inorganic-N treatments, but fruit quality was good with few BER-infected fruits. The results show that with a total N supply below 750 mg N plant,1 week,1, NH can be used as equivalent N source to NO, resulting in equivalent yields of marketable fruit under the conditions in this experiment. Einfluss von Nitrat, Ammonium und organischem Stickstoff auf Wachstum und Ertrag von Tomaten Mineralische und organische Dünger enthalten verschiedene Formen von und Mengen an Stickstoff (N), welche den Ernteertrag und die Produktqualität beeinflussen können. Das Ziel dieser Arbeit war es, geeignete N-Mengen , appliziert als Nitrat, Ammonium oder organischer Stickstoff , für optimales Wachstum und Qualität von Tomaten zu bestimmen. Dazu wurde mit sechs Wochen alten Tomatenpflanzen (Lycopersicon esculentum Mill. cv. "Armada") unter Gewächshausbedingungen mit Sand als Substrat ein Gefäßversuch angelegt. Die Applikation von N erfolgte in Form von Nährlösungen mit verschiedenen NO:NH -Verhältnissen, kombiniert mit unterschiedlichen Chlorid (Cl)-Konzentrationen (NO -dominiert, NO = NH bei niedrigem Cl,, NO = NH bei hohem Cl, und NH -dominiert) bzw. als organischer N. Jede dieser Behandlungen wurde mit vier verschiedenen N-Mengen angelegt (250, 500, 750, 1000 mg N Pflanze,1 Woche,1). Zwischen den mit Nitrat und Ammonium gedüngten Pflanzen konnte kein signifikanter Unterschied in Sprosswachstum und Ertrag an erntereifen Tomaten festgestellt werden. Stickstoffmengen über 750 mg N Pflanze,1 Woche,1 steigerten den Ertrag verkaufsfähiger Tomaten nicht signifikant, obwohl die Produktion an Sprossbiomasse noch anstieg. In den NH -dominiert gedüngten Gefäßen mit gleichzeitig hohen Cl-Konzentrationen in den Nährlösungen wurden häufiger Tomaten mit Blütenendfäule beobachtet. In den Behandlungen mit organischem Stickstoff waren die Sprossbiomasseproduktion und der Ertrag geringer als in den mineralisch gedüngten Behandlungen, aber die Qualität der Früchte war gut, mit nur wenigen durch Blütenendfäule geschädigten Tomaten. Die Ergebnisse zeigen, dass bei einer Stickstoffversorgung unter 750 mg N Pflanze,1 Woche,1 Ammonium anstelle von Nitrat verwendet werden kann. Im vorliegenden Versuch wurden unter diesen Bedingungen vergleichbare Erträge verkaufsfähiger Früchte erzielt. [source] Proton release by N2 -fixing plant roots: A possible contribution to phytoremediation of calcareous sodic soilsJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 1 2003Manzoor Qadir Prof. Dr. Abstract With a world-wide occurrence on about 560 million hectares, sodic soils are characterized by the occurrence of excess sodium (Na+) to levels that can adversely affect crop growth and yield. Amelioration of such soils needs a source of calcium (Ca2+) to replace excess Na+ from the cation exchange sites. In addition, adequate levels of Ca2+ in ameliorated soils play a vital role in improving the structural and functional integrity of plant cell walls and membranes. As a low-cost and environmentally feasible strategy, phytoremediation of sodic soils , a plant-based amelioration , has gained increasing interest among scientists and farmers in recent years. Enhanced CO2 partial pressure (PCO2) in the root zone is considered as the principal mechanism contributing to phytoremediation of sodic soils. Aqueous CO2 produces protons (H+) and bicarbonate (HCO3 - ). In a subsequent reaction, H+ reacts with native soil calcite (CaCO3) to provide Ca2+ for Na+ Ca2+ exchange at the cation exchange sites. Another source of H+ may occur in such soils if cropped with N2 -fixing plant species because plants capable of fixing N2 release H+ in the root zone. In a lysimeter experiment on a calcareous sodic soil (pHs = 7.4, electrical conductivity of soil saturated paste extract (ECe) = 3.1 dS m -1, sodium adsorption ratio (SAR) = 28.4, exchangeable sodium percentage (ESP) = 27.6, CaCO3 = 50 g kg -1), we investigated the phytoremediation ability of alfalfa (Medicago sativa L.). There were two cropped treatments: Alfalfa relying on N2 fixation and alfalfa receiving NH4NO3 as mineral N source, respectively. Other treatments were non-cropped, including a control (without an amendment or crop), and soil application of gypsum or sulfuric acid. After two months of cropping, all lysimeters were leached by maintaining a water content at 130% waterholding capacity of the soil after every 24±1 h. The treatment efficiency for Na+ removal in drainage water was in the order: sulfuric acid > gypsum = N2 -fixing alfalfa > NH4NO3-fed alfalfa > control. Both the alfalfa treatments produced statistically similar root and shoot biomass. We attribute better Na+ removal by the N2 -fixing alfalfa treatment to an additional source of H+ in the rhizosphere, which helped to dissolve additional CaCO3 and soil sodicity amelioration. Protonenabgabe durch N2 -fixierende Pflanzenwurzeln: ein möglicher Beitrag zur Phytomelioration von kalkreichen Natriumböden Bei einem weltweiten Vorkommen auf etwa 560 Millionen Hektar sind Natriumböden durch einen Überschuss an Natrium (Na+) gekennzeichnet, der das Wachstum und den Ertrag von Kulturpflanzenbeständen nachteilig beeinflussen kann. Die Melioration solcher Böden erfordert Calcium (Ca2+), um überschüssiges Na+ von Kationen-Austauscherplätzen zu verdrängen. Außerdem spielt Ca2+ eine wichtige Rolle bei der Verbesserung der strukturellen und funktionellen Integrität pflanzlicher Zellwände und Membranen. Als kostengünstige und umweltfreundliche Strategie hat die Phytomelioration von Natriumböden , eine auf Pflanzen beruhende Melioration , in den letzten Jahren zunehmendes Interesse bei Wissenschaftlern und Landwirten gefunden. Ein erhöhter CO2 -Partialdruck (PCO2) in der Rhizosphäre wird als hauptsächlicher Mechanismus angesehen, der zur Phytomelioration von Natriumböden beiträgt. In Wasser gelöst, erzeugt CO2 Protonen (H+) und Bikarbonate (HCO3 - ). Anschließend reagiert H+ mit nativem Calcit (CaCO3), wobei sich Ca2+ löst und Na+ von Austauscherplätzen verdrängt. Eine weitere H+ -Quelle könnte die H+ -Abgabe von Wurzeln N2 -fixierender Pflanzen sein, da diese in der Lage sind, H+ in die Rhizosphäre abzugeben. In einem Lysimeterversuch mit einem kalkreichen Natriumboden (pHs = 7, 4; ECe = 3, 1 dS m -1; SAR = 28, 4; ESP = 27, 6; CaCO3 = 50 g kg -1) wurde die Möglichkeit einer Phytomelioration mit N2 -fixierender Luzerne (Medicago sativa L.) im Vergleich zu einer mit mineralischem N ernährten Luzerne (NH4NO3) untersucht. In weiteren Varianten (Applikation von Gips bzw. Schwefelsäure) wurde die chemische Melioration einer nicht behandelten Kontrolle gegenübergestellt. Beide Ernährungsformen führten zu statistisch ähnlicher Wurzelund Sprossmasse der Luzerne. Nach zweimonatigem Pflanzenwachstum erfolgte alle 24±1 h eine Dränung der Lysimeter durch Zugabe einer Wassermenge von 130% der maximalen Wasserkapazität zum Boden. Hinsichtlich der Effizienz, Na+ über Auswaschung aus dem Boden zu entfernen, zeigte sich folgende Reihenfolge: Schwefelsäure > Gips = N2 -fixierende Luzerne > NH4NO3 -ernährte Luzerne > Kontrolle. Wir führen das bessere Meliorationsergebnis in der Variante der N2 -fixierenden Luzerne auf eine zusätzliche H+ -Quelle in der Rhizosphäre zurück, die zur Lösung von zusätzlichem CaCO3 beitrug. [source] Effect of nitrogen source in the fertilizing solution on nutritional quality of three members of the Portulaca oleracea aggregateJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 12 2010Gabriella Szalai Abstract BACKGROUND:Portulaca oleracea (purslane) is nutritious but, in addition to the essential ,-linolenic acid, vitamin C and tocopherols, it contains undesirable oxalic acid. Knowing the effects of nitrate and ammonium on oxalate accumulation, we tested the agronomic potential of three members of the P. oleracea aggregate under various nitrogen fertilization conditions, by measuring biomass production and accumulation of fatty acids, organic acids and tocopherol in the commercial P. sativa (Pos) and two natural members: P. nitida (Pon) and P. papillato-stellulata (Pop). RESULTS:With nitrate as the sole N source, we measured differences between Pon and Pos in concentrations of the essential ,-3 fatty acid ,-linolenic acid. Pos also gained less dry biomass under these conditions, implying a higher agronomical and nutritional value for Pon. Increasing the fertilizer ammonium concentration and reducing that of nitrate significantly decreased oxalic acid by factors of up to 1.7, 2.6 and 3.4 in Pos, Pop and Pon, respectively, significantly increased concentrations of tocopherol and malic acid, had no effect on fatty acids or ascorbic acid, but reduced biomass. CONCLUSION:In spite of the recumbent growth habit of Pon, the present findings indicate its agronomic potential. Because early flowering and seed production may be the limiting factors in purslane agriculture, growing Pon in nitrate-poor conditions might be agriculturally favorable. Copyright © 2010 Society of Chemical Industry [source] Sequestration of soil nitrogen as tannin,protein complexes may improve the competitive ability of sheep laurel (Kalmia angustifolia) relative to black spruce (Picea mariana)NEW PHYTOLOGIST, Issue 1 2009G. D. Joanisse Summary ,,The role of litter tannins in controlling soil nitrogen (N) cycling may explain the competitive ability of Kalmia relative to black spruce (Picea mariana), although this has not been demonstrated experimentally. ,,Here, the protein-precipitation capacities of purified tannins and leaf extracts from Kalmia and black spruce were compared. The resistance to degradation of tannin,protein precipitates from both species were compared by monitoring carbon (C) and N dynamics in humus amended with protein, purified tannins or protein,tannin precipitates. The purity of the precipitates was verified using solid-state 13C nuclear magnetic resonance (NMR) spectra. The ability of mycorrhizal fungi associated with both species to grow on media amended with tannin,protein complexes as the principal N source was also compared. ,,The protein precipitation capacity of Kalmia tannins was superior to those of black spruce. Humus amended with protein increased both mineral and microbial N, whereas humus amended with tannin,protein precipitates increased dissolved organic N. Mycorrhizal fungi associated with Kalmia showed better growth than those associated with black spruce when N was provided as tannin,protein precipitates. ,,These data suggest that Kalmia litter increases the amount of soil N sequestered as tannin,protein complexes, which may improve the competitive ability of Kalmia relative to black spruce by favouring N uptake by mycorrhizas associated with the former. [source] Nodulated N2 -fixing Casuarina cunninghamiana is the sink for net N transfer from non-N2 -fixing Eucalyptus maculata via an ectomycorrhizal fungus Pisolithus sp. using 15NH4+ or 15NO3, supplied as ammonium nitrateNEW PHYTOLOGIST, Issue 3 2005Xinhua He Summary ,,To determine the effects of nitrogen source on rates of net N transfer between plants connected by a common mycorrhizal network, we measured transfer of N supplied as 15NH414NO3 or 14NH415NO3 in three Casuarina/Eucalyptus treatments interconnected by a Pisolithus sp. The treatments were nonnodulated nonmycorrhizal/nonmycorrhizal; nonnodulated mycorrhizal/mycorrhizal; and nodulated mycorrhizal/mycorrhizal. ,,Mycorrhization was 67% in Eucalyptus and 36% in Casuarina. N2 fixation supplied 38% of the N in Casuarina. Biomass, N and 15N contents were lowest in nonmycorrhizal plants and greatest in plants in the nodulated/mycorrhizal treatment. ,,Nitrogen transfer was enhanced by mycorrhization and by nodulation, and was greater when N was supplied as 15NH4+ than 15NO3,. Nitrogen transfer rates were lowest in the nonmycorrhizal treatment for either 15N source, and greatest in the nodulated, mycorrhizal treatment. Transfer was greater to Casuarina than to Eucalyptus and where ammonium rather than nitrate was the N source. ,,Irrespective of 15N source and of whether Casuarina or Eucalyptus was the N sink, net N transfer was low and was similar in both nonnodulated treatments. However, when Casuarina was the N sink in the nodulated, mycorrhizal treatment, net N transfer was much greater with 15NH4+ than with 15NO3,. High N demand by Casuarina resulted in greater net N transfer from the less N-demanding Eucalyptus. Net transfer of N from a non-N2 -fixing to an N2 -fixing plant may reflect the very high N demand of N2 -fixing species. [source] GaN/Al0.5Ga0.5N quantum dots and quantum dashesPHYSICA STATUS SOLIDI (B) BASIC SOLID STATE PHYSICS, Issue 4 2009Thomas Huault Abstract GaN/Al0.5Ga0.5N nanostructures grown on c -plane sapphire by molecular beam epitaxy using ammonia as N source have been studied. Depending on the ammonia pressure during the two dimensional , three dimensional transition of the GaN layer, the shape of the islands is strongly modified: elongated or isotropic islands are observed, leading to the formation of quantum dashes or quantum dots, respectively. This shape transition is seen as a consequence of a change in surface energy. The change of morphology of the GaN layer is clearly evidenced by photoluminescence measurements, and a large redshift in the emission peak is observed for quantum dashes as compared to quantum dots. An electric field ,3 MV/cm is estimated from the measurements at an excitation power ,20 mW/cm2. Weak photoluminescence quenching between low and room temperature for both QDs and QDashes structures is observed, indicating a strong confinement of carriers into the nanostructures. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Effects of temperature and fertilization on nitrogen cycling and community composition of an urban lawnGLOBAL CHANGE BIOLOGY, Issue 9 2008NEETA S. BIJOOR Abstract We examined the influence of temperature and management practices on the nitrogen (N) cycling of turfgrass, the largest irrigated crop in the United States. We measured nitrous oxide (N2O) fluxes, and plant and soil N content and isotopic composition with a manipulative experiment of temperature and fertilizer application. Infrared lamps were used to increase surface temperature by 3.5±1.3 °C on average and control and heated plots were split into high and low fertilizer treatments. The N2O fluxes increased following fertilizer application and were also directly related to soil moisture. There was a positive effect of warming on N2O fluxes. Soils in the heated plots were enriched in nitrogen isotope ratio (,15N) relative to control plots, consistent with greater gaseous losses of N. For all treatments, C4 plant C/N ratio was negatively correlated with plant ,15N, suggesting that low leaf N was associated with the use of isotopically depleted N sources such as mineralized organic matter. A significant and unexpected result was a large, rapid increase in the proportion of C4 plants in the heated plots relative to control plots, as measured by the carbon isotope ratio (,13C) of total harvested aboveground biomass. The C4 plant biomass was dominated by crabgrass, a common weed in C3 fescue lawns. Our results suggest that an increase in temperature caused by climate change as well as the urban heat island effect may result in increases in N2O emissions from fertilized urban lawns. In addition, warming may exacerbate weed invasions, which may require more intensive management, e.g. herbicide application, to manage species composition. [source] Estimating Background and Threshold Nitrate Concentrations Using Probability GraphsGROUND WATER, Issue 5 2006S.V. Panno Because of the ubiquitous nature of anthropogenic nitrate () in many parts of the world, determining background concentrations of in shallow ground water from natural sources is probably impossible in most environments. Present-day background must now include diffuse sources of such as disruption of soils and oxidation of organic matter, and atmospheric inputs from products of combustion and evaporation of ammonia from fertilizer and livestock waste. Anomalies can be defined as derived from nitrogen (N) inputs to the environment from anthropogenic activities, including synthetic fertilizers, livestock waste, and septic effluent. Cumulative probability graphs were used to identify threshold concentrations separating background and anomalous NO3 -N concentrations and to assist in the determination of sources of N contamination for 232 spring water samples and 200 well water samples from karst aquifers. Thresholds were 0.4, 2.5, and 6.7 mg/L for spring water samples, and 0.1, 2.1, and 17 mg/L for well water samples. The 0.4 and 0.1 mg/L values are assumed to represent thresholds for present-day precipitation. Thresholds at 2.5 and 2.1 mg/L are interpreted to represent present-day background concentrations of NO3 -N. The population of spring water samples with concentrations between 2.5 and 6.7 mg/L represents an amalgam of all sources of in the ground water basins that feed each spring; concentrations >6.7 mg/L were typically samples collected soon after springtime application of synthetic fertilizer. The 17 mg/L threshold (adjusted to 15 mg/L) for well water samples is interpreted as the level above which livestock wastes dominate the N sources. [source] Controls on surface water chemistry in two lake-watersheds in the Adirondack region of New York: differences in nitrogen solute sources and sinksHYDROLOGICAL PROCESSES, Issue 10 2007Mari Ito Abstract The southwestern Adirondack region of New York receives among the highest rates of atmospheric nitrogen (N) deposition in the USA. Atmospheric N deposition to sensitive ecosystems, like the Adirondacks, may increase the acidification of soils through losses of exchangeable nutrient cations, and the acidification of surface waters associated with enhanced mobility of nitrate (NO3,). However, watershed attributes, including surficial terrestrial characteristics, in-lake processing, and geological settings, have been found to complicate the relationships between atmospheric N deposition and N drainage losses. We studied two lake-watersheds in the southwestern Adirondacks, Grass Pond and Constable Pond, which are located in close proximity (,26 km) and receive similarly high N deposition, but have contrasting watershed attributes (e.g. wetland area, geological settings). Since the difference in the influence of N deposition was minimal, we were able to examine both within- and between-watershed influences of land cover, the contribution of glacial till groundwater inputs, and in-lake processes on surface water chemistry with particular emphasis on N solutes and dissolved organic carbon (DOC). Monthly samples at seven inlets and one outlet of each lake were collected from May to October in 1999 and 2000. The concentrations of NO3, were high at the Grass Pond inlets, especially at two inlets, and NO3, was the major N solute at the Grass Pond inlets. The concentrations of likely weathering products (i.e. dissolved Si, Ca2+, Mg2+, Na+) as well as acid neutralizing capacity and pH values, were also particularly high at those two Grass Pond inlets, suggesting a large contribution of groundwater inputs. Dissolved organic N (DON) was the major N solute at the Constable Pond inlets. The higher concentrations of DON and DOC at the Constable Pond inlets were attributed to a large wetland area in the watershed. The DOC/DON ratios were also higher at the Constable Pond inlets, possibly due to a larger proportion of coniferous forest area. Although DON and DOC were strongly related, the stronger relationship of the proportion of wetland area with DOC suggests that additional factors regulate DON. The aggregated representation of watershed physical features (i.e. elevation, watershed area, mean topographic index, hypsometric-analysis index) was not clearly related to the lake N and DOC chemistry. Despite distinctive differences in inlet N chemistry, NO3, and DON concentrations at the outlets of the two lakes were similar. The lower DOC/DON ratios at the lake outlets and at the inlets having upstream ponds suggest the importance of N processing and organic N sources within the lakes. Although an inverse relationship between NO3, and DOC/DON has been suggested to be indicative of a N deposition gradient, the existence of this relationship for sites that receive similar atmospheric N deposition suggest that the relationship between NO3, and the DOC/DON ratio is derived from environmental and physical factors. Our results suggest that, despite similar wet N deposition at the two watershed sites, N solutes entering lakes were strongly affected by hydrology associated with groundwater contribution and the presence of wetlands, whereas N solutes leaving lakes were strongly influenced by in-lake processing. Copyright © 2006 John Wiley & Sons, Ltd. [source] Comparative Effect of Nitrogen Sources on Maize under Saline and Non-saline ConditionsJOURNAL OF AGRONOMY AND CROP SCIENCE, Issue 4 2008M. Irshad Abstract The main objective of this study was to compare the relationship between biomass yield and nutrient uptake in salt-stressed maize (Zea mays L.) following nitrogen (N) nutrition in a greenhouse. Three forms of N were applied, each at the rate of 100 kg ha,1: urea-N, nitrate-N, 1/2 urea-N + 1/2 nitrate-N (mixed-N) and no N application (control). Maize was grown as a test crop for 6 weeks. All N sources greatly stimulated crop growth and nutrient uptake compared with the control. The biomass (shoot and root) of maize was significantly greater in mixed-N treatment than in single sources in saline soil whereas it varied in the order of urea-N > mixed-N > nitrate-N > control in non-saline soil. Under both soil conditions, the concentration of Ca, Mg and Na in shoot was highest in nitrate-N treatments while that of K was highest in the control. Shoot nitrogen concentration was not significantly different among N sources under non-saline treatment, whereas under saline conditions, the concentration varied markedly in the order of nitrate-N > urea-N > mixed-N > control. The mineral concentrations in the shoot increased under salt treated soil when compared with non-saline soil. The ratios of Na/K, Na/Ca and Na/Mg were also higher under salt stress due to higher accumulation of Na ion in the shoot. Among N-fertilizer sources, Na/Ca and Na/Mg ratios were highest in control whereas Na/K ratio was the highest in nitrate-N treatment. The lowest cation ratios were noted in mixed-N-treated plants under both soils. Regression analysis showed that maize biomass was related to N concentration by the following equations: Y = ,4.54 + 0.97N for the non-saline soil and Y = 0.89 + 0.25N for the saline soil. Nitrogen use efficiency for non-saline soil exceeded that of saline soil by 15 %. [source] Effects of aromatic amino acids, phenylacetate and phenylpropionate on fermentation of xylan by the rumen anaerobic fungi, Neocallimastix frontalis and Piromyces communisJOURNAL OF APPLIED MICROBIOLOGY, Issue 4 2007A.Y. Guliye Abstract Aims:, Anaerobic fungi are important members of the fibrolytic community of the rumen. The aim of this study was to study their requirement for aromatic amino acids (AA) and related phenyl acids (phenylpropionic and phenylacetic acids) for optimal xylan fermentation. Methods and Results:,Neocallimastix frontalis RE1 and Piromyces communis P were grown in a defined medium containing oat spelts xylan as the sole energy source, plus one of the following N sources: ammonia; ammonia plus a complete mixture of 20 AA commonly found in protein; ammonia plus complete AA mixture minus aromatic AA; ammonia plus phenyl acids; ammonia plus complete AA mixture without aromatic AA plus phenyl acids. Both species grew in all the media, indicating no absolute requirement for AA. The complete AA mixture increased (P < 0·05) acetate concentration by 18% and 15%, sugar utilization by 33% and 22% and microbial yield by about 22% and 15% in N. frontalis and P. communis, respectively, in comparison with the treatments that had ammonia as the only N source. Neither the supply of aromatic AA or phenol acids, nor their deletion from the complete AA mixture, affected the fermentation rate, products or yield of either species. Conclusions:, AA were not essential for N. frontalis and P. communis, but their growth on xylan was stimulated. The effects could not be explained in terms of aromatic AA alone. Significance and Impact of the Study:, Ruminant diets should contain sufficient protein to sustain optimal fibre digestion by ruminal fungi. Aromatic AA or phenyl acids alone cannot replace the complete AA mixture. [source] RAPID AMMONIUM- AND NITRATE-INDUCED PERTURBATIONS TO CHL a FLUORESCENCE IN NITROGEN-STRESSED DUNALIELLA TERTIOLECTA (CHLOROPHYTA),JOURNAL OF PHYCOLOGY, Issue 2 2003Erica B. Young When NH4+ or NO3, was supplied to NO3, -stressed cells of the microalga Dunaliella tertiolecta Butcher, immediate transient changes in chl a fluorescence were observed over several minutes that were not seen in N-replete cells. These changes were predominantly due to nonphotochemical fluorescence quenching. Fluorescence changes were accompanied by changes in photosynthetic oxygen evolution, indicating interactions between photosynthesis and N assimilation. The magnitude of the fluorescence change showed a Michaelis-Menten relationship with half-saturation concentration of 0.5 ,M for NO3, and 10 ,M for NH4+. Changes in fluorescence responses were characterized in D. tertiolecta both over 5 days of N starvation and in cells cultured at a range of NO3, -limited growth rates. Variation in responses was more marked in starved than in limited cells. During N starvation, the timing and onset of the fluorescence responses were different for NO3, versus NH4+ and were correlated with changes in maximum N uptake rate during N starvation. In severely N-starved cells, the major fluorescence response to NO3, disappeared, whereas the response to NH4+ persisted. N-starved cells previously grown with NH4+ alone showed fluorescence responses with NH4+ but not NO3, additions. The distinct responses to NO3, and NH4+ may be due to the differences between regulation of the uptake mechanisms for the two N sources during N starvation. This method offers potential for assessing the importance of NO3, or NH4+ as an N source to phytoplankton populations and as a diagnostic tool for N limitation. [source] EXPLORING PORPHYRA SPECIES FOR USE AS NITROGEN SCRUBBERS IN INTEGRATED AQUACULTUREJOURNAL OF PHYCOLOGY, Issue 2001Article first published online: 24 SEP 200 Carmona, R.1, Kraemer G. P.2, Zertuche, J. A.3, Chanes, L.4, Chopin, T.5, Neefus C.4,6 & Yarish, C.1 1Dept. of Ecol. and Evol. Biol., University of Connecticut, One University Place, Stamford, CT 06901, USA; 2Department of Environmental Sciences, State University of New York, Purchase, NY 10577 USA; 3IIO, Universidad Autonoma de Baja California. Ensenada,B.C., Mexico; 4DGETI-CBTis41, Mexico; 5CCSA, Dept. of Biol., University of New Brunswick, Saint John, N.B., E2L 4L5, Canada; 6Department of Plant Biology, University of New Hampshire, Durham, NH 03824, USA Finfish mariculture along the Northeast US coast continues to develop into a strong industry. At a regional level, mariculture can be a significant contributor to nutrient loading in coastal waters. Since macroalgae are able to concentrate nutrients and grow at high rates, they can be an useful tool for alleviating this problem. In addition, seaweed mariculture is by itself a multi-billion dollar industry, with the red alga Porphyra (nori) valued at over $US 1.8 billion. Local species and strains of Porphyra from the Northeast U.S.A. are being studied to determine their capacity as nutrient scrubbers under different nutrient and temperature conditions. P. purpurea was grown under two N sources (NO3- vs. NH4+). The fastest growth (up to 13% d-1) and greatest N content (ca. 7% DW) were measured in plants grown at 300 µM NH4+. Short-term NH4+ uptake by P. purpurea (strains from Maine and Long Island Sound) and by P. amplissima was not saturated at 150 µM, the highest concentration tested. The P. purpurea isolate from Maine took up NH4+ faster than did the Long Island Sound isolate. NH4+ uptake by P. amplissima was faster than uptake by either P. purpurea strain. The high growth rates obtained and the ability for N uptake and tissue accumulation make these species suitable for using as a biological nutrient removal system. [source] Effect of the nitrification inhibitor nitrapyrin on the fate of nitrogen applied to a soil incubated under laboratory conditionsJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 4 2003M. Kaleem Abbasi Abstract The aim of this study was to examine the effect of the nitrification inhibitor nitrapyrin on the fate and recovery of fertilizer nitrogen (N) and on N mineralization from soil organic sources. Intact soil cores were collected from a grassland field. Diammonium phosphate (DAP) and urea were applied as N sources. Cores were equilibrated at ,5 kPa matric potential and incubated at 20,°C for 42 to 56 days. Changes in NH4+ -N, accumulation of NO3, -N, apparent recovery of applied N, and emission of N2O (acetylene was used to block N2O reductase) were examined during the study. A significant increase in NH4+ -N released through mineralization was recorded when nitrapyrin was added to the control soil without N fertilizer application. In the soils to which N was added either as urea or DAP, 50,90,% of the applied N disappeared from the NH4+ -N pool. Some of this N (8,16,%) accumulated as NO3, -N, while a small proportion of N (1,%) escaped as N2O. Addition of nitrapyrin resulted in a decrease and delay of NH4+ -N disappearance, accumulation of much lower soil NO3, -N contents, a substantial reduction in N2O emissions, and a 30,40,% increase in the apparent recovery of added N. The study indicates that N recovery can be increased by using the nitrification inhibitor nitrapyrin in grassland soils at moisture condition close to field capacity. No translation. [source] Natural 15N abundance of epiphytes depends on the position within the forest canopy: source signals and isotope fractionationPLANT CELL & ENVIRONMENT, Issue 4 2002R. Wania Abstract The natural 15N abundance (,15N) of epiphytes and its N sources were studied in the canopy of a lowland rainforest in Costa Rica. Vascular and non-vascular epiphytes and canopy soils were collected from four canopy zones and analysed for N contents and ,15N signals. In addition, the N concentrations and ,15N signatures of bulk precipitation, throughfall and stemflow were measured during the wet and the dry season. The ,15N values of epiphyte leaves decreased significantly from the lower zones (means of ,3·9 and ,4·3,) to the upper zones (means of ,5·4 and ,6·1,) of the canopy. In contrast, ,15N signatures of canopy soils (average ,0·3,) differed little between the zones. Bulk deposition was enriched in 15N (+4·3,) compared to all other potential N sources and was higher than throughfall and stemflow (+0·5 to ,1·3,). ,15N values of atmospheric deposition were inversely related to those of the epiphyte leaves, whereas N isotopic composition of canopy soils did not vary significantly. Consequently, it is concluded that the variations in foliar N isotope composition of epiphytes were not simply caused by utilization of isotopically different N sources, but by different 15N discrimination during N acquisition. [source] Modelling of reserve carbohydrate dynamics, regrowth and nodulation in a N2 -fixing tree managed by periodic pruningsPLANT CELL & ENVIRONMENT, Issue 10 2000F. Berninger ABSTRACT We used a modified transport resistance approach to model legume tree growth, nodulation and dynamics of reserve carbohydrates after pruning. The model distributes growth between roots and shoots applying the transport resistance approach. Within shoots, growth is divided into leaves, branches and stems applying the pipe model theory. The model also accounts for the metabolic differences of principal N sources, nitrate, ammonium and atmospheric dinitrogen, in a mechanistic way. We compared the simulation results with measured biomass dynamics of Gliricidia sepium (Jacq.) Walp. (Papilionaceae: Robinieae) under humid and subhumid tropical conditions. Comparison showed that the biomass production predicted by the model is close to measured values. Total N2 fixation is also similar to measured values. Qualitatively the model increases the proportion of N2 fixation if roots acquire less mineral N. In the present study, the general form of the model is discussed and compared with similar models. The results encourage the use of this approach for studying biomass dynamics of legume trees under the scheme of periodic prunings. Also, it shows that process-based models have potential in the simulation of trees disturbed by prunings, herbivory or similar factors. [source] Characterization of agrobacteria from weeping fig (Ficus benjamina)PLANT PATHOLOGY, Issue 5 2001A. Zoina Ficus benjamina plants, galled both at epi- and hypogeous parts, were observed in Italy and in The Netherlands, and these were the first records of the appearance of weeping fig crown gall in Europe. A total of 241 Agrobacterium isolates was obtained from 41 tumours and studied for their morphological, physiological and phytopathological characters. Two main groups of agrobacteria were distinguished by their colony morphology and through classical biovarietal tests that allowed allocation of 86 isolates into biovar 1 and 155 into an intermediate biovar rather different from any of the three biovars defined for agrobacteria. Most of the isolates were unable to utilize mannopine, nopaline or octopine as C and N sources; only 62 strains utilized nopaline. However, when nonopine-utilizing strains were inoculated into F. benjamina, only nopaline was detected in the developing tumours. BIOLOG ML 1Ô system analysis applied to 50 representative strains allowed identification of the biovar 1 isolates as Agrobacterium tumefaciens and most of the intermediate biovar isolates as the newly proposed species Agrobacterium fici. Analysis of sensitivity to a set of 14 antibiotics confirmed the allocation of the 50 strains into two well defined main clusters matching the BIOLOG identification. Out of 141 tumorigenic isolates, 66 were sensitive in vitro to agrocine 84, but four of these strains showed scarce or no sensitivity to the antagonist A. radiobacter K84 when tested in fig plants. The two types of agrobacteria could usually be isolated from the same tumours. Tumorigenic strains were able to induce tumours in six herbaceous plant species, in eight to 10 out of 12 woody plants and in six to eight out of nine Ficus species, indicating a wide host-range Ti plasmid. Agrobacteria were able to survive and move in the vascular system of galled ficus plants and to induce tumour growth in stem-cutting propagated plants. Moreover, agrobacteria were detected in many healthy F. benjamina plants as part of the endophytic microflora. These findings suggest potential for spread of the disease through latently infected plant propagation material produced as cuttings or by tissue culture. [source] The comparative study digestion and metabolism of nitrogen and purine derivatives in male, Thai, Swamp buffalo and Thai, Brahman cattleANIMAL SCIENCE JOURNAL, Issue 2 2009Thongsuk JETANA ABSTRACT Studies on in vivo digestion, rates of passages, metabolism of nitrogen, urinary purine derivative excretion and blood metabolites were carried out in Thai Brahman cattle and Thai swamp buffaloes (16 months old). The animals were fed mixed diets based on pineapple (Ananas comusus) waste silage containing urea-N (NPN) and true protein from a concentrate (TP). The Brahman cattle (310 ± 15 kg) were heavier than the swamp buffaloes (195 ± 9.4 kg) and had higher dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) intakes when compared on the basis of their metabolic body weight (BW0.75), but these intakes did not differ significantly when the diets of each animal species were compared. The total tract, apparent digestibilities of dry matter (DM) and organic matter (OM) were not significantly different between the animal species when comparing the two types of diets. The NDF digestibility was significantly (P < 0.01) decreased in both animal species when fed the TP diet, but was significantly (P < 0.01) greater in cattle than in swamp buffaloes. The passage rate digesta k1 (P < 0.01) and the passage rate digesta k2 (through the caecum and proximal colon) (P < 0.03) were significantly slower, and the total mean retention time (TMRT) (P < 0.01) was significantly longer in swamp buffaloes when compared to Brahman cattle, but the transit time (TT) showed no difference (P = 0.07) between the animal species or the diets. The N intakes were not different in both animal species and diets, but urine-N was greater (P < 0.05) in Brahman cattle than that in swamp buffaloes. Urine N and digestibility of N were significantly (P < 0.04) higher in animals fed the NPN diet than those fed the TP diet. Urinary purine derivatives (PD) and the creatinine (Cr) excretion of swamp buffaloes were significantly (P < 0.01) lower than those in Brahman cattle. Plasma urea-N (BUN) concentration was significantly (P < 0.01) higher in swamp buffaloes than that in Brahman cattle, but plasma glucose and insulin concentrations were significantly (P < 0.01) higher in Brahman cattle than in swamp buffaloes. The concentrations of non-esterified fatty acids (NEFA) were not significantly (P > 0.05) different in animals fed different diets. The present study demonstrated that Brahman cattle were better in fiber digestibility than swamp buffaloes at utilizing pineapple waste silage with both N sources. [source] Effects of amino nitrogen on fermentation parameters by mixed ruminal microbes when energy or nitrogen is limitedANIMAL SCIENCE JOURNAL, Issue 2 2007Hiroshi KAJIKAWA ABSTRACT Ruminal microbes harvested from a ruminally fistulated cow were incubated in simple batch and semicontinuous cultures with NH3 -N or amino-N on nitrogen- or energy-excess diets in quantity (HN and LN diets, respectively, consisting of timothy hay plus soybean meal, or corn grain), based on evaluation with the National Research Council and Cornell Net Carbohydrate and Protein System models. In a batch culture experiment, supplementation with amino-N promoted digestion and fermentation in the course of incubation (4,24 h) on both diets, but these effects mostly disappeared when the diets were sufficiently digested (at 48 h). In a semicontinuous culture experiment using Rusitec, no effect of amino-N was exhibited after sufficient fermentation and digestion, but significant promotion of digestion was shown in the course of incubation on the HN diet, while no such effect was detected on the LN diet. The microbial yield for 24 h did not show a significant difference between the N sources of either of the two diets. These results suggest that the stimulatory effects of amino-N are diminished when the diets are sufficiently digested after a long retention and incubation, and also that the effectiveness of amino-N does not require a quantitatively energy-excess status. [source] | |||||||||||||||||||