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Selected AbstractsSusceptibility of a Northern Hardwood Forest to Exotic Earthworm InvasionCONSERVATION BIOLOGY, Issue 4 2005MICHAEL J. GUNDALE Acer saccharum; Bosque Nacional Ottawa; lombrices invasoras; Sylvania Wilderness Area; uso del suelo Abstract:,Numerous exotic earthworm species are colonizing northern hardwood forests of North America, where no native earthworms exist. Upon invasion, earthworms have been shown to alter the surface soil environment and plant populations and communities. We sought to identify land-use factors in the Ottawa National Forest (ONF), Michigan (U.S.A.), that contribute to earthworm invasion in forest dominated by sugar maple (Acer saccharum Marsh.) so that the susceptibility to additional colonization could be evaluated. We sampled earthworm communities in Sylvania Wilderness Area, a unique old-growth hardwood forest, and nonwilderness sites influenced by recreational fishing, recent timber harvesting, or roads. All the nonwilderness sites contained one to five species of exotic earthworms. In contrast, only 50% of wilderness sites contained exotic earthworms, all of a single species. Nonwilderness sites also had thinner litter and duff layers, higher soil C and N content, and higher nitrogen mineralization potentials than Sylvania sites. Two central differences between Sylvania and nonwilderness sites were that all nonwilderness sites were in close contact with roads and had a history of timber harvest, whereas these factors were not present in Sylvania Wilderness Area. Using average rates of colonization, we constructed two geographic information system models to estimate the percentage of sugar maple on the ONF falling within a theoretical 100-year invasion distance of roads and of second-growth sugar maple as relative indices of susceptibility to invasion. Both models indicated high susceptibility to invasion, with 91.7% and 98.9% of sugar maple habitat falling within a theoretical 100-year invasion distance of roads or historical harvests, respectively. Resumen:,Numerosas especies de lombrices exóticas están colonizando los bosques boreales, en los que previamente no existían lombrices terrestres nativas. Por encima de la invasión, se ha demostrado que las lombrices alteran el ambiente superficial del suelo, así como a las comunidades y poblaciones de plantas. Tratamos de identificar factores de uso del suelo en el Bosque Nacional Ottawa (BNO), Michigan, E. U. A., que contribuyen a la invasión de lombrices en bosques dominados por arces (Acer saccharum Marsh.), para poder evaluar la susceptibilidad a futuras invasiones. Muestreamos comunidades de lombrices en Sylvania Wilderness Area, un bosque maduro único, y en sitios no silvestres influenciados por pesca deportiva, cosecha reciente de madera o caminos. Todas las áreas no silvestres contenían 1 - 5 especies de lombrices exóticas. En contraste, solo 50% de los sitios silvestres contenían lombrices exóticas, todas de una sola especie. Los sitios no silvestres también tenían capas de hojarasca y de mantillo más delgadas, mayor contenido de C y N del suelo y mayor potencial de mineralización del nitrógeno que los sitios en Sylvania. Dos diferencias centrales entre Sylvania y los sitios no silvestres fueron que estos estaban en contacto cercano con caminos y tenían una historia de cosecha de madera, mientras que estos factores no estuvieron presentes en Sylvania Wilderness Area. Utilizando tasas promedio de colonización, construimos dos modelos de sistemas de información geográfica para estimar el porcentaje de arces en el BNO que queda a una distancia teórica de invasión en 100 años; con caminos y arce de crecimiento secundario como índices relativos de susceptibilidad a la invasión. Ambos modelos indicaron alta susceptibilidad a la invasión, con 91.7% y 98.9% del hábitat de arce dentro de la distancia teórica de invasión en 100 años o con cosechas históricas, respectivamente. [source] Phosphorus-limited growth dynamics in two Baltic Sea cyanobacteria, Nodularia sp. and Aphanizomenon sp.FEMS MICROBIOLOGY ECOLOGY, Issue 3 2006Jenny Degerholm Abstract Rates of carbon (C) specific growth and nitrogen (N2) fixation were monitored in cultures of Baltic Sea Nodularia and Aphanizomenon exposed to gradual limitation by inorganic phosphorus (P). Both cyanobacteria responded by decreased cellular P content followed by lowered rates of growth and N2 fixation. C-specific growth and cellular N content changed faster in Aphanizomenon both when inorganic P was lowered as well as during reintroduction of P. Aphanizomenon also showed a more rapid increase in N-specific N2 fixation associated with increased C-specific growth. When ambient concentrations of inorganic P declined, both cyanobacteria displayed higher rates of alkaline phosphatase (APase) activity. Lower substrate half-saturation constants (KM) and higher Vmax : KM ratio of the APase enzyme associated with Nodularia suggest a higher affinity for dissolved organic P (DOP) substrate than Aphanizomenon. Aphanizomenon, which appears more sensitive to changes in ambient dissolved inorganic P, may be adapted to environments with elevated concentrations of P or repeated intrusions of nutrient-rich water. Nodularia on the other hand, with its higher tolerance to increased P starvation may have an ecological advantage in stratified surface waters of the Baltic Sea during periods of low P availability. [source] Effects of increased temperature and nutrient enrichment on the stoichiometry of primary producers and consumers in temperate shallow lakesFRESHWATER BIOLOGY, Issue 7 2008M. VENTURA Summary 1. We studied the effects of increased water temperatures (0,4.5 °C) and nutrient enrichment on the stoichiometric composition of different primary producers (macrophytes, epiphytes, seston and sediment biofilm) and invertebrate consumers in 24 mesocosm ecosystems created to mimic shallow pond environments. The nutrient ratios of primary producers were used as indicative of relative nitrogen (N) or phosphorus (P) limitation. We further used carbon stable isotopic composition (,13C) of the different primary producers to elucidate differences in the degree of CO2 limitation. 2. Epiphytes were the only primary producer with significantly higher ,13C in the enriched mesocosms. No temperature effects were observed in ,13C composition of any primary producer. Independently of the treatment effects, the four primary producers had different ,13C signatures indicative of differences in CO2 limitation. Seston had signatures indicating negligible or low CO2 limitation, followed by epiphytes and sediment biofilm, with moderate CO2 limitation, while macrophytes showed the strongest CO2 limitation. CO2 together with biomass of epiphytes were the key variables explaining between 50 and 70% of the variability in ,13C of the different primary producers, suggesting that epiphytes play an important role in carbon flow of temperate shallow lakes. 3. The ratio of carbon to chlorophyll a decreased with increasing temperature and enrichment in both epiphytes and seston. The effects of temperature were mainly attributed to changes in algal Chl a content, while the decrease with enrichment was probably a result of a higher proportion of algae in the seston and epiphytes. 4. Macrophytes, epiphytes and seston decreased their C : N with enrichment, probably as an adaptation to the different N availability levels. The C : N of epiphytes and Elodea canadensis decreased with increasing temperature in the control mesocosms. Sediment biofilm was the only primary producer with lower C : P and N : P with enrichment, probably as a result of higher P accumulation in the sediment. 5. Independently of nutrient level and increased temperature effects the four primary producers had significantly different stoichiometric compositions. Macrophytes had higher C : N and C : P and, together with epiphytes, also the highest N : P. Seston had no N or P limitation, while macrophytes and epiphytes may have been P limited in a few mesocosms. Sediment biofilm indicated strong N deficiency. 6. Consumers had strongly homeostatic stoichiometric compositions in comparison to primary producers, with weak or no significant treatment effects in any of the groups (insects, leeches, molluscs and crustaceans). Among consumers, predators had significantly higher N content and lower C : N than grazers. [source] Food quality for Daphnia in humic and clear water lakesFRESHWATER BIOLOGY, Issue 2 2007KELLY GUTSEIT Summary 1. Growth and reproduction of Daphnia fed lake seston were measured in two categories of meso- to eutrophic lakes differing with respect to terrestrial organic matter influence (humic and clear water lakes). The content of highly unsaturated fatty acids (HUFA), P and N, as well as the taxonomical composition of seston were analysed. 2. Seston HUFA and C : P ratios were similar between lake categories, whereas C : N ratios were lower in the clear water lakes in both spring and summer. Despite the similarity in HUFA and P content of seston, Daphnia growth rate, clutch size and the proportion of gravid females were, respectively, about 1.5, 3 and 6 times higher in the clear water lakes. 3. Differences in growth and reproduction were related to a combination of higher N content and good fatty acid quality of the seston in the clear water lakes. Relatively high biomass of edible algae, such as Rhodomonas sp. and Cryptomonas sp., in the clear water lakes, and differences in water pH likely contributed to the observed differences in Daphnia growth and reproduction between lake categories. Additionally, it is possible that Daphnia was energy limited in the humic lakes despite high particulate organic carbon (POC) concentrations, as the contribution of non-algal and detrital C to the POC pool was high. 4. Our results suggest that dietary HUFA content has the potential to improve herbivore growth and reproduction if N and P are not limiting. N merits more attention in studies of zooplankton nutrition. [source] Taxonomic and regional patterns in benthic macroinvertebrate elemental composition in streamsFRESHWATER BIOLOGY, Issue 11 2005MICHELLE A. EVANS-WHITE Summary 1. Ecological stoichiometry has been used to better understand dynamics in consumer growth and the role of consumer-recycled nutrients because it focuses on more than one element. Most research has focused on pelagic rather than benthic consumers. Variation in elemental composition among benthic consumer taxa would suggest that taxa differ in their susceptibility to nutrient limitation or in their role in recycling nutrients. 2. We collected benthic macroinvertebrates from streams in two regions (Indiana,Michigan and Wisconsin, U.S.A.) to examine taxonomic and regional variation in benthic macroinvertebrate body carbon (C), nitrogen (N), and phosphorus (P) concentrations and ratios. 3. Elemental composition varied little within taxa common to both regions. In contrast, elemental composition differed greatly among taxa and appeared to be related to phylogeny. The elemental composition of macroinvertebrates clustered into three distinct groups: insects, mollusks, and crustaceans. To a lesser extent, insects and mollusks also differed in elemental composition among genera. 4. Functional feeding groups (FFGs) differed in elemental composition, with predators having a higher N content than other groups. Substantial elemental imbalances between C and N were found between most primary consumers and their likely food sources, and the magnitude of the imbalance depended in part on the FFG. 5. Our results support an assumption of most ecological stoichiometry models that, within a species, the elemental composition of aquatic invertebrates is relatively constant. Variation in elemental composition among taxa at various higher taxonomic levels suggests that susceptibility of stream invertebrates to nutrient limitation and their role in nutrient cycling will strongly depend on phylogeny. [source] CO2 and nitrogen, but not population density, alter the size and C/N ratio of Phytolacca americana seedsFUNCTIONAL ECOLOGY, Issue 3 2005J.-S. HE Summary 1Plants can provision seeds by optimizing seed size, number and nutrient content to maximize parental fitness. According to the McGinley,Charnov hypothesis, seed size should be determined by the ratio of carbon to nitrogen (C/N) available to the plant, with larger seed size correlating with larger C/N ratios and smaller absolute N content. 2This hypothesis was tested by establishing monocultures of Phytolacca americana L. (Phytolaccaceae) at three population densities under ambient and elevated CO2 environments, with two availabilities of soil N. 3Elevated CO2 reduced both seed size and N concentration while increasing the C/N ratio; high soil N availability produced the opposite result for N concentration and C/N ratio. Higher planting densities reduced plant biomass, but did not alter seed size. 4In accordance with the McGinley,Charnov hypothesis, larger seeds had both larger C/N ratios and smaller N content. However, the increase in C/N ratio caused by elevated CO2 corresponded with smaller seeds overall: elevated CO2 reduced seed size, although the seed size,C/N relationship remained positive. 5These results suggest an alternative mechanism to explain variation in seed size, and suggest that future climate conditions may alter seed quality and plant reproductive behaviour. [source] Effects of temperature and fertilization on nitrogen cycling and community composition of an urban lawnGLOBAL CHANGE BIOLOGY, Issue 9 2008NEETA S. BIJOOR Abstract We examined the influence of temperature and management practices on the nitrogen (N) cycling of turfgrass, the largest irrigated crop in the United States. We measured nitrous oxide (N2O) fluxes, and plant and soil N content and isotopic composition with a manipulative experiment of temperature and fertilizer application. Infrared lamps were used to increase surface temperature by 3.5±1.3 °C on average and control and heated plots were split into high and low fertilizer treatments. The N2O fluxes increased following fertilizer application and were also directly related to soil moisture. There was a positive effect of warming on N2O fluxes. Soils in the heated plots were enriched in nitrogen isotope ratio (,15N) relative to control plots, consistent with greater gaseous losses of N. For all treatments, C4 plant C/N ratio was negatively correlated with plant ,15N, suggesting that low leaf N was associated with the use of isotopically depleted N sources such as mineralized organic matter. A significant and unexpected result was a large, rapid increase in the proportion of C4 plants in the heated plots relative to control plots, as measured by the carbon isotope ratio (,13C) of total harvested aboveground biomass. The C4 plant biomass was dominated by crabgrass, a common weed in C3 fescue lawns. Our results suggest that an increase in temperature caused by climate change as well as the urban heat island effect may result in increases in N2O emissions from fertilized urban lawns. In addition, warming may exacerbate weed invasions, which may require more intensive management, e.g. herbicide application, to manage species composition. [source] Interactive effects of elevated CO2 and soil fertility on isoprene emissions from Quercus roburGLOBAL CHANGE BIOLOGY, Issue 11 2004Malcolm Possell Abstract The effects of global change on the emission rates of isoprene from plants are not clear. A factor that can influence the response of isoprene emission to elevated CO2 concentrations is the availability of nutrients. Isoprene emission rate under standard conditions (leaf temperature: 30°C, photosynthetically active radiation (PAR): 1000 ,mol photons m,2 s,1), photosynthesis, photosynthetic capacity, and leaf nitrogen (N) content were measured in Quercus robur grown in well-ventilated greenhouses at ambient and elevated CO2 (ambient plus 300 ppm) and two different soil fertilities. The results show that elevated CO2 enhanced photosynthesis but leaf respiration rates were not affected by either the CO2 or nutrient treatments. Isoprene emission rates and photosynthetic capacity were found to decrease with elevated CO2, but an increase in nutrient availability had the converse effect. Leaf N content was significantly greater with increased nutrient availability, but unaffected by CO2. Isoprene emission rates measured under these conditions were strongly correlated with photosynthetic capacity across the range of different treatments. This suggests that the effects of CO2 and nutrient levels on allocation of carbon to isoprene production and emission under near-saturating light largely depend on the effects on photosynthetic electron transport capacity. [source] Assessment of the nitrogen status of grasslandGRASS & FORAGE SCIENCE, Issue 2 2004A. Farruggia Abstract Two types of diagnostics are used for N management in grasslands: diagnostics based on N concentration of shoots and diagnostics based on soil mineral N. The Nitrogen Nutrition Index (NNI) is an example of the first type. However, its evaluation requires the determination of shoot dry weight per unit area and, thus, constitutes a practical limit to its utilization in the context of farm studies. In order to simplify its evaluation, a method based on the N concentration of the upper sward layer (Nup) has been proposed. The objectives of this study were to test the relationship between NNI and Nup in the context of permanent grassland and to examine the relationship between Nup and soil mineral status. The study was conducted as two experiments, one on small cut-plots receiving contrasting rates of mineral N fertilization, and a second on plots of an existing field-scale lysimeter experiment. In each plot and at several dates, shoot biomass within quadrats was measured, N concentration was determined on the upper leaves and on the entire shoots, and mineral nitrogen of the soil below the vegetation sampled was determined. N concentration of the upper lamina layer of the canopy was linearly related to the NNI determined on the entire shoots. Therefore, determining N concentration in leaves at the top of canopy appears to be an alternative means to evaluate NNI without having to measure shoot biomass. The absence of an overall significant correlation between soil mineral N content and sward N index, observed over the two studies, indicates that each of these two indicators has to be considered specifically in relation to the objective of the diagnostic procedure. As sward N index may vary independently of soil mineral N content, the sward N indicator does not appear to be a suitable indicator for diagnosis of environmental risks related to nitrate leaching. However, soil mineral N content does not allow the prediction of sward N status and thus is not a suitable indicator of sward growth rate. Although soil mineral N content is an important environmental indicator for nitrate-leaching risks during potential drainage periods, it has a limited diagnosis value with respect to the herbage production function of grasslands. [source] Nitrogen balance and seasonal fluctuations in soil nitrogen contents in a corn (Zea mays L)-rye (Secale cereale L) rotation fieldGRASSLAND SCIENCE, Issue 1 2005Yuzo Kurokawa Abstract In a corn (Zea mays L)-rye (Secale cereale L) rotation field, the N output (plant uptake) and N input (crop residue and fertilizer applied) were measured for two years in order to evaluate the N balance in a forage production field. The soil was Low-humic Andosol (mesic Typic Hapludand). The disappearance of crop residues on both a dry matter and N basis, and the seasonal fluctuations of total, inorganic, and available soil N content in the field were investigated. The interaction between the nitrogen balance and the soil N contents are discussed. The total plant N uptake of the corn and rye exceeded the sum of the N input of the fertilizer applied and the N from crop residues, so the N balance of the corn-rye rotation had a negative value (two year average: ,8.4 gN m,2). After the gradual disappearance of crop residue on the dry matter basis, rapid disappearance on the N basis was observed. At the same time, the C/N ratio decreased to less than 20. The soil available (38.4,55.3 mg kg,1) and inorganic (21.5,45.2 mg kg,1) N had their lowest values in spring. After they increased in early summer, they decreased in late summer and increased in autumn. The total N (0.5,0.7%) gradually decreased during the experimental period. The results indicated that the soil-available N and inorganic N in the present study were highly dependent on the fertilizer-applied N. The relation among management practices, N input and soil available N contents are discussed. It is suggested that the negative N balance is one of the causes for a decrease in soil total N. [source] First-year post-fire erosion rates in Bitterroot National Forest, Montana,HYDROLOGICAL PROCESSES, Issue 8 2007Kevin M. Spigel Abstract Accelerated runoff and erosion commonly occur following forest fires due to combustion of protective forest floor material, which results in bare soil being exposed to overland flow and raindrop impact, as well as water repellent soil conditions. After the 2000 Valley Complex Fires in the Bitterroot National Forest of west-central Montana, four sets of six hillslope plots were established to measure first-year post-wildfire erosion rates on steep slopes (greater than 50%) that had burned with high severity. Silt fences were installed at the base of each plot to trap eroded sediment from a contributing area of 100 m2. Rain gauges were installed to correlate rain event characteristics to the event sediment yield. After each sediment-producing rain event, the collected sediment was removed from the silt fence and weighed on site, and a sub-sample taken to determine dry weight, particle size distribution, organic matter content, and nutrient content of the eroded material. Rainfall intensity was the only significant factor in determining post-fire erosion rates from individual storm events. Short duration, high intensity thunderstorms with a maximum 10-min rainfall intensity of 75 mm h,1 caused the highest erosion rates (greater than 20 t ha,1). Long duration, low intensity rains produced little erosion (less than 0·01 t ha,1). Total C and N in the collected sediment varied directly with the organic matter; because the collected sediment was mostly mineral soil, the C and N content was small. Minimal amounts of Mg, Ca, and K were detected in the eroded sediments. The mean annual erosion rate predicted by Disturbed WEPP (Water Erosion Prediction Project) was 15% less than the mean annual erosion rate measured, which is within the accuracy range of the model. Published in 2007 by John Wiley & Sons, Ltd. [source] The effect of recycle ratio on nitrogen removal in the combined pre-denitrification/nitrification biofilter systemJOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY, Issue 6 2001Ren-Jie Chiou Abstract Previous research has shown that nitrogen in municipal wastewater could be eliminated by a biofilter system. This study investigates the effect of the recycle ratio on total nitrogen removal and the nitrogen component in the effluent. In this study, a system combining pre-denitrification/nitrification biofilters was set up. The experiments which varied the recycle ratio of the nitrifying solution to the influent were carried out in order to determine the amount of the total nitrogen removal and the form of nitrogen in the effluent. These experimental results show that total nitrogen removal might be dependent on nitrification but not on denitrification. Although the operation at a low recycle ratio would result in a lower value of total nitrogen removal, the NH3 -N content of the effluent would be lower and the flow condition would be more stable. In comparison, operation at a higher recycle ratio would result in a lower NO3, -N content in the effluent, while the NH3 -N content in the effluent would be higher. At a recycle ratio of 2.5, the total nitrogen removal is at its maximum. The relationship between specific nitrification rate and recycle ratio is also discussed. © 2001 Society of Chemical Industry [source] EXPLORING PORPHYRA SPECIES FOR USE AS NITROGEN SCRUBBERS IN INTEGRATED AQUACULTUREJOURNAL OF PHYCOLOGY, Issue 2001Article first published online: 24 SEP 200 Carmona, R.1, Kraemer G. P.2, Zertuche, J. A.3, Chanes, L.4, Chopin, T.5, Neefus C.4,6 & Yarish, C.1 1Dept. of Ecol. and Evol. Biol., University of Connecticut, One University Place, Stamford, CT 06901, USA; 2Department of Environmental Sciences, State University of New York, Purchase, NY 10577 USA; 3IIO, Universidad Autonoma de Baja California. Ensenada,B.C., Mexico; 4DGETI-CBTis41, Mexico; 5CCSA, Dept. of Biol., University of New Brunswick, Saint John, N.B., E2L 4L5, Canada; 6Department of Plant Biology, University of New Hampshire, Durham, NH 03824, USA Finfish mariculture along the Northeast US coast continues to develop into a strong industry. At a regional level, mariculture can be a significant contributor to nutrient loading in coastal waters. Since macroalgae are able to concentrate nutrients and grow at high rates, they can be an useful tool for alleviating this problem. In addition, seaweed mariculture is by itself a multi-billion dollar industry, with the red alga Porphyra (nori) valued at over $US 1.8 billion. Local species and strains of Porphyra from the Northeast U.S.A. are being studied to determine their capacity as nutrient scrubbers under different nutrient and temperature conditions. P. purpurea was grown under two N sources (NO3- vs. NH4+). The fastest growth (up to 13% d-1) and greatest N content (ca. 7% DW) were measured in plants grown at 300 µM NH4+. Short-term NH4+ uptake by P. purpurea (strains from Maine and Long Island Sound) and by P. amplissima was not saturated at 150 µM, the highest concentration tested. The P. purpurea isolate from Maine took up NH4+ faster than did the Long Island Sound isolate. NH4+ uptake by P. amplissima was faster than uptake by either P. purpurea strain. The high growth rates obtained and the ability for N uptake and tissue accumulation make these species suitable for using as a biological nutrient removal system. [source] Labile soil organic carbon, soil fertility, and crop productivity as influenced by manure and mineral fertilizers in the tropicsJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 5 2010Siba Prasad Datta Abstract In recent years, organic agriculture has been receiving greater attention because of the various problems like deterioration in soil health and environmental quality under conventional chemical-intensive agriculture. However, little information is available on the comparative study related to the impact of use of mineral fertilizers and organic manures on the soil quality and productivity. A long-term field experiment was initiated in 2001 to monitor some of the important soil-quality parameters and productivity under soybean,wheat crop rotation. The treatments consisted of 0, 30, and 45,kg N ha,1 for soybean and of 0, 120, and 180,kg N ha,1 for wheat. The entire amount of N was supplied to both the crops through urea and farmyard manure (FYM) alone or in combination at 1:1 ratio. Results indicated that Walkley-and-Black C (WBC; chromic acid,oxidizable) exhibited a marginal increase under only organic treatments as compared to control treatment (without fertilizers and manure) after completion of five cropping cycles. In case of labile-C (KMnO4 -oxidizable) content in soil, relatively larger positive changes were recorded under organic, mixed inputs (integrated) and mineral fertilizers as compared to WBC. Maximum improvement in the values of C-management index (CMI), a measure of soil quality was recorded under organic (348,362), followed by mixed inputs (268,322) and mineral fertilizers (198,199) as compared to the control treatment after completion of five cropping cycles. Similarly there was a substantial increase in KCl-extractable N; in Olsen-P; as well as in DTPA-extractable Zn, Fe, and Mn under organic treatments. Although labile soil C positively contributed to the available N, P, K, Zn, Fe, and Mn contents in soil, it did not show any relationship with the grain yield of wheat. After completion of the sixth cropping cycle, organic treatments produced 23% and 39% lower grain yield of wheat as compared to that under urea-treated plots. Relatively higher amount of mineral N in soil at critical growth stages and elevated N content in plant under mineral-fertilizer treatments compared to FYM treatments were responsible for higher yield of wheat under mineral fertilizers. [source] Establishment of Retama sphaerocarpa L. seedlings on a degraded semiarid soil as influenced by mycorrhizal inoculation and sewage-sludge amendmentJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 5 2004María del Mar Alguacil Abstract A field experiment was carried out to evaluate the effectiveness of mycorrhizal inoculation with three arbuscular mycorrhizal (AM) fungi (Glomus intraradices Schenck & Smith, Glomus deserticola (Trappe, Bloss. & Menge), and Glomus mosseae (Nicol & Gerd.) Gerd. & Trappe) and the addition of composted sewage sludge (SS) with respect to the establishment of Retama sphaerocarpa L. seedlings, in a semiarid Mediterranean area. Associated changes in soil chemical (nutrient content and labile carbon fractions), biochemical (enzyme activities), and physical (aggregate stability) parameters were observed. Six months after planting, both the addition of composted SS and the mycorrhizal-inoculation treatments had increased total N content, available-P content, and aggregate stability of the soil. Values of water-soluble C and water-soluble carbohydrates were increased only in the mycorrhizal-inoculation treatments. Rhizosphere soil from the mycorrhizal-inoculation treatments had significantly higher enzyme activities (dehydrogenase, protease-BAA, acid phosphatase, and ,-glucosidase) than the control soil. In the short-term, mycorrhizal inoculation with AM fungi was the most effective treatment for enhancement of shoot biomass, particularly with G. mosseae (about 146% higher with respect to control plants). The addition of the composted SS alone was sufficient to restore soil structural stability but was not effective with respect to improving the performance of R. sphaerocarpa plants. Besiedlung eines degradierten semiariden Bodens mit Retama sphaerocarpa L.-Setzlingen, beeinflusst durch Mykorrhiza-Inokulation und Klärschlammzugabe Ein Feldversuch wurde durchgeführt, um den Effekt einer Inokulation mit drei arbuskulären Mykorrhizapilzen (AM) (Glomus intraradices Schenck & Smith, Glomus deserticola (Trappe, Bloss. & Menge) und Glomus mosseae (Nicol & Gerd.) Gerd. & Trappe) einerseits und der Zugabe von kompostiertem Klärschlamm (SS) andererseits auf die Besiedlung von Retama sphaerocarpa L.-Setzlingen in einem mediterranen semiariden Gebiet zu untersuchen. Es wurden chemischer Nährstoffgehalt, labile C-Fraktion, biochemische Enzymaktivitäten und physikalische Bodenparameter (Aggregatstabilität) untersucht. Sechs Monate nach der Pflanzung erbrachten beide Behandlungen , die Zugabe von kompostiertem Klärschlamm und die Mykorrhiza-Inokulation , Steigerungen des Gesamtstickstoff-Gehaltes, des verfügbaren Phosphor-Gehaltes sowie der Aggregatstabilität des Bodens. Wasserlöslicher Kohlenstoff und wasserlösliche Kohlenhydrate waren nur nach Mykorrhiza-Inokulation erhöht. Boden aus der Rhizosphäre, der mit Mykorrhizapilzen inokuliert wurde, zeigte signifikant höhere Enzymaktivitäten (Dehydrogenase, Protease-BAA, saure Phosphatase und ,-Glucosidase) als der Kontrollboden. In der kurzen Periode war die Inokulation mit AM-Pilzen die effektivste Behandlung bei der Bildung von Sprossbiomasse, speziell bei G. mosseae (eine um über 146,% höhere Biomasse im Vergleich zu den Kontrollpflanzen). Die Zugabe von kompostiertem Klärschlamm allein war ausreichend, die Stabilität der Bodenstruktur wiederherzustellen, aber sie war nicht effektiv hinsichtlich der Entwicklung der R. sphaerocarpa -Pflanzen. [source] Presubmergence and green manure affect the transformations of nitrogen-15-labeled urea under lowland soil conditionsJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 4 2003H.S. Thind Abstract The effect of presubmergence and green manuring on various processes involved in [15N]-urea transformations were studied in a growth chamber after [15N]-urea application to floodwater. Presubmergence for 14 days increased urea hydrolysis rates and floodwater pH, resulting in higher NH3 volatilization as compared to without presubmergence. Presubmergence also increased nitrification and subsequent denitrification but lower N assimilation by floodwater algae caused higher gaseous losses. Addition of green manure maintained higher NH4+ -N concentration in floodwater mainly because of lower nitrification rates but resulted in highest NH3 volatilization losses. Although green manure did not affect the KCl extractable NH4+ -N from applied fertilizer, it maintained higher NH4+ -N content due to its decomposition and increased mineralization of organic N. After 32 days about 36.9,% (T1), 23.9,% (T2), and 36.4,% (T3) of the applied urea N was incorporated in the pool of soil organic N in treatments. It was evident that the presubmergence has effected the recovery of applied urea N. No translation. [source] Soil biochemical and chemical changes in relation to mature spruce (Picea abies) forest conversion and regenerationJOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, Issue 3 2003Zheke Zhong Abstract To investigate soil changes from forest conversion and regeneration, soil net N mineralization, potential nitrification, microbial biomass N, L-asparaginase, L-glutaminase, and other chemical and biological properties were examined in three adjacent stands: mature pure and dense Norway spruce (Picea abies (L.) Karst) (110 yr) (stand I), mature Norway spruce mixed with young beech (Fagus sylvatica) (5 yr) (stand II), and young Norway spruce (16 yr) (stand III). The latter two stands were converted or regenerated from the mature Norway spruce stand as former. The studied soils were characterized as having a very low pH value (2.9 , 3.5 in 0.01 M CaCl2), a high total N content (1.06 , 1.94,%), a high metabolic quotient (qCO2) (6.7 , 16.9 g CO2 kg,1 h,1), a low microbial biomass N (1.1 , 3.3,% of total N, except LOf1 at stand III), and a relatively high net N mineralization (175 , 1213 mg N kg,1 in LOf1 and Of2, 4 weeks incubation). In the converted forest (stand II), C,:,N ratio and qCO2 values in the LOf1 layer decreased significantly, and base saturation and exchangeable Ca showed a somewhat increment in mineral soil. In the regenerated forest (stand III), the total N storage in the surface layers decreased by 30,%. The surface organic layers (LOf1, Of2) possessed a very high net N mineralization (1.5 , 3 times higher than those in other two stands), high microbial biomass (C, N), and high basal respiration and qCO2 values. Meanwhile, in the Oh layer, the base saturation and the exchangeable Ca decreased. All studied substrates showed little net nitrification after the first period of incubation (2 weeks). In the later period of incubation (7 , 11 weeks), a considerable amount of NO3 -N accumulated (20 , 100,% of total cumulative mineral N) in the soils from the two pure spruce stands (I, III). In contrast, there was almost no net NO3 -N accumulation in the soils from the converted mixed stand (II) indicating that there was a difference in microorganisms in the two types of forest ecosystems. Soil microbial biomass N, mineral N, net N mineralization, L-asparaginase, and L-glutaminase were correlated and associated with forest management. Chemische und biochemische Veränderungen der Bodeneigenschaften durch Verjüngung und Waldumbau eines Fichtenaltbestandes Um die durch den Waldumbau und die Regeneration bedingten Standortsveränderungen zu untersuchen, wurden die Netto-Stickstoffmineralisierung, die potenzielle Nitrifikation, der mikrobiell gebundene Stickstoff (Nmic), L-Asparaginase, L-Glutaminase sowie weitere chemische und biologische Parameter an drei benachbarten Standorten untersucht: Standort I, reiner Fichtenaltbestand (Picea abies (L.) Karst ,110 Jahre); Standort II, Fichtenaltbestand mit Buchenunterbau (Fagus sylvatica , 5 Jahre); Standort III, reine Fichtenaufforstung (16 Jahre). Die Standorte II und III entstanden infolge des Waldumbaus aus reinen Fichtenaltbeständen. Die untersuchten Böden sind gekennzeichnet durch sehr niedrige pH-Werte (pH(H2O) 3, 7 , 4, 2, pH (CaCl2) 2, 9 , 3, 5), hohe Gesamtstickstoffgehalte (1, 06 , 1, 94,%), hohe metabolische Quotienten (6, 7,16, 9g CO2 kg,1 h,1), geringe Nmic -Gehalte (1, 1 , 3, 3,% des Gesamt-N, ausgenommen LOf1 von Standort III) und eine relativ hohe N-Nettomineralisation (175 , 1213 mg N Kg,1 in LOf1 und Of2, nach 4 Wochen Inkubation). Am Standort II nahm das C,:,N-Verhältnis und der qCO2 im LOf1 -Horizont deutlich ab, wohingegen der Gehalt an austauschbarem Ca sowie die Basensättigung im Mineralboden geringfügig zunahmen. Am Standort III nahm der N-Vorrat (Auflagehumus + Mineralboden 0 , 10,cm) um 30,% ab. In den LOf1 - und Of2 -Lagen des Auflagehumus dieses Standortes traten eine hohe N-Nettomineralisation (1, 5- bis 3fach höher als in den Standorten I und II), hohe Gehalte an mikrobiell gebundenem C und N, eine erhöhte Basalatmung sowie erhöhte qCO2 -Werte auf. In den Oh-Lagen hingegen nahm die Basensättigung ab. Alle untersuchten Standorte zeigten in der ersten Periode der Inkubation (0 bis 2 Wochen) eine geringe Netto-Nitrifikation. An den Standorten I und III fand in der späteren Periode (7. bis 11. Woche) eine Anreicherung an NO3 (20 , 100,% des gesamten mineralischen N-Vorrates) statt. Im Gegensatz dazu wurde am Standort II keine NO3 -N- Anreicherung festgestellt. Dies deutet auf einen Unterschied in der Zusammensetzung der mikrobiellen Gemeinschaften in den zwei verschiedenen Forstökosystemen hin. Nmic, N-Nettomineralisation, L-Asparaginase und L-Glutaminase korrelieren miteinander und zeigen eine enge Beziehung zu den Bewirtschaftungsformen. [source] Preliminary study on the use of near-infrared reflectance spectroscopy to assess nitrogen content of undried wheat plantsJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 1 2007Alejandro Morón Abstract Near-infrared reflectance (NIR) spectroscopy combined with chemometrics was used to assess nitrogen (N) and dry matter content (DM) and chlorophyll in whole-wheat plant (Triticum aestivum L). Whole-wheat plant samples (n = 245) were analysed by reference method and by visible and NIR spectroscopy, in fresh (n = 182) and dry (n = 63) presentations, respectively. Calibration equations were developed using partial least squares (PLS) and validated using full cross-validation (leave-one-out method). Coefficient of determination in calibration (R2CAL) and the standard error of cross-validation (SECV) for N content in fresh sample presentation, after second derivative, were 0.89 (SECV: 0.64%), 0.86 (SECV: 0.66%) and 0.82 (SECV: 0.74%) using the visible + NIR, NIR and visible wavelength regions, respectively. Dry sample presentation gave better R2CAL and SECV for N compared with fresh presentation (R2CAL > 0.90, SECV < 0.20%) using visible + NIR. The results demonstrated that NIR is a suitable method to assess N concentration in wheat plant using fresh samples (unground and undried). Copyright © 2006 Society of Chemical Industry [source] Functional traits of woody plants: correspondence of species rankings between field adults and laboratory-grown seedlings?JOURNAL OF VEGETATION SCIENCE, Issue 3 2003J.H.C. Cornelissen Castroviejo et al. (1986,2000); Stace (1991) Abstract. Research into interspecific variation in functional traits is important for our understanding of trade-offs in plant design and function, for plant functional type classifications and for understanding ecosystem responses to shifts in species composition. Interspecific rankings of functional traits are a function of, among other factors, ontogenetic or allometric development and environmental effects on phenotypes. For woody plants, which attain large size and long lives, these factors might have strong effects on interspecific trait rankings. This paper is the first to test and compare the correspondence of interspecific rankings between laboratory grown seedlings and field grown adult plants for a wide range of functional leaf and stem traits. It employs data for 90 diverse woody and semiwoody species in a temperate British and a (sub)Mediterranean Spanish flora, all collected according to a strict protocol. For 12 out of 14 leaf and stem traits we found significant correlations between the species ranking in laboratory seedlings and field adults. For leaf size and maximum stem vessel diameter > 50 % of variation in field adults was explained by that in laboratory seedlings. Two important determinants of plant and ecosystem functioning, specific leaf area and leaf N content, had only 27 to 36 and 17 to 31 % of variation, respectively, in field adults explained by laboratory seedlings, owing to subsets of species with particular ecologies deviating from the general trend. In contrast, interspecific rankings for the same traits were strongly correlated between populations of field adults on different geological substrata. Extrapolation of interspecific trait rankings from laboratory seedlings to adult plants in the field, or vice versa, should be done with great caution. [source] Effect of young woody plantations on carbon and nutrient accretion rates in a redeveloping soil on coalmine spoil in a dry tropical environment, IndiaLAND DEGRADATION AND DEVELOPMENT, Issue 1 2006A. N. Singh Abstract We compared the effects of young high-density plantations of three native trees (legumes: Albizia lebbeck, A. procera and a non-legume: Tectona grandis) and one fast growing woody grass species (Dendrocalamus strictus) on carbon and nutrients stock and their accretion rates in a redeveloping soil. This soil was the early phase of mine spoil restoration in a dry tropical environment. The soil bulk density and accumulation rates of C, N and P at 0,10 and 10,20,cm soil depth were determined in 4- to 5-year-old plantations. The total nutrient stock of soil C, N, P significantly varied in redeveloping soil according to plantation type, plantation age and soil depth. A. lebbeck greatly improved C and N content followed by D. strictus, A. procera and T. grandis plantations. However, accretion rates of C and N were substantially high in the D. strictus plantation. Therefore, D. strictus, contributed significantly to the redevelopment of mine spoil soils. In the case of total P nutrient, A. procera showed the greatest amount among the plantations but the accretion rate was also high for T. grandis followed by A. procera, A. lebbeck and D. strictus. This study indicates that all N-fixing species may not be equally efficient in improving soil qualities especially N in the soil. Copyright © 2006 John Wiley & Sons, Ltd. [source] New Cyclic Olefin Copolymer for the Preparation of Thermally Responsive Luminescent FilmsMACROMOLECULAR CHEMISTRY AND PHYSICS, Issue 9 2009Filippo Donati Abstract Polymer films with optical properties as indicators to thermal stress were obtained through the controlled dispersion of moderate amounts (,0.1 wt.-%) of the 4,4,-bis(2-benzoxazolyl)stilbene (BBS) dye into a new semicrystalline ethylene-norbornene (E - co - N, N content of 15.3 mol.-%) copolymer characterized by a glass transition temperature (Tg) of about 64,°C. All the pristine films showed optical characteristics coming from noninteracting BBS chromophores. In contrast permanent optical changes were detected after film annealing at a T,,,Tg due to the variation of the BBS supramolecular structure. The easy modulation of the optical features of polymer films by thermal perturbations suggests applications as threshold temperature visual indicators in thermoplastic materials. [source] Silyl-Terminated Ethylene- co -Norbornene Copolymers by Organotitanium-Based CatalystsMACROMOLECULAR RAPID COMMUNICATIONS, Issue 1 2009Roberto Marconi Abstract Ethylene (E) and norbornene (N) were copolymerized in the presence of PhSiH3 as chain-transfer agent with [Ti(,5:,1 -C5Me4SiMe2NBut)(,1 -Me)2] precatalyst combined with [Ph3C][B(C6F5)4]. The silane was introduced at chain-ends of E- co -N copolymers with concomitant reinitiation of the growing polymer chain. The concentrations of the silane and polymer molecular weight are inversely correlated. The characteristic signals of SiH2Ph chain-ends were observed by 1H NMR. The Si heteroatom is predominantly adjacent to ethylene units in E- co -N copolymers with high N content. [source] Nutrient limitation and morphological plasticity of the carnivorous pitcher plant Sarracenia purpurea in contrasting wetland environmentsNEW PHYTOLOGIST, Issue 3 2008Terry Bott Summary ,,Plasticity of leaf nutrient content and morphology, and macronutrient limitation were examined in the northern pitcher plant, Sarracenia purpurea subsp. purpurea, in relation to soil nutrient availability in an open, neutral pH fen and a shady, acidic ombrotrophic bog, over 2 yr following reciprocal transplantation of S. purpurea between the wetlands. ,,In both wetlands, plants were limited by nitrogen (N) but not phosphorus (P) (N content < 2% DW,1, N : P < 14) but photosynthetic quantum yields were high (FV/FM > 0.79). Despite carnivory, leaf N content correlated with dissolved N availability to plant roots (leaf N vs , r2 = 0.344, P < 0.0001); carnivorous N acquisition did not apparently overcome N limitation. ,,Following transplantation, N content and leaf morphological traits changed in new leaves to become more similar to plants in the new environment, reflecting wetland nutrient availability. Changes in leaf morphology were faster when plants were transplanted from fen to bog than from bog to fen, possibly reflecting a more stressful environment in the bog. ,,Morphological plasticity observed in response to changes in nutrient supply to the roots in natural habitats complements previous observations of morphological changes with experimental nutrient addition to pitchers. [source] What are the effects of nitrogen deficiency on growth components of lettuce?NEW PHYTOLOGIST, Issue 3 2000M. R. BROADLEY Relationships between nitrogen (N) content and growth are routinely measured in plants. This study determined the effects of N on the separate morphological and physiological components of plant growth, to assess how N-limited growth is effected through these components. Lettuce (Lactuca sativa) plants were grown hydroponically under contrasting N-supply regimes, with the external N supply either maintained continuously throughout the period of study, or withdrawn for up to 14 d. Richards' growth functions, selected using an objective curve-fitting technique, accounted for 99.0 and 99.1% of the variation in plant dry weight for control and N-limited plants respectively. Sublinear relationships occurred between N and relative growth rates under restricted N-supply conditions, consistent with previous observations. There were effects of treatment on morphological and physiological components of growth. Leaf weight ratio increased over time in control plants and decreased in N- limited plants. Shoot:root ratio followed a similar pattern. On a whole-plant basis, assimilation of carbon decreased in N-limited plants, a response paralleled by differences in stomatal conductance between treatments. Changes in C assimilation, expressed as a function of stomatal conductance to water vapour, suggest that the effects of N limitation on growth did not result directly from a lack of photosynthetic enzymes. Relationships between plant N content and components of growth will depend on the availability of different N pools for remobilization and use within the plant. [source] Linking above-ground and below-ground effects in autotrophic microcosms: effects of shading and defoliation on plant and soil propertiesOIKOS, Issue 3 2000Juha Mikola Although factors affecting plant growth and plant carbon/nutrient balance , e.g., light availability and defoliation by herbivores , may also propagate changes in below-ground food webs, few studies have aimed at linking the above-ground and below-ground effects. We established a 29-week laboratory experiment (,one growing season) using autotrophic microcosms to study the effects of light and defoliation on plant growth, plant carbon/nutrient balance, soil inorganic N content, and microbial activity and biomass in soil. Each microcosm contained three substrate layers , mineral soil, humus and plant litter , and one Nothofagus solandri var. cliffortioides seedling. The experiment constituted of the presence or absence of two treatments in a full factorial design: shading (50% decrease in light) and artificial defoliation (approximately 50% decrease in leaf area in the beginning of the growing season). At the end of the experiment a range of above-ground and below-ground properties were measured. The shading treatment reduced root and shoot mass, root/shoot ratio and leaf production of the seedlings, while the defoliation treatment significantly decreased leaf mass only. Leaf C and N content were not affected by either treatment. Shading increased NO 3,N concentration and decreased microbial biomass in humus, while defoliation did not significantly affect inorganic N or microbes in humus. The results show that plant responses to above-ground treatments have effects which propagate below ground, and that rather straightforward mechanisms may link above-ground and below-ground effects. The shading treatment, which reduced overall seedling growth and thus below-ground N use and C allocation, also led to changes in humus N content and microbial biomass, whereas defoliation, which did not affect overall growth, did not influence these below-ground properties. The study also shows the carbon/nutrient balance of N. solandri var. cliffortioides seedlings to be highly invariant to both shading and defoliation. [source] Investigating the in situ degradation of atrazine in groundwater,PEST MANAGEMENT SCIENCE (FORMERLY: PESTICIDE SCIENCE), Issue 4 2006Robert Pearson Abstract This study focused on whether or not atrazine could be degraded by indigenous groundwater bacteria as part of an in situ remediation approach. Groundwater was taken from an unconfined middle upper chalk site where concentrations of atrazine and nitrate were typically in the ranges 0.02,0.2 µg litre,1 and 11.6,25.1 mg NO3 -N litre,1 respectively. Sacrificial batch studies were performed using this groundwater spiked with atrazine at a concentration of 10 µg litre,1 in conjunction with a minimal mineral salts liquid (Glu-MMSL) medium which contained glucose as the sole carbon source. Treatments comprised either the Glu-MMSL groundwater cultured bacteria or Pseudomonas sp. strain ADP. Results from sacrificial batches indicated the occurrence of bacterial growth and denitrification as monitored by optical density (absorbance at 600 nm) and NO3 -N content. Analysis of atrazine content by solid phase extraction coupled with high-performance liquid chromatography showed no degradation of atrazine over a period of 103 days in either treatment. These results indicated that no acclimatised bacterial community featuring positive degraders to the herbicide atrazine had become established within this chalk aquifer in response to the trace levels encountered. Copyright © 2006 Society of Chemical Industry [source] MBE growth of InAsN on (100) InAs substratesPHYSICA STATUS SOLIDI (B) BASIC SOLID STATE PHYSICS, Issue 6 2005V. Sallet Abstract The growth of InAsN on (100) InAs substrates by molecular beam epitaxy is investigated. The incorporation of nitrogen in the alloy is compared with the case of GaAsN grown on GaAs. Under the same conditions, lower nitrogen contents are systematically measured in InAsN. In both cases, nitrogen incorporation was found to be enhanced with the decrease of the arsenic flux supplied to the surface, whereas the nitrogen concentrations measured in InAsN and GaAsN as a function of the growth temperature follow opposite tendencies. For GaAsN, the N content slightly increases as the growth temperature raises from 360 °C to 460 °C. On the contrary, for InAsN alloys, no significant amount of N is observed over the range 420,460 °C and a decrease of the growth temperature down to 370 °C is required to incorporate 1% of nitrogen in InAsN. The comparison of formation enthalpies of binary constituents enables to understand these behaviours. A following discussion addresses the local environment of N atoms in GaInAsN materials. (© 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Seasonal variations in nitrate reductase activity and internal N pools in intertidal brown algae are correlated with ambient nitrate concentrationsPLANT CELL & ENVIRONMENT, Issue 6 2007ERICA B. YOUNG ABSTRACT Nitrogen metabolism was examined in the intertidal seaweeds Fucus vesiculosus, Fucus serratus, Fucus spiralis and Laminaria digitata in a temperate Irish sea lough. Internal NO3 - storage, total N content and nitrate reductase activity (NRA) were most affected by ambient NO3 - , with highest values in winter, when ambient NO3 - was maximum, and declined with NO3 - during summer. In all species, NRA was six times higher in winter than in summer, and was markedly higher in Fucus species (e.g. 256 ± 33 nmol NO3 - min,1 g,1 in F. vesiculosus versus 55 ± 17 nmol NO3 - min,1 g,1 in L. digitata). Temperature and light were less important factors for N metabolism, but influenced in situ photosynthesis and respiration rates. NO3 - assimilating capacity (calculated from NRA) exceeded N demand (calculated from net photosynthesis rates and C : N ratios) by a factor of 0.7,50.0, yet seaweeds stored significant NO3 - (up to 40,86 µmol g,1). C : N ratio also increased with height in the intertidal zone (lowest in L. digitata and highest in F. spiralis), indicating that tidal emersion also significantly constrained N metabolism. These results suggest that, in contrast to the tight relationship between N and C metabolism in many microalgae, N and C metabolism could be uncoupled in marine macroalgae, which might be an important adaptation to the intertidal environment. [source] Adjustment of leaf photosynthesis to shade in a natural canopy: reallocation of nitrogenPLANT CELL & ENVIRONMENT, Issue 3 2005H. EICHELMANN ABSTRACT The present study was performed to investigate the adjustment of the constituents of the light and dark reactions of photosynthesis to the natural growth irradiance in the leaves of an overstorey species, Betula pendula Roth, a subcanopy species Tilia cordata P. Mill., and a herb Solidago virgaurea L. growing in a natural plant community in Järvselja, Estonia. Shoots were collected from the site and properties of individual leaves were measured in a laboratory, by applying a routine of kinetic gas exchange and optical measurements that revealed photosystem II (PSII), photosystem I (PSI), and cytochrome b6f densities per leaf area and the distribution of excitation (or chlorophyll, Chl) between the two photosystems. In parallel, N, Chl and ribulose-bisphosphate carboxylase-oxygenase (Rubisco) content was measured from the same leaves. The amount of N in photosynthetic proteins was calculated from the measured contents of the components of the photosynthetic machinery. Non-photosynthetic N was found as the residual of the budget. Growth in shade resulted in the decrease of leaf dry mass to a half of the DW in sun leaves in each species, but the total variation, from the top to the bottom of the canopy, was larger. Through the whole cross-section of the canopy, leaf dry weight (DW) and Rubisco content per area decreased by a factor of four, N content by a factor of three, but Chl content only by a factor of 1.7. PSII density decreased by a factor of 1.9, but PSI density by a factor of 3.2. The density of PSI adjusted to shade to a greater extent than the density of PSII. In shade, the distribution of N between the components of the photosynthetic machinery was shifted toward light-harvesting proteins at the expense of Rubisco. Non-photosynthetic N decreased the most substantially, from 54% in the sun leaves of B. pendula to 11% in the shade leaves of T. cordata. It is concluded that the redistribution of N toward light-harvesting Chl proteins in shade is not sufficient to keep the excitation rate of a PSII centre invariant. Contrary to PSII, the density of PSI , the photosystem that is in immediate contact with the carbon assimilation system , shade-adjusts almost proportionally with the latter, whereas its Chl antenna correspondingly increases. Even under N deficiency, a likely condition in the natural plant community, a substantial part of N is stored in non-photosynthetic proteins under abundant irradiation, but much less under limiting irradiation. At least in trees the general sequence of down-regulation due to shade adjustment is the following: (1) non-protein cell structures and non-photosynthetic proteins; (2) carbon assimilation proteins; (3) light reaction centre proteins, first PSI; and (4) chlorophyll-binding proteins. [source] Reduction of Botrytis cinerea sporulation in sweet basil by altering the concentrations of nitrogen and calcium in the irrigation solutionPLANT PATHOLOGY, Issue 4 2006U. Yermiyahu Current measures for suppressing grey mould (Botrytis cinerea) on sweet basil rely on fungicide applications and climate control. In this study, the nitrogen and calcium concentrations in the standard irrigation solution (7·2 and 1·3 mm, respectively) were varied in a series of experiments conducted in pots and large containers. Treatments differed in the N or Ca concentrations supplied, and effects on host response were determined in detached stem segments, individual plants and plots. Effects of the two elements on grey mould incidence, lesion size and rate of disease progression were erratic and rarely significant, but decreasing the concentration of N and increasing the concentration of Ca in the irrigation solution decreased sporulation of B. cinerea. In some cases, however, altering the standard irrigation solution resulted in significantly lower yields. Analyses of mineral contents in the shoots suggested that yields were not impaired when N content in the shoots was not reduced below 2·9% and Ca content was not raised above 1·3%. These findings were used to compose a modified irrigation solution containing half of the N (3·6 mm) and double of the Ca (2·55 mm) concentrations in the standard irrigation solution. The modified irrigation solution significantly reduced sporulation of B. cinerea without affecting crop yield. [source] | ||||||||||||||||||||||||||||||||||||||||