Home About us Contact
|
Home About us Contact | ||
|
|
||
Multiple-locus Variable-number Tandem-repeat Analysis (multiple-locu + variable-number_tandem-repeat_analysis)
Selected AbstractsDiversity of Escherichia coli O157 in a longitudinal farm study using multiple-locus variable-number tandem-repeat analysisJOURNAL OF APPLIED MICROBIOLOGY, Issue 5 2008A.M. Urdahl Abstract Aims:, To perform a longitudinal study of the diversity of Escherichia coli O157 from a ruminant pasture/stream environment using multiple-locus variable-number tandem-repeat analysis (MLVA). Methods and Results:, Samples of faecal droppings from grazing ruminants and from an adjacent stream were tested longitudinally for E. coli O157 by enrichment and immunomagnetic separation (IMS). Using MLVA, 24 different profiles were identified from a total of 231 E. coli O157 isolates, of which 80 were included in a similarity analysis. Four main clusters with several subclusters were observed. Although there was close contact between sheep and cattle during the study period, E. coli O157 was surprisingly not detected from cattle faeces. Conclusions:, The cluster analysis indicated both unrelated and closely related E. coli O157 strains. The choice of loci to target in MLVA is important for the subtyping result, as loci with high diversities are essential for discriminating between closely related isolates. Significance and Impact of the Study:, There is a lack of data available on the use of MLVA to describe E. coli O157 diversity and changes over time in the animal reservoirs and the environment. Such data are needed in order to further develop MLVA as a typing method. [source] Genotypic characterization of hospital Enterococcus faecalis strains using multiple-locus variable-number tandem-repeat analysisLETTERS IN APPLIED MICROBIOLOGY, Issue 1 2009E. Wa, ecka Abstract Aims:, The level of genetic diversity and relationships between the specific genotypes and the distribution of virulence determinants among Enterococcus faecalis strains isolated from patients hospitalized in different wards of two hospitals were investigated. Methods and Results:, Fifty-six clinical strains of E. faecalis, isolated from patients hospitalized in the period of 1999,2004 in several wards in Wroc,aw (Poland), were analysed by multiple-locus variable-number tandem-repeat analysis (MLVA). Analysis of seven genomic loci identified 40 novel genotypes among the analysed E. faecalis strains, with two major genomic groups, designated I and II, distinguished at a cut-off of 35%. With a similarity cut-off of 85·7%, the genotypes could be combined into 12 clusters (C1,C12), containing at least two isolates. The remaining 18 MLVA types were represented by a single isolate. Conclusions:, Based on the data obtained by MLVA, it was found that (i) many E. faecalis isolates recovered from patients from the wards whose location allowed the potential transmission of micro-organisms, belonged to closely related MLVA types and (ii) possible relationships between specific E. faecalis genotype and the virulence factors lipase, haemolysin and esp gene can exist. Significance and Impact of the Study:, Our study confirms that MLVA is a suitable method for the epidemiological study of E. faecalis and for the first time shows possible relationships between specific genotypes and such virulence determinants, i.e. lipase, haemolysin and esp gene. [source] | ||||||||||