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Morphological Observations (morphological + observation)
Selected AbstractsInfluences of Cross Pollination on Pollen Tube Growth and Fruit Set in Zuili Plums (Prunus salicina)JOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 2 2008Hui-Juan Jia Abstract Zuili plum (Prunus salicina L.) trees usually set fruit poorly, although they produce high quality fruit. To elucidate the causes of the poor fruit set, pollen tube growth into pistils and fruit set percentage were investigated after cross-, self- and open-pollination. Ovule development in Zuili pistils was also investigated. Pollen tube penetration into the ovules via the obturator and micropyle was best when Zuili pistils were pollinated by cv. Black Amber (P. domestica) pollen grains, although cross-pollinations with Hongxinli and Miili (P. salicina) pollen were more effective than self- and open-pollination. The fruit set percentage was also highest in pistils pollinated with Black Amber pollen grains. Morphological observation of Zuili pistils revealed that the trees produce "double pistils", developing two ovaries from a basal pistil, at a rate as high as 28%. In such abnormal pistils, most ovules were lacking an embryo sac or were entirely degenerated. The percentage of normally developed ovules was 24.3% and 8.9% in normal and double pistils, respectively. From these results, we conclude that the main causes of poor fruit set of Zuili plums are a lack of effective cross-pollination and the production of high percentages of double pistils in which normally developed ovules are scarcely formed. [source] Spatio-temporal distribution of cellular retinoid binding protein gene transcripts in the developing and the adult cochlea.EUROPEAN JOURNAL OF NEUROSCIENCE, Issue 8 2000CRBPI-null mutant mice, Morphological, functional consequences in CRABP- Abstract The expression patterns of the mouse cellular retinoid binding protein genes were investigated by in situ hybridization analysis in the inner ear from 10.5 days post coïtum (dpc) up to the adult stage. The cellular retinoic acid binding protein II (CRABPII) and cellular retinol binding protein I (CRBPI) were present in a widespread and abundant pattern in cochlear structures during embryogenesis. Expression of the cellular retinoic acid binding protein I (CRABPI) is restricted during development in Kölliker's organ whilst cellular retinol binding protein II (CRBPII) is only visible after birth with a ubiquitous distribution in most regions of the cochlea including nervous components. No CRABP or CRBP transcripts were observed in the auditory receptors. Morphological observations of CRBPI- and CRABPI/CRABPII-null mutant fetus at 18.5 dpc do not show any structural modification at the level of the organ of Corti. Furthermore, electrophysiological tests performed by measuring distorsion-product otoacoustic emissions and auditory brainstem evoked responses did not present significant alteration of the auditory function for the different types of mutants. The expression of retinoid binding proteins in cochlear structures during embryogenesis could suggest important roles for these proteins during ontogenesis and morphogenesis of the inner ear. Despite these observations, morphological and functional data from mutant mice did not present obvious modifications of the cochlear structures and auditory thresholds. It is therefore unlikely that CRABPs and CRBPI are directly involved in development of the cochlea and hair cell differentiation. [source] Rotationally molded polyethylene: Structural characterization by x-ray and microhardness measurementsADVANCES IN POLYMER TECHNOLOGY, Issue 2 2001Maria Clara Cramez Rotationally molded polyethylene (PE) blended in two ways (turbo blending and extrusion) with nucleating and nonnucleating pigments is structurally characterized by wide- and small-angle x-ray scattering (WAXS and SAXS, respectively), DSC and microhardness measurements. Morphological observations are performed by polarized light microscopy. The melting temperature and the degree of crystallinity (from both DSC and WAXS) remain essentially constant regardless of sample preparation and type of pigment. The same holds for the crystal sizes from WAXS and the lamella thickness from SAXS. Only the values of microhardness depend on the type of pigment, increasing about 10% when a nucleating type is used. The almost constant values of these properties, contrasting to the spherulitic morphology, are explained by the fact that the processing conditions in rotational molding are very favorable for crystallization. As a consequence, optimal crystalline structure is achieved, which masks significantly the effect of pigments and blending conditions on the crystallization behavior of polyethylene. © 2001 John Wiley & Sons, Inc. Adv Polym Techn 20: 116,124, 2001 [source] Characterization of apoptosis induced by grouper iridovirus in two newly established cell lines from barramundi, Lates calcarifer (Bloch)JOURNAL OF FISH DISEASES, Issue 11 2008Y-S Lai Abstract Two new cell lines have been established from the muscle and swim bladder tissues of barramundi, Lates calcarifer, and designated as BM (barramundi muscle) and BSB (barramundi swimbladder), respectively. The cells multiplied well at 28 °C in Leibovitz's L-15 medium supplemented with 10% foetal bovine serum, and have been continuously subcultured more than 100 times to date. Morphologically, BM cells were mostly fibroblastic, whereas BSB were mostly epithelial. Both cell lines were susceptible to grouper iridovirus (GIV) and displayed characteristics of apoptosis after viral infection. The induction of apoptosis was further assayed in GIV-infected BM and BSB cells by various methods. The inhibition of cell growth by GIV was demonstrated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Morphological observations revealed typical apoptotic features in the infected cells, including cell shrinkage and rounding, chromosome condensation and formation of apoptotic body-like vesicles. Chromosome fragmentation was detected by DNA laddering and TUNEL assays. Finally, the appearance of phosphotidylserine on the outer leaflet of apoptotic cell membranes was confirmed by annexin V staining. This is the first report of apoptosis induced by GIV in fish cells. [source] First record of Asteronema rhodochortonoides (Phaeophyceae) for the Pacific OceanPHYCOLOGICAL RESEARCH, Issue 4 2001Kazuhiro Kogame SUMMARY Morphological observations of a minute, filamentous, branched brown alga epiphytic on Sargassum thun-bergii (Mertens ex Roth) Kuntze were made on material collected at Tsuyazaki (33°48,N, 130°27,E), Fukuoka Prefecture, southern Japan. This alga was assignable to Asteronema rhodochortonoides (Børgesen) Möller et Parodi in having stellately arranged chloroplasts with several pyrenoids grouped in the center, predominantly apical growth, narrow filaments, and elliptical or broadly elliptical plurilocular zoidangia that are apically or laterally formed on upright filaments. A comparison of partial nuclear small subunit rDNA sequences between the Japanese material and A. rhodochortonoides from the Canary Islands showed only two or three nucleotide differences. This supports our assignment of the Japanese material to this species as a first report for the Pacific Ocean. In laboratory cultures, zoids released from plurilocular zoidangia developed into plants with morphology similar to the field-collected plants. This cycle repeated without production of unilocular zoidangia in our cultures. [source] Morphology and thermal properties of a PC/PE blend with reactive compatibilizationPOLYMERS FOR ADVANCED TECHNOLOGIES, Issue 6 2007Bo Yin Abstract Reactive compatibilization of immiscible polymers is becoming increasingly important and hence a representative study of a polycarbonate/high density polyethylene (PC/HDPE) system is the focus of this paper. A grafted copolymer PC- graft -ethylene- co -acrylic acid (PC- graft -EAA) was generated as a compatibilizer in situ during processing operation by ester and acid reaction between PC and ethylene-acrylic acid (EAA) in the presence of the catalyst dibutyl tin oxide (DBTO). As the polyethylene (PE) matrix does not play any part during the synthesis of the copolymer and since PC and EAA are also immiscible, to simplify the system, the influence of this copolymer formation at the interface between PC and EAA on rheological properties, phase morphology, and crystallization behavior for EAA/PC binary blends was first studied. The equilibrium torque increased with the DBTO content increasing in EAA/PC blends on Haake torque rheometer, indicating the in situ formation of the graft copolymer. Scanning electron microscopy (SEM) studies of cryogenically fractured surfaces showed a significant change at the distribution and dispersion of the dispersed phase in the presence of DBTO, compared with the EAA/PC blend without the catalyst. Differential scanning calorimetry (DSC) studies suggested that the heat of fusion of the EAA phase in PC/EAA blends with or without DBTO reduced with the formation of the copolymer compared with pure EAA. Then morphological studies and crystallization behavior of the uncompatibilized and compatibilized blends of PC/PE were studied as functions of EAA phase concentration and DBTO content. Morphological observations in PC/PE blends also revealed that on increasing the EAA content or adding the catalyst DBTO, the number of microvoids was reduced and the interface was intensive as compared to the uncompatibilized PC/PE blends. Crystallization studies indicated that PE crystallized at its bulk crystallization temperature. The degree of crystallinity of PE phase in PC/PE/EAA blends was also reduced with the addition of EAA and DBTO compared to the uncompatibilized blends of PC/PE, indicating the decrease in the degree of crystallinity was more in the presence of PC- graft -EAA. Copyright © 2007 John Wiley & Sons, Ltd. [source] A revision of Cerdia (Caryophyllaceae)BOTANICAL JOURNAL OF THE LINNEAN SOCIETY, Issue 1 2006VICTORIA SOSA Cerdia is a poorly understood genus of minute cushiony plants endemic to Mexico that is traditionally placed in subfamily Paronychioideae, tribe Polycarpeae (Caryophyllaceae). Morphological observations and phylogenetic analysis of ITS DNA sequences were carried out to determine the number of species, heretofore controversial, that constitute the genus as well as its position within Caryophyllaceae. Samples of Cerdia were collected through its entire range of distribution and together with previous specimens were used in morphological analyses. Nineteen terminal taxa were considered in a phylogenetic analysis with representatives of the different lineages in Caryophyllaceae. Results indicated that Cerdia is a monotypic genus, including only one variable species, C. virescens, but its phylogenetic affinities remain doubtful. © 2006 The Linnean Society of London, Botanical Journal of the Linnean Society, 2006, 152, 1,13. [source] Surface podzolization in Cambisols under deciduous forest in the Belgian loess beltEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 1 2000V. Brahy Summary Surface podzolization involves the migration of metal,humus complexes to a depth of a few centimetres. In acid soils derived from loess, this process has been diagnosed mainly by morphological observation. We investigated this process in a toposequence of Luvisols and Cambisols on loess using selective extraction and mineralogical data as well as characteristics of the leaf litter. The humus type (O and OAh horizons) is a moder in the three Luvisols and one of the Cambisols, whereas it is a fibrimor in the two other Cambisols. The contents in total alkaline and alkaline-earth cations range from 35 to 60 cmolc kg,1 in the fibrimor and from 40 to 90 cmolc kg,1 in the moder humus. In the two Cambisols with fibrimor smectite occurs in the clay fraction of the Ah horizon; Fe,humus complexes seem to have moved, but no more than 9 cm, from the Ah to the AB horizon beneath. Relative to the Ah horizon, the upper part of the AB has larger tetraborate-extractable Fe/Al ratio and optical density of the oxalate extract. Such features converge to diagnose surface podzolization in the Cambisols with fibrimor. However, they were not detected in the Cambisol and Luvisols with moder. In the two Cambisols with fibrimor, surface podzolization is consistent with (i) their smaller iron content, (ii) their more advanced weathering stage and (iii) their lower acid neutralizing capacity. [source] Essential oils from flowers of Centaurea kotschyi var. kotschyi and C. kotschyi var. decumbens from TurkeyFLAVOUR AND FRAGRANCE JOURNAL, Issue 2 2003Kuddisi Ertugrul Abstract The compositions of the essential oils obtained from fresh capitula of Centaurea kotschyi var. kotschyi and Centaurea kotschyi var. decumbens, two endemic plants from Turkey, have been studied. Sesquiterpenes were the main components of both essential oils, with germacrene D (44.2%), , -caryophyllene (12.1%) and bicyclogermacrene (5.5%) in C. kotschyi var. kotschyi and germacrene D (29.4%), , -caryophyllene (11.2%), , -cedrene (7.1%) in C. kotschyi var. decumbens as the principal components. The qualitative and quantitative differences between the two essential oils are in agreement with the morphological observation that led to the differentiation of the two varieties.Copyright © 2003 John Wiley & Sons, Ltd. [source] High-Turnover Periprosthetic Bone Remodeling and Immature Bone Formation Around Loose Cemented Total Hip JointsJOURNAL OF BONE AND MINERAL RESEARCH, Issue 1 2001Michiaki Takagi Abstract Aseptic loosening and periprosthetic osteolysis are the major problems awaiting solution in total hip surgery. The clinical investigation focused on the analysis of periprosthetic bone remodeling to clarify one important key event in the cascade of periprosthetic connective tissue weakening and osteolysis around loose artificial hip joints. Twelve acetabular bone samples adjacent to granulomatous synovial-like membrane of loose hip prosthesis were retrieved at revision surgery and processed for Villanueva bone staining for morphological observation and bone histomorphometric analysis. Eight well-fixed bony samples were used as control. Although osteoclastic surface and eroded surface by osteoclasts were evident in the periprosthetic bone from loose hip joints (p = 0.003 and p = 0.027), increased osteoid/low-mineralized bone matrix (p < 0.001) and osteoid width (p < 0.001) also were significant findings in structural analysis. In addition, not only elevated mineral apposition rate (MAR; p = 0.044) but also increased mineralizing surface (p = 0.044) and bone formation rate (BFR; p = 0.002) in loose periprosthetic bones were shown in dynamic data analysis. These results were confirmed by precise morphological observation by confocal laser scanning microscopy. Active coupling of bone formation and resorption and increased osteocytes with abundant bone canalicular projections were found in combined with the presence of immature bone matrices (osteoid and low-mineralized bone areas) in periprosthetic bones from loose hip joints. These results indicated that active osteoclastic bone resorption and/or defective bone formation are coupled with monocyte/macrophage-mediated foreign body-type granuloma in the synovial-like interface membrane of loose hip joints. Thus, this unique high-turnover periprosthetic bone remodeling with bad bone quality probably is caused by the result of cellular host response combined with inappropriate cyclic mechanical loading. The fragile periprosthetic bone may contribute to hip prosthesis loosening. [source] Phytophthora pinifolia sp. nov. associated with a serious needle disease of Pinus radiata in ChilePLANT PATHOLOGY, Issue 4 2008A. Durán During the course of the past three years, a new disease of Pinus radiata, referred to as ,Daño Foliar del Pino' (DFP) has appeared in the Arauco province of Chile and subsequently spread to other areas. The disease is typified by needle infections, exudation of resin at the bases of the needle brachyblasts and, in younger trees, necrotic lesions in the cambium, which eventually girdle the branches. The disease causes the death of young seedlings and mature trees can also succumb after a few years of successive infection, probably hastened by opportunistic fungi such as Diplodia pinea. Isolations on selective medium for Phytophthora spp. led to the consistent isolation of a Phytophthora sp. from needle tissue. DNA sequence comparisons for the ITS rDNA and cox II gene regions, and morphological observation showed that this oomycete represents a previously undescribed species for which the name Phytophthora pinifolia sp. nov. is provided. This new species is characterized by unbranched sporangiophores, and non-papillate, sub-globose to ovoid sporangia that are occasionally free from the sporangiophore with medium length pedicels. Despite using a number of oospore inducing techniques, oogonia/antheridia were not observed in isolates of P. pinifolia. Pathogenicity trials with P. pinifolia showed that it is pathogenic to P. radiata and causes rapid death of the succulent apical parts of young plants. Phytophthora pinifolia is the first Phytophthora known to be associated with needles and shoots of a Pinus sp. and its aerial habit is well matched with the occurrence and symptoms of DFP in Chile. [source] Effect of surface treatment for carbon nanotubes on morphological and rheological properties of poly(ethylene oxide) nanocompositesPOLYMER ENGINEERING & SCIENCE, Issue 10 2006Young S. Song Poly(ethylene oxide) nanocomposites filled with functionalized multi-walled carbon nanotubes are prepared and characterized using rheological and morphological measurements. This study investigates how the surface treatment of carbon nanotubes (CNTs) affects the CNT dispersion state. It is found that the nanocomposites have a higher effective volume fraction than the real volume fraction of the CNTs. The dispersion state of the CNTs is identified by using field emission scanning electron spectroscope and transmission electron microscope. The rheological findings indicate that there exists a percolated network structure of the CNTs in the nanocomposites, which was confirmed by electrical conductivity measurements as well as morphological observation. POLYM. ENG. SCI., 46:1350,1357, 2006. © 2006 Society of Plastics Engineers [source] Growth and characterization of FexMn1-xIn2Se4 (0 , x , 1) single crystalsCRYSTAL RESEARCH AND TECHNOLOGY, Issue 10-11 2005G. Attolini Abstract Single crystals of Mn1-xFexIn2Se4 with 0 , x , 1.0 were grown by chemical vapor transport technique using I2 as transporting agent. The resulting crystals appeared as brilliant black plates. X-ray powder diffraction data could be indexed following the hexagonal structure with space group Rm for the low Fe concentration samples, while the high concentration sample is associated to the space group P63mc. Both polytypes have similar parameters, however the c parameter reduces as the Fe concentration increases, therefore the number of anion slabs decreases. The characterization of the crystals was based on morphological observations and optical absorption as a function of temperature. The obtained energy gap for the samples at different Fe concentrations suggested that they behave as direct energy gap semiconductors. (© 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Snailfishes of the central California coast: video, photographic and morphological observationsJOURNAL OF FISH BIOLOGY, Issue 4 2006D. L. Stein Video and photographic images of snailfishes (Liparidae) collected by the Monterey Bay Aquarium Research Institute, augmented by specimens collected simultaneously, were analysed. Nine species in five genera were identified, including Careproctus melanurus, Careproctus ovigerus, Careproctus longifilis, Careproctus gilberti, Careproctus filamentosus, Osteodiscus cascadiae, Nectoliparis pelagicus, Paraliparis dactylosus and Rhinoliparis barbulifer. Voucher specimens were collected of all except C. melanurus, C. gilberti and C. filamentosus. In addition, individuals of the Paraliparis,rosaceus' species group were abundant but could not be identified to species. Many liparids were identified only to family, but an individual of a very distinctive unknown species, presumably undescribed, was videotaped. Relative abundance of C. melanurus was estimated, and several in situ snailfish behaviours are described for the first time. [source] INTER- AND INTRASPECIFIC VARIATION OF THE PSEUDO-NITZSCHIA DELICATISSIMA COMPLEX (BACILLARIOPHYCEAE) ILLUSTRATED BY RRNA PROBES, MORPHOLOGICAL DATA AND PHYLOGENETIC ANALYSES,JOURNAL OF PHYCOLOGY, Issue 2 2006Nina Lundholm A study of 25 cultures tentatively identified as Pseudo-nitzschia delicatissima (Cleve) Heiden, and originating from geographically widely distributed locations, showed both morphological and genetic variation among strains. Use of rRNA-targeted DNA probes on 17 different strains showed large variation in the hybridization patterns. Detailed morphological studies placed the isolates into three groups. The sample on which the neotype of P. delicatissima is based was also examined, and used to establish the morphological identity of P. delicatissima. Phylogenetic analyses of 16 strains, based on sequences of internal transcriber spacer 1 (ITS1), 5.8S and ITS2 of the nuclear-encoded rDNA, supported the morphological observations and the hybridization studies, and revealed large genetic variation among strains. A combination of the morphological and molecular findings resulted in the description of two new species, P. decipiens sp. nov. and P. dolorosa sp. nov. P. dolorosa has a mixture of one or two rows of poroids in the striae whereas P. delicatissima always has two rows. In addition, P. dolorosa has wider valves and a lower density of poroids. P. decipiens differs from P. delicatissima by a higher density of striae on the valve face as well as a higher density of poroids on the girdle bands. Among the strains referred to P. delicatissima, an epitype was selected. Large genetic variation was found among the P. delicatissima strains and a subdivision into two major clades represent cryptic species. [source] Correlation of morphology, rheology, and performance improvement in gasoline tubes based on PA-6 nanocompositesJOURNAL OF VINYL & ADDITIVE TECHNOLOGY, Issue 3 2010Mehdi Moghri PA-6/organo-modified layered silicate nanocompounds were prepared by the melt mixing of PA-6 with different nanoclay loadings in a corotating twin-screw extruder. Gasoline tubes based on these nanocompounds were produced at different silicate loadings. Thermal, mechanical, rheological, and barrier properties of the different samples were investigated and correlated to their morphology. Transmission electron microscopy, wide angle X-ray scattering, and linear melt state viscoelastic measurements were used to characterize the different aspects of nanoclay dispersion in the nanocomposite samples. While tensile modulus, softening point, heat distortion temperature, and gasoline barrier properties of the prepared tubes were improved considerably by increasing the clay content, performance improvement with respect to clay content (after a certain value) decreased with increasing clay loading. It could be attributed to the re-agglomeration of tactoids at higher concentrations. These findings were correlated with the rheological and morphological observations. J. VINYL ADDIT. TECHNOL., 2010. © 2010 Society of Plastics Engineers [source] Maleated polypropylene film and wood fiber handsheet laminates,POLYMER COMPOSITES, Issue 12 2009Sangyeob Lee The grafting effect of maleic anhydride (MA) as an interfacial bonding agent and its influence on the tensile strength properties of thermomechanical pulp handsheet-isotactic polypropylene (iPP) film laminates was studied. For the MA treated with benzoyl peroxide (BPO) as an initiator, tensile strength properties increased 76% with PP film over untreated laminates. The optimal strength properties were obtained with a MA and BPO ratio of 2:1. A strong correlation was observed between the number of fibers in the web and tensile strength properties for both handsheet drying conditions. The R2 values were 0.95 for air-dry conditions and 0.94 from oven-dry conditions. Scanning electron microscopy images also showed the effectiveness of MA loading on the surface of thermomechanical pulp fibers due to increased fiber failure, which occurred without fiber being pulled out from the PP matrixes. Crystallinity and heat flow were determined using differential scanning calorimetry (DSC) and increased as expected as the ratio of MA and BPO increased from 0:0 to 2:1. These results were also in accordance with the morphological observations at the fracture surface, Fourier transform infrared spectra, and thermal analysis. POLYM. COMPOS., 2009. © 2008 Society of Plastics Engineers [source] In vitro and in vivo antineoplastic activity of a novel bromopyrrole and its potential mechanism of actionBRITISH JOURNAL OF PHARMACOLOGY, Issue 4 2010Sheng Xiong Background and purpose:, Many bromopyrrole compounds have been reported to have in vitro antineoplastic activity. In a previous study, we isolated N-(4, 5-dibromo-pyrrole-2-carbonyl)-L-amino isovaleric acid methyl ester (B6) from marine sponges. Here, we investigated the in vitro and in vivo antineoplastic activity of B6 and its potential mechanism. Experimental approach:, The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to determine the in vitro antineoplastic activity of B6. Flow cytometry, western blot analysis and morphological observations were used to investigate its mechanism of action. A mouse xenograft model was used to determine its in vivo activity. Key results:, B6 inhibited the proliferation of various human cancer cells in vitro, with highest activity on LOVO and HeLa cells. B6 also exhibited significant growth inhibitory effects in vivo in a xenograft mouse model. Acute toxicity analysis suggested that B6 has low toxicity. B6-treated cells arrested in the G1 phase of the cell cycle and had an increased fraction of sub-G1 cells. In addition, the population of Annexin V-positive/propidium iodide-negative cells increased, indicating the induction of early apoptosis. Indeed, B6-treated cells exhibited morphologies typical of cells undergoing apoptosis. Western blotting showed cleaved forms of caspase-9 and caspase-3 in cells exposed to B6. Moreover, B6-promoted Ca2+ release and apoptosis was associated with elevated intracellular Ca2+concentration. Conclusions and implications:, B6 has significant antineoplastic activity in vitro as well as in vivo. It inhibits tumour cell proliferation by arresting the cell cycle and inducing apoptosis. With its low toxicity, B6 represents a promising antineoplastic, primary compound. [source] Gnathostomulid phylogeny inferred from a combined approach of four molecular loci and morphologyCLADISTICS, Issue 1 2006Martin V. Sørensen The phylogeny of the obscure metazoan phylum Gnathostomulida has previously only been addressed with cladistic analyses of morphological data. In the present study DNA sequence data from four molecular loci, including 18S rRNA, 28S rRNA, histone H3 and cytochrome c oxidase subunit I, are added to a revised morphological data matrix. The data set represents 23 gnathostomulid species that are analyzed under direct optimization using parsimony as the optimality criterion. The results obtained from analyzing the four molecular loci and combined morphological and molecular data under different parameter sets are generally very congruent, and differ only on minor points. The results clearly support gnathostomulid monophyly, as well as the basal division of Gnathostomulida into Filospermoidea and Bursovaginoidea. Filospermoidea were represented by species of Haplognathia and Cosmognathia, and generic monophyly is supported for both groups. Within Bursovaginoidea, Conophoralia (= Austrognathiidae) and Scleroperalia appear as sister groups. Monophyly of Mesognathariidae was confirmed as well, whereas the relationships between species of Gnathostomulidae and Onychognathiidae were contradicted by the molecular data when compared to morphological observations. ©The Willi Hennig Society 2006. 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