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Morphological Examination (morphological + examination)
Selected AbstractsMorphological Examination of the Intraorbital Muscles (Musculi Bulbi) in Dogs in the Perinatal PeriodANATOMIA, HISTOLOGIA, EMBRYOLOGIA, Issue 5 2006J. Kle, kowska Summary Twelve American Staffordshire terriers, gestational day 60, and 10 dogs de Bordeaux, gestational day 57 were examined in respect of the morphology and morphometry of their intraorbital muscles. The location of the retractor bulbi, recti and oblique muscles was described and the length of the muscles, the length and breadth of their tendons as well as the distance of the distal insertions of the muscle tendons from the corneal limbus were measured. Similarly, the shape of the line of distal insertions was investigated. The measurements were taken with an electronic caliper to the nearest 0.1 mm. The distance insertion,corneal limbus was measured by means of the Hifny and Misk method (1982). The following differences between the breeds in the morphometry of the intraorbital muscles and their tendons were found out. The distal insertions of the tendons of the dorsal, ventral and lateral recti muscles are further from the corneal limbus in American Staffordshire terriers than in dogs de Bordeaux. The muscular funiculi of the retractor bulbi muscle are further from the corneal limbus in dogs de Bordeaux, except the dorsolateral funiculus (1.96/1.94 mm). In addition, there are differences in the morphometry of the intraorbital muscles and their tendons. No differences, however, were found in the morphology of the intraorbital muscle tendons (their insertion line) and their location. The study can be applied to clinical sciences (surgery) and veterinary ophthalmology, in particular. [source] Functionalization of LDPE by Melt Grafting with Glycidyl Methacrylate and Reactive Blending with Polyamide-6MACROMOLECULAR CHEMISTRY AND PHYSICS, Issue 8 2003Qian Wei Abstract Low-density polyethylene (LDPE) was functionalized by melt radical grafting with glycidyl methacrylate (GMA) and employed for reactive blending with polyamide-6 (PA6). The effect of the reaction procedure on the grafting degree of LDPE- g -GMA samples (0.5,12.5 wt.-% GMA) was analyzed as a function of the concentration of GMA monomer, radical initiator (BTP), and addition of styrene as co-monomer. Optimized grafting conditions were obtained when the amount of the monomer is below 10 wt.-% and that of peroxide in the range 0.2,0.4 wt.-%. Binary blends of PA6 with LDPE- g -GMA (3.5 wt.-% GMA) and with LDPE at various compositions (80/20, 67/33, 50/50 wt.-%) were prepared in an internal mixer and their properties were evaluated by torque, SEM and DSC analyses. Morphological examination by SEM showed a large improvement of phase dispersion and interfacial adhesion in PA6/LDPE- g -GMA blends as compared with PA6/LDPE blends. The average diameter of dispersed polyolefin particles was about 0.4 ,m for LDPE- g -GMA contents <,50 wt.-%. A marked increase of melt viscosity was observed for the compatibilized blends depending on the concentration of grafted polyolefin, and it was accounted for by the reaction between the epoxy groups of GMA and the carboxyl/amine end-groups of PA6. The variation of torque was thus related to the molar ratio of reactive group concentration. The analysis of crystallization and melting behavior pointed out marked differences in the phase structure of the blends due to the presence of the functionalized polyolefin. Finally, the in situ formation of a graft copolymer between LDPE- g -GMA and PA6 was investigated by means of a selective dissolution method (Molau test) and by FT-IR and DSC analyses. SEM micrograph of fracture surface of PA6/LDPE- g -GMA 50/50 blend. [source] Evidence for the importance of odour-perception in the parasitoid Rhopalicus tutela (Walker) (Hym., Pteromalidae)JOURNAL OF APPLIED ENTOMOLOGY, Issue 6 2001E. M. Pettersson Possible host location mechanisms in the chalcid wasp Rhopalicus tutela (Walker) (Hym., Pteromalidae), a parasitoid of the eight-spined spruce bark beetle, Ips typographus (L.) (Col., Scolytidae), were examined. This was carried out in order to repeat and complement former studies on parallel parasitoid,scolytid systems that had contradictory results. Morphological examinations of the parasitoid antennae were made using both scanning and transmission electron microscopy. Possible functions of the sensilla placodea (multiporous plate sensillum), and other sensilla present on the antennae, have been indicated. For the first time, the placoid sensilla in a pteromalid parasitoid have shown porous walls and numerous innervations, which are typical characteristics for chemoreceptors. Previously the placoid sensilla have been suggested to be an infrared receptor. In order to test the chemoreceptive ability of R. tutela females and males, a synthetic reference blend was analysed by combined gas chromatography and electroantennographic detector (GC-EAD). Their sensitivity to host-related volatiles (such as certain pheromone components and oxygenated monoterpenes) was significantly greater than that for host-tree-related compounds (monoterpene hydrocarbons). Employing an infrared thermo-scanner, the current study failed to detect ,hot spots' associated with susceptible hosts beneath the bark. Results from electrophysiology and electron microscopy revealed clear odour-perceptive functions of the parasitoid antennae. These results strongly support the major importance of volatiles in host location by the bark beetle parasitoid R. tutela. [source] Prenatal developmental toxicity studies of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p, p,-DDT) in rats and rabbitsCONGENITAL ANOMALIES, Issue 4 2001Ken L. Takahashi ABSTRACT, The studies were conducted in rats and rabbits to elucidate the potential developmental toxicity of p, p'-DDT in general accordance with the improved Japanese MAFF guidelines (12-Nousan-No. 8147,2,1,18, 2000). p, p'-DDT suspended in 1% aqueous solution of CMC was administered orally to pregnant Jcl:SD rats on gestational days (GD) 6,19 at a dose of 0,5, 25, or 100 mg/kg/day and to pregnant KbI: JW rabbits on GD 6,27 at a dose of 0,5,20, or 80 mg/kg/day. Maternal animals were killed on the day after the last day of administration for morphological examination of their fetuses with special attention to the reproductive organs. Adverse effects on maternal animals were found only at the highest dose in both species; i.e., clonic convulsion (2/24 in rats, 5/22 in rabbits), mortality (1/24 in rats), abortion or premature delivery (4/22 in rabbits), and reduced body weight gains and food consumption. However, the control and treated groups showed comparable values for the numbers of corpora lutea and implants, percent preimplantation losses, number of live fetuses, percent resorptions and fetal deaths, sex ratio, fetal body weights, and placental weights in both species, and anogenital distance and testicular histology in rats. Although fetal examination revealed slightly increased incidence of 27 presacral vertebrae in the highest dose group in rats, there was no treatment-related increase in the incidence of malformations in any of the species. Based on these results, it is concluded that p, p'-DDT causes no malformations, including male reproductive organ abnormalities, in either rats or rabbits, although it results in an increased incidence of skeletal variations in rats at a maternally toxic dose. [source] Anatoxin-a toxin in the cyanobacterium Planktothrix rubescens from a fishing pond in northern ItalyENVIRONMENTAL TOXICOLOGY, Issue 3 2004Emanuela Viaggiu Abstract A heavy algal bloom occurring in a fishing pond in northern Italy full of Salmo trutta was examined for algae taxonomy and toxic production. The dominant algal species (98%) was identified as the cyanobacterium Planktothrix rubescens (D.C. ex GOMONT) Komarek Anagnostidis, based on morphological examination, and it was revealed to be toxic in mouse and Vibrio fischeri bioassays. The toxin was identified as anatoxin-a using high-performance liquid chromatography and confirmed using liquid chromatography,mass spectrometry (LC-MS). The mouse bioassay gave signs of poisoning, as previously reported for anatoxin-a. The LC-MS confirmed the presence of an anatoxin-a peak at m/z 166 (M+H+). The content of toxin in the field population was estimated at 12.13 ,g/g of fresh cells. The bloom was sustained by the very high N/P ratio in the water. This is the first report in Italy of an anatoxin-a-producing Planktothrix rubescens population. © 2004 Wiley Periodicals, Inc. Environ Toxicol 19: 191,197, 2004. [source] Haematopoietic progenitor cells from the common marmoset as targets of gene transduction by retroviral and adenoviral vectorsEUROPEAN JOURNAL OF HAEMATOLOGY, Issue 4 2001Hitoshi Hibino Abstract: To establish a new non-human primate model for human cytokine and gene therapy, we characterized lymphocytes and haematopoietic progenitor cells of the small New World monkey, the common marmoset. We first assessed the reactions of marmoset bone marrow (BM) and peripheral blood (PB) cells to mouse anti-human monoclonal antibodies (mAbs) for the purpose of isolating marmoset lymphocytes and haematopoietic progenitor cells. Both cell fractions stained with CD4 and CD8 mAbs were identified as lymphocytes by cell proliferation assay and morphological examination. Myeloid-specific mAbs such as CD14 and CD33 did not react with marmoset BM and PB cells. No available CD34 and c-kit mAbs could be used to purify the marmoset haematopoietic progenitor cells. Furthermore, we studied the in vitro transduction of the bacterial ,-galactosidase (LacZ) gene into CFU-GM derived from marmoset BM using retroviral and adenoviral vectors. The transduction efficiency was increased by using a mixed culture system consisting of marmoset BM stromal cells and retroviral producer cells. It was also possible to transduce LacZ gene into marmoset haematopoietic progenitor cells with adenoviral vectors as well as retroviral vectors. The percentage of adenovirally transduced LacZ-positive clusters was 15% at day 4 (multiplicity of infection=200), but only 1,2% at day 14. The differential use of viral vector systems is to be recommended in targeting different diseases. Our results suggested that marmoset BM progenitor cells were available to examine the transduction efficiency of various viral vectors in vitro. [source] Using the Acetabulum to Estimate Age at Death of Adult Males,JOURNAL OF FORENSIC SCIENCES, Issue 2 2006Carme Rissech Ph.D. ABSTRACT: The acetabular region is often present and adequately preserved in adult human skeletal remains. Close morphological examination of the 242 left male os coxae from the identified collection of Coimbra (Portugal) has enabled the recognition of seven variables that can be used to estimate age at death. This paper describes these variables and argues their appropriateness by analyzing the correlation between these criteria and the age, the intra- and interobserver consistence, and the accuracy in age prediction using Bayesian inference to estimate age of identified specimens. Results show significant close correlation between the acetabular criteria and age, nonsignificant differences in intra- and interobserver test, and 89% accuracy in Bayes prediction. Obtained estimated age of the specimens had similar accuracy in all ages. These results indicate that these seven variables, based on the acetabular area, are potentially useful to estimate age at death for adult specimens. [source] Winged presoldiers induced by a juvenile hormone analog in Zootermopsis nevadensis: Implications for plasticity and evolution of caste differentiation in termitesJOURNAL OF MORPHOLOGY, Issue 1 2003Toru Miura Abstract To elucidate the switching mechanism of caste differentiation in termites and to examine the possible induction of soldier-reproductive intercastes experimentally, we investigated the effects of juvenile hormone on the morphologies of soldier caste by applying a juvenile hormone analog (JHA) to nymphs of the damp-wood termite Zootermopsis nevadensis (Isoptera : Termopsidae). JHA treatment for about 2 weeks induced a variety of intermediate castes, showing both alate and soldier morphological features. The principal component analysis (PCA) of those morphological characters showed that those intercastes were a deviation from the developmental line into alates to soldier differentiation, which is known to be triggered by juvenile hormone. Detailed morphological examination of the compound eyes, wing joint, and mandibles showed that those intercastes expressed soldier features, although they had started to develop alate characteristics. The morphology of the resultant intercastes seemed to be determined by the nymphal stage, at which JHA treatment was applied. The induced intercastes with exaggerated soldier-specific characteristics (e.g., mandibles) repressed alate-specific characteristics (e.g., wings), namely, the alate and soldier morphological characteristics in induced intercastes show opposite responses against the application of JHA. On the other hand, ovarian development was not suppressed by the JHA application, even in the soldier-like individuals. Naturally differentiated presoldiers also possessed developed ovarioles, although ovaries of mature soldiers were degenerated. Our results suggest that the juvenile hormone plays complicated roles in the expression of caste morphologies and ovarian development in termites. J. Morphol. 257:22,32, 2003. © 2003 Wiley-Liss, Inc. [source] The use of random amplified polymorphic DNA (RAPD) markers to identify strawberry varieties: a forensic applicationMOLECULAR ECOLOGY, Issue 2 2000L. Congiu Abstract The random amplified polymorphic DNA (RAPD) technique was applied to settle a lawsuit involving unauthorized commercialization of a patented strawberry variety of high economical relevance (,Marmolada'®). Because of economical involvements, the molecular approach was added to the more traditional morphological examination in a double-blind test. All plants belonging to the patented variety were unambiguously identified (13 plants among a total of 31 plants examined). The results were accepted as evidence in the court. This study confirms that the RAPD technique is especially suitable for identification of asexually reproduced plant varieties for forensic or agricultural purposes. [source] The 1,1000 ,m spectral energy distributions of far-infrared galaxiesMONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY, Issue 2 2006A. Sajina ABSTRACT Galaxies selected at 170 ,m by the Infrared Space Observatory (ISO) Far-IR BACKground (FIRBACK) survey represent the brightest ,10 per cent of the cosmic infrared background. Examining their nature in detail is therefore crucial for constraining models of galaxy evolution. Here, we combine Spitzer archival data with previous near-infrared (near-IR), far-IR, and submillimetre (submm) observations of a representative sample of 22 FIRBACK galaxies spanning three orders of magnitude in IR luminosity. We fit a flexible, multicomponent, empirical SED model of star-forming galaxies designed to model the entire ,1,1000 ,m wavelength range. The fits are performed with a Markov Chain Monte Carlo (MCMC) approach, allowing for meaningful uncertainties to be derived. This approach also highlights degeneracies such as between Td and ,, which we discuss in detail. From these fits and standard relations we derive: LIR, LPAH, star formation rate (SFR), ,V, M*, Mdust, Td, and ,. We look at a variety of correlations between these and combinations thereof in order to examine the physical nature of these galaxies. Our conclusions are supplemented by morphological examination of the sources, and comparison with local samples. We find the bulk of our sample to be consistent with fairly standard size and mass disc galaxies with somewhat enhanced star formation relative to local spirals, but likely not bona fide starbursts. A few higher- z luminous infrared galaxies (LIGs) and ultraluminous infrared galaxies (ULIGs) are also present, but contrary to expectation, they are weak mid-IR emitters and overall are consistent with star formation over an extended cold region rather than concentrated in the nuclear regions. We discuss the implications of this study for understanding populations detected at other wavelengths, such as the bright 850-,m Submillimetre Common-User Bolometer Array (SCUBA) sources or the faint Spitzer 24-,m sources. [source] External urethral sphincter activity in diabetic ratsNEUROUROLOGY AND URODYNAMICS, Issue 5 2008Guiming Liu Abstract Aim To examine the temporal effects of diabetes on the bladder and the external urethral sphincter (EUS) activity in rats. Methods Female Sprague-Dawley rats (n,=,24) were divided into two groups: streptozotocin-induced diabetic rats and age-matched controls. Cystometrograms (CMGs) were taken under urethane anesthesia and electromyograms (EMG) of the EUS were evaluated in all rats at 6 and 20 weeks after diabetes induction. After EMG assessment, the tissues of the urethra were harvested for morphological examination. Results Diabetes caused reduction of body weight, but an increase in bladder weight. CMG measurements showed diabetes increased threshold volume, contraction duration, high-frequency oscillations (HFO), and residual volume. Peak contraction amplitude increased in 6-week but not 20-week diabetic rats. EUS-EMG measurements showed increased frequency of EUS-EMG bursting discharge during voiding in 6-week diabetic rats (8.1,±,0.2 vs. 6.9,±,0.6/sec) but not in 20-week (5.8,±,0.3 vs. 6.0,±,0.2/sec) diabetic rats compared with controls. EUS-EMG bursting periods were also increased in both 6-week and 20-week diabetic rats compared with controls. EUS-EMG silent periods were reduced in 6-week diabetic rats, but were not changed in 20-week diabetic rats compared with controls. Active periods did not change in 20-week diabetic rats, but increased in 6-week diabetic rats compared with controls. Morphometric analysis showed atrophy of the EUS after 20 week but not 6 weeks of DM induction. Conclusions Our data indicates diabetes causes functional and anatomical abnormalities of the EUS. These abnormalities may contribute to the time-dependent bladder dysfunction in diabetic rats. Neurourol. Urodynam. 27:429,434, 2008. © 2008 Wiley-Liss, Inc. [source] Resurrection of the genus Selleophytum (Asteraceae: Coreopsideae)NORDIC JOURNAL OF BOTANY, Issue 2 2004Mesfin Tadesse After thorough morphological examination, a consideration of chromosome numbers and molecular studies, the genus Selleophytum is resurrected. A description of the only known species in the genus with an illustration is provided A neotype is selected. [source] Unique histological characteristics of Scedosporium that could aid in its identificationPATHOLOGY INTERNATIONAL, Issue 2 2010Masatomo Kimura Scedosporium prolificans has been increasingly recognized as an etiological agent of disseminated mycelial infections in profoundly immunocompromised patients. Reported herein is a case of disseminated S. prolificans infection in a patient undergoing anti-neoplastic chemotherapy for acute myeloid leukemia. Antemortem blood culture yielded S. prolificans, which was confirmed on conventional morphological examination and polymerase chain reaction-based DNA sequencing targeting internally transcribed spacer regions. Histopathology of autopsy specimens indicated fungal infection in the heart, lungs, liver, kidneys, spleen, pancreas and gastrointestinal tract, with the development of hemorrhagic and ischemic necrosis. The infecting fungus had developing septate hyphae and was identified as belonging to the genus Scedosporium, on in situ hybridization of tissue. The combination of haphazardly branching hyphae and lemon-shaped conidia appeared to be the most useful distinguishing features to allow differentiation of this fungus from other filamentous fungi in tissue. Three other unique histopathological characteristics of the fungus were noted: (i) parallel hyphae bridged at right angles to produce letter-H patterns; (ii) intravascular conidiation; and (iii) purple conidia in tissue, though these are usually described as brown in most text books. Precise histopathology, in addition to other techniques such as in situ hybridization, can aid in the identification of etiological fungi. [source] A Morphological Study of Age Changes in Adult Human Auricular Cartilage With Special Emphasis on Elastic FibersTHE LARYNGOSCOPE, Issue 5 2001Isamu Ito MD Abstract Objective It is well known that the size of the human auricle increases after it has finished development. The reason why the size of the human auricle continues to enlarge until advanced age after reaching adulthood was investigated by observation of the ultrastructure of elastic fibers in human auricular cartilage. Methods A total of 1958 subjects (966 males and 992 females) were classified into 18 age groups from 0 to 5 years up to 85 years and above by 5-year intervals. Ear length, ear width, and length of ear attachment were measured with calipers. Human auricular cartilage was obtained from 26 subjects (16 males and 10 females) aged 14 to 79 years, stained by orcein, and examined by light and electron microscopy. Results Each item of measurement of human auricular size increased significantly with age in both males and females. On morphological examination by light and electron microscopy after orcein staining, elastic fibers in the cartilage were almost homogeneous in diameter and surrounded the cartilage lacuna in bundle-like fashion in young persons, whereas those in elderly persons were heterogeneous in thickness and had many fragments surrounding the territorial matrix. In elderly persons, collagen-like fibers and small vesicles with heterogeneous electron density were frequently observed near elastic bundles around the territorial matrix. Conclusion Structural changes of auricular cartilage associated with morphological age changes of elastic fibers may be one of the causes of expansion of the auricle after reaching adulthood. [source] Effect of , -Interferon and , -Tocopherol in Reversing Hepatic Cirrhosis in RatsANATOMIA, HISTOLOGIA, EMBRYOLOGIA, Issue 2 2007T. C. Mathew Summary The aim of this study was to assess the effects of , -interferon and , -tocopherol (vitamin E), or a combination of both, in reversing hepatic fibrosis following the induction of cirrhosis using thioacetamide by histological and biochemical analysis. Fifty male Wistar rats were used in this study. The animals were divided equally into five groups. Animals in group I were used as controls. The remaining animals (groups II,V) were provided with 0.5 g/L of thioacetamide in order to induce liver cirrhosis. Group II animals were used as the cirrhotic control. Animals of groups III, IV and V were given , -interferon, , -tocopherol and interferon together with , -tocopherol, respectively, for 30 days. After 30 days the animals were killed and following gross morphological examination of the liver, the hepatic tissues were processed for histological analysis and the serum was used for liver function tests. Morphological analysis showed a decrease in the number of nodules on the surface of the liver in both interferon- as well as vitamin E-treated cirrhotic rats. Histopathological analysis showed that the abnormalities of the cirrhotic liver were partially reversed and liver function tests showed an overall improvement following treatment of animals of groups III, IV and V. Combination therapy using both interferon and , -tocopherol did not have any substantial effect on the rats compared with that when they were given separately. These findings suggest that , -interferon and , -tocopherol may have therapeutic value in reversing liver cirrhosis. [source] Computerized analysis of cytochemical reactions for dehydrogenases and oxygraphic studies as methods to evaluate the function of the mitochondrial sheath in rat spermatozoaANDROLOGIA, Issue 1 2001M. Piasecka Cytochemical reactions for mitochondrial NADH-dependent dehydrogenases (diaphorase/NADH which is related to flavoprotein), NAD-dependent dehydrogenases (isocitrate, malate) and succinate dehydrogenase were carried out in rat spermatozoa. In addition to a morphological evaluation, the intensity of the reactions was assessed using a computer image analysing system (Quantimet 600 S). The intensity of the reactions was examined in sperm midpieces by measuring integrated optical density (IOD) and mean optical density (MOD). The activity of mitochondrial respiratory chain complexes was also analysed using the polarographic method. In the population of spermatozoa studied, all whole spermatozoa midpieces were completely filled with formazans, the product of the cytochemical reaction. These morphological findings corresponded to the values obtained for IOD and MOD for the given enzymes. In the oxygraphic studies, the spermatozoa demonstrated consumption of oxygen in the presence of substrates for I, II and IV complexes and their mitochondria revealed normal integrity and sensitivity to the substrates and inhibitors. However, the oxygraphic studies revealed differences between the sperm and somatic cells. These differences concerned the stimulation of pyruvate oxidation by malate, the lack of an effect of malonic acid on phenazine methosulphate (an acceptor of electrons) oxidation and the lack of an effect of cytochrome c on ascorbate oxidation. The cytochemical method, together with densitometric measurements, enables: (1) the reaction intensity to be determined objectively; (2) subtle and dramatic differences in reaction intensity to be revealed between spermatozoa that do not differ under morphological evaluation of the intensity; (3) possible defects within the mitochondrial sheath to be located and assessed in a large number of spermatozoa. This method can be used as a screening method alongside the routine morphological examination of spermatozoa. On the other hand, the oxygraphic method in the inner membrane of mitochondria can reveal functional changes which are related to the action of respiratory chain complexes and display characteristic features of mitochondria energy metabolism. The methods used are complementary and allow the complex evaluation of mitochondria in spermatozoa. Both methods can be used in experimental and clinical studies. [source] Preterm and at term children: morphological and functional analysis of optic nerve and visual pathway with OCT, HRT and pVEPACTA OPHTHALMOLOGICA, Issue 2009R ANGELI Purpose To compare optic nerve morphology and visual function in 14 preterm children(mean gestational age:33,38) with normal cerebral ultrasound at birth and 15 at term children (mean gestational age:39,67). Methods Mean preterm age was 7,6 years, mean at term was 9,1 years. Children underwent to complete functional and morphological evaluation of Optic Nerve Head (ONH) with Heidelberg Retinal Tomography and Optical Coherence Tomography. The same children were evaluated at birth and at the time of morphological examinations by mean of transient and steady state pVEP. Results Mean visual acuity was 9,85 in the preterm, 10 in the at term children. HRT in preterm and at term children at term(n=28) preterm(n=25) P value mean sd mean sd Disc Area 2,5 0,7 2,34 0,68 0,38 Cup Area 0,50 0,44 0,58 0,47 0,51 Rim Area 2 0,45 1,76 0,37 0,047 C/D Area Ratio 0,18 0,12 0,22 0,14 0,23 C/D Linear Ratio 0,4 0,15 0,45 0,16 0,30 Mean RNFL Thick 0,2 0,07 0,24 0,2 0,26 Tab.1 A statistically significant reduction was found in preterm children as regard rim area (p=0,047,tab 1) at HRT and superior RNFL thickness (p=0,01), temporal and inferior inner macular thickness (p=0,03) at OCT. Differences in the pVep latencies were found at 3 and 8 months after birth, but not at the time of morphological examination. Steady State pVEP amplitudes differences were not statistically significant. Conclusion Morphologic optic disk and macula differences between term and preterm children seems not functionally affect the visual pathway. [source] Fusarium incarnatum isolated from black tiger shrimp, Penaeus monodon Fabricius, with black gill disease cultured in VietnamJOURNAL OF FISH DISEASES, Issue 9 2004L V Khoa Abstract Fusarium incarnatum was isolated from gill lesions of cultured black tiger shrimp, Penaeus monodon, in every crop during 2000,2002 in Nghe An province, Vietnam. Infected shrimps showed typical signs of black gill disease and mortalities about a month prior to harvest. Detailed morphological examinations, as well as molecular phylogenic analyses based on partial nucleotide sequences of ribosomal DNA, were made on the isolates. An artificial infection of kuruma prawn, Penaeus japonicus, using two selected isolates was also conducted and their pathogenicity determined. [source] Preterm and at term children: morphological and functional analysis of optic nerve and visual pathway with OCT, HRT and pVEPACTA OPHTHALMOLOGICA, Issue 2009R ANGELI Purpose To compare optic nerve morphology and visual function in 14 preterm children(mean gestational age:33,38) with normal cerebral ultrasound at birth and 15 at term children (mean gestational age:39,67). Methods Mean preterm age was 7,6 years, mean at term was 9,1 years. Children underwent to complete functional and morphological evaluation of Optic Nerve Head (ONH) with Heidelberg Retinal Tomography and Optical Coherence Tomography. The same children were evaluated at birth and at the time of morphological examinations by mean of transient and steady state pVEP. Results Mean visual acuity was 9,85 in the preterm, 10 in the at term children. HRT in preterm and at term children at term(n=28) preterm(n=25) P value mean sd mean sd Disc Area 2,5 0,7 2,34 0,68 0,38 Cup Area 0,50 0,44 0,58 0,47 0,51 Rim Area 2 0,45 1,76 0,37 0,047 C/D Area Ratio 0,18 0,12 0,22 0,14 0,23 C/D Linear Ratio 0,4 0,15 0,45 0,16 0,30 Mean RNFL Thick 0,2 0,07 0,24 0,2 0,26 Tab.1 A statistically significant reduction was found in preterm children as regard rim area (p=0,047,tab 1) at HRT and superior RNFL thickness (p=0,01), temporal and inferior inner macular thickness (p=0,03) at OCT. Differences in the pVep latencies were found at 3 and 8 months after birth, but not at the time of morphological examination. Steady State pVEP amplitudes differences were not statistically significant. Conclusion Morphologic optic disk and macula differences between term and preterm children seems not functionally affect the visual pathway. [source] |