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Modest Changes (modest + change)
Kinds of Modest Changes Selected AbstractsAcute changes in carbon dioxide levels alter the electroencephalogram without affecting cognitive functionPSYCHOPHYSIOLOGY, Issue 4 2000Elisabeth Bloch-Salisbury The partial pressure of carbon dioxide in the arterial blood (PaCO2) is usually tightly regulated, yet it varies among healthy people at rest (range ,32,44 mmHg) as well as within an individual during many natural life situations. The present study examined whether modest changes in end-tidal PCO2 (PetCO2; a noninvasive measure of PaCO2) affect electroencephalographic (EEG) activity, cognitive function, and vigilance. Nine adults were ventilated mechanically using a mouthpiece; respiratory rate and breath size were held constant while PetCO2 was set to levels that produced minimal discomfort. Despite discrete changes in EEG, neither acute PetCO2 increases (mean = 47 mmHg) nor decreases (mean = 30 mmHg) from resting levels (mean = 38 mmHg) affected performance on cognitive tasks, latency or amplitude of the N1, P2, or P3 event-related potential, or alertness. Modest changes in PetCO2 may cause significant alterations in the EEG without disturbing cognitive function. [source] More aspiration than achievement?HEALTH & SOCIAL CARE IN THE COMMUNITY, Issue 6 2006Children's complaints, advocacy in health services in Wales Abstract The present paper reports on key results from a government-funded survey of all National Health Service trusts, local health boards and community health councils in Wales, which was conducted in 2004,2005 to identify the characteristics of complaints involving children, and the use of professional advocacy services in these complaints and their role in supporting children in relation to health service matters more generally. Findings from the survey are presented which reveal the marginal take-up of professional advocacy services in health complaints, and the slender resource in professional advocacy for children commissioned by a small number of health bodies. Advocacy support for users of health services typically focuses upon adult-related issues. The needs of children, particularly those who may have special requirements because of disability, being looked after, or having language or cultural needs are not well met according to the present survey. This raises the question of whether recent policy and guidance on advocacy (particularly for children and vulnerable groups) is seen by health bodies as warranting decisive action and dedicated investment, or whether rhetoric and modest change is the more likely outcome in the face of other pressing demands on health budgets. [source] Causes of variability in the summertime Antarctic boundary-layer climateINTERNATIONAL JOURNAL OF CLIMATOLOGY, Issue 13 2007Dirk van As Abstract A high-resolution one-dimensional atmospheric model is used to assess the contribution of various surface characteristics and external forcings on the structure and dynamics of the atmospheric boundary layer (ABL) over the Antarctic Plateau in summer. The reference run simulates the boundary layer over a mildly sloping surface (1.5 m km,1) for a clear sky near the end of the Antarctic summer (31 January-3 February). The ABL depth is approximately 100 m. At night, a low-level jet forms due to the combined effect of katabatic forcing and an inertial oscillation. During the day a convective mixed layer is present. As expected, the ABL is very sensitive to surface slope; a larger slope forces higher wind speeds and a deeper boundary layer. Over a horizontal surface, a nocturnal jet is also found as a result of the inertial oscillation. A modest change in surface albedo alters the mixed-layer temperature and the height and strength of the nocturnal jet considerably. Rotating the large-scale wind relative to the slope direction also has a large impact on ABL depth and structure. The deepest boundary layer and largest wind speed over a northward down-sloping surface are found for an easterly (cross slope) large-scale wind, as is typical for Antarctica. A very shallow ABL with low wind speed is found for the opposite large-scale wind direction. ABL sensitivity to surface roughness was found to be small. For all experiments, the ABL sensitivity is enhanced due to the positive feedback between the cooling of the ABL and katabatic wind speed. Copyright © 2007 Royal Meteorological Society [source] Seasonal influenza activity in Hong Kong and its association with meteorological variationsJOURNAL OF MEDICAL VIROLOGY, Issue 10 2009Paul K.S. Chan Abstract Influenza seasons appear consistently in the temperate regions, but are more variable in tropical/subtropical regions. The determinant for such variation remains poorly understood. This study documented the activity of influenza over a 10-year period in Hong Kong; examining its association with changes in temperature and relative humidity. The two types of influenza exhibited different correlations with meteorological variations. Influenza A showed two seasonal peaks occurring respectively in winter/spring and summer months in most years. Influenza B showed a clear winter/spring peak, but its activity during summer months was more variable. Cold and humid conditions were associated with a higher activity of both influenza A and B. In contrast, hot and humid conditions were associated with a higher activity of influenza A, but were associated with only a moderate, less consistent increase in the activity of influenza B. A trend of increase in the magnitude of summer peaks of influenza A, but not influenza B, was observed. A hypothetical 2°C rise in temperature would decrease the proportion of favorable days for influenza A in December,April from 78% to 57%, but an increase from 58% to 71% in May,November; with a similar effect (from 83% to 62%) for influenza B during December,April, but a modest change (from 17% to 18%) during May,November. The presence of two seasonal peaks of influenza annually emphasizes the need to evaluate the duration of protective immunity offered by vaccination. Further study on the effects of climate change and global warming on the activity of influenza is warranted. J. Med. Virol. 81:1797,1806, 2009. © 2009 Wiley-Liss, Inc. [source] Copper binding to octarepeat peptides of the prion protein monitored by mass spectrometryPROTEIN SCIENCE, Issue 2 2000Randy M. Whittal Abstract Electrospray ionization mass spectrometry (ESI-MS) was used to measure the binding of Cu2+ ions to synthetic peptides corresponding to sections of the sequence of the mature prion protein (PrP). ESI-MS demonstrates that Cu2+ is unique among divalent metal ions in binding to PrP and defines the location of the major Cu2+ binding site as the octarepeat region in the N-terminal domain, containing multiple copies of the repeat ProHisGlyGlyGlyTrpGlyGln. The stoichiometries of the complexes measured directly by ESI-MS are pH dependent: a peptide containing four octarepeats chelates two Cu2+ ions at pH 6 but four at pH 7.4. At the higher pH, the binding of multiple Cu2+ ions occurs with a high degree of cooperativity for peptides C-terminally extended to incorporate a fifth histidine. Dissociation constants for each Cu2+ ion binding to the octarepeat peptides, reported here for the first time, are mostly in the low micromolar range; for the addition of the third and fourth Cu2+ ions to the extended peptides at pH 7.4, KD's are <100 nm. n-terminal acetylation of the peptides caused some reduction in the stoichiometry of binding at both ph's. cu2+ also binds to a peptide corresponding to the extreme N-terminus of PrP that precedes the octarepeats, arguing that this region of the sequence may also make a contribution to the Cu2+ complexation. Although the structure of the four-octarepeat peptide is not affected by pH changes in the absence of Cu2+, as judged by circular dichroism, Cu2+ binding induces a modest change at pH 6 and a major structural perturbation at pH 7.4. It is possible that PrP functions as a Cu2+ transporter by binding Cu2+ ions from the extracellular medium under physiologic conditions and then releasing some or all of this metal upon exposure to acidic pH in endosomes or secondary lysosomes. [source] Modulation of rabbit sinoatrial node activation sequence by acetylcholine and isoproterenol investigated with optical mapping techniqueACTA PHYSIOLOGICA, Issue 4 2009D. V. Abramochkin Abstract Aims:, Changes in the rabbit sinoatrial node (SAN) activation sequence with the cholinergic and adrenergic factors were studied. The correlation between the sinus rhythm rate and the leading pacemaker site shift was determined. The hypothesis concerning the cholinergic suppression of nodal cell excitability as one of the mechanisms associated with pacemaker shift was tested. Methods:, A high-resolution optical mapping technique was used to register beat-to-beat changes in the SAN activation pattern under the influence of the cholinergic and adrenergic factors. Results:, Acetylcholine (10 ,m) and strong intramural parasympathetic nerve stimulation caused a pacemaker shift as well as rhythmic slowing and the formation of an inexcitable region in the central part of SAN. In this region the generation of action potentials was suppressed. The slowing of the sinus rhythm (which exceeded 12.8 ± 3.1% of the rhythm control rate) always accompanied the pacemaker shift. Isoproterenol (10, 100 nm, 1 ,m) and sympathetic postganglionic nerve stimulation also evoked a pacemaker shift but without formation of an inexcitable zone. The acceleration of the sinus rhythm, which exceeded 10.5 ± 1.3% of the control rate of the rhythm, always accompanied the shift. Conclusions:, Both cholinergic and adrenergic factors cause pacemaker shifts in the rabbit SAN. While modest changes in the sinus rhythm do not coincide with the pacemaker shift, greater changes always accompany the shift and may be caused by it, according to one hypothesis. The formation of an inexcitable zone at the place where the leading pacemaker is situated is one of the mechanisms associated with pacemaker shift. [source] Retinoic acid induces CDK inhibitors and growth arrest specific (Gas) genes in neural crest cellsDEVELOPMENT GROWTH & DIFFERENTIATION, Issue 3 2005Linping Wang Retinoic acid (RA), the active metabolite of vitamin A, regulates cellular growth and differentiation during embryonic development. In excess, this vitamin is also highly teratogenic to animals and humans. The neural crest is particularly sensitive to RA, and high levels adversely affect migration, proliferation and cell death. We investigated potential gene targets of RA associated with neural crest proliferation by determining RA-mediated changes in gene expression over time, using microarrays. Statistical analysis of the top ranked RA-regulated genes identified modest changes in multiple genes previously associated with cell cycle control and proliferation including the cyclin-dependent kinase inhibitors Cdkn1a (p21), Cdkn2b (p15INK4b), and Gas3/PMP22. The expression of p21 and p15INK4b contribute to decreased proliferation by blocking cell cycle progression at G1-S. This checkpoint is pivotal to decisions regulating proliferation, apoptosis, or differentiation. We have also confirmed the overexpression of Gas3/PMP22 in RA-treated neural crests, which is associated with cytoskeletal changes and increased apoptosis. Our results suggest that increases in multiple components of diverse regulatory pathways have an overall cumulative effect on cellular decisions. This heterogeneity contributes to the pleiotropic effects of RA, specifically those affecting proliferation and cell death. [source] Impact of perfluorooctanoic acid on fathead minnow (Pimephales promelas) fatty acyl-coa oxidase activity, circulating steroids, and reproduction in outdoor microcosmsENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 8 2004Ken D. Oakes Abstract This study investigates reproductive impairment and biochemical changes in fathead minnow (Pimephales promelas) exposed for 39 d to varying concentrations of perfluorooctanoic acid (PFOA) under microcosm conditions. While the concentrations tested in this study were much higher than those normally found in the environment, no mortality was associated with PFOA exposure. Only modest changes were observed in condition factor and in relative liver and gonad size. Significant declines in circulating plasma steroids were observed, but these were accompanied by only limited increases in time to first oviposition and decreases in overall egg production. Peroxisome proliferation, as quantified by fatty acyl-CoA oxidase (FAO) activity, was elevated with low PFOA concentrations but attenuated with exposure to higher PFOA doses. Little evidence was seen of differential induction of peroxisome-associated enzyme activity with sex. Oxidative stress, as quantified by the 2-thiobarbituric acid reactive substances (TBARS) assay, was only modestly influenced by PFOA exposure and is not a significant consequence of FAO activity in fathead minnow. Perfluorooctanoic acid appears to be relatively nontoxic at environmentally relevant concentrations but may impact biochemical and reproductive endpoints under conditions associated with environmental spills. [source] Cardiac sympathetic nerve activity during kainic acid,induced limbic cortical seizures in ratsEPILEPSIA, Issue 4 2009Harumi Hotta Summary We sought to define changes in cardiac sympathetic nerve activity that occur during seizures. We studied kainic acid,induced limbic cortical seizures in urethane-anesthetized rats using cardiac sympathetic nerve, blood pressure, and electrocardiography (ECG) recordings. We studied changes in ventilation rate before and during seizures. Cardiac sympathetic nerve activity was increased during limbic cortical seizures. The modest increases were similar to changes induced by nitroprusside infusion. The normal relation of cardiac sympathetic nerve activity to ventilation rate was lost during seizure activity. Changes in cardiac sympathetic nerve activity caused by changes in ventilation rate became unpredictable, and could be extreme. We conclude that the modest changes in cardiac sympathetic nerve activity contribute to the predominantly parasympathetic effects on the heart during limbic cortical seizures and periods of asphyxia. Further, ventilation rate changes might be associated with large sudden increases or decreases in cardiac sympathetic outflow during seizures. [source] Dynamics of action potential backpropagation in basal dendrites of prefrontal cortical pyramidal neuronsEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 4 2008Wen-Liang Zhou Abstract Basal dendrites of neocortical pyramidal neurons are relatively short and directly attached to the cell body. This allows electrical signals arising in basal dendrites to strongly influence the neuronal output. Likewise, somatic action potentials (APs) should readily propagate back into the basilar dendritic tree to influence synaptic plasticity. Two recent studies, however, determined that sodium APs are severely attenuated in basal dendrites of cortical pyramidal cells, so that they completely fail in distal dendritic segments. Here we used the latest improvements in the voltage-sensitive dye imaging technique (Zhou et al., 2007) to study AP backpropagation in basal dendrites of layer 5 pyramidal neurons of the rat prefrontal cortex. With a signal-to-noise ratio of >,15 and minimal temporal averaging (only four sweeps) we were able to sample AP waveforms from the very last segments of individual dendritic branches (dendritic tips). We found that in short- (< 150 µm) and medium (150,200 µm in length)-range basal dendrites APs backpropagated with modest changes in AP half-width or AP rise-time. The lack of substantial changes in AP shape and dynamics of rise is inconsistent with the AP-failure model. The lack of substantial amplitude boosting of the third AP in the high-frequency burst also suggests that in short- and medium-range basal dendrites backpropagating APs were not severely attenuated. Our results show that the AP-failure concept does not apply in all basal dendrites of the rat prefrontal cortex. The majority of synaptic contacts in the basilar dendritic tree actually received significant AP-associated electrical and calcium transients. [source] The refolding of type II shikimate kinase from Erwinia chrysanthemi after denaturation in ureaFEBS JOURNAL, Issue 8 2002Eleonora Cerasoli Shikimate kinase was chosen as a convenient representative example of the subclass of ,/, proteins with which to examine the mechanism of protein folding. In this paper we report on the refolding of the enzyme after denaturation in urea. As shown by the changes in secondary and tertiary structure monitored by far UV circular dichroism (CD) and fluorescence, respectively, the enzyme was fully unfolded in 4 m urea. From an analysis of the unfolding curve in terms of the two-state model, the stability of the folded state could be estimated as 17 kJ·mol,1. Approximately 95% of the enzyme activity could be recovered on dilution of the urea from 4 to 0.36 m. The results of spectroscopic studies indicated that refolding occurred in at least four kinetic phases, the slowest of which (k = 0.009 s,1) corresponded with the regain of shikimate binding and of enzyme activity. The two most rapid phases were associated with a substantial increase in the binding of 8-anilino-1-naphthalenesulfonic acid with only modest changes in the far UV CD, indicating that a collapsed intermediate with only partial native secondary structure was formed rapidly. The relevance of the results to the folding of other ,/, domain proteins is discussed. [source] On the climate and weather of mountain and sub-arctic lakes in Europe and their susceptibility to future climate changeFRESHWATER BIOLOGY, Issue 12 2009R. THOMPSON Summary 1.,The complex terrain and heterogeneous nature of the mountain environment coupled with remoteness from major centres of human activity makes mountains challenging locations for meteorological investigations. Mountainous areas tend to have more varied and more extreme weather than lowlands. 2.,The EMERGE program has the primary aim of assessing the status of remote mountain and sub-arctic lakes throughout Europe for the first time. In this study, we describe the main features of the climate, ice-cover durations and recent temperature trends of these areas. The main weather characteristics of European mountain and sub-arctic lakes are their cold temperatures and year-round precipitation. Mean annual temperatures are generally close to 0 °C, and maximum summer temperatures reasonably close to 10 °C. 3.,Maritime versus continental settings determine the main differences in annual-temperature range among lake districts (10.5 °C in Scotland to 26.7 °C in Northern Finland), and a similar factor for ice-cover duration. Radiation ranges from low (120 W m,2) in the high latitude sub-arctic and high (237 W m,2) in the southern ranges of the Pyrenees and Rila. Similarly, precipitation is high in the main Alpine chain (250 cm year,1 in the Central Southern Alps) and low in the continental sub-arctic (65 cm year,1 in Northern Finland). 4.,The main temporal patterns in air temperature follow those of the adjacent lowlands. All the lake districts warmed during the last century. Spring temperature trends were highest in Finland; summer trends were weak everywhere; autumn trends were strongest in the west, in the Pyrenees and western Alps; while winter trends varied markedly, being high in the Pyrenees and Alps, low in Scotland and Norway and negative in Finland. 5.,Two new, limnological case studies on Lake Redon, in the Pyrenees, highlight the sensitivity of remote lakes to projected changes in the global climate. These two case studies involve close linkages between extreme chemical-precipitation events and synoptic wind-patterns, and between thermocline behaviour and features of the large-scale circulation. 6.,Individual lakes can be ultra-responsive to climate change. Even modest changes in future air temperatures will lead to major changes in lake temperatures and ice-cover duration and hence probably affect their ecological status. [source] Ionic Iridium(III) Complexes with Bulky Side Groups for Use in Light Emitting Cells: Reduction of Concentration QuenchingADVANCED FUNCTIONAL MATERIALS, Issue 13 2009Carsten Rothe Abstract Here, the photophysics and performance of single-layer light emitting cells (LECs) based on a series of ionic cyclometalated Ir(III) complexes of formulae and where ppy, bpy, and phen are 2-phenylpyridine, substituted bipyridine and substituted phenanthroline ligands, respectively, are reported. Substitution at the N,N ligand has little effect on the emitting metal-ligand to ligand charge-transfer (MLLCT) states and functionalization at this site of the complex leads to only modest changes in emission color. For the more bulky complexes the increase in intermolecular separation leads to reduced exciton migration, which in turn, by suppressing concentration quenching, significantly increases the lifetime of the excited state. On the other hand, the larger intermolecular separation induced by bulky ligands reduces the charge carrier mobility of the materials, which means that higher bias fields are needed to drive the diodes. A brightness of ca. 1000,cd,m,2 at 3,V is obtained for complex 5, which demonstrates a beneficial effect of bulky substituents. [source] Overexpression of GSTA2 protects against cell cycle arrest and apoptosis induced by the DNA inter-strand crosslinking nitrogen mustard, mechlorethamineJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 2 2005Jingping Xie Abstract The effectiveness of bifunctional alkylating nitrogen mustard compounds in chemotherapy is related to their ability to form DNA inter-strand crosslinks. Patients exposed to DNA inter-strand crosslinking (ICL) agents subsequently experience an elevated incidence of myelodysplastic syndromes (MDS) and MDS related acute myeloid leukemia. Fanconi's anemia (FA) patients are deficient in the repair of crosslink DNA damage and they experience a high incidence of MDS. These observations indicate that hematopoietic cells are specific target for the transforming effects of DNA crosslinking damage. Changes in transcript levels were characterized in human hematopoietic cells occurring in response to the nitrogen mustard, mechlorethamine (HN2), but not in response to monofunctional analogs. Only modest changes in a few gene transcripts were detected in HL60 cells exposed to levels of HN2 tittered to maximal dose that caused growth suppression with minimal cell death and allowed eventual resumption of normal cell growth. Under conditions of transient growth suppression, a subset of glutathione-S-transferase (GST) isoenzyme genes was consistently upregulated three to fourfold by HN2, but not by monofunctional analogs. Subsequent efforts to confirm the changes detected by microarray analyses revealed an unexpected dependence on treatment conditions. The GST alpha class A2 subfamily member transcripts were upregulated 24 h after a 1 h exposure to HN2 that caused an extensive, but transient block in late S/G2 cell cycle phase, but were minimally altered with continuous exposure. The 1-h exposure to HN2 caused a transient late S/G2 cell cycle arrest in both the HL-60 cell line and the Colo 320HSR human colon cancer cell line. Overexpression of GSTA2 by transient transfection protected Colo 320HSR cells against both cycle arrest and apoptosis following exposure to HN2. Overexpression of GSTA2 in Colo 320HSR cells induced after exposure to HN2 did not alter cycle arrest or apoptosis. The results indicate that human GSTA2 facilitates the protection of cells from HN2 damage and not repair. Our results are consistent with the possibility that GSTA2 polymorphisms, variable isoenzyme expression, and variable induced expression may be factors in the pathogenesis of MDS. © 2005 Wiley-Liss, Inc. [source] Affective and Adrenocorticotrophic Responses to Photoperiod in Wistar RatsJOURNAL OF NEUROENDOCRINOLOGY, Issue 2 2008B. J. Prendergast The present study tested the hypothesis that seasonal intervals of exposure to modest changes in photoperiod, typical of those experienced by humans living in temperate latitudes (10,14 h light/day), engage changes in emotional behaviour of Wistar rats, a commonly-used animal model for investigations of affective physiology. Short day lengths (, 12 h light/day) induced behavioural despair in a forced-swim test, exploratory anxiety in an open field arena, and anhedonia in a two-bottle sucrose preference task, relative to longer day lengths. Plasma adrenocorticotrophic hormone was lower in short-day relative to long-day rats, but testosterone and corticosterone concentrations were comparable across treatments. In common with animals that engage reproductive responses to day length, reproductively nonresponsive mammals such as Wistar rats exhibit changes in affective state following small changes in day length. Wistar rats may provide an animal model for the study of seasonal mood regulation because the neuroendocrine, depressive, anxious and anhedonic responses of Wistar rats to short days bear similarities to those observed in some human populations. Standard laboratory husbandry practices (exposure to a 12 : 12 h light/dark cycle) may inadvertently deliver a chronic background depressive and anxiogenic stimulus. [source] Ocular phenotype in a mouse gene knockout model for infantile neuronal ceroid lipofuscinosisJOURNAL OF NEUROSCIENCE RESEARCH, Issue 5 2006Bo Lei Abstract Mutations in the human protein palmitoyl thioesterase-1 (PPT-1) gene result in an autosomal recessive neurodegenerative disorder designated neuronal ceroid lipofuscinosis (NCL), type CLN1, or infantile NCL. Among the symptoms of the CLN1 disease are accumulation of autofluorescent lysosomal storage bodies in neurons and other cell types, seizures, motor and cognitive decline, blindness, and premature death. Development of an effective therapy for this disorder will be greatly assisted by the availability of suitable animal models. A mouse PPT-1 gene knockout model has recently been generated. Studies were performed to determine whether the mouse model exhibits ocular features of the human CLN1 disorder. A progressive accumulation of autofluorescent storage material in all layers of the retina was observed in the PPT-1 knockout mice. Accompanying the storage body accumulation was a modest loss of cells with nuclei in the outer and inner nuclear layers. As indicated by electroretinogram (ERG) responses, retinal function was only mildly impaired at 4 months of age but was severely impaired by 8 months, despite only modest changes in retinal morphology. The pupillary light reflex (PLR), on the other hand, was exaggerated in the knockout mice. The apparent anomaly between the ERG and the PLR findings suggests that disease-related PLR changes may be due to changes in extraocular signal processing. The pronounced ocular phenotype in the PPT-1 knockout mice makes these animals a good model for testing therapeutic interventions for treatment of the human CLN1 disorder. © 2006 Wiley-Liss, Inc. [source] Fluorocarbon End-functionalized Polymers from Poly(arylether) Dendritic InitiatorsMACROMOLECULAR SYMPOSIA, Issue 1 2005A. Pillay Narrainen Abstract Fréchet-type poly(arylether) first and second generation (G1 and G2 respectively) dendrons were prepared from 1-(bromomethyl)-3,5-bis(trifluoromethyl)benzene. The latter and the brominated versions of the two dendrons were successfully employed in the copper mediated living radical polymerization (LRP) of styrene-d8 giving polymers of predictable molecular weights and narrow polydispersities. Contact angle measurements and ion beam analysis were used to explore the adsorption of these materials to the air-polymer surface in blended films with unfunctionalized hydrogenous polystyrene. Although contact angle analysis indicated only modest changes in the hydrophobicity and lipophobicity of the surface, ion beam analysis clearly showed the formation of an excess layer of dendron functionalized polymer at the exposed surface that increased with increasing fluorine content. [source] Reflectance spectra of iron meteorites: Implications for spectral identification of their parent bodiesMETEORITICS & PLANETARY SCIENCE, Issue 2 2010Edward A. CLOUTIS Powder spectra are invariably red-sloped over this wavelength interval and have a narrow range of visible albedos (approximately 10,15% at 0.56 ,m). Metal (Fe:Ni) compositional variations have no systematic effect on the powder spectra, increasing grain size results in more red-sloped spectra, and changes in viewing geometry have variable effects on overall reflectance and spectral slope. Roughened metal slab spectra have a wider, and higher, range of visible albedos than powders (22,74% at 0.56 ,m), and are also red-sloped. Smoother slabs exhibit greater differences from iron meteorite powder spectra, exhibiting wider variations in overall reflectance, spectral slopes, and spectral shapes. No unique spectral parameters exist that allow for powder and slab spectra to be fully separated in all cases. Spectral differences between slabs and powders can be used to constrain possible surface properties, and causes of rotational spectral variations, of M-asteroids. The magnitude of spectral variations between M-asteroids and rotational and spectral variability does not necessarily imply a dramatic change in surface properties, as the differences in albedo and/or spectral slope can be accommodated by modest changes in grain size (for powders), small changes in surface roughness (for slabs), or variations in viewing geometry. Since metal powders exhibit much less spectral variability than slabs, M-asteroid spectral variability requires larger changes in either powder properties or viewing geometry than for slabs for a given degree of spectral variation. [source] Differential expression of three members of the AMT1 gene family encoding putative high-affinity NH4+ transporters in roots of Oryza sativa subspecies indicaPLANT CELL & ENVIRONMENT, Issue 6 2003A. KUMAR ABSTRACT In order to investigate the molecular basis of high-affinity ammonium absorption by roots of rice plants (Oryza sativa subspecies indica) the expression patterns of three members of the AMT1 family of genes in rice seedling roots in response to altered nitrogen provision and diurnal changes in irradiance were examined. The 13NH4+ influx and transcript levels of OsAMT1.1 in roots decreased several fold within 48 h when plants acclimated to 10 µm external NH4+ for 3 weeks were transferred to 10 mm NH4+. Likewise when plants acclimated in 10 mm NH4+ were transferred to 10 µm NH4+, there was an equally rapid up-regulation of OsAMT1.1 and 13NH4+ influx in the roots. Changes in transcript abundance of OsAMT1.2 following these treatments were approximately 50% less than in OsAMT1.1, and changes of OsAMT1.3 expression were even less. By contrast, in response to the diurnal changes of irradiance, root transcript abundance of OsAMT1.3 and 15NH4+ influx increased approximately three-fold late in the photoperiod, whereas OsAMT1.1 and OsAMT1.2 exhibited only modest changes. The present results suggest that high-affinity NH4+ influx is differentially regulated at the transcriptional level through the expression of three members of the OsAMT1 family of genes in roots of rice seedlings in response to changes of N status and daily irradiance. In general, these findings are in agreement with earlier observations in Arabidopsis and tomato. [source] Acute changes in carbon dioxide levels alter the electroencephalogram without affecting cognitive functionPSYCHOPHYSIOLOGY, Issue 4 2000Elisabeth Bloch-Salisbury The partial pressure of carbon dioxide in the arterial blood (PaCO2) is usually tightly regulated, yet it varies among healthy people at rest (range ,32,44 mmHg) as well as within an individual during many natural life situations. The present study examined whether modest changes in end-tidal PCO2 (PetCO2; a noninvasive measure of PaCO2) affect electroencephalographic (EEG) activity, cognitive function, and vigilance. Nine adults were ventilated mechanically using a mouthpiece; respiratory rate and breath size were held constant while PetCO2 was set to levels that produced minimal discomfort. Despite discrete changes in EEG, neither acute PetCO2 increases (mean = 47 mmHg) nor decreases (mean = 30 mmHg) from resting levels (mean = 38 mmHg) affected performance on cognitive tasks, latency or amplitude of the N1, P2, or P3 event-related potential, or alertness. Modest changes in PetCO2 may cause significant alterations in the EEG without disturbing cognitive function. [source] Effects of Ureaplasma urealyticum infection on the male reproductive system in experimental ratsANDROLOGIA, Issue 5 2010Y. Wang Summary To study the effects of Ureaplasma urealyticum (Uu) infection on the male reproductive system, the mechanism of infertility induced by Uu infection was investigated in experimental rats. Male Sprague,Dowley rats were infected with Uu4 (serotype 4) through repeated natural sexual intercourse for 8 weeks to establish infection. After 8 weeks, the blood samples of the animals were collected and analysed for cytokine production, and the animals were microdissected for the analysis of the reproductive system. Morphological study showed that spermatozoa exhibited curling and breaks in the rats infected at different dosages. Of the infected rats, 27.5% had both soft and hard calculi in the urinary tract, compared with 12% in the control groups. Uu infection resulted in a decline of sperm quality, eventually leading to the death of the spermatozoa. In the infected animals, the serum interleukin 6 and interleukin 8 levels increased significantly (P < 0.05), while tumour necrosis factor-alpha and interferon-gamma showed only modest changes. Our observations showed that Uu infection has an impact on sperm morphology, leading to the death of the spermatozoa. It is plausible that the morphological alterations of spermatozoa induced by Uu infection are one of the possible factors that contribute to male infertility. [source] Climate change impacts on investment in crop sowing machinery,AUSTRALIAN JOURNAL OF AGRICULTURAL & RESOURCE ECONOMICS, Issue 2 2009Ross Kingwell A model of investment in crop sowing machinery is applied to wheat production under current and projected climatic conditions at several locations in south-western Australia. The model includes yield responses to time of sowing at each location given current and projected climatic conditions. These yield relationships are based on wheat growth simulation modelling that in turn draws on data from a down-scaled global circulation model. Wheat price distributions and cost of production data at each location, in combination with the time of sowing yield relationships are used to determine a farmer's optimal investment in crop sowing work rate under each climate regime. The key finding is that the impacts of climate change on profit distributions are often marked, yet mostly modest changes in investment in work rate form part of the profit-maximising response to climate change. The investment response at high versus low rainfall locations mostly involves increases and decreases in work rates, respectively. However, changes to investment in work rate within a broadly similar rainfall region are not always uniform. The impacts of climate change on investments in work rate at a particular location are shown to require knowledge of several factors, especially how climate change alters the pattern of yield response to the time of sowing at that location. [source] | |||||||||||||||||||