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Selected AbstractsFine-needle aspiration cytology of Rosai-Dorfman disease of boneDIAGNOSTIC CYTOPATHOLOGY, Issue 7 2008Xin Jing M.D. Abstract Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease) is a rare, benign self-limiting condition of unknown etiology. Less than a quarter of cases have only extranodal involvement and a few cases of skeletal involvement of Rosai-Dorfman disease without associated lymphadenopathy have been reported in the literature. We herein report cytohistologic findings in a case of sole skeletal Rosai-Dorfman disease in a 51-year-old woman who presented with an expansile, heterogeneous lesion at T11 with cord compression and edema. A CT-guided fine-needle aspiration of T-11 lesion was performed and the sample was processed by ThinPrep technique. The ThinPrep smear showed characteristic features of Rosai-Dorfman disease including hypercellularity with moderate number of histiocytes in a background of lymphocytes, plasma cells, and neutrophils. The histiocytes possessed abundant, pale and vacuolated cytoplasm, rounded nuclei with smooth nuclear membranes, fine chromatin, and distinct nucleoli. The histiocytes showed emperipolesis of lymphocytes and neutrophils. The diagnosis was confirmed by concurrent biopsy with immunhistochemical study. Our case highlighted the role of fine-needle aspiration with ThinPrep technique in the diagnosis of Rosai-Dorfman disease. Diagn. Cytopathol. 2008;36:516,518. © 2008 Wiley-Liss, Inc. [source] Photomutagenicity of thiabendazole, a postharvest fungicide, in bacterial assaysENVIRONMENTAL AND MOLECULAR MUTAGENESIS, Issue 2 2003Mie Watanabe-Akanuma Abstract We investigated the photomutagenicity of thiabendazole (TBZ), a postharvest fungicide commonly used on imported citrus fruits. Using UVA light (320,400 nm), we irradiated bacterial cultures with or without TBZ in a 24-well multiplate. UVA-irradiation without TBZ was not mutagenic to the tester strains, nor was unirradiated TBZ. TBZ was strongly photomutagenic in Escherichia coli WP2uvrA and WP2uvrA/pKM101 strains, weakly photomutagenic in Salmonella typhimurium TA100 and TA98, and not photomutagenic in S. typhimurium TA1535 and TA1538. The photomutagenicity of TBZ was more evident in WP2uvrA/pKM101, which carries the trpE65 ochre mutation (TAA), than in TA100, which carries the hisG46 missense mutation (CCC). In E. coli WP3101,WP3106 and the corresponding pKM101-containing strains, photoactivated TBZ induced predominantly G:C,A:T transitions and A:T,T:A transversions. In the plasmid-containing strains only, TBZ induced a moderate number of A:T,G:C transitions and a few A:T,C:G and G:C,T:A transversions. The observation that UVA-irradiated TBZ mutated both G:C and A:T basepairs may explain why WP2uvrA/pKM101 was more sensitive to its mutagenicity than TA100. TBZ that was irradiated before it was added to the WP2uvrA/pKM101 cells was not photomutagenic, which suggests that the photomutagenic products of TBZ were unstable or rapidly reacted with other molecules before being incorporated into cells. Environ. Mol. Mutagen. 41:92,98, 2003. © 2003 Wiley-Liss, Inc. [source] Linkage analysis with sequential imputationGENETIC EPIDEMIOLOGY, Issue 1 2003Zachary Skrivanek Abstract Multilocus calculations, using all available information on all pedigree members, are important for linkage analysis. Exact calculation methods in linkage analysis are limited in either the number of loci or the number of pedigree members they can handle. In this article, we propose a Monte Carlo method for linkage analysis based on sequential imputation. Unlike exact methods, sequential imputation can handle large pedigrees with a moderate number of loci in its current implementation. This Monte Carlo method is an application of importance sampling, in which we sequentially impute ordered genotypes locus by locus, and then impute inheritance vectors conditioned on these genotypes. The resulting inheritance vectors, together with the importance sampling weights, are used to derive a consistent estimator of any linkage statistic of interest. The linkage statistic can be parametric or nonparametric; we focus on nonparametric linkage statistics. We demonstrate that accurate estimates can be achieved within a reasonable computing time. A simulation study illustrates the potential gain in power using our method for multilocus linkage analysis with large pedigrees. We simulated data at six markers under three models. We analyzed them using both sequential imputation and GENEHUNTER. GENEHUNTER had to drop between 38,54% of pedigree members, whereas our method was able to use all pedigree members. The power gains of using all pedigree members were substantial under 2 of the 3 models. We implemented sequential imputation for multilocus linkage analysis in a user-friendly software package called SIMPLE. Genet Epidemiol 25:25,35, 2003. © 2003 Wiley-Liss, Inc. [source] A forward-only recursion algorithm for MAP decoding of linear block codesINTERNATIONAL JOURNAL OF ADAPTIVE CONTROL AND SIGNAL PROCESSING, Issue 8 2002Hans-Jürgen Zepernick Abstract The evolution of digital mobile communications along with the increase of integrated circuit complexity has resulted in frequent use of error control coding to protect information against transmission errors. Soft decision decoding offers better error performance compared to hard decision decoding but on the expense of decoding complexity. The maximum a posteriori (MAP) decoder is a decoding algorithm which processes soft information and aims at minimizing bit error probability. In this paper, a matrix approach is presented which analytically describes MAP decoding of linear block codes in an original domain and a corresponding spectral domain. The trellis-based decoding approach belongs to the class of forward-only recursion algorithms. It is applicable to high rate block codes with a moderate number of parity bits and allows a simple implementation in the spectral domain in terms of storage requirements and computational complexity. Especially, the required storage space can be significantly reduced compared to conventional BCJR-based decoding algorithms. Copyright © 2002 John Wiley & Sons, Ltd. [source] A new parallel algorithm of MP2 energy calculationsJOURNAL OF COMPUTATIONAL CHEMISTRY, Issue 4 2006Kazuya Ishimura Abstract A new parallel algorithm has been developed for second-order Møller,Plesset perturbation theory (MP2) energy calculations. Its main projected applications are for large molecules, for instance, for the calculation of dispersion interaction. Tests on a moderate number of processors (2,16) show that the program has high CPU and parallel efficiency. Timings are presented for two relatively large molecules, taxol (C47H51NO14) and luciferin (C11H8N2O3S2), the former with the 6-31G* and 6-311G** basis sets (1032 and 1484 basis functions, 164 correlated orbitals), and the latter with the aug-cc-pVDZ and aug-cc-pVTZ basis sets (530 and 1198 basis functions, 46 correlated orbitals). An MP2 energy calculation on C130H10 (1970 basis functions, 265 correlated orbitals) completed in less than 2 h on 128 processors. © 2006 Wiley Periodicals, Inc. J Comput Chem 27: 407,413, 2006 [source] Proteomic analysis of human vessels: Application to atherosclerotic plaquesPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 6 2003Mari Carmen Duran Abstract Atherosclerosis is a chronic disease that affects medium and large arteries. This process originates from the interaction between cells of the arterial wall, lipoproteins and inflammatory cells, leading to the development of complex lesions or plaques that protrude into the arterial lumen. Plaque rupture and thrombosis result in acute clinical complications such as myocardial infarction and stroke. Owing to the heterogeneous cellular composition of the plaques, a proteomic analysis of the whole lesion is not appropriate. Therefore, we have studied the proteins secreted by human carotid atherosclerotic plaques, obtained by endarterectomy. Normal artery segments and different regions of the surgical pieces (noncomplicated plaque, complicated plaque with thrombus) were cultured in protein-free medium and the secreted proteins (supernatants) analyzed by two-dimensional gel electrophoresis. Normal artery segments secreted a moderate number of proteins (42 spots). However in the two-dimensional (2-D) gels (pH 3,10) of segments bearing a plaque, the number of spots increased markedly (154). The number of spots also increased (202) in the 2-D gels of artery segments with a ruptured plaque and thrombus. Thus, the more complicated the lesion, the higher the number of secreted proteins, suggesting the production of specific proteins relating to the complexity of the atherosclerotic lesion. [source] The Degree of Desulphurization of a Limestone/Gypsum Wet FGD Spray Tower using Response Surface MethodologyCHEMICAL ENGINEERING & TECHNOLOGY (CET), Issue 4 2007Z. Zhao Abstract The degree of desulphurization was studied using response surface methodology (RSM), which enables effect examinations of parameters with a moderate number of experiments. All experiments were conducted in a lab-scale spray tower for limestone/gypsum wet flue gas desulphurization (FGD). The model flue gas was prepared from air and SO2 gas. The SO2 concentrations in the gas phase were determined by a multi-method analyzer. The degree of desulphurization correlated well with operating parameters, including pH, L/G, T, and v, with a determination coefficient R,Sq of 0.964. Effect tests indicate that L/G has the most significant influence on the degree of desulphurization. The interactions of L/G with pH, and with v, both play important roles. The result indicates that the evolutive response surface model is helpful to describe the degree of desulphurization of the limestone/gypsum wet FGD spray tower. [source] | |||||||||||||