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Selected AbstractsCharacteristics of microcystin production in the cell cycle of Microcystis viridisENVIRONMENTAL TOXICOLOGY, Issue 1 2004Keishi Kameyama Abstract The correlation between the content of three microcystins (types LR, RR and YR) and the cell cycle of an axenic strain of Microcystis viridis, NIES-102, was investigated under conditions of high (16 mg L,1) and low (1.0 mg L,1) nitrate (NO3 -N) concentrations. Each phase of the cell cycle was identified using a flow cytometer equipped with a 488-nm argon laser using SYTOX Green dye, which binds specifically to nucleic acids and can be exited by the wavelength (Ex/Em: 504/523 nm on DNA). Microcystin concentration showed a positive linear correlation with DNA concentration. The microcystin content of the cells changed remarkably as the cell cycle process proceeded, with maximum content in the G2/M phase and minimum content in the G0/G1 phase. Under a condition of high NO3 -N concentration, the ratio of the total content in the G0/G1 phase to that in the G2/M phase was about 6:1. In contrast, for the two batch cultures the total content was 1.3-fold greater in the G2/M phase. The compositions of the three microcystins also changed along with the cell cycle process, although there was little difference in composition that was related to NO3 -N concentration. Therefore, there were distinctive compositions specific to each phase of the cycle, and the cell cycle of the M. viridis strain was more strongly responsible for both the quantity and the types of microcystin production than was the effect of NO3 -N concentration. © 2004 Wiley Periodicals, Inc. Environ Toxicol 19: 20,25, 2004. [source] Palynological, physico-chemical and colour characterization of Moroccan honeys: I. River red gum (Eucalyptus camaldulensis Dehnh) honeyINTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 4 2003Anass Terrab Summary The melissopalynological, physico-chemical and colour characteristics of Eucalyptus honeys from Morocco were studied. It is generally accepted that a minimum content of 70% of Eucalyptus pollen is necessary to classify an Eucalyptus honey as unifloral. Twenty-eight physico-chemical and five colour parameters were measured, including the content of sugars and minerals, proline, water content, pH, acidity, hydroxymethylfurfural, diastase activity, ash, electrical conductivity, colour coordinates (L*, a*, b*), chroma (C*ab) and hue angle (hab). Pollen analysis was included, and samples contained a mean of six pollen types (range 2,12). The majority of the samples (75%) contained more than 90%Eucalyptus pollen. The presence of Quercus, Plantago and Thymelaea pollen allows the differentiation of these honeys from those with a different geographical origin. Physico-chemical analysis showed that there was a low percentage of sucrose ( = 0.01%) and trisaccharides. Mineral analysis showed a low percentage of Mg ( = 25.99 ppm) and K ( = 205 ppm), while electrical conductivity ( = 721 ,S cm,1) and diastase activity ( = 39.05 °Gothe) showed medium-high values. The colour parameters lightness (L*) and chroma (C*ab) showed relatively low values. [source] SOURCE ROCK PROPERTIES OF LACUSTRINE MUDSTONES AND COALS (OLIGOCENE DONG HO FORMATION), ONSHORE SONG HONG BASIN, NORTHERN VIETNAMJOURNAL OF PETROLEUM GEOLOGY, Issue 1 2005H. I. Petersen Oligocene lacustrine mudstones and coals of the Dong Ho Formation outcropping around Dong Ho, at the northern margin of the mainly offshore Cenozoic Song Hong Basin (northern Vietnam), include highly oil-prone potential source rocks. Mudstone and coal samples were collected and analysed for their content of total organic carbon and total sulphur, and source rock screening data were obtained by Rock-Eval pyrolysis. The organic matter composition in a number of samples was analysed by reflected light microscopy. In addition, two coal samples were subjected to progressive hydrous pyrolysis in order to study their oil generation characteristics, including the compositional evolution in the extracts from the pyrolysed samples. The organic material in the mudstones is mainly composed of fluorescing amorphous organic matter, liptodetrinite and alginite with Botryococcus-morphology (corresponding to Type I kerogen). The mudstones contain up to 19.6 wt.% TOC and Hydrogen Index values range from 436,572 mg HC/g TOC. From a pyrolysis S2 versus TOC plot it is estimated that about 55% of the mudstones'TOC can be pyrolised into hydrocarbons; the plot also suggests that a minimum content of only 0.5 wt.% TOC is required to saturate the source rock to the expulsion threshold. Humic coals and coaly mudstones have Hydrogen Index values of 318,409 mg HC/g TOC. They are dominated by huminite (Type III kerogen) and generally contain a significant proportion of terrestrial-derived liptodetrinite. Upon artificial maturation by hydrous pyrolysis, the coals generate significant quantities of saturated hydrocarbons, which are probably expelled at or before a maturity corresponding to a vitrinite reflectance of 0.97%R0. This is earlier than previously indicated from Dong Ho Formation coals with a lower source potential. The composition of a newly discovered oil (well B10-STB-1x) at the NE margin of the Song Hong Basin is consistent with contributions from both source rocks, and is encouraging for the prospectivity of offshore half-grabens in the Song Hong Basin. [source] What's at Stake in Natural Law?NEW BLACKFRIARS, Issue 1023 2008Barrister, David McIlroy M.A. (Cantab.), Mtr Dt (Toulouse) Abstract Something like natural law is required if Christians are to say that Jesus Christ is as relevant to human beings of every age and in every place that we have ever existed as a race. There must be something stable about the human condition which means that we are all alike in need of a Saviour. That something is the fact that we are created to love God and to love our neighbour. This much is revealed to all humankind. For the Apostle Paul and Thomas Aquinas the natural law was not given as an alternative method of salvation but rather to explain the justice of God's judgment and the utter gratuity of divine grace. Similarly, natural theology is not an assertion that faith in Christ is optional but rather that all human beings are culpable if they do not recognise that there is a god who created them and rewards those who seek God. Natural theology is the minimum content of faith where Christ has not been proclaimed; it is no substitute for explicit faith in Christ when He has been revealed. [source] Integration of magnetism and heavy metal chemistry of soils to quantify the environmental pollution in Kathmandu, NepalISLAND ARC, Issue 4 2005Pitambar Gautam Abstract Soil profiles of the Kathmandu urban area exhibit significant variations in magnetic susceptibility (,) and saturation isothermal remanence (SIRM), which can be used to discriminate environmental pollution. Magnetic susceptibility can be used to delineate soil intervals by depth into normal (< 10,7 m3/kg), moderately enhanced (10,7,< 10,6 m3/kg) and highly enhanced (, 10,6 m3/kg). Soils far from roads and industrial sites commonly fall into the ,normal' category. Close to a road corridor, soils at depths of several centimeters have the highest ,, which remains high within the upper 20 cm interval, and decreases with depth through ,moderately magnetic' to ,normal' at approximately 30,40 cm. Soils in the upper parts of profiles in urban recreational parks have moderate ,. Soil SIRM has three components of distinct median acquisition fields (B1/2): soft (30,50 mT, magnetite-like phase), intermediate (120,180 mT, probably maghemite or soft coercivity hematite) and hard (550,600 mT, hematite). Close to the daylight surface, SIRM is dominated by a soft component, implying that urban pollution results in enrichment by a magnetite-like phase. Atomic absorption spectrometry of soils from several profiles for heavy metals reveals remarkable variability (ratio of maximum to minimum contents) of Cu (16.3), Zn (14.8) and Pb (9.3). At Rani Pokhari, several metals are well correlated with ,, as shown by a linear relationship between the logarithmic values. At Ratna Park, however, both , and SIRM show significant positive correlation with Zn, Pb and Cu, but poor and even negative correlation with Fe (Mn), Cr, Ni and Co. Such differences result from a variety of geogenic, pedogenic, biogenic and man-made factors, which vary in time and space. Nevertheless, for soil profiles affected by pollution (basically traffic-related), , exhibits a significant linear relationship with a pollution index based on the contents of some urban elements (Cu, Pb, Zn), and therefore it serves as an effective parameter for quantifying the urban pollution. [source] | ||||||||||