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Miniaturization
Selected AbstractsCover Picture: Electrophoresis 24'2009ELECTROPHORESIS, Issue 24 2009Article first published online: 10 DEC 200 Issue no. 24 has 20 contributions on "Miniaturization". This special issue of Miniaturization "features reports on the latest developments in miniaturized systems focused on Instrumentation, Bioanalysis, and Column Technology and Microfluidic Protocols". In addition, the issue presents a Fast Track article describing a microwell array device with integrated microfluidic components for enhanced single-cell analysis. [source] Cover Picture: Electrophoresis 14'09ELECTROPHORESIS, Issue 14 2009Article first published online: 28 JUL 200 Issue no. 14 is an Emphasis Issue with 9 articles on various aspects of "Proteins and Proteomics" while the remaining 14 articles are arranged into 4 different parts including "Microfluidics and Miniaturization", "Genotyping and Transcriptomics", "Enantioseparations", and "Nanoparticles and Abused Drugs Analyses". Selected articles are: Effective elimination of nucleic acids from bacterial protein samples for optimized blue native polyacrylamide gel electrophoresis ((10.1002/elps.200900026)) 2-D difference in gel electrophoresis combined with Pro-Q Diamond staining: A successful approach for the identification of kinase/phosphatase targets ((10.1002/elps.200800780)) Microvalves actuated sandwich immunoassay on an integrated microfluidic system ((10.1002/elps.200800818)) Chemical gradient-mediated melting curve analysis for genotyping of SNPs ((10.1002/elps.200800729)) [source] Cover Picture: Electrophoresis 9'09ELECTROPHORESIS, Issue 9 2009Article first published online: 7 MAY 200 Issue no. 9 is an Emphasis Issue with 7 articles on various aspects of "Microfluidics and Miniaturization" while the remaining articles are grouped into sections on "Detection Sensitivity Enhancement and Stacking", "Binding Studies" and "Other Methodologies". In addition, issue no. 9 has two Fast Track articles. The first on proteome alteration of early-stage differentiation of mouse embryonic stem cells into hepatocyte-like cells, and the second on dielectrophoretic separation of small particles in a sawtooth channel. [source] Cover Picture: Electrophoresis 14/2008ELECTROPHORESIS, Issue 14 2008Article first published online: 23 JUL 200 Issue 14 is a regular issue including an Emphasis Section offering a series of 9 papers on ,Microfluidics and Miniaturization". These 9 research papers report on various topics including studying single DNA molecules, selective release of intracellular molecules on the single cell level, isoelectric focusing of proteins in an ordered micropillar array, sample stream focusing in a microchip, integrated microfluidic system for sensing infectious viral disease, EOF in annulus and rectangular channels, confinement effects on monolith morphology, accumulation and filtering of nanoparticles in microchannels, and carbon nanotubes disposable detectors. [source] Cover Picture: Electrophoresis 6/2008ELECTROPHORESIS, Issue 6 2008Article first published online: 18 MAR 200 Regular issues provide a wide range of research and review articles covering all aspects of electrophoresis. Here you will find cutting-edge articles on methods and theory, instrumentation, nucleic acids, CE and CEC, miniaturization and microfluidics, proteomics and two-dimensional electrophoresis. In addition, issue no. 6 features a series of 7 important papers on "Microfluidics and Miniaturization" dealing with LCD-based optoelectronic tweezers, cell sorting, single cell clone analysis and cultivation, integrated ITP stacking and gel electrophoresis, floating injection, electrokinetic flows on thermosensitive surfaces and flow velocity measurement in CE chip instruments. [source] Miniaturization of Fluorescence Microscopes Using Fibre OpticsEXPERIMENTAL PHYSIOLOGY, Issue 6 2002Fritjof Helmchen In both medical research and diagnostics characterization of biological tissue on the cellular level relies on high-resolution optical microscopy. In most cases, however, tissue is excised for microscopic investigation, in part because conventional microscopes are bulky instruments. Imaging of cells in the intact living organism has been difficult. Over the last decade several groups have developed miniature confocal microscopes that use fibre optics to deliver light to the specimen and to measure either reflected or excited fluorescence light. In addition, two-photon excitation recently has been employed in a small portable ,fibrescope'. A potential clinical application of these microscope probes is their endoscopic use for optical biopsy of inner organs or guidance of conventional biopsy. As a mobile research tool they may permit imaging of neuronal activity in the brain of awake, behaving animals. Here, we review technological approaches to build miniaturized fluorescence microscopes and discuss their potential applications. [source] Photoluminescence-Based Sensing With Porous Silicon Films, Microparticles, and NanoparticlesADVANCED FUNCTIONAL MATERIALS, Issue 20 2009Michael J. Sailor Abstract Here, chemical sensors made from porous Si are reviewed, with an emphasis on systems that harness photoluminescence and related energy- and charge-transfer mechanisms available to porous Si-derived nanocrystallites. Quenching of luminescence by molecular adsorbates involves the harvesting of energy from a delocalized nanostructure that can be much larger than the molecule being sensed, providing a means to amplify the sensory event. The interaction of chemical species on the surface of porous Si can exert a pronounced influence on this process, and examples of some of the key chemical reactions that modify either the surface or the bulk properties of porous Si are presented. Sensors based on micron-scale and smaller porous Si particles are also discussed. Miniaturization to this size regime enables new applications, including imaging of cancerous tissues, indirect detection of reactive oxygen species (ROS), and controlled drug release. Examples of environmental and in vivo sensing systems enabled by porous Si are provided. [source] Miniaturization of a Laser Doppler Blood Flow Sensor by System-in-Package Technology: Fusion of an Optical Microelectromechanical Systems Chip and Integrated CircuitsIEEJ TRANSACTIONS ON ELECTRICAL AND ELECTRONIC ENGINEERING, Issue 2 2010Wataru Iwasaki Member Abstract We have developed the first and the smallest blood flow sensor composed of integrated circuits (ICs) fused with an optical microelectromechanical systems (MEMS) chip using system-in-package (SiP) technologies for application in a healthcare monitoring system. The probe of this blood flow sensor consists of three layers, and the optical MEMS chip is stacked as the top layer. Through silicon via (TSV), vertical-cavity surface-emitting laser (VCSEL) and cavities enable wafer-level packaging of the optical MEMS chip. The other two layers consisting of ICs are highly densified by SiP technology, and the volume of the probe is miniaturized to about one-sixth of our previously reported integrated laser Doppler blood flowmeter, an MEMS blood flow sensor to which SiP technology was not applied. Copyright © 2010 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc. [source] Miniaturized rectangular waveguide filtersINTERNATIONAL JOURNAL OF RF AND MICROWAVE COMPUTER-AIDED ENGINEERING, Issue 4 2007Alexander Shelkovnikov Abstract Two types of miniaturized rectangular waveguide filters are presented. Miniaturization is achieved using the slow-wave effect of electromagnetic bandgap (EBG) surfaces and the left-handed properties of split ring resonators (SRRs). The proposed EBG waveguide bandpass filter performs passband in the frequency range, which corresponds to the waveguide with the lower recommended operating band consequently enabling significant miniaturization of the structure. The SRR-loaded bandstop filter makes use of the effect imposed by left-handed medium (LHM), which is created by a combination of SRRs and wireline on the dielectric slab. Both filters are designed, simulated, and tested. Experimental results of the SRR-loaded bandstop filter are presented to demonstrate feasibility of the proposed structures. © 2007 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2007. [source] A Forensic Laboratory Tests the Berkeley Microfabricated Capillary Array Electrophoresis Device,JOURNAL OF FORENSIC SCIENCES, Issue 4 2008Susan A. Greenspoon Ph.D. Abstract:, Miniaturization of capillary electrophoresis onto a microchip for forensic short tandem repeat analysis is the initial step in the process of producing a fully integrated and automated analysis system. A prototype of the Berkeley microfabricated capillary array electrophoresis device was installed at the Virginia Department of Forensic Science for testing. Instrument performance was verified by PowerPlex® 16 System profiling of single source, sensitivity series, mixture, and casework samples. Mock sexual assault samples were successfully analyzed using the PowerPlex® Y System. Resolution was assessed using the TH01, CSF1PO, TPOX, and Amelogenin loci and demonstrated to be comparable with commercial systems along with the instrument precision. Successful replacement of the Hjerten capillary coating method with a dynamic coating polymer was performed. The accurate and rapid typing of forensic samples demonstrates the successful technology transfer of this device into a practitioner laboratory and its potential for advancing high-throughput forensic typing. [source] Miniaturized elliptic bandpass filter using the novel coplanar double stepped impedance resonatorsMICROWAVE AND OPTICAL TECHNOLOGY LETTERS, Issue 6 2006Suk-Hyung Jang Abstract This paper presents the design and experimental results of the new miniaturized elliptic coplanar bandpass filter (EC BPF) using two types of double stepped impedance resonators (DSIRs) operating at a center frequency of 1.38 GHz. Miniaturization in the proposed BPF is achieved by connecting the transmission line between the middle of two SIRs contained in a single DSIR and both grounded planes. In addition, bond-wire bridges connected between both grounded planes (which are needed in various coplanar waveguide structures) are not required. The new four-pole EC-DSIR BPF shows good agreement with the simulation data obtained by full-wave EM simulations using Zeland IE3D ver. 10.06. © 2006 Wiley Periodicals, Inc. Microwave Opt Technol Lett 48: 1059,1063, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.21600 [source] Fe3+ immobilized metal affinity chromatography with silica monolithic capillary column for phosphoproteome analysisPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 3 2007Shun Feng Abstract Immobilized metal affinity chromatography (IMAC) is a commonly used technique for phosphoproteome analysis due to its high affinity for adsorption of phosphopeptides. Miniaturization of IMAC column is essential for the analysis of a small amount of sample. Nanoscale IMAC column was prepared by chemical modification of silica monolith with iminodiacetic acid (IDA) followed by the immobilization of Fe3+ ion inside the capillary. It was demonstrated that Fe3+ -IDA silica monolithic IMAC capillary column could specifically capture the phosphopeptides from tryptic digest of ,-casein with analysis by MALDI-TOF MS. The silica monolithic IMAC capillary column was manually coupled with nanoflow RPLC/nanospray ESI mass spectrometer (,RPLC,nanoESI MS) for phosphoproteome analysis. The system was validated by analysis of standard phosphoproteins and then it was applied to the analysis of protein phosphorylation in mouse liver lysate. Besides MS/MS spectra, MS/MS/MS spectra were also collected for neutral loss peak. After database search and manual validation with conservative criteria, 29 singly phosphorylated peptides were identified by analyzing a tryptic digest of only 12,,g mouse liver lysate. The results demonstrated that the silica monolithic IMAC capillary column coupled with ,RPLC-nanoESI MS was very suitable for the phosphoproteome analysis of minute sample. [source] Development of a Novel Membrane Aerated Hollow-Fiber MicrobioreactorBIOTECHNOLOGY PROGRESS, Issue 2 2008Louis Villain A new challenge in biotechnological processes is the development of flexible bioprocessing platforms, allowing strain selection, facilitating scale-up and integrating separation steps. Miniaturization of such a cultivation system allows parallel use and the saving of resources but makes the supply of oxygen to the cells difficult. In this work we present a membrane aerated hollow-fiber microbioreactor (HFMBR) which consists of an acrylic glass module equipped with two different types of membrane fibers. Fibers of polyethersulfone and polyvinyldifluoride were used for substrate and oxygen supply, respectively. Cultivation of E. coli as model organism and production of His-tagged GFP were carried out in the extracapillary space of the membrane aerated HFMBR and compared with cultivations in shaking flask which are commonly used for screening experiments. The measurement of the oxygen transfer capacity and the online monitoring of the dissolved oxygen during the cultivation were performed using a fiber optic oxygen sensor. Online measurement of the optical density was also integrated to the bioreactor. Due to efficient oxygen transfer, a better cell growth than in the shaking flask experiments was achieved, while no negative influence on the GFP productivity was observed in the membrane aerated bioreactor. Thus the feasibility of a future integrated downstreaming could also be demonstrated. [source] The lack of significant changes in scalp hair follicle density with advancing ageBRITISH JOURNAL OF DERMATOLOGY, Issue 4 2005R. Sinclair Summary Background, Age-related reduction in hair is seen in the axillary and pubic regions as well as the scalp; however, it has not been investigated qualitatively on the scalp. Horizontally sectioned scalp biopsy is an ideal tool to investigate the impact of advancing age on scalp hair follicle density and morphology. Objectives, To examine the effect of age and follicle miniaturization on total hair count in 1666 horizontally sectioned mid-scalp biopsies from 928 women aged between 13 and 84 years with hair loss. Methods Setting:, Specialist hair loss referral clinic in a teaching hospital. Design: Analysis of data set. Methods:, All scalp biopsies were 4 mm in diameter and taken from the crown. Miniaturization was assessed by calculating the ratio of terminal to vellus-like hairs (T/V) at the mid-isthmus level and considered significant if the ratio was , 4 : 1. Fibrosis was documented when present. Linear regression was used to examine the association between total hair count, age and miniaturization. Results, The average number of hair follicles per biopsy was 39·6 (SD ± 10·8). A highly significant negative association (P < 0·0001) was found between age and total follicle number, although the predictive value of age in total hair count was found to be small [root error mean square (R2) < 2%]. Controlling for T/V , 4 : 1, the association was weakened, but remained significant. The relationship unconfounded by T/V , 4 : 1 shows that for every additional year of ageing, 0·077 total hair follicles (0·22%) are lost per biopsy. Conclusions, Age and follicular miniaturization were found to be extremely weak predictors of total hair count in women with hair loss. [source] Miniaturization of Microwave-Assisted Carbohydrate Functionalization to Create Oligosaccharide MicroarraysCHEMBIOCHEM, Issue 3 2006Matthias A. Brun A microwave-assisted Kochetkov reaction (a) has been used on different reducing carbohydrates to obtain glycosylamines and then N -iminoglycosylamidines without purification. These glycoconjugates were then used to construct microarrays to test known carbohydrate,protein interactions. This method streamlines efforts required to functionalize carbohydrates for construction of arrays. [source] Miniaturization of Powder Dissolution Measurement and Estimation of Particle SizeCHEMISTRY & BIODIVERSITY, Issue 11 2009Alex Avdeef Abstract The objective was to investigate the applicability and limitations of an approach for estimating particle size from powder dissolution measurement using as little as 50,,g of sample in 1,ml of buffer solutions. The powder dissolution profiles of five sparingly-soluble drugs (hydrochlorothiazide, phenazopyridine hydrochloride, 2-naphthoic acid, indomethacin, and dipyridamole) were evaluated with a novel biexponential spherical particle equation and also the Wang,Flanagan spherical particle non-sink equation. The results were compared to particle sizing based on measured specific surface area by the Brunauer,Emmett,Teller (BET) method, and also based on Coulter counting. With the exception of hydrochlorothiazide, the model compounds indicated some agglomeration in the dissolution media. The dry-state specific surface area was larger than expected from either the Coulter method or the powder-dissolution data, especially for phenazopyridine hydrochloride. The particle radii estimated by the powder dissolution method ranged from 10 to 68,,m, with equilibrium solubilities spanning from 5,,g/ml (dipyridamole) to 911,,g/ml (hydrochlorothiazide). Powder dissolution data collected with the miniaturized apparatus can be used to determine particle size, with estimated values agreeing reasonably with those measured by the Coulter counter method. [source] Effects of Finasteride (1 mg) on Hair TransplantDERMATOLOGIC SURGERY, Issue 10 2005Matt Leavitt DO Background. The improved scalp coverage achieved by hair transplant for men with androgenetic alopecia can be diminished by continued miniaturization and loss of preexisting, nontransplanted hairs. Objectives. To evaluate whether finasteride 1 mg, administered daily from 4 weeks before until 48 weeks after hair transplant, improves scalp hair and growth of nontransplanted hair in areas surrounding the transplant and to evaluate the safety and tolerability of finasteride for men undergoing hair transplant. Methods. In this randomized, double-blind, placebo-controlled study, 79 men with androgenetic alopecia (20,45 years of age) were assigned to treatment with finasteride 1 mg (n = 40) or placebo (n = 39) once daily from 4 weeks before until 48 weeks after hair transplant. Efficacy was evaluated by review of global photographs by an expert dermatologist and by macrophotography for scalp hair counts. Results. Treatment with finasteride resulted in significant improvements from baseline, compared with placebo, in scalp hair based on global photographic assessment (p < .01) and hair counts (p < .01) at week 48. Visible increases in superior/frontal scalp hair post-transplant were recorded for 94% and 67% of patients in the finasteride and placebo groups, respectively. Finasteride treatment was generally well tolerated. Conclusion. For men with androgenetic alopecia, therapy with finasteride 1 mg daily from 4 weeks before until 48 weeks after hair transplant improves scalp hair surrounding the hair transplant and increases hair density. [source] The skull of Vanzosaura rubricauda (Squamata: Gymnophthalmidae)ACTA ZOOLOGICA, Issue 4 2009Cecilia Guerra Abstract A detailed description of the cranial osteology and chondrocranium of Vanzosaura rubricauda is made based both on articulated and partially disarticulated material of dry skeletons and cleared and stained specimens, and is compared with other previously studied gymnophthalmids. Some features of Vanzosaura are interesting, such as the reduction of the supratemporal fenestrae, that may reflect a process of miniaturization, and the fusion of the occipital bones in a single oto-occipital complex with no evident sutures. Based on the anatomical evidence, the skull of Vanzosaura is interpreted as amphikinetic. [source] Information Technology and Emergency Medical Care during DisastersACADEMIC EMERGENCY MEDICINE, Issue 11 2004Theodore C. Chan MD Abstract Disaster response to mass-casualty incidents represents one of the greatest challenges to a community's emergency response system. Rescuers, field medical personnel, and regional emergency departments and hospitals must often provide care to large numbers of casualties in a setting of limited resources, inadequate communication, misinformation, damaged infrastructure, and great personal risk. Emergency care providers and incident managers attempt to procure and coordinate resources and personnel, often with inaccurate data regarding the true nature of the incident, needs, and ongoing response. In this chaotic environment, new technologies in communications, the Internet, computer miniaturization, and advanced "smart devices" have the potential to vastly improve the emergency medical response to such mass-casualty incident disasters. In particular, next-generation wireless Internet and geopositioning technologies may have the greatest impact on improving communications, information management, and overall disaster response and emergency medical care. These technologies have applications in terms of enhancing mass-casualty field care, provider safety, field incident command, resource management, informatics support, and regional emergency department and hospital care of disaster victims. [source] A simple method for output voltage control of a three-phase multilevel inverter considering DC voltage fluctuationELECTRICAL ENGINEERING IN JAPAN, Issue 3 2010Kenji Amei Abstract Multilevel inverter circuit generates the stair-like voltage without using transformer and interphase reactor, and it is the circuit which realizes reduction in the harmonics and enlargement of the capacity. In addition, the application of PWM control improves the waveform, and reduces the switching component to the conventional half, and the filter capacity is reduced. In this paper, improvement on the voltage utilization factor and feedback control of output voltage are applied to multilevel inverter circuit. For the DC power supply with the intense fluctuation, it is necessary to construct inverter circuit which can supply stabilized AC voltage. One-chip microcomputer with various functions is used for the control equipment of this circuit, and miniaturization and cost reduction of the control equipment are realized. Here, the control principle and experimental results of this equipment are mainly reported. © 2009 Wiley Periodicals, Inc. Electr Eng Jpn, 170(3): 40,47, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20906 [source] Direct Electrochemical Sensing and Detection of Natural Antioxidants and Antioxidant Capacity in Vitro SystemsELECTROANALYSIS, Issue 22 2007Antonio, Javier Blasco Abstract This review highlights the role of electrochemical approaches in the sensing of antioxidants and their antioxidant capacity with especial attention to the analytical possibilities of electrochemistry in the direct evaluation of antioxidant capacity exhibited by food and biological samples due to the termed dietary, natural or biological antioxidants (mainly polyphenols, and vitamins C and E). The analytical potency of the electrochemistry is comprehensively stated and the selected results found in the literature are summarized and discussed critically. The main electrochemical approaches used have been cyclic voltammetry (CV) and flow injection analysis with amperometric detection (FIA-ED). In addition, miniaturization is going to break new frontiers in the evaluation of antioxidant activity. [source] Label-Free Impedance Biosensors: Opportunities and ChallengesELECTROANALYSIS, Issue 12 2007Jonathan Abstract Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization, and label-free operation. Unlabeled DNA and protein targets can be detected by monitoring changes in surface impedance when a target molecule binds to an immobilized probe. The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout. Various possible mechanisms for impedance change upon target binding are discussed. We critically summarize accomplishments of past label-free impedance biosensors and identify areas for future research. [source] Electrochemical Biosensors for Detection of Biological Warfare AgentsELECTROANALYSIS, Issue 3 2003Jasmin Shah Abstract This review discusses current development in electrochemical biosensors for detection of biological warfare agents. This could include bacteria, viruses and toxins that are aerosoled deliberately in air, food or water to spread terrorism and cause disease or death to humans, animals or plants. The rapid and unequivocal detection and identification of biological warfare agents is a major challenge for any government including military, health and other government agents. Reliable, specific characterization and identification of the microorganism from sampling location, either air, water, soil or others is required. This review will survey different types of electrochemical biosensors has been developed based on the following: i),Immunosensors ii),PCR (DNA base Sensor) iii),Bacteria or whole cell sensor and iv),Enzyme sensor. This article gives an overview of electrochemical biosensor for detection of biological warfare agents. Electrochemical biosensors have the advantages of sensitivity, selectivity, to operate in turbid media, and amenable to miniaturization. Recent developments in immunofiltration, flow injection, and flow-through electrochemical biosensors for bacteria, viruses, and toxin detection are reviewed. The current research and development in biosensors for biological warfare agents detection is of interest to the public as well as to the defense is also discussed. [source] Cover Picture: Electrophoresis 14'2010ELECTROPHORESIS, Issue 14 2010Article first published online: 21 JUL 2010 Issue no. 14 is a "mini special issue" on "Microscale Separation Methods for Metabolomics" comprising 9 manuscripts on metabolomics and 12 manuscripts on various topics in nucleic acids, biomarkers, proteomics, miniaturization, etc. Part I has 9 manuscripts on metabolomics featuring new technological developments and the potential of CE-MS, targeted analysis of one class of metabolites and non-targeted analysis, and data interpretation that is essential to acquire useful biological information. In short, the importance of CE and, generally, of microscale separation methods for metabolomics is rapidly increasing and the papers published in this issue give an overview of this field. Part II has 2 research papers on biomarkers while Part III is on various aspects of nucleic acids including but not limited to genotyping, PCR, SSCP, PCR and detection of DNA. Part IV describes various aspects of fundamentals and methodology in microfluidics, cell lysates by 2-DE, CE-LIF of plasmid DNA, whole blood assay of trypsin activity, etc. [source] IEF in microfluidic devicesELECTROPHORESIS, Issue 5 2009Greg J. Sommer Abstract IEF is one of the most powerful and prevalent techniques used in separation sciences. The power of IEF comes from the fact that it not only separates analytes based on their pI but also focuses them into highly resolved bands. In line with the miniaturization trend spurring the analytical community, the past decade has yielded a wealth of research focused on implementing IEF in microfluidic chip-based formats (,IEF). Scaling down the separation technique provides several advantages such as reduced sample sizes, assay automation, and significant improvements in assay speed without sacrificing separation performance. Besides presenting microscale adaptations of standard schemes, researchers have also developed improved detection techniques, demonstrated novel ,IEF assays, and incorporated ,IEF with other analytical methods for achieving on-chip multidimensional separations. This review provides a brief historical outline of IEF's beginnings, theoretical incentives driving miniaturization of the methodology, a thorough synopsis of ,IEF publications to date, and an outlook to the future. [source] Cover Picture: Electrophoresis 2'09ELECTROPHORESIS, Issue 2 2009Article first published online: 9 FEB 200 Regular issues provide a wide range of research and review articles covering all aspects of electrophoresis. Here you will find cutting-edge articles on methods and theory, instrumentation, nucleic acids, CE and CEC, miniaturization and microfluidics, proteomics and two-dimensional electrophoresis. Issue no. 2 has a "Fast Track" paper on the attomole protein analysis by capillary isoelectric focusing (CIEF) with LIF detection based on a post-column sheath flow cuvette employing Chromeo P503 as a fluorogenic reagent for protein labeling before CIEF analysis. Further selected topics of issue 2 are: Influence of image-analysis software on quantitation of two-dimensional gel electrophoresis data A PDMS sheath flow cuvette for high-sensitivity LIF measurements in CE [source] Cover Picture: Electrophoresis 24'2008ELECTROPHORESIS, Issue 24 2008Article first published online: 7 JAN 200 This issue is a paper symposium on "Miniaturization and Microfluidics" with "30 exciting contributions, spanning reviews, original research papers and short communications. The articles are arranged in themed groups reflecting the variety of topics that characterize the fields of miniaturization, micro-total analysis systems and lab-on-a-chip devices. Fundamentals and theoretical investigations go hand in hand with new instrumentation and new technological developments, often finding their application within the areas of genomics, proteomics, metabolomics, cellomics and so forth. The life sciences continue to be the dominating "playground" for miniaturized systems. In addition, issue 24 has a "Fast Track" article on dielectrophoretic detection and quantification of hybridized DNA molecules on nano-genetic particles. [source] Cover Picture: Electrophoresis 22'2008ELECTROPHORESIS, Issue 22 2008Article first published online: 26 NOV 200 Regular issues provide a wide range of research and review articles covering all aspects of electrophoresis. Here you will find cutting-edge articles on methods and theory, instrumentation, nucleic acids, CE and CEC, miniaturization and microfluidics, proteomics and two-dimensional electrophoresis. Selected topics of issue 22 are: Microfluidics: Applications for analytical purposes in chemistry and biochemistry ((http://doi.wiley.com/10.1002/elps.200800121)) Simultaneous laser-induced fluorescence and retro-reflected beam interference detection for CE ((http://doi.wiley.com/10.1002/elps.200800292)) Quantitative Proteomics by Fluorescent Labeling of Cysteine Residues using a Set of Two Cyanine-based or Three Rhodamine-based Dyes ((http://doi.wiley.com/10.1002/elps.200800092)) Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences ((http://doi.wiley.com/10.1002/elps.200800400)) Identification of inorganic ions in post-blast explosive residues using portable capillary electrophoresis instrumentation and capacitively-coupled contactless conductivity detection ((http://doi.wiley.com/10.1002/elps.200800226)) [source] Cover Picture: Electrophoresis 18'2008ELECTROPHORESIS, Issue 18 2008Article first published online: 10 OCT 200 Issue number 18 is a Special Issue on "CE and CEC Innovations" consisting of 26 papers arranged into six sections. This Issue has one review article on the application of modern separation techniques in metabonomics, while the remaining articles pertain to microfluidics and miniaturization, novel trends in fundamentals and methodologies, various aspects of capillary coatings, open tubular CEC, MIP-CE, polar and nonpolar monoliths, MEEKC, MEKC and enantioseparations by CE and CEC. In addition Issue 18 has a "Fast Track" article on the CE-based assay of sirtuin enzymes. [source] Cover Picture: Electrophoresis 15/2008ELECTROPHORESIS, Issue 15 2008Article first published online: 24 JUL 200 Regular issues provide a wide range of research and review articles covering all aspects of electrophoresis. Here you will find cutting-edge articles on methods and theory, instrumentation, nucleic acids, CE and CEC, miniaturization and microfluidics, proteomics and two-dimensional electrophoresis. Selected topics of issue 15 are: The application of perfluorooctanoate to investigate trimerization of the human immunodeficiency virus-1 gp41 ectodomain by electrophoresis Metabolic fingerprinting of schistosoma mansoni infection in mice urine with capillary electrophoresis Supercritical fluid extraction as an on-line clean-up technique for determination of riboflavin vitamins in food samples by capillary electrophoresis with fluorimetric detection A two-step electro-dialysis method for DNA purification from polluted metallic environmental samples. [source] | ||||||||||||||||||||||