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Microscopic Slides (microscopic + slide)

Distribution by Scientific Domains
Medical Sciences80%

Selected Abstracts

Intrapulmonary lymph nodes in South African miners,an autopsy survey,

AMERICAN JOURNAL OF INDUSTRIAL MEDICINE, Issue 4 2007
Koichi Honma MD
Abstract Background Knowledge on intrapulmonary lymph nodes (IPLNs) is still limited. Progress in imaging techniques has enabled easier, more frequent visualization of IPLNs so that a more comprehensive understanding of these nodes is necessary. Methods Microscopic slides of lung tissue from 2,337 dust-exposed South African miners autopsied in 1975 were reviewed to identify IPLNs. The prevalence of IPLNs was calculated and histopathological changes in IPLNs and the surrounding lung parenchyma were described. Pathological changes of IPLNs were correlated with those of the surrounding pulmonary parenchyma. Results IPLNs were found in 86 of the miners (3.7%). Silicotic nodules were seen in IPLNs in 32 of the 86 cases (37.2%), in the majority of which (21/32; 65.6%) the surrounding lung parenchyma was almost normal. Conclusion IPLNs are not uncommon among dust-exposed individuals. Silicotic fibrosis of IPLNs appears to precede pulmonary parenchymal disease. Am. J. Ind. Med. 2007. © 2007 Wiley-Liss, Inc. [source]

Spatial refractive index measurement of porcine artery using differential phase optical coherence microscopy

LASERS IN SURGERY AND MEDICINE, Issue 10 2006
Jeehyun Kim PhD
Abstract Background and Objectives We describe a methodology to record spatial variation of refractive index of porcine renal artery using differential phase optical coherence microscopy (DP-OCM). Study Design/Materials and Methods The DP-OCM provides quantitative measurement of thin specimen phase retardation and refractive index by measuring optical path-length changes on the order of a few nanometers and with a lateral resolution of 3 µm. The DP-OCM instrumentation is an all-fiber, dual-channel Michelson interferometer constructed using a polarization maintaining (PM) fiber. Results Two-dimensional en face dual-channel phase images are taken over a 150,×,200 µm region on a microscopic slide, and the images are reconstructed by plotting a two-dimensional refractive index map as the OCM beam is moved across the sample. Conclusions Because the DP-OCM can record transient changes in the optical path-length, the system may be used to record quantitative optical path-length alterations of tissue in response to various stimuli. A fiber-based DP-OCM may have the potential to substantially improve in vivo imaging of individual cells for a variety of clinical diagnostics, and monitoring applications. Lasers Surg. Med. © 2006 Wiley-Liss, Inc. [source]

Clinical applications of laser scanning cytometry

CYTOMETRY, Issue 3 2002
Attila Tárnok
Abstract This study reviews existing and potential clinical applications of laser scanning cytometry (LSC) and outlines possible future developments. LSC provides a technology for solid phase cytometry. Fluorochrome-labeled specimens are immobilized on microscopic slides that are placed on a conventional epifluorescence microscope and analyzed by one or two lasers. Data comparable to flow cytometry are generated. In addition, the position of each event is recorded, a feature that allows relocalization and visualization of each measured event. The major advantage of LSC compared with other cytometric methods is the combination of two features: (a) the minimal clinical sample volume needed and (b) the connection of fluorescence data and morphological information for the measured event. Since the introduction of LSC, numerous methods have been established for the analysis of cells, cellular compartments, and tissues. Although most cytometric methods use only two or three colors, the characterization of specimens with up to five fluorochromes is possible. Most clinical applications have been designed to determine ploidy and immunophenotype; other applications include analyses of tissue biopsies and sections, fluorescence in situ hybridization, and the combination of vital and nonvital information on a single-cell basis. With the currently available assays, LSC has proven its wide spectrum of clinical applicability in slide-based cytometry and can be introduced as a standard technology in multiple clinical settings. Cytometry (Clin. Cytometry) 50:133,143, 2002. © 2002 Wiley-Liss, Inc. [source]

Evaluation of coexistence of the Human Herpesvirus type 8 (HHV-8) infection and pemphigus

INTERNATIONAL JOURNAL OF DERMATOLOGY, Issue 7 2010
Naser Tayyebi Meibodi Associate Professor
Background, Human Herpesvirus type 8 (HHV-8) is a new member of the herpes virus family, first found in the tissue of acquired immune deficiency syndrome (AIDS)-related Kaposi's sarcoma. Environmental factors including viral infection may play a role in the onset and/or development of pemphigus. Some studies based on polymerase chain reaction findings suggest an association between HHV-8 and pemphigus. The aim of this study is investigation of the association of pemphigus with HHV-8 and the relationship between inflammatory and acantholytic cells with HHV-8 infection. Methods, Tissue-extracted DNA from 41 paraffin-embedded skin tissues from patients first presenting with pemphigus was tested using nested PCR for the presence of HHV-8. A total of 37 cases had pemphigus vulgaris (PV) and 4 patients had pemphigus foliaceus (PF). For our control group, normal skin of 21 cosmetic surgery patients were included. Furthermore, microscopic slides with H&E staining were evaluated for the number of inflammatory and acantholytic cells per microscopic field. Results, Skin lesions from 13 of 37 patients (35.1%) with PV and 2 of 4 cases (50%) with PF were positive for HHV-8 DNA. All specimens in our control group were negative for this virus. Conclusion, HHV-8 infection might be a contributing factor in the initiation or development of pemphigus. [source]

Scleroderma ,en coup de sabre' and progressive facial hemiatrophy.

JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY & VENEREOLOGY, Issue 4 2002
Is it possible to differentiate them?
Abstract The aim was to be able to evaluate the diagnosis of two diseases by a consensus of clinical opinion used in the Department of Dermatology of the National Institute of Paediatrics in Mexico City. To differentiate between scleroderma ,en coup de sabre' (SCS) and progressive facial hemiatrophy (PFH), colour slides of 13 patients diagnosed as SCS and nine as PFH were examined by two dermatologists and microscopic slides by two pathologists. In both cases, the slides were randomly presented and no clinical information was given. The clinical and histopathological findings were statistically compared with two-tailed tests and , = 0.05. , coefficients were obtained to evaluate the concordance between dermatologists, pathologists, and in terms of the consensus diagnosis. The usefulness of photographic assessment is limited by the inability to palpate the consistency of lesions. The most important clinical feature that differentiated both conditions was cutaneous sclerosis present in eight of 13 patients with SCS and in none of the PFH patients (P < 0.005). Other clinical features more frequently found in SCS were cutaneous hyperpigmentation and alopecia. The more frequent clinical features in PFH were total hemifacial involvement and ocular changes. Statistically significant histopathological features were: connective tissue fibrosis present in all cases with SCS and two of nine patients with PFH (P < 0.0002); adnexal atrophy present in 11 of 13 patients with SCS, and in three of nine with PFH (P < 0.02), and mononuclear cell infiltrates in all patients with SCS cf. six with PFH (P < 0.05). Our results suggest that in most cases it is possible to differentiate SCS from PFH based on clinicopathological findings. [source]