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Microsatellite Primers (microsatellite + primer)
Terms modified by Microsatellite Primers Selected AbstractsMicrosatellite primers for relatedness and population structure in great frigatebirds (Pelecaniformes: Fregatidae)MOLECULAR ECOLOGY RESOURCES, Issue 6 2008DONALD C. DEARBORN Abstract Eighteen moderately polymorphic microsatellite loci were isolated and characterized for great frigatebirds Fregata minor. Polymorphism for the 12 dinucleotide and six tetranucleotide markers was assessed in 23 birds from a Hawaii population. Allelic diversity ranged from two to 12 (mean 5.9), with observed heterozygosity from 0.304 to 0.956 (mean 0.637). Three loci showed homozygote excess, possibly due to null alleles. One additional pair of loci exhibited strong gametic disequilibrium. Thus, at least 14 loci will be useful for studies of relatedness and population structure. [source] Isolation and characterization of microsatellite loci in Coccoloba cereifera (Polygonaceae), an endangered species endemic to the Serra do Cipó, BrazilMOLECULAR ECOLOGY RESOURCES, Issue 4 2008RENNAN G. MOREIRA Abstract Microsatellite primers were isolated from the microendemic and threatened species Coccoloba cereifera, a shrub known only from a small region in the Serra do Cipó, Brazil. Thirteen primer pairs amplifying perfect and imperfect microsatellite regions were tested in 40 individuals from the one known occurrence of the species. Number of alleles ranged from two to six and levels of observed heterozygosities ranged from 0.21 to 0.95. These markers will be useful for the analysis of questions concerning population genetic structure and will assist in providing information for future conservation management programmes. [source] Microsatellite primers for the Atlantic coastal killifish, Fundulus heteroclitus, with applicability to related Fundulus speciesMOLECULAR ECOLOGY RESOURCES, Issue 2 2005STEPHANIE M. ADAMS Abstract The mummichog (Fundulus heteroclitus), a common Atlantic coastal killifish, is a model vertebrate species for the study of molecular genetic variation in natural populations and of environmental toxicology. We report the development of a set of 20 microsatellite loci in this species. Average expected heterozygosity across all loci was 0.84 (range: 0.60,0.97), revealing a high level of variability at most loci. A survey of seven additional Fundulus species yielded one or two robust amplification products in over half (63%) of the species,primer combinations tested. Therefore, many of these loci will also prove useful in studies of other members of the genus Fundulus. [source] False sex-linked microsatellite primer for Phoca vitulinaMARINE MAMMAL SCIENCE, Issue 2 2008Jason K. Herreman [source] Social Dominance among Male Meadow Voles is Inversely Related to Reproductive SuccessETHOLOGY, Issue 10 2006Mark D. Spritzer Intrasexual selection can occur through direct aggressive interactions between males for access to females. We tested the relationship between social dominance and male reproductive success among meadow voles, Microtus pennsylvanicus. Dominance ranks of wild-caught males were determined using neutral arena trials, with the winner of two of three trials considered dominant. These males were then released into field enclosures and allowed to visit females housed in nestboxes for 8 wk, and males' home range sizes were determined using weekly grid trapping. Male reproductive success was determined using molecular paternity analysis (six microsatellite primers) for all pups born during the field experiment. Males with higher dominance ranks had larger home ranges. However, male dominance rank was not predictive of the number of total visits to females' nestboxes or the number of visits to each male's most frequently visited nestbox. Males that made more visits to nestboxes sired more litters. Males that had higher dominance ranks sired fewer litters. These results suggest that there is a reproductive disadvantage to having higher dominance rank among male meadow voles. [source] Cross-species amplification of Lolium microsatellites in Poa sspGRASSLAND SCIENCE, Issue 3 2006Bryan Kindiger Abstract Cross-species amplification of 47 Lolium ssp. microsatellite primers were evaluated across eight Poa species or subspecies. Of the 47 evaluated Lolium simple sequence repeat (SSR) primer pairs examined, 18 generated one or more amplification products. Of these, only two resulted in the identification of Poa ssp. microsatellite motifs, of which only one was complementary to the microsatellite motif identified in Lolium. Though few Poa ssp. microsatellite regions were identified, several of the amplification products were polymorphic within and across the Poa ssp. and could be utilized as markers in Poa ssp. intergeneric hybrid studies. Results of the research suggest the use of Lolium microsatellite derived primers to identify complementary SSR regions in Poa is not an effective approach for the development of microsatellite markers in Poa. [source] Analysis of two Chinese yak (Bos grunniens) populations using bovine microsatellite primersJOURNAL OF ANIMAL BREEDING AND GENETICS, Issue 4 2003Wang Minqiang Summary Two Chinese domestic yak populations representing the Plateau type and the Huanhu Alpine type were analysed with 12 bovine microsatellite primers. All primer pairs functioned in the yak genome and polymorphism was found at all loci. The allele size ranges and frequencies of the two yak populations were similar and there was considerable overlap with the allele size ranges observed in cattle. Data for European cattle breeds was obtained from the Cattle Diversity Database (CaDBase) to interpret the heterozygosity and genetic distance estimates in yak populations. Heterozygosity estimated for the two yak populations was comparable with that of European cattle while Nei's Genetic Distance DA between the two yak populations was less than distances between the most closely related German cattle breeds. Bovine microsatellite primers proved to be a valuable tool for characterization of yak populations. Zusammenfassung Untersuchungen von zwei chinesischen Yak (Bos grunniens) Herden mittels Primersequenzen von Rindermikrosatelliten Primersequenzen von Rindermikrosatelliten wurden verwendet, um zwei chinesische Yak-Herden zu analysieren, eine vom Plateau-Typ und eine andere vom Huanhu-Bergtyp. Alle 12 Primerpaare führten zu polymorphen Amplifikationsprodukten mit den DNA-Proben der Yaks. Der Allelgrößenbereich und die Allelfrequenzen aller 12 Marker waren in den beiden Yak-Herden sehr ähnlich. Zum Vergleich wurden Typisierungsergebnisse der 12 Mikrosatellitengenorte von 15 europäischen Rinderassen aus der Rinderdiversitätsdatenbank CaDBase entnommen. Ein großer Teil der Yak-Allele hatte die gleiche Größe wie beim Rind und auch der geschätzte Heterozygotiegrad der beiden Yak-Populationen war vergleichbar mit dem durchschnittlichen Heterozygotiegrad der 15 Rinderrassen. Die genetische Distanz (Nei's DA) zwischen beiden Yak-Herden wurde jedoch geringer geschätzt als zwischen zwei Paaren eng verwandter Rinderrassen. Die Ergebnisse zeigen, dass Sequenzinformationen von Rindermikrosatellitenprimern für die Charakterisierung von Yak-Populationen auf der molekularen Ebene nützlich sein können. [source] Habitat differentiation vs. isolation-by-distance: the genetic population structure of Elymus athericus in European salt marshesMOLECULAR ECOLOGY, Issue 2 2003A.-C. Bockelmann Abstract We investigated genetic differentiation among populations of the clonal grass Elymus athericus, a common salt-marsh species occurring along the Wadden Sea coast of Europe. While E. athericus traditionally occurs in the high salt marsh, it recently also invaded lower parts of the marsh. In one of the first analyses of the genetic population structure in salt-marsh species, we were interested in population differentiation through isolation-by-distance, and among strongly divergent habitats (low and high marsh) in this wind- and water-dispersed species. High and low marsh habitats were sampled at six sites throughout the Wadden Sea. Based on reciprocal transplantation experiments conducted earlier revealing lower survival of foreign genotypes we predicted reduced gene flow among habitats. Accordingly, an analysis with polymorphic cross-species microsatellite primers revealed significant genetic differentiation between high and low marsh habitats already on a very small scale (< 100 m), while isolation-by-distance was present only on larger scales (60,443 km). In an analysis of molecular variance we found that 14% of the genetic variance could be explained by the differentiation between habitats, as compared to only 8.9% to geographical (isolation-by-distance) effects among six sites 2.5,443 km distant from each other. This suggests that markedly different selection regimes between these habitats, in particular intraspecific competition and herbivory, result in habitat adaptation and restricted gene flow over distances as small as 80 m. Hence, the genetic population structure of plant species can only be understood when considering geographical and selection-mediated restrictions to gene flow simultaneously. [source] Characterization and isolation of DNA microsatellite primers in hyrax species (Procavia johnstoni and Heterohyrax brucei, hyracoidea)MOLECULAR ECOLOGY, Issue 10 2000Gabriele Gerlach [source] Isolation and characterization of microsatellite primers for Ceratosolen fusciceps, the fig-pollinating wasp of Ficus racemosa, and amplification in two populationsMOLECULAR ECOLOGY RESOURCES, Issue 4 2009N. KOBMOO Abstract Ten microsatellite loci were isolated and characterized for Ceratosolen fusciceps, the pollinating wasp of Ficus racemosa, based on individuals from Xishuangbanna (China) and Chiangmai (Thailand). High levels of variation were found among loci for both populations; allelic number varied from two to 18 (respectively from two to 22), expected heterozygosities ranged from 0.362 to 0.890 (respectively from 0.430 to 0.930). [source] Characterization of novel microsatellites from Drosophila transversaMOLECULAR ECOLOGY RESOURCES, Issue 2 2009L. RÄISÄNEN Abstract We investigated a partial genomic library of Drosophila transversa for microsatellites and developed 12 markers for genetic analyses. This is the first time that microsatellite primers from the quinaria species group have been described. Four loci were cross-amplified in D. phalerata. Nine out of the 12 microsatellite markers developed are likely to be on the X chromosome. [source] Twelve microsatellite loci for marine and riverine tucuxi dolphins (Sotalia guianensis and Sotalia fluviatilis)MOLECULAR ECOLOGY RESOURCES, Issue 6 2007HAYDÉE A. CUNHA Abstract Twelve dinucleotide polymorphic microsatellite loci were isolated from the marine tucuxi dolphin (Sotalia guianensis). Levels of genetic diversity were assessed using 34 individuals from the coasts of Rio de Janeiro and Pará, Brazil. Numbers of alleles varied between two and 14, and observed and expected heterozygosities ranged from 0.040 to 0.704, and from 0.093 to 0.818, respectively. Moreover, eight of these loci were variable in the riverine tucuxi Sotalia fluviatilis. This is the first description of microsatellite primers from a dolphin that does not belong to the Delphininae. These loci are currently being used in the analysis of population structure of both tucuxi species. [source] Isolation and characterization of eight microsatellite loci from Picnic seabream (Acanthopagrus berda)MOLECULAR ECOLOGY RESOURCES, Issue 4 2006C. T. JEAN Abstract Acanthopagrus berda (Sparidae) is abundant in estuaries and inshore waters of the tropical Indo-Pacific oceans. Because of the drastic decrease in the species' wild populations, there is an urgent need to understand its population structure. Eight microsatellite loci were isolated from A. berda. An analysis of 50 individuals from Tapeng Lagoon, southwestern Taiwan demonstrated a low degree of diversity. The allelic numbers ranged from nine to 20. The observed heterozygosity (HO) ranged from 0.28 to 0.64 (mean, 0.45). We also investigated the prospective value of the microsatellite primers to examine whether they were applicable to related taxa. [source] Isolation and characterization of DNA microsatellite primers for Cynoscion acoupa, the most exploited sciaenid fish along the coast of BrazilMOLECULAR ECOLOGY RESOURCES, Issue 3 2006IZENI P. FARIAS Abstract The northern mangrove coast of Brazil comprises the Brazilian states of Amapá, Pará and Maranhão. Fishes from the coastal mangrove region form a principal component of the local fishery, and account for 58% of all fish landed. The most commonly landed fishes are those of the family Sciaenidae. Cynoscion acoupa is one of the most exploited sciaenid species, with over 600 tons landed each year. Therefore, we developed 17 microsatellite markers to investigate genetic variability and population structuring of this species along the coast of Brazil. [source] Isolation of microsatellite loci and cross-species amplifications in three gobiid fish of the genus PomatoschistusMOLECULAR ECOLOGY RESOURCES, Issue 3 2006PATRICK BERREBI Abstract Gobiids of the genus Pomatoschistus are increasingly being investigated as models for adaptation to coastal environments and for mating system studies. Among the dozen currently analysed species, microsatellite primers have been characterized only for Pomatoschistus minutus. This paper describes seven new polymorphic loci isolated from Pomatoschistus marmoratus and Pomatoschistus microps, two species that hybridize. Cross-species amplification was tested for these new loci, together with seven already published P. minutus loci. Systematic amplification of samples of each of the three species provided a first indication of their polymorphism. [source] Nine new polymorphic microsatellite loci for the amplification of archived otolith DNA of Atlantic cod, Gadus morhua L.MOLECULAR ECOLOGY RESOURCES, Issue 2 2006KLARA BJÖRG JAKOBSDÓTTIR Abstract Nine out of 22 microsatellite primers tested were successfully amplified on three samples of cod Gadus morhua L. (two contemporary and one archived otolith samples). All loci were polymorphic (5,23 alleles/locus). The average observed heterozygosity across loci and samples was 0.625, ranging from 0.294 to 0.895 at each locus. All loci were under Hardy,Weinberg equilibrium, except PGmo56 that showed significant excess of heterozygotes in all studied samples. The isolated loci were suitable for degraded DNA and therefore useful for conducting a long-term temporal study with DNA obtained from archived otoliths of cod. [source] Dinucleotide microsatellite primers designed for a critically endangered primate, the black lion tamarin (Leontopithecus chrysopygus)MOLECULAR ECOLOGY RESOURCES, Issue 2 2005BEATRIZ M. PEREZ-SWEENEY Abstract Black lion tamarin (BLT) monkeys (Leontopithecus chrysopygus) have suffered a severe reduction in their natural range and are consequently critically endangered. Because allozyme data showed very low levels of variation, it was not clear if these monkeys had much genetic diversity. We designed microsatellite primers for BLTs, and from them we identified nine polymorphic loci, seven of which were tested on golden lion tamarins (GLTs) (Leontopithecus rosalia). All of the seven polymorphic loci and two other monomorphic BLT loci were polymorphic in GLTs. The microsatellite markers identified here are directly applicable to ongoing lion tamarin population and conservation genetics studies. [source] New microsatellite primers for Daphnia galeata mendotaeMOLECULAR ECOLOGY RESOURCES, Issue 4 2004JENNIFER A. FOX Abstract In order to study genetic changes in populations of Daphnia galeata mendotae, I characterized seven polymorphic microsatellite loci. Primers to amplify these loci were tested on individual eggs from the resting egg bank of Onondaga Lake, NY. Levels of polymorphism and cross-amplification in D. g. galeata indicate that they will be useful markers for ecological genetic studies on both adults and diapausing eggs of these species. [source] Identification and characterization of 10 microsatellite primers for the calanoid freshwater copepods Eudiaptomus gracilis and E. graciloides using enriched genomic librariesMOLECULAR ECOLOGY RESOURCES, Issue 3 2004MARTINA ZELLER Abstract The calanoid copepods Eudiaptomus gracilis and Eudiaptomus graciloides are widespread among European lakes. We constructed enriched genomic libraries for both species in order to isolate and characterise microsatellite markers. The obtained 7 polymorphic microsatellite-loci for E. gracilis and 3 for E. graciloides are the first for any freshwater copepod. They display an expected heterozygosity ranging from 0.60 to 0.96 and 0.63 to 0.94 and observed heterozygosity ranging from 0.53 to 0.87 and 0.57 to 0.87, respectively. We were not able to cross-amplify the isolated loci across species, indicating long divergence among both congeneric species despite morphological similarity. [source] Characterization and isolation of DNA microsatellite primers in the spiny dogfish (Squalus acanthias)MOLECULAR ECOLOGY RESOURCES, Issue 3 2004LINDA McCAULEY Abstract The North Atlantic spiny dogfish (Squalus acanthias) population has been declining since the 1980s. Proper management of this population is essential as dogfish are prone to rapid collapse based on the unique biology of this species. We characterized eight microsatellite loci for spiny dogfish to use in determining the genetic structure of this species along the Atlantic coast of the USA. [source] Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangleMOLECULAR ECOLOGY RESOURCES, Issue 1 2002Andrew J. Lowe Abstract We present a new set of 12 highly polymorphic simple sequence repeat primer sequences for use with Brassica species. These new primers, and four from A.K.S. SzewcMcFadden and colleagues, were tested in four Brassica species (B. rapa, B. napus, B. oleracea and B. nigra). Most primers successfully amplified products within all species and were polymorphic. Due to the risk of gene flow from GM oilseed rape to its wild relatives, hybrid formation in the Brassicaceae is of great interest. We identify six primer pairs as specific to the A, B or C genomes that could be used to identify such hybrids. [source] Recurrent selection of cocoa populations in Côte d'Ivoire: comparative genetic diversity between the first and second cyclesPLANT BREEDING, Issue 5 2009N. D. Pokou Abstract In Côte d'Ivoire, the cocoa breeding programme has been based on the creation of hybrids between different genetic groups. From 1990 onward, a reciprocal recurrent selection programme has been set up with the purpose of improving simultaneously the characteristics of the two main genetic groups: Upper Amazon Forastero (UA) and a mixture of Lower Amazon Forastero (LA) and Trinitario (T). Based on data obtained from 12 microsatellite primers, the genetic diversity and genetic distances of the parental populations used in the first and second selection cycles are presented. The results revealed that the diversity of populations UA0 and UA1 on the one hand and (LA+T)0 and (LA+T)1 on the other is similar. The genetic distances were small between the parental populations used for the first and second cycles. Genetic diversity was greater in the UA group than in the LA+T group. The number of rare and of private alleles was reduced for both genetic groups, as well as the number of the frequent alleles in the LA+T group. [source] Microsatellite marker for yellow rust resistance gene Yr5 in wheat introgressed from spelt wheatPLANT BREEDING, Issue 6 2002Q. Sun Abstract Yellow rust of wheat caused by Puccinia striiformis f sp. tritici has been periodically epidemic and severely damaged wheat production in China and throughout the world. Breeding for resistant cultivars has been proved to be an effective way to resolve the problem. A yellow rust resistance gene, Yr5, derived from Triticum spelta shows immunity or high resistance to the most popular isolates Tiaozhong 30 and 31 in China. Establishment of DNA markers for the Yr5 gene will facilitate marker-assisted selection and gene pyramiding in the breeding programme. Since the Yr5 gene was cytologically located on the long arm of chromosome 2B, By33, the donor of Yr5, was crossed and backcrossed with the susceptible line 441, and BC3F2 and BC3F3 segregating populations were screened for polymorphism by using 11 microsatellite primers mapped on chromosome 2B. A marker, Xgwm501-195 bp/160 bp, was found to be linked to Yr5, with a genetic distance of 10.5-13.3 cM. [source] Chicken microsatellite primers are not efficient markers for Japanese quailANIMAL GENETICS, Issue 1 2001M. Inoue-Murayama Domestic fowl or chicken (Gallus gallus) and Japanese quail (Coturnix japonica) belong to the family Phasianidae. The exchange of marker information between chicken and quail is an important step towards the construction of a high-resolution comparative genetic map in Phasianidae, which includes several poultry species of agricultural importance. We tested chicken microsatellite markers to see if they would be suitable as genetic linkage markers in Japanese quail. Twenty-six per cent (31/120) of chicken primers amplified individual loci in Japanese quail and 65% (20/31) of the amplified loci were found to be polymorphic. Eleven of the polymorphic loci were excluded as uninformative because of the lack of amplification in some individuals or high frequency of nonspecific amplification. The sequence information of the remaining nine loci revealed six of them to contain microsatellites that were nearly identical with those of the orthologous regions in chicken. For these six loci, allele frequencies were estimated in 50 unrelated quails. Although the very few chicken markers that do work well in quail could be used as anchor points for a comparative mapping, most chicken markers are not useful for studies in quail. Therefore, more effort should be committed to developing quail-specific markers rather than attempting to adapt chicken markers for work in quail. [source] | ||||||||||