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Microsatellite DNA Markers (microsatellite + dna_marker)
Kinds of Microsatellite DNA Markers Selected AbstractsMicrosatellite DNA markers reveal genetic differentiation among populations of Glossina palpalis gambiensis collected in the agro-pastoral zone of Sideradougou, Burkina FasoINSECT MOLECULAR BIOLOGY, Issue 4 2000P. Solano Abstract Intraspecific genetic variability of Glossina palpalis gambiensis in the area of Sideradougou, Burkina Faso, was studied using polymorphic microsatellite DNA markers. This genetic study was combined with other epidemiological information on the same tsetse: bloodmeal identification, dissection of tsetse and molecular characterization of the trypanosomes detected. There was significant genetic differentiation among flies caught only a few kilometers apart, within the same riverine habitat. These distinct subpopulations were also differentially infected by trypanosomes. In part of the study area, a Factorial Correspondence Analysis undertaken on the genotypes allowed us to detect a Wahlund effect, suggesting the presence of tsetse originating from different source populations coming from two distinct drainage systems. The apparent structuring of populations of G. palpalis gambiensis is discussed relative to appropriate strategies to control African Trypanosomosis. [source] Microsatellite DNA markers for the study of Allegheny woodrat (Neotoma magister) populations and cross-species amplification in the genus NeotomaMOLECULAR ECOLOGY, Issue 6 2000S. B. Castleberry [source] Microsatellite DNA markers for population-genetic studies of blue mackerel (Scomber australasicus) and cross-specific amplification in S. japonicusMOLECULAR ECOLOGY RESOURCES, Issue 3 2009C. Y. TANG Abstract Blue mackerel (Scomber australasicus) is targeted by large-scale purse-seiners in the western North Pacific, and its stock structure is still contentious. Herein, we described 10 polymorphic microsatellite loci for blue mackerel. The number of alleles among 32 individuals surveyed ranged from five to 27 (average of 16.2 alleles per locus). Departures from Hardy,Weinberg expectation were observed at two loci. Cross-specific amplification in the congener, S. japonicus, was successful, except for one locus, revealed to be diagnostic for these congeners. These microsatellite loci will be useful tools to address queries in population genetic structure, fishery management unit and taxonomic species status in the genus Scomber. [source] Microsatellite DNA markers for three toad-headed lizard species (Phrynocephalus vlangalii, P. przewalskii and P. guttatus)MOLECULAR ECOLOGY RESOURCES, Issue 2 2009AIBIN ZHAN Abstract To assess the impact of natural landscapes on the population structure of lizards, 10 polymorphic microsatellite DNA markers were developed for the Qinghai toad-headed lizard, Phrynocephalus vlangalii. The number of alleles at these informative loci ranged from four to 28. The novel markers and those previously developed for Phrynocephalus przewalskii were cross-tested among three toad-headed lizard species P. vlangalii, P. przewalskii and P. guttatus. A high cross-utility rate of more than 58% was observed among these three species. These markers are expected to be useful tools for taxonomic considerations as well as population genetic analysis and future conservation management. [source] Polymorphic microsatellite loci in the widespread amphidromous goby Sicyopterus lagocephalus and cross-genus amplification among SicydiinaeMOLECULAR ECOLOGY RESOURCES, Issue 2 2009T. B. HOAREAU Abstract Microsatellite DNA markers were isolated in an amphidromous goby (Sicyopterus lagocephalus) from a partial genomic library enriched for AC repeats. Eight microsatellites were highly polymorphic with six to 33 alleles per locus and expected heterozygosities ranging from 0.53 to 0.97. Cross-species amplifications were performed within the sub-family Sicydiinae by genotyping individuals from two species of the genus Cotylopus. Some of these loci were successfully amplified and showed polymorphism in the second genus. [source] Microsatellite DNA markers for the Chinese wood frog (Rana chensinensis) and tests for their cross-utility in 15 ranid frog speciesMOLECULAR ECOLOGY RESOURCES, Issue 5 2008AIBIN ZHAN Abstract We developed 22 microsatellite markers for the Chinese wood frog (Rana chensinensis) to study the impact of landscape features on its population structure. Thirty-four individuals from one breeding site were examined and 14 loci were polymorphic. The number of alleles, expected heterozygosity and observed heterozygosity varied from two to 14, from 0.0833 to 0.9118, and from 0.1376 to 0.8667, respectively. Cross-species amplification was tested for 15 ranid frog species. The Plateau brown frog, Rana kukunoris (n = 23), was successfully amplified at 18 loci, and 15 were polymorphic with number of alleles varying from two to 18. Ten other species were also amplified at a limited number of loci. [source] Microsatellite DNA markers for the snow vole (Chionomys nivalis)MOLECULAR ECOLOGY RESOURCES, Issue 3 2008P. WANDELER Abstract A total of 14 dinucleotide microsatellite loci were characterized in the snow vole (Chionomys nivalis). Allelic polymorphism across all loci and 28 individuals representing a single population in the Swiss Alps was high (mean = 10.1 alleles). No significant linkage disequilibrium between pairs of loci and no departure from Hardy,Weinberg equilibrium were found. These loci will be useful for describing mating systems and population structure and to investigate the genetic consequences of a species living in a highly fragmented habitat. [source] Microsatellite DNA markers for the aphid parasitoid Lysiphlebus fabarum and their applicability to related speciesMOLECULAR ECOLOGY RESOURCES, Issue 6 2007CHRISTOPH SANDROCK Abstract The aphid parasitoid Lysiphlebus fabarum is suspected to form distinct, host-associated lineages and exhibits poorly understood variation in reproductive mode including thelytokous and arrhenotokous populations. As a tool to study these issues, we developed 11 polymorphic microsatellite markers. The observed number of alleles ranged from two to 35, and the observed heterozygosity ranged from 0.01 to 0.64. Cross-species amplification tests demonstrated their utility for several congeners, but revealed very limited applicability to more distantly related species. [source] Microsatellite DNA markers for the study of Atlantic salmon (Salmo salar) kinship, population structure, and mixed-fishery analysesMOLECULAR ECOLOGY RESOURCES, Issue 1 2005TIM L. KING Abstract Eleven microsatellite DNA loci were identified and characterized for Atlantic salmon (Salmo salar) collected from the Penobscot River, Maine, USA and the River Nith, Scotland, UK. The markers revealed high levels of genetic diversity (seven to 48 alleles per locus), heterozygosity (to 100%), and allelic heterogeneity (all comparisons). Considerable differentiation was observed as the genetic distance (chord) between the two collections was 0.680 and the pairwise FST, 0.12, was highly significant. These findings are consistent with patterns of continental-level differentiation observed previously using an alternate suite of microsatellite loci. Locus-by-locus analyses of molecular variance suggested that most markers were suitable for delineating kinships and population genetic structure. [source] Microsatellite DNA markers for the study of horseshoe crab (Limulus polyphemus) population structureMOLECULAR ECOLOGY RESOURCES, Issue 3 2004TIM L. KING Abstract Twenty-two microsatellite DNA loci were identified and characterized for horseshoe crabs (Limulus polyphemus) collected from two Atlantic coast and one Gulf of Mexico site. These markers revealed a high degree of genetic diversity (8,35 alleles per locus), heterozygosity (25.0% to 100.0%), and allelic heterogeneity (69.8% of comparisons). Considerable regional differentiation was observed as genetic distances (chord) ranged between 0.25 and 0.45, and all FST values (0.014,0.092) were significant. These preliminary findings are consistent with patterns of regional differentiation observed using allozyme variation and contradictory to findings of limited gene flow reported for sequence variation at the mitochondrial DNA COI region. [source] Microsatellite DNA markers for the ground beetle Pterostichus oblongopunctatus F.MOLECULAR ECOLOGY RESOURCES, Issue 1 2004M. Lagisz Abstract Six microsatellite DNA loci were isolated from the ground beetle Pterostichus oblongopunctatus F. (Coleoptera, Carabidae) to study population structure. Each locus was polymorphic, with the number of alleles ranging from three to six. Observed heterozygosity varied between 0.20 and 0.67. All six loci were tested for amplification in the closely related P. quadrifoveolatus and they were shown to be polymorphic. The primers developed were used in multiplex polymerase chain reactions. [source] Characterization of polymorphic microsatellite markers in the adder, Vipera berusMOLECULAR ECOLOGY RESOURCES, Issue 1 2003M. Carlsson Abstract Microsatellite DNA markers can yield sufficient resolution for individual identification as well as provide genetic information on a larger, interpopulational scale. Here we present details on six microsatellite primer pairs developed for the adder, Vipera berus. The number of alleles found varied between 2 and 38 per locus. The objectives behind developing these markers included assessment both of paternity and population histories from different parts of the species' range. Cross-species amplification indicated that these markers may also be useful for studies of other species within the Viperidae family. [source] Microsatellite DNA markers for two endemic ground beetles: Carabus punctatoauratus and C. solieriMOLECULAR ECOLOGY RESOURCES, Issue 4 2002S. Garnier Abstract We isolated and characterized nine and five polymorphic microsatellite loci in the respective ground beetles Carabus punctatoauratus and C. solieri. We tested cross-species amplification of all these loci plus six isolated in C. solieri and for which primers sequences were soon published. From these combined analyses, we obtained 14 and 17 polymorphic markers, respectively, for C. punctatoauratus and C. solieri. [source] Microsatellite DNA markers for a grasshopper: Prionotropis hystrix rhodanica (Orthoptera, Pamphagidae)MOLECULAR ECOLOGY RESOURCES, Issue 3 2002R. Streiff Abstract Microsatellite loci were isolated from Prionotropis hystrix rhodanica, an endangered grasshopper species, endemic to a steppic area southeast of France. Six polymorphic loci were obtained from an enriched partial genomic libray. A high genetic diversity was observed at all loci, with an observed number of alleles ranging from seven to 29, and an observed heterozygosity between 0.71 and 0.90. Test of cross-amplifications on two closely related taxa are presented. [source] Effects of environmental pollution on microsatellite DNA diversity in wood mouse (Apodemus sylvaticus) populationsENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 11 2005Veerle Berckmoes Abstract Ten microsatellite DNA loci were surveyed to investigate the effects of heavy metal pollution on the genetic diversity and population genetic structure of seven wood mouse (Apodemus sylvaticus) populations along a heavy metal pollution gradient away from a nonferrous smelter in the south of Antwerp (Flanders, Belgium). Analysis of soil heavy metal concentrations showed that soil Ag, As, Cd, Cu, and Pb decreased with increasing distance from the smelter. Genetic analyses revealed high levels of genetic variation in all populations, but populations from the most polluted sites in the gradient did not differ from those of less-polluted sites in terms of mean observed and expected heterozygosity level and mean allelic richness. No correlation was found between measures of genetic diversity and the degree of heavy metal pollution. However, an analysis of molecular variance and a neighbor-joining tree suggested a contamination-related pattern of genetic structuring between the most polluted and less polluted sites. Pairwise FST values indicated that populations were significantly genetically differentiated, and assignment tests and direct estimates of recent migration rates suggested restricted gene flow among populations. Additionally, genetic differentiation increased significantly with geographical distance, which is consistent with an isolation-by-distance model. We conclude that, at least for our microsatellite DNA markers, genetic diversity in the studied wood mouse populations is not affected greatly by the heavy metal pollution. [source] Microsatellite DNA markers reveal genetic differentiation among populations of Glossina palpalis gambiensis collected in the agro-pastoral zone of Sideradougou, Burkina FasoINSECT MOLECULAR BIOLOGY, Issue 4 2000P. Solano Abstract Intraspecific genetic variability of Glossina palpalis gambiensis in the area of Sideradougou, Burkina Faso, was studied using polymorphic microsatellite DNA markers. This genetic study was combined with other epidemiological information on the same tsetse: bloodmeal identification, dissection of tsetse and molecular characterization of the trypanosomes detected. There was significant genetic differentiation among flies caught only a few kilometers apart, within the same riverine habitat. These distinct subpopulations were also differentially infected by trypanosomes. In part of the study area, a Factorial Correspondence Analysis undertaken on the genotypes allowed us to detect a Wahlund effect, suggesting the presence of tsetse originating from different source populations coming from two distinct drainage systems. The apparent structuring of populations of G. palpalis gambiensis is discussed relative to appropriate strategies to control African Trypanosomosis. [source] Genetic restoration of a stocked brown trout Salmo trutta population using microsatellite DNA analysis of historical and contemporary samplesJOURNAL OF APPLIED ECOLOGY, Issue 4 2006MICHAEL M. HANSEN Summary 1Gene flow from domesticated to wild populations is a major threat to wild salmonid fish. However, few studies have addressed how populations could be restored after admixture has occurred. We analysed the prospects for restoring the previously intensively stocked brown trout population of the Skjern River, Denmark, by identifying remaining non-admixed individuals to be used for supportive breeding. 2We analysed microsatellite DNA markers in historical (1940,50s) and contemporary (1992,2004) samples from the Skjern River system, from the strain of domesticated trout previously used for stocking, and from the neighbouring Storå River. We analysed admixture proportions to estimate the genetic contribution by domesticated trout. We identified non-admixed trout using assignment tests, and further analysed the possible sources of indigenous trout by estimating contemporary migration among populations. 3Genetic differentiation between the historical Storå and Skjern river populations was low (,ST = 0·004), suggesting considerable gene flow in the past. The contemporary Skjern and Storå river populations and a supportive breeding brood stock were strongly admixed, but some non-admixed individuals nevertheless remained in the wild-caught samples. In addition, two resident populations in isolated tributaries were found to be indigenous. The indigenous anadromous individuals from the Skjern River were unlikely to have been recruited from either the isolated tributary populations or the neighbouring Storå River and were presumably derived from unidentified spawning sites in the river system. 4All but one non-admixed anadromous Skjern River trout were females, which we ascribed to sampling bias. Moreover, all non-admixed fish were late-spawning (January,February) whereas the majority of all trout caught for the study were ripe by November,December. The difference in spawning time could be an important factor delaying complete admixture of domesticated and indigenous trout. 5Synthesis and applications. This study demonstrates the feasibility of restoring populations that have been admixed with exogenous individuals, by identifying non-admixed individuals using genetic markers. However, the results also highlight the problem that numbers of identified non-admixed individuals may be small, necessitating identification of nearby, closely related populations that can be incorporated into breeding programmes. [source] Identification of microsatellite DNA markers for population structure analysis in Indian major carp, Cirrhinus mrigala (Hamilton-Buchanan, 1882)JOURNAL OF APPLIED ICHTHYOLOGY, Issue 2 2004K. K. Lal Summary Forty-four primer sequences available for four cyprinid fishes were tested to amplify microsatellite loci in Indian major carp, Cirrhinus mrigala. A total of 12 loci were successfully amplified with clear scorable patterns and five thereof were polymorphic. Suitability of the identified polymorphic loci in population structure analysis of C. mrigala was assessed. Genetic variation was examined in 76 specimens collected from five different rivers. The mean observed heterozygosity ranged from 0.247 to 0.333. Significant heterogeneity in allele frequencies was detected, indicating that the samples analysed did not belong to homogenous populations. The identified microsatellite markers are promising for the analysis of intraspecific divergence in C. mrigala across its distribution range. [source] Genetic Mapping of Magnaporthe grisea Avirulence Gene Corresponding to Leaf and Panicle Blast Resistant QTLs in Jao Hom Nin Rice CultivarJOURNAL OF PHYTOPATHOLOGY, Issue 6 2009Tanee Sreewongchai Abstract The avirulence characteristic of Magnaporthe grisea isolate TH16 corresponding to Jao Hom Nin (JHN) rice cultivar was studied by mapping population of 140 random ascospore progenies derived from the cross between B1-2 and TH16 isolates. Segregation analyses of the avirulence characteristic performing on JHN rice at the seedling and flowering stages were performed in this mapping population. We used the reference map of Guy11/2539 to choose microsatellite DNA markers for mapping the avirulence gene. The genetic map of this population was constructed from 39-microsatellite markers. The genetic map was spanned by covering seven chromosomes with an average distance of 11.9 cM per marker. In mapping population the distribution of pathogenic and non-pathogenic progenies on JHN rice were found to be fitted to 1 : 1 ratio for two of the rice stages, seedling and flowering stages. The Quantitative Trait Loci (QTL) analysis for avirulence genes corresponding to two rice stages were located at the same region on chromosome 2 between markers Pyms305 and Pyms435. The LOD score and percentage of phenotypic variance explained (PVE) on two rice stages were 5.01/16.69 and 6.73/20.26, respectively. These loci were designated as Avr-JHN(lb) and Avr-JHN(pb) corresponding to leaf and panicle blast characteristics. The findings of this study can be the initial step for positional cloning and identifying any function of avirulence genes corresponding to leaf and panicle blast characteristics. [source] Mitochondrial and microsatellite DNA markers reveal a Balkan origin for the highly invasive horse-chestnut leaf miner Cameraria ohridella (Lepidoptera, Gracillariidae)MOLECULAR ECOLOGY, Issue 16 2009R. VALADE Abstract Biological invasions usually start with a small number of founder individuals. These founders are likely to represent a small fraction of the total genetic diversity found in the source population. Our study set out to trace genetically the geographical origin of the horse-chestnut leafminer, Cameraria ohridella, an invasive microlepidopteran whose area of origin is still unkown. Since its discovery in Macedonia 25 years ago, this insect has experienced an explosive westward range expansion, progressively colonizing all of Central and Western Europe. We used cytochrome oxidase I sequences (DNA barcode fragment) and a set of six polymorphic microsatellites to assess the genetic variability of C. ohridella populations, and to test the hypothesis that C. ohridella derives from the southern Balkans (Albania, Macedonia and Greece). Analysis of mtDNA of 486 individuals from 88 localities allowed us to identify 25 geographically structured haplotypes. In addition, 480 individuals from 16 populations from Europe and the southern Balkans were genotyped for 6 polymorphic microsatellite loci. High haplotype diversity and low measures of nucleotide diversities including a significantly negative Tajima's D indicate that C. ohridella has experienced rapid population expansion during its dispersal across Europe. Both mtDNA and microsatellites show a reduction in genetic diversity of C. ohridella populations sampled from artificial habitats (e.g. planted trees in public parks, gardens, along roads in urban or sub-urban areas) across Europe compared with C. ohridella sampled in natural stands of horse-chestnuts in the southern Balkans. These findings suggest that European populations of C. ohridella may indeed derive from the southern Balkans. [source] Multiple paternity in a natural population of a wild tobacco fly, Bactrocera cacuminata (Diptera: Tephritidae), assessed by microsatellite DNA markersMOLECULAR ECOLOGY, Issue 11 2007SIMON D. SONG Abstract Mating frequency has important implications for patterns of sexual selection and sexual conflict and hence for issues such as speciation and the maintenance of genetic diversity. Knowledge of natural mating patterns can also lead to more effective control of pest tephritid species, in which suppression programmes, such as the sterile insect technique (SIT) are employed. Multiple mating by females may compromise the success of SIT. We investigated the level of polyandry and sperm utilization in a Brisbane field population of the tropical fruit fly, Bactrocera cacuminata (Hering), using seven polymorphic microsatellite loci. The offspring of 22 wild-caught gravid females were genotyped to determine the number of males siring each brood and paternity skew, using the programs gerud and scare. Our data showed that 22.7% of females produced offspring sired by at least two males. The mean number of mates per female was 1.72. Paternal contributions of double-sired broods were skewed with the most successful male having sired between 76.9% and 87.5% of the offspring. These results have implications for SIT, because the level of remating we have identified would indicate that wild females could mate with one or more resident fertile males. [source] Nuclear DNA analyses in genetic studies of populations: practice, problems and prospectsMOLECULAR ECOLOGY, Issue 3 2003De-Xing Zhang Abstract Population-genetic studies have been remarkably productive and successful in the last decade following the invention of PCR technology and the introduction of mitochondrial and microsatellite DNA markers. While mitochondrial DNA has proven powerful for genealogical and evolutionary studies of animal populations, and microsatellite sequences are the most revealing DNA markers available so far for inferring population structure and dynamics, they both have important and unavoidable limitations. To obtain a fuller picture of the history and evolutionary potential of populations, genealogical data from nuclear loci are essential, and the inclusion of other nuclear markers, i.e. single copy nuclear polymorphic (scnp) sequences, is clearly needed. Four major uncertainties for nuclear DNA analyses of populations have been facing us, i.e. the availability of scnp markers for carrying out such analysis, technical laboratory hurdles for resolving haplotypes, difficulty in data analysis because of recombination, low divergence levels and intraspecific multifurcation evolution, and the utility of scnp markers for addressing population-genetic questions. In this review, we discuss the availability of highly polymorphic single copy DNA in the nuclear genome, describe patterns and rate of evolution of nuclear sequences, summarize past empirical and theoretical efforts to recover and analyse data from scnp markers, and examine the difficulties, challenges and opportunities faced in such studies. We show that although challenges still exist, the above-mentioned obstacles are now being removed. Recent advances in technology and increases in statistical power provide the prospect of nuclear DNA analyses becoming routine practice, allowing allele-discriminating characterization of scnp loci and microsatellite loci. This certainly will increase our ability to address more complex questions, and thereby the sophistication of genetic analyses of populations. [source] Microsatellite analysis of female mating behaviour in lek-breeding sage grouseMOLECULAR ECOLOGY, Issue 8 2001K. Semple Abstract We used microsatellite DNA markers to genotype chicks in 10 broods of lek-breeding sage grouse, Centrocercus urophasianus, whose mothers' behaviour was studied by radio-tracking and observing leks. Previous behavioural studies suggested that almost all matings are performed by territorial males on leks and that multiple mating is rare. Two broods (20%) were sired by more than one male. Genetic analyses of the broods of eight females that visited an intensively studied lek were consistent with behavioural observations. Four females observed mating produced singly sired broods and males other than the individual observed copulating were excluded as sires for most or all of their chicks. Territorial males at the study lek were excluded as sires of broods of four other females that visited the lek but were not observed mating there. Radio-tracking suggested that two of these females mated at other leks. Our results confirm the reliability of mating observations at leks, but do not rule out a possible unseen component of the mating system. [source] Polymorphic microsatellite DNA markers in the Asiatic black bear Ursus thibetanusMOLECULAR ECOLOGY, Issue 10 2000E. Kitahara [source] Polymorphic microsatellite DNA markers for the marine gastropod Littorina subrotundataMOLECULAR ECOLOGY, Issue 1 2000A. D. Tie [source] Isolation and characterization of microsatellite loci in the whale shark (Rhincodon typus)MOLECULAR ECOLOGY RESOURCES, Issue 3 2009DENÍ RAMÍREZ-MACÍAS Abstract In preparation for a study on population structure of the whale shark (Rhincodon typus), nine species-specific polymorphic microsatellite DNA markers were developed. An initial screening of 50 individuals from Holbox Island, Mexico found all nine loci to be polymorphic, with two to 17 alleles observed per locus. Observed and expected heterozygosity per locus ranged from 0.200 to 0.826 and from 0.213 to 0.857, respectively. Neither statistically significant deviations from Hardy,Weinberg expectations nor statistically significant linkage disequilibrium between loci were observed. These microsatellite loci appear suitable for examining population structure, kinship assessment and other applications. [source] Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigasMOLECULAR ECOLOGY RESOURCES, Issue 3 2009C. SAUVAGE Abstract We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance. [source] Microsatellite DNA markers for three toad-headed lizard species (Phrynocephalus vlangalii, P. przewalskii and P. guttatus)MOLECULAR ECOLOGY RESOURCES, Issue 2 2009AIBIN ZHAN Abstract To assess the impact of natural landscapes on the population structure of lizards, 10 polymorphic microsatellite DNA markers were developed for the Qinghai toad-headed lizard, Phrynocephalus vlangalii. The number of alleles at these informative loci ranged from four to 28. The novel markers and those previously developed for Phrynocephalus przewalskii were cross-tested among three toad-headed lizard species P. vlangalii, P. przewalskii and P. guttatus. A high cross-utility rate of more than 58% was observed among these three species. These markers are expected to be useful tools for taxonomic considerations as well as population genetic analysis and future conservation management. [source] Development of 18 polymorphic microsatellite DNA markers of Laminaria japonica (Phaeophyceae)MOLECULAR ECOLOGY RESOURCES, Issue 4 2007YUANYUAN SHI Abstract Eighteen polymorphic microsatellite DNA markers were developed for Laminaria japonica, a brown alga cultured intensively in China. These markers are independent from each other and are moderately variable in L. japonica. The number of alleles and gene diversity detected in 53 gametophyte clones representing the varieties of L. japonica cultured once or currently in China range from two to nine and from 0.355 to 0.768, respectively. These markers will certainly facilitate the management and exploitation of the germplasm resource of L. japonica conserved indoor as gametophyte clones and the determination of the genetic diversity of L. japonica naturally distributed. [source] Isolation and characterization of polymorphic microsatellite DNA markers in the brown sole, Pleuronectes herzensteiniMOLECULAR ECOLOGY RESOURCES, Issue 1 2007S. G. KIM Abstract New microsatellite DNA markers from brown sole were developed and characterized. Fourteen primer sets were designed from 40 microsatellite regions. Eight of 14 loci exhibited variations comprising 8,31 alleles. Observed and expected heterozygosities ranged from 0.611 to 0.833 and from 0.647 to 0.968 among 36 individuals, respectively. Phz3, Phz8 and Phz12 significantly deviated from Hardy,Weinberg equilibrium, and there was a significant linkage disequilibrium between Phz2 and Phz12. Seven of eight loci conformed to the Mendelian manner of inheritance in a full-sib family. Seven to four loci of three related species were cross-amplified by primers for brown sole. [source] | |||||||||||||||||||||||||