Home About us Contact
|
Home About us Contact | ||
|
|
||
Microorganisms Isolated (microorganism + isolated)
Selected AbstractsCytokine production in response to endodontic infection in germ-free miceMOLECULAR ORAL MICROBIOLOGY, Issue 6 2002A. P. Ribeiro Sobrinho This study evaluated the cytokine profiles (type 1 or type 2) that are triggered by and modulate endodontic periapical infections in the root canal system of germ-free mice. Microorganisms isolated from two patients with pulpal necrosis were inoculated into two groups of experimental animals: group I (Gemella morbillorum) and group II (Bifidobacterium adolescentis, Fusobacterium nucleatum and Clostridium butyricum). In vitro, G. morbillorum induced type 1 cytokine synthesis, while the modulation processed in vivo seemed to have the opposite effect, with a reduction in the basal levels of IL-12 and IFN-,, IL-4-independent down-modulation. In vitro, microorganisms from group II, in poly-infection, induced a reduction of type 1 cytokine levels from day 10 to day 20, which seemed to be modulated via IL-4. In vivo, however, a predominance of the immune response to one species over the others occurred. [source] Oral manifestation of chronic mucocutaneous candidiasis: seven case reportsJOURNAL OF ORAL PATHOLOGY & MEDICINE, Issue 9 2007Xiaosong Liu Background:, Chronic mucocutaneous candidiasis (CMC) is a rare disorder characterized by persistent or recurrent candidal infections of the skin, nails and mucous membranes or by a variable combination of endocrine failure as well as immunodeficiency. Oral clinicopathological features of CMC have seldom been described in detail. Methods:, Seven patients with CMC were reported in the study. The clinical and histological findings, etiological Candida species, immunological evaluation, and therapeutic pattern of oral lesions, were analyzed. Results:, Long-standing whitish hyperplastic and nodule-like lesions with exaggerated deep fissure were the typical and characteristic oral manifestations presented by all patients. The tongue was the most common site affected. Histologically, no obvious distinction was found between CMC and other forms of candidal infection. Abnormal proportions of T-lymphocyte subsets and positive titers of autoantibody were observed in three subjects (42.9%) and one patient (14.3%) respectively. Meanwhile, four subjects (57.1%) showed decreased albumin and increased globulin, three cases (42.9%) had high levels of ESR. But no iron deficiency was found. Candida albicans was the microorganism isolated from these patients. Conclusions:, Multiple and widespread candidal infectious lesions can be observed on the oral cavity of CMC patients. Hyperplastic and nodule-like lesion with irremovable whitish patches and deep fissure are the most common oral manifestations of these patients. Dentists, otolaryngologists and pediatricians should be familiar with the clinical appearances of CMC to make an accurate diagnosis. Potential systemic disorders should be concerned to avoid the reoccurrence of oral candidiasis. [source] Growth kinetics of microorganisms isolated from Alaskan soil and permafrost in solid media frozen down to ,35°CFEMS MICROBIOLOGY ECOLOGY, Issue 2 2007Nicolai S. Panikov Abstract We developed a procedure to culture microorganisms below freezing point on solid media (cellulose powder or plastic film) with ethanol as the sole carbon source without using artificial antifreezes. Enrichment from soil and permafrost obtained on such frozen solid media contained mainly fungi, and further purification resulted in isolation of basidiomycetous yeasts of the genera Mrakia and Leucosporidium as well as ascomycetous fungi of the genus Geomyces. Contrary to solid frozen media, the enrichment of liquid nutrient solutions at 0°C or supercooled solutions stabilized by glycerol at ,1 to ,5°C led to the isolation of bacteria representing the genera Polaromonas, Pseudomonas and Arthrobacter. The growth of fungi on ethanol,microcrystalline cellulose media at ,8°C was exponential with generation times of 4.6,34 days, while bacteria displayed a linear or progressively declining curvilinear dynamic. At ,17 to ,0°C the growth of isolates and entire soil community on 14C-ethanol was continuous and characterized by yields of 0.27,0.52 g cell C (g of C-substrate),1, similar to growth above the freezing point. The ,state of maintenance,' implying measurable catabolic activity of non-growing cells, was not confirmed. Below ,18 to ,35°C, the isolated organisms were able to grow only transiently for 3 weeks after cooling with measurable respiratory and biosynthetic (14CO2 uptake) activity. Then metabolic activity declined to zero, and microorganisms entered a state of reversible dormancy. [source] Interactions of microorganisms isolated from gilthead sea bream, Sparus aurata L., on Vibrio harveyi, a pathogen of farmed Senegalese sole, Solea senegalensis (Kaup)JOURNAL OF FISH DISEASES, Issue 9 2005M Chabrillón Abstract Four bacterial isolates from farmed gilthead sea bream, Sparus aurata, included in a previous study as members of the Vibrionaceae and Pseudomonodaceae and the genus Micrococcus, have been evaluated for their adhesive ability to skin and intestinal mucus of farmed Senegalese sole, Solea senegalensis, and their antagonistic effect on Vibrio harveyi, a pathogen of sole. These isolates showed higher adhesion to sole mucus than the pathogenic strains of V. harveyi assayed. Only two of the isolates showed antagonistic activity to V. harveyi. Interactions of the four isolates with V. harveyi in respect of adhesion to skin and intestinal mucus under exclusion, competition and displacement conditions were studied. Three isolates were able to reduce the attachment to skin and intestinal sole mucus of a pathogenic strain of V. harveyi under displacement and exclusion conditions, but not under competition conditions. The in vivo probiotic potential of isolate Pdp11 was assessed by oral administration followed by challenge with the pathogenic V. harveyi strain Lg14/00. A group of 50 Senegalese sole received a commercial diet supplemented with 108 cfu g,1 of lyophilized Lg14/00 for 15 days. A second group of fish received a non-supplemented commercial diet. After challenge the mortality of the fish receiving the diet supplemented with the potential probiotic isolate was significantly lower than that in the fish receiving the non-supplemented commercial diet. This study has shown that the ability to interfere with attachment of pathogens, as well as the adhesion to host surfaces, are suitable criteria for selection of candidate probiotics for use in the culture of Senegalese sole. [source] Microbial contamination of contact lenses and lens care accessories of soft contact lens wearers (university students) in Hong KongOPHTHALMIC AND PHYSIOLOGICAL OPTICS, Issue 1 2007M. S. Yung Abstract Purpose:, This study aimed to examine the rates of microbial contamination, and identify contaminants associated with contact lenses and lens care accessories used by a group of young contact lens wearers. Methods:, Collected contact lenses, lens cases, and lens care solutions were studied by bacterial culture. Contamination rates of these samples were recorded and compared with those reported in previous studies. Results:, Of the samples tested, 9% of lens extracts, 34% of case extracts and 11% of solution samples were contaminated with ocular pathogenic microorganisms. Serratia spp., Staphylococcus aureus and coagulase-negative staphylococci were the most common microorganisms isolated. Lens cases were the most frequently contaminated item. Lens cases also yielded the widest range of bacterial isolates. Contact lenses used by occasional wearers were associated with a higher contamination rate. Using either saline or multipurpose solution to rinse lenses before use appeared to be effective in reducing incidence of contamination. Conclusion:, Our findings demonstrate that contact lenses and lens care accessories are not well maintained by contact lens wearers. Regular reviews and reinforcement of lens care procedures for the usage and care of contact lenses and lens care accessories is therefore important and essential. [source] Bacteria commonly isolated from keratitis specimens retain antibiotic susceptibility to fluoroquinolones and gentamicin plus cephalothinCLINICAL & EXPERIMENTAL OPHTHALMOLOGY, Issue 1 2006Cameron N Ly MB BS Abstract Purpose:, Patients presenting with presumed infective keratitis were studied to determine predisposing factors, the current susceptibilities of the bacterial isolates to a range of relevant antibiotics, the success rate of topical antibiotic treatment of keratitis and predictors of failure of topical therapy. Methods:, Corneal scrapings taken from patients who presented between January 2002 and December 2003 to the Sydney Eye Hospital Emergency Department with keratitis were cultured. The minimum inhibitory concentration of selected antibiotics was determined for each bacterial isolate using an agar dilution technique. Results:, One hundred and twelve consecutive patients presented with corneal ulcers. Forty-seven of the 112 (42%) patients had a growth from the corneal scraping. Potential predisposing factors were identified in 64% of patients, most frequently contact lens wear (36% of patients). Coagulase-negative staphylococci were the most common species isolated. Other common organisms isolated include Pseudomonas aeruginosa, Corynebacterium spp., Staphylococcus aureus and Streptococcus spp. Conclusions:, Most microorganisms isolated from patients with bacterial keratitis showed susceptibility to ciprofloxacin and aminoglycosides. Cephalothin plus aminoglycoside constituted an effective initial broad-spectrum antibiotic combination. The success rate of topical antibiotic treatment of corneal abscess is 89%. Predictors of failure include older age group, medium or large ulcer, culture-negative keratitis, hypopyon and poor visual acuity. [source] | ||||||||||