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Microbial Profiles (microbial + profile)

Distribution by Scientific Domains
Medical Sciences81%

Kinds of Microbial Profiles

  • subgingival microbial profile
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    Selected Abstracts

    Biofilms in the Edentulous Oral Cavity

    JOURNAL OF PROSTHODONTICS, Issue 5 2008
    Amit Sachdeo BDS, DMSc
    Abstract Purpose: The oral cavity presents numerous surfaces for microbial colonization. These surfaces produce biofilms of differing complexities unique to each individual. Several studies have looked at biofilms in dentate patients. There has been limited research regarding biofilms on dentures or soft tissues of edentulous patients. The purpose of the present investigation was to provide meaningful data describing microbial ecological relationships in the oral cavity of edentulous patients and to evaluate the microbiota on hard and soft tissue surfaces and saliva in edentulous patients wearing complete dentures. Materials and Methods: Sixty-one edentulous subjects with complete maxillary and mandibular dentures were recruited. "Supragingival" biofilm samples were taken from 28 denture teeth for each subject. Biofilm samples were also taken from the dorsal, lateral, and ventral surfaces of the tongue, floor of mouth, buccal mucosa, hard palate, vestibule/lip, "attached gingiva," and saliva. Samples were individually analyzed for their content of 41 bacterial species using checkerboard DNA,DNA hybridization. Levels and proportions of each species were determined for every sample location. Results: Periodontal pathogens such as Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis were clearly present in the samples from the edentulous subjects. Microbial profiles in samples from the soft tissue surfaces differed among site locations. Samples from the dorsum of the tongue exhibited the highest bacterial counts followed by the "attached gingiva" and the lateral surfaces of the tongue, while the lowest mean counts were found in samples from the buccal mucosa and labial vestibules. Using cluster analysis of the proportions of the test species, three clusters were formed. The first cluster comprised saliva, supragingival plaque, and the lateral and dorsal surfaces of the tongue. The second cluster comprised the other six soft tissue surfaces. Species on the denture palate formed a third cluster. Conclusions: One of the major findings in this study was the detection of periodontal pathogens, A. actinomycetemcomitans and P. gingivalis, in the edentulous subjects, as these species were thought to disappear after removal of all natural teeth. This finding has implications regarding future dental treatment and the general health of individuals. Distinct patterns of microbial colonization were seen on the different soft tissue surfaces. Thus, this investigation provided the first step in defining the organisms that are associated with edentulous patients on both soft (mucosa) and hard surfaces (denture). The study also provided meaningful data that described microbial ecological relationships in the oral cavity of edentulous subjects. The authors believe that this study is the first comprehensive assessment of the microbiota in the complete denture-wearing subject. [source]

    Use of a lactic acid bacteria starter culture during green olive (Olea europaea L cv Ascolana tenera) processing

    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 7 2005
    Vincenzo Marsilio
    Abstract Among the Italian olive germplasm, ,Ascolana tenera' is one of the best varieties for table olive production. This research addressed the impact of different processing types (Greek-style and Spanish-style) on the fermentation and phenolic composition of olive fruit. In particular, the effects of a lactic acid bacteria (LAB) starter culture on the fermentation of naturally green olives processed according to the traditional Greek method were studied. Results revealed that Spanish-style processing produced a dramatic loss of total phenolics, while natural olive processing favoured a higher retention of biophenols. Oleoside 11-methylester, a phenol-related compound, and hydroxytyrosol, tyrosol, vanillic acid, 3,4-dihydroxyphenylglycol, oleuropein and oleuropein aglycons, as the main phenols, were detected in olive fruit. More interestingly, this research indicated that inoculation with LAB affected the pH, total acidity, microbial profile and palatability of olives. Olives fermented with the LAB starter culture were perceived by panellists to be less bitter and more aromatic than those spontaneously fermented. Thus the use of LAB inoculants during olive fermentation could be applied with the currently available technology. Copyright © 2005 Society of Chemical Industry [source]

    Effect of khat chewing on 14 selected periodontal bacteria in sub- and supragingival plaque of a young male population

    MOLECULAR ORAL MICROBIOLOGY, Issue 3 2005
    N. N. Al-Hebshi
    Background/aims:, The habit of chewing khat (Catha edulis) for its amphetamine-like effects is highly prevalent in Yemen and east Africa, and has expanded to Western countries. The purpose of this study was to estimate and compare the prevalence and levels of 14 periodontal bacteria in gingival plaque of khat chewers and khat nonchewers, as well as of khat chewing sides and khat nonchewing sides. Methods:, A total of 408 sub- and supragingival plaque samples were collected from 51 young males (29 khat chewers and 22 khat nonchewers; age range 19,28 years) and analyzed using whole genomic DNA probes and checkerboard DNA,DNA hybridization. Clinical parameters were recorded for all teeth at six sites per tooth. Results:,Streptococcus intermedius and Veillonella parvula were significantly more prevalent in the subgingival plaque of chewers, which also showed significantly higher levels of V. parvula and Eikenella corrodens. Similar results were found for the subgingival plaque of the chewing sides compared to the nonchewing sides. However, there was a significantly higher prevalence and higher levels of Tannerella forsythia in the subgingival plaque of the nonchewing sides. No significant differences were observed for the supragingival plaque between the two study groups. There was a significantly lower prevalence of Capnocytophaga gingivalis and Fusobacterium nucleatum in the khat chewing sides, and higher levels of V. parvula and Actinomyces israelii. Conclusion:, The data suggest that khat chewing induces a microbial profile that is not incompatible with gingival health. [source]

    Antibiotic susceptibility of urogenital microbial profile of infertile men in South-eastern Nigeria

    ANDROLOGIA, Issue 4 2010
    C. J. Uneke
    Summary Male factors are known to contribute significantly to the infertility problem. The urogenital bacteria profile and semen quality were investigated among 160 men attending infertility clinics in South-eastern Nigeria. Both semen and urine samples were obtained from each subject and analysed according to standard techniques. A total of 16 (10%) had bacterial infection in their semen samples and individuals of older age groups were significantly affected (,2 = 23.18, P < 0.05). Urinary tract infection (UTI) was observed in 30.6% of subjects, with the highest rate occurring among men aged 36,40 years, but the difference was not significant (,2 = 7.62, P > 0.05). A total of 33.8% of the men had semen volume less than 2 ml per ejaculate, 33.8% also recorded total sperm count of <40 × 106 ml,1, while 36.3% recorded sperm motility <50%. Fifty-three individuals (33.1%) had suboptimal sperm morphology (<60%), with the men aged 46,50 years old significantly affected (,2 = 13.03, P < 0.05). All the individuals with bacterial infection in semen also had UTI and suboptimal semen parameters. The commonest bacterial isolates were Proteus species, Staphylococcus aureus and Escherichia coli, which were resistant to most of the antibiotics assessed. [source]

    Periodontal microbiota and clinical periodontal status in a rural sample in southern Thailand

    EUROPEAN JOURNAL OF ORAL SCIENCES, Issue 5 2002
    P. N. Papapanou
    We sought to determine (i) the association of subgingival bacterial profiles to clinical periodontal status in a population with limited access to dental care in Thailand, and (ii) the external validity of our earlier findings from a similar study in rural China. We examined 356 subjects, 30,39 yr old and 50,59 yr old, with respect to clinical periodontal status and subgingival plaque at maximally 14 sites per subject. Checkerboard hybridizations were used to analyse a total of 4343 samples. The prevalence of the 27 species investigated ranged between 87.2% and 100%. Discriminant analysis based on microbial profiles classified correctly 67.5% of all deep (, 5 mm) and 64.2% of all shallow sites, and 67.4% of all subjects with and 69.3% of all subjects without , 3 deep pockets. High colonization by ,red complex' bacteria was four times as likely (95% Confidence Limits (CL) 2.5,6.6) in subjects with ,,10 sites with attachment loss of ,,5 mm, and 4.3 times as likely (95% CL 2.6,7.1) in subjects with , 30 such sites. The data confirmed (i) the ubiquitous prevalence of the bacteria investigated in subjects with no regular access to dental care; and (ii) the high odds for periodontal pathology conferred by increased levels of specific periodontal bacteria. [source]

    Linkage of microbial ecology to phenotype: correlation of rumen microbial ecology to cattle's feed efficiency

    FEMS MICROBIOLOGY LETTERS, Issue 1 2008
    Le Luo Guan
    Abstract Linkage of rumen microbial structure to host phenotypical traits may enhance the understanding of host,microbial interactions in livestock species. This study used culture-independent PCR-denaturing gradient gel electrophoresis (PCR-DGGE) to investigate the microbial profiles in the rumen of cattle differing in feed efficiency. The analysis of detectable bacterial PCR-DGGE profiles showed that the profiles generated from efficient steers clustered together and were clearly separated from those obtained from inefficient steers, indicating that specific bacterial groups may only inhabit in efficient steers. In addition, the bacterial profiles were more likely clustered within a certain breed, suggesting that host genetics may play an important role in rumen microbial structure. The correlations between the concentrations of volatile fatty acids and feed efficiency traits were also observed. Significantly higher concentrations of butyrate (P<0.001) and valerate (P=0.006) were detected in the efficient steers. Our results revealed potential associations between the detectable rumen microbiota and its fermentation parameters with the feed efficiency of cattle. [source]

    Subgingival microbial profiles in chronic periodontitis patients from Chile, Colombia and Spain

    JOURNAL OF CLINICAL PERIODONTOLOGY, Issue 2 2008
    David Herrera
    Abstract Aim: To investigate the subgingival microbiota of distinct periodontitis patient populations, in Chile, Colombia and Spain, using identical clinical and bacteriological methods. Material and Methods: In this multicentre study, 114 chronic periodontitis patients were selected. Patients were examined using an identical clinical protocol and pooled subgingival samples were obtained from each patient. Samples were processed in the three laboratories by means of culturing under identical clinical and microbiological protocols. Total anaerobic counts and frequency of detection and proportions of nine periodontal pathogens were calculated. Variables were analysed by means of anova, ,2, Kruskal,Wallis and Dunn's multiple comparison tests. Results: The Colombian population demonstrated greater severity of periodontitis, with significantly deeper mean probing pocket depth, and had a significantly lower percentage of current smokers. When comparing samples from the three patient populations, the total counts were significantly higher in the Colombian patients. The numbers of putative pathogens differed among groups. Tannerella forsythia was found less frequently in Chilean samples, while Parvimonas micra and enteric rods differed significantly among the three population groups. Conclusion: Significant differences among Chile, Colombia and Spain existed regarding the frequency and proportions of specific periodontal pathogens in the subgingival microbiota of periodontitis patients. [source]

    Change in subgingival microbial profiles in adult periodontitis subjects receiving either systemically-administered amoxicillin or metronidazole

    JOURNAL OF CLINICAL PERIODONTOLOGY, Issue 7 2001
    M. Feres
    Abstract Aim: The current investigation evaluated changes in levels and proportions of 40 bacterial species in subgingival plaque samples during, immediately after and up to 1 year after metronidazole or amoxicillin therapy combined with SRP. Method: After baseline clinical and microbiological monitoring, 17 adult periodontitis subjects received full mouth SRP and 14 days systemic administration of either metronidazole (250 mg, TID, n=8) or amoxicillin (500 mg, TID, n=9). Clinical measurements including % of sites with plaque, gingival redness, bleeding on probing and suppuration, pocket depth (PD) and attachment level (AL) were made at baseline, 90, 180 and 360 days. Subgingival plaque samples were taken from the mesial surface of all teeth in each subject at baseline, 90, 180 and 360 days and from 2 randomly selected posterior teeth at 3, 7, and 14 days during and after antibiotic administration. Counts of 40 subgingival species were determined using checkerboard DNA-DNA hybridization. Significance of differences over time was determined using the Quade test and between groups using ANCOVA. Results: Mean PD was reduced from 3.22±0.12 at baseline to 2.81±0.16 (p<0.01) at 360 days and from 3.38±0.23 mm to 2.80±0.14 mm (p<0.01) in the amoxicillin and metronidazole treated subjects respectively. Corresponding values for mean AL were 3.21±0.30 to 2.76±0.32 (p<0.05) and 3.23±0.28 mm to 2.94±0.23 mm (p<0.01). Levels and proportions of Bacteroides forsythus, Porphyromonas gingivalis and Treponema denticola were markedly reduced during antibiotic administration and were lower than baseline levels at 360 days. Counts (×105, ±SEM) of B. forsythus fell from baseline levels of 0.66±0.16 to 0.04±0.02, 0.13±0.04, 0.10±0.03 and 0.42±0.19 in the amoxicillin group at 14, 90, 180 and 360 days respectively (p<0.001). Corresponding values for metronidazole treated subjects were: 1.69±0.28 to 0.02±0.01, 0.20±0.08, 0.22±0.06 and 0.22±0.08 (p<0.001). Counts of Campylobacter species, Eubacterium nodatum, Fusobacterium nucleatum subspecies, F. periodonticum and Prevotella nigrescens were also detected at lower mean levels during and immediately after therapy, but gradually increased after withdrawal of the antibiotics. Members of the genera Actinomyces, Streptococcus and Capnocytophaga were minimally affected by metronidazole. However, amoxicillin decreased the counts and proportions of Actinomyces species during and after therapy. Conclusions: The data suggest that metronidazole and amoxicillin are useful in rapidly lowering counts of putative periodontal pathogens, but must be accompanied by other procedures to bring about periodontal stability. Zusammenfassung Ziel: Die gegenwärtige Untersuchung evaluiert die Veränderungen in den Niveaus und Proportionen von 40 bakteriellen Spezies in subgingivalen Plaqueproben während, sofort nach und bis zu 1 Jahr nach Metronidazol- oder Amoxicillintherapie in Kombination mit SRP. Methoden: Nach der klinischen und mikrobiologischen Basisuntersuchung erhielten 17 erwachsene Personen mit Parodontitis eine vollständige SRP und 14 Tage eine systemische Gabe von entweder Metronidazol (250 mg, TID, n=8) oder Amoxicillin (500 mg, TID, n=9). Die klinischen Messungen schlossen die Prozentwerte der Flächen mit Plaque, der gingivalen Rötung, der Provokationsblutung und Suppuration, der Sondierungstiefe (PD) und des Stützgewebeniveaus (AL) ein. Die Messungen wurden zur Basis, am 90., am 180. und 360. Tag gemacht. Die subgingivalen Plaqueproben wurden von der mesialen Oberfläche aller Zähne zur Basis, zum 90., zum 180. und 360. Tag von jedem Probanden genommen sowie von 2 zufällig ausgesuchten posterioren Zähnen am Tag 3, 7 und 14 während und nach der Antibiotikaverordnung. Die Mengen von 40 subgingivalen Spezies wurden unter Nutzung einer checkerboard DNA-DNA Hybridisation bestimmt. Die Signifikanzen der Differenzen über die Zeit wurden mit dem Quade-Test und zwischen den Gruppen mit der ANCOVA überprüft. Ergebnisse: Die mittleren PD reduzierten sich von 3.22±0.12 mm zur Basis zu 2.81±0.16 mm (p<0.01) zum 360. Tag und von 3.38±0.23 mm zu 2.80±0.14 mm (p<0.01) bei den mit Amoxicillin bzw. mit Metronidazol behandelten Patienten. Korrespondierende Werte für die mittleren AL waren 3.21±0.30 zu 2.76±0.32 (p<0.05) und 3.23±0.28 mm zu 2.94±0.23 mm (p<0.01). Die Niveaus und die Verteilung von Bacteroides forsythus, Porphyromonas gingivalis und Treponema denticola wurden während der Antibiotikabehandlung deutlich reduziert und waren am 360. Tag niedriger als zur Basis. Die Mengen (×105, ±SEM) von B. forsythus fielen von der Basis von 0.66±0.16 auf 0.04±0.02, 0.13±0.04, 0.10±0.03 und 0.42±0.19 in der Amoxicillin Gruppe an den Tagen 14, 90, 180 und 360 (p<0.001). Korrespondierende Werte für die mit Metronidazol behandelten Personen waren: 1.69±0.28 zu 0.02±0.01, 0.20±0.08, 0.22±0.06 und 0.22±0.08 (p<0.001). Die Mengen von Campylobacter sp., Eubacterium nodatum, Fusobacterium nucleatum subspecies, F. peridonticum und Prevotella nigrescens waren in den mittleren Niveaus während und sofort nach der Therapie auch niedriger, aber graduell erhöht nach Absetzen der Antibiotika. Mitglieder der Klassen Actinomyces, Streptococcus und Capnocytophaga wurden durch Metronidazol minimal beeinflußt. Jedoch verringerte Amoxicillin die Mengen und Verhältnisse von Actinomyces sp. während und nach der Therapie. Zusammenfassung: Die Daten suggerieren, daß Metronidazol und Amoxicillin in der schnellen Verringerung der Mengen von putativen parodontalen Pathogenen nützlich sind, daß dies aber durch andere Prozeduren begleitet wurden muß, um parodontale Stabilität zu erbringen. Résumé But: La présente recherche a évalué les modifications de niveaux et de proportions de 40 espèces bactériennes dans des prélèvements de plaque sous gingivale pendant, immédiatement après, et jusqu'à un an après un traitement par métronidazole ou amoxicilline associè avec le détartrage/surfaçage radiculaire. Méthode: Après avoir relevé les paramètres cliniques et microbiologiques initiaux, 17 sujets atteints de parodontite de l'adulte ont subi un détartrage/surfaçage radiculaire de toute la bouche et l'administration systémique pendant 14 jours de métronidazole (250 mg, 3× fois par jour, n=8) ou d'amoxicilline (500 mg, 3× par jour, n=9). Les mesures cliniques relevées initialement, à 90 jours, à 180 jours, et à 360 jours, étaient: le % de sites avec de la plaque, la rougeur gingivale, le saignement au sondage et la suppuration, la profondeur de poche (PD) et le niveau d'attache (AL). Des échantillons de plaque sous gingivale étaient prélevés sur la surface mésiale de toutes les dents, chez chaque sujet, initialement, à 90 jours, à 180 jours, et á 360 jours, et sur 2 dents postérieures choisies au hasard à 3, 7, et 14 jours pendant et après l'administration d'antibiotique. Le comptage de 40 expèces sous gingivales fut déterminé par la technique de l'hybridisation en damier DNA-DNA. La signification des différences au cours du temps fut déterminée par le test de Quade et entre les groupes par ANCOVA. Résultats: La profondeur moyenne des poches a étê réduite de 3.22±0.12 mm initialement à 2.81±0.16 mm (p<0.01) à 360 jours et de 3.38±0.28 mm à 2.80±0.14 mm (p<0.01) dans les groupes amoxicilline et metronidazole, respectivement. Les valeurs correspondantes pour AL étaient 3.21±0.30 à 2.76±0.32 (p<0.05) et 3.23±0.28 à 2.94±0.23 (p<0.01). Les niveau de B. forsythus, P. gingivalis et T. denticola, étaient fortement réduits pendant l'administration d'antibiotique et restaient plus bas à 360 jours qu'initialement. Les comptages (×105, ±SEM) de B. forsythus tombaient de niveaux initiaux de 0.66±0.16 à 0.04±0.02, 0.13±0.04, 0.10±0.03 et 0.42±0.19 dans le groupe amoxicilline à 14 jours, 90 jours, 180 jours, et 360 jours, respectivement (p<0.001). Les valeurs correspondantes pour les sujets traits par métronidazole étaient de: 1.69±0.28 à 0.02±0.01, 0.20±0.08, 0.22±0.06 et 0.22±0.08 (p<0.001). Les comptages des espèces Camopylobacter, Eubacterium nodatum, des espèces Fusobacterium nodatum, F. periodonticum et Prevotella nigrescensétaient également détectés à des niveaux moyens plus bas pendant, et immédiatement après traitement, mais augmentaient graduellement après cessation des antibiotiques. Les membres des genres Actinomyces, Streptococcus et Capnocytophagaétaient très peu affectés par le métronidazole. Par contre, l'amoxicilline diminuait les comptage et les proportions des Actinomyces pendant et après le traitement. Conclusions: Ces données suggèrent que le métronidazole et l'amoxicilline sont utiles pour diminuer rapidement les comptages des pathogènes parodontaux putatifs, mais qu'ils doivent être accompagnés d'autres procédés pour apporter une stabilité parodontale. [source]

    Interleukin-1 gene polymorphism and periodontal status

    JOURNAL OF CLINICAL PERIODONTOLOGY, Issue 5 2001
    A case-control study
    Abstract Objectives: This case-control study examined polymorphisms at the interleukin-1 gene in relation to periodontal status, subgingival bacteria and systemic antibodies to periodontal microbiota. Methods: 132 periodontitis patients were age- and gender-matched with 73 periodontally intact controls. Full-mouth clinical assessments of the periodontal tissues were performed. Subgingival plaque samples (2440 in total) were analyzed by genomic DNA probes, and serum IgG antibodies to periodontal microbiota were assessed by an immunoassay. Polymorphisms in the IL-1A gene at position +4845 and the IL-1B gene at position +3953 were studied by PCR. A composite positive genotype was defined as at least one rare (#2) allele present at each locus. Results: No skewed distribution of the composite genotype was observed between cases and controls (45.2% vs 41.7%). In cases, both the composite genotype and the number of #2 alleles were positively correlated with the severity of attachment loss. No relationship between genotype and subgingival microbial profiles was observed. Genotype positive patients revealed both overall lower serum antibody levels and specific titers against selected bacteria. Conclusions: The composite genotype failed to distinguish between periodontitis patients and controls but correlated in patients with the severity of the disease and the antibody responses to periodontal microbiota. Zusammenfassung Grundlagen: Diese Fall-kontrollierte Studie prüfte die Polymorphismen am Interleukin-1 Gen in Beziehung zum parodontalen Status, subgingivalen Bakterien und systemischen Antikörpern zu parodontalen Mikroorganismen. Methoden: 132 Parodontitis-Patienten wurden nach Alter und Geschlecht mit 73 parodontal gesunden Kontrollen gemischt. Eine vollständige klinische Überprüfung des parodontalen Gewebes wurde durchgeführt. Subgingivale Plaqueproben (insgesamt 2440) wurden mit Genom DNA Testen analysiert, und die Serum IgG Antikörper zu parodontalen Bakterien wurden mit einem Immunoassay bestimmt. Die Polymorphismen am IL-1A Gen an den Stellen +4845 und am IL-1B Gen an der Positon +3953 wurden mittels PCR überprüft. Ein zusammengefaßter positiver Genotyp wurde so definiert, daß mindestens ein seltenes Allel (#2) an jeder Position vorhanden war. Ergebnisse: Es wurde keine schiefe Verteilung der zusammengefaßten Genotypen zwischen den Probanden und den Kontrollen (45.2% versus 41.7%) beobachtet. Bei den Probanden waren sowohl der zusammengefaßte Genotyp als auch die Anzahl der #2 Allele positiv mit dem Ausmaß des Stützgewebeverlustes korreliert. Zwischen den Genotypen und den subgingivalen Bakterienprofilen wurden keine Beziehungen gefunden. Genotyp positive Patienten zeigten sowohl allgemein niedrigere Serumantikörperlevel als auch spezifischen Titer gegen die selektierten Bakterien. Zusammenfassung: Der zusammengefaßte Genotyp untereschied sich nicht zwischen den Parodontitis-Patienten und den Kontrollen, aber korrelierte bei den Patienten mit der Schwere der Erkrankung und der Antikörperantwort auf parodontalen Bakterien. Résumé Cette étude a examiné les polymorphismes du gène Interleukine-1 (IL-1) en relation avec l'état parodontal, les bactéries sous-gingivales et les anticorps systémiques aux bactéries parodontales. 132 patients avec parodontite d'âge et de sexe similaires aux 73 contrôles sans problèmes parodontaux ont été recrutés. Les analyses clinique des tissus parodontaux de toute la bouche ont été effectuées. Des échantillons de plaque dentaire sous-gingivale (2220 au total) ont été analysés par des sondes ADN génomiques et des anticorps IgG sériques aux bactéries parodontales ont été analysés par immuno-essais. Les polymorphismes de IL-1A à la position +4845 et de IL-1B à la position +3953 ont étéétudiés par une réaction de la chaîne polymérase (PCR). Un génotype composite positif était défini comme un rare (#2) allèle présent à chaque endroit. Aucune répartition spéciale du génotype composite n'a été observée entre les cas et les contrôles (45.2% versus 42%). Chez les personnes présentant des cas tant le génotype composite que le nombre d'allèle #2 étaient en relation positive avec la sévérité de la perte d'attache. Aucune relation entre le génotype et les profils microbient sous-gingivaux n'a été mise en évidence. Les patients positifs aux génotypes possédaient des niveaux d'anticorps sériques et des titres spécifiques inférieurs contre des bactéries sélectionnées. Le génotype composite ne permet pas la distinction entre les patients avec parodontite et les contrôles, mais est en corrélation chez les patients avec la sévérité de la maladie et les résponses de l'anticorps à la flore parodontale. [source]

    The microbiota on different oral surfaces in healthy children

    MOLECULAR ORAL MICROBIOLOGY, Issue 3 2009
    W. Papaioannou
    Introduction:, Knowledge of the early oral colonization patterns could provide a better understanding of oral biofilm development and disease initiation that in turn could be the basis for early preventive programmes. Methods:, Microbial samples were collected from five different oral habitats from a total of 93 children (age 3,12 years), attending the Dental School of the University of Athens, who were split into three age groups. A total of 38 microbial species were sought out by the checkerboard DNA,DNA hybridization technique. Results:, All of the test species, except Parvimonas micra and Porphyromonas gingivalis, differed significantly among sample locations providing quite distinct microbial profiles for the different oral surfaces. Supragingival and subgingival plaque had similar profiles and exhibited higher proportions of Actinomyces species and Green complex while soft tissue samples were dominated by streptococci of the Yellow complex. The profiles of the tongue dorsum and saliva were also similar. Many of the species were in similar proportions in all three age groups for a given location. Periodontal pathogens showed increases in proportions with increasing age. Specifically, the Red complex species (Tannerella forsythia, P. gingivalis, Treponema denticola) showed a significant increase in proportion with age (P < 0.05) in all sample locations. Conclusions:, The results showed a pattern of colonization in children similar to that previously found in adults. Differences in the profile between age groups suggest a gradual maturation of the oral microbiota, with it being made up of an increasing number of Orange and Red complex species. [source]

    Relationship of Porphyromonas gingivalis with glycemic level in patients with type 2 diabetes following periodontal treatment

    MOLECULAR ORAL MICROBIOLOGY, Issue 4 2008
    N. Makiura
    Introduction:, The aim of this study was to assess the relationship between serum glycemic levels and subgingival microbial profile alteration following periodontal treatment in patients with type 2 diabetes mellitus. Methods:, We studied 30 periodontitis patients with type 2 diabetes mellitus who received full-mouth subgingival debridement by analyzing their subgingival microbial profiles using a polymerase chain reaction method at baseline and various time-points for 12 months following treatment. Concurrently, probing pocket depth, bleeding on probing, and metabolic parameters, including glycated hemoglobin A1c (HbA1c), blood sugar level, C-reactive proteins, total cholesterol, triglyceride, and high-density and low-density lipoprotein cholesterol, were recorded. Results:, Periodontal conditions were significantly improved after treatment, and the occurrence rates of periodontal bacterial species, including Porphyromonas gingivalis, Tannerella forsythensis, Treponema denticola, and Prevotella intermedia, were also reduced. Interestingly, P. gingivalis was detected more frequently in subjects with increased HbA1c values after periodontal treatment than in those patients with decreased HbA1c values. Furthermore, P. gingivalis with type II fimbriae was detected only in HbA1c-increased subjects, while improvements in HbA1c values were observed only in subjects without type II clones. Conclusions:, These results suggest that glycemic level in diabetes is affected by the persistence of P. gingivalis, especially clones with type II fimbriae, in periodontal pockets. [source]