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Microbial Phytase (microbial + phytase)

Distribution by Scientific Domains

Life Sciences	60%

Selected Abstracts

Influence of exogenous enzyme supplementation on energy utilisation and nutrient digestibility of cereals for broilers

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 14 2004
YB Wu
Abstract The influence of a microbial phytase, produced by solid-state fermentation, on the apparent metabolisable energy and ileal digestibility of phosphorus, nitrogen and starch in sorghum, maize, wheat and barley was examined using 4-week-old broilers. For wheat and barley, the influence of phytase, individually or in combination with glycanases, was also evaluated. Microbial phytase improved (p < 0.05) apparent ileal phosphorus digestibility in all cereals. Phytase supplementation improved (p < 0.05) the apparent metabolisable energy of maize and barley by 2.6 and 7.8%, respectively. Numerical improvements in apparent metabolisable energy were observed in sorghum (1.9%) and wheat (2.1%), but were not statistically significant (p > 0.05). Further improvements (p < 0.05) in the apparent metabolisable energy of wheat and barley were observed when the phytase was combined with glycanases. However, the observed improvements in apparent metabolisable energy were not always associated with enhanced ileal digestibility of protein and starch. Copyright © 2004 Society of Chemical Industry [source]

Dietary phytate (inositol hexaphosphate) regulates the activity of intestinal mucosa phytase

JOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 5 2009
E. M. Onyango
Summary The role of dietary phytate (inositol hexaphosphate) in the regulation of intestinal mucosa phytase was investigated in chicks. Seven-day-old chicks were grouped by weight into six blocks of three cages with six birds per cage. Three purified diets [a chemically defined casein diet, a chemically defined casein diet plus sodium phytate (20 g/kg diet) and a chemically defined casein diet plus sodium phytate (20 g/kg diet) and microbial phytase (1000 units/kg diet)] were randomly assigned to cages within each block. Chicks were fed experimental diets from 8 to 22 days of age then killed, and duodenal mucosa and left tibia removed. Phytase activity in duodenal mucosa, growth performance and bone ash content were determined. Addition of phytate to the chemically defined casein diet reduced (p < 0.05) the Vmax of the duodenal brush border phytase, but the Km of the enzyme was not affected. Addition of phytate also reduced (p < 0.05) weight gain, feed intake, feed efficiency and percentage ash. Addition of microbial phytase fully restored the feed efficiency (p < 0.05), but Vmax and body weight gain were only partially restored (p < 0.05). In conclusion, it would seem that dietary phytates non-competitively inhibit intestinal mucosa phytase. [source]

Influence of exogenous enzyme supplementation on energy utilisation and nutrient digestibility of cereals for broilers

Water-soluble phosphorus excretion in pigs fed diets supplemented with microbial phytase

ANIMAL SCIENCE JOURNAL, Issue 3 2009
Joshua A. JENDZA
ABSTRACT Three experiments were conducted to compare the excretion of water-soluble phosphorus (P) of starter, grower, and finisher pigs fed corn-soybean meal-based P-deficient basal diet containing no added inorganic P (B), P-adequate diet (the basal diet with added dicalcium phosphate; B + P), the basal diet plus 500 units of microbial phytase/kg (B + 500), or the basal diet plus 1000 units of microbial phytase/kg (B + 1000). There were 6 barrows per diet, in a randomized complete block design, with an average initial body weight of 10.4, 20.0, or 51.1 kg for each of starter, grower, and finisher pigs in the three phosphorus balance experiments, respectively. In the experiments, the addition of dicalcium phosphate or phytase to the basal diet increased (P < 0.05) the digestibility and retention of P, and there were linear reductions (P < 0.05) in water-soluble P excretion as a result of supplementing the basal diet with phytase. In the starter pig experiment, phytase addition at 500 or 1000 units/kg reduced (P < 0.05) water-soluble P excretion by 28 or 42%, respectively when compared with the B + P diet. In the grower pig experiment, adding phytase at 500 or 1000 units/kg reduced (P < 0.05) water-soluble P excretion by 24 or 34%, respectively when compared with the B + P diet. The use of phytase at 500 or 1000 units/kg reduced (P < 0.05) water-soluble P excretion by 11 or 30%, respectively in the finisher pig experiment. The proportion of water-soluble phosphorus in total phosphorus was not affected by dietary treatment in any of the three experiments. In conclusion, adding phytase at 1000 units/kg to a corn-soybean meal-based P-deficient diet basal diet containing no added inorganic P compared with B + P diet reduced the daily excretion of water-soluble P in starter, grower, and finisher pigs by 42, 34, and 30%, respectively. [source]

Nutrient utilization and manure P excretion in growing pigs fed corn-barley-soybean based diets supplemented with microbial phytase

ANIMAL SCIENCE JOURNAL, Issue 1 2009
Adewale EMIOLA
ABSTRACT The effect of high levels of microbial phytase supplementation in diets for growing pigs was studied in a 2-week performance and nutrient digestibility trial involving 28 growing pigs weighing 16.4 ± 1.06 (mean ± SD) kg. Seven corn-barley-soybean meal-based diets consisting of a positive control (PC) formulated to meet or exceed NRC nutrient requirements; a negative control (NC) with non-phytate P reduced by 0.1% unit from NRC requirement and fed without or with 500 or 1000 U/kg; a doubled negative control (DNC) with no added inorganic P and fed without or with 2000 or 4000 U/kg. Chromic oxide was added as an indigestible marker and all diets were fed as mash. Pigs fed the PC diet had a higher P digestibility compared with those fed the NC (P < 0.02) and the DNC (P < 0.001) diets. Supplementing the NC diet with pyhtase tended to improve P digestibility (P < 0.10). However, addition of phytase to the DNC diet resulted in linear (P < 0.001) and quadratic (P < 0.03) increases in P digestibility with an overall improvement of 8% and 121% at 4000 phytase U/kg of diet, respectively, compared with the PC and DNC diets. Apparent total tract digestibility of N, OM and DM were higher (P < 0.05) in the PC diet compared with the DNC diet, but not the NC diet (P < 0.10). No effect of phytase addition to NC was observed on Ca, N, DM and OM digestibility. Phytase addition to the DNC diet resulted in a linear increase (P < 0.05) in N, DM and OM digestibility but not Ca. Increasing the levels of phytase supplementation in the NC and the DNC diets linearly decreased fecal P (P < 0.05) content by 45 and 42%, respectively. Adding phytase at 1000 or 4000 U/kg increased P retention (P < 0.05) by 14.3 or 15.6% units, respectively, compared with the PC diet. Urinary P excretion was higher in the group fed the PC diet compared with those fed the NC and DNC diets (P < 0.05). The results of this study show that complete removal of inorganic P from growing pig diets coupled with phytase supplementation improves digestibility and retention of P and N, thus reducing manure P excretion without any negative effect on pig performance. [source]