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Microbial Life (microbial + life)
Selected AbstractsThe Dry Limit of Microbial Life in the Atacama Desert Revealed by Calorimetric ApproachesENGINEERING IN LIFE SCIENCES (ELECTRONIC), Issue 5 2008N. Barros Abstract The Atacama desert in Chile is one of the driest and most lifeless environments on Earth. It rains possibly once a decade. NASA examined these soils as a model for the Martian environment by comparing their degradation activity with Martian soil and looking for "the dry limit of life". The existence of heterotrophic bacteria in Atacama soil was demonstrated by DNA extraction and by the isolation of microorganisms. So far, however, no data have been available about the metabolic activities in these soils due to the limitations of the existing methodologies when applied to desert soils. Calorimetry was used to obtain information on the metabolic and thermal properties of eleven soil samples collected at different sites in the Atacama desert. Differential scanning calorimetry and isothermal calorimetry were employed to determine the pyrolysis properties of the carbon-containing matter and to measure biomass and microbial metabolism. They were compared to other soil properties such as total carbon and nitrogen, carbon to nitrogen ratio and pH. There was measurable organic matter in nine of the eleven samples and the heat of pyrolysis of those soils was correlated to the carbon content. In five of the eleven samples no biomass could be detected and the existence of basal microbial metabolism could not be established because all samples showed endothermic activity, probably from inorganic reactions with water. Six samples showed microbial activation after the addition of glucose. Carbon content, nitrogen content and the microbial activity after glucose amendment were correlated to the altitude and to the average minimum temperature of the sampling sites calculated from meteorological data. The detectable microbial metabolism was more dissipative with increasing altitude and decreasing temperature. [source] Microbial life in glacial ice and implications for a cold origin of lifeFEMS MICROBIOLOGY ECOLOGY, Issue 2 2007P. Buford Price Abstract Application of physical and chemical concepts, complemented by studies of prokaryotes in ice cores and permafrost, has led to the present understanding of how microorganisms can metabolize at subfreezing temperatures on Earth and possibly on Mars and other cold planetary bodies. The habitats for life at subfreezing temperatures benefit from two unusual properties of ice. First, almost all ionic impurities are insoluble in the crystal structure of ice, which leads to a network of micron-diameter veins in which microorganisms may utilize ions for metabolism. Second, ice in contact with mineral surfaces develops a nanometre-thick film of unfrozen water that provides a second habitat that may allow microorganisms to extract energy from redox reactions with ions in the water film or ions in the mineral structure. On the early Earth and on icy planets, prebiotic molecules in veins in ice may have polymerized to RNA and polypeptides by virtue of the low water activity and high rate of encounter with each other in nearly one-dimensional trajectories in the veins. Prebiotic molecules may also have utilized grain surfaces to increase the rate of encounter and to exploit other physicochemical features of the surfaces. [source] Microbial life in cold ecosystemsFEMS MICROBIOLOGY ECOLOGY, Issue 1 2005Max Häggblom No abstract is available for this article. [source] Amino acid sources in the adult diet do not affect life span and fecundity in the fruit-feeding butterfly Bicyclus anynanaECOLOGICAL ENTOMOLOGY, Issue 4 2008FREERK MOLLEMAN Abstract 1.,In tropical forests, the adults of many butterfly species feed on fruits rather than nectar from flowers and have long life spans. Rotting fruit and nectar differ from each other in many respects, including sources of amino acids and microbial life. If amino acids in the adult diet can be used for reproduction, this may have facilitated the evolution of extended life spans in this guild. 2.,This issue was addressed by investigating effects of banana, yeast, and amino acids in the adult diet of the fruit-feeding butterfly Bicyclus anynana (Lepidoptera) on longevity and female reproductive output in two experiments. 3.,Results showed that in the fruit-feeding butterfly B. anynana: (i) banana juice, but not sliced banana or added amino acids extend life span compared with a sugar solution of similar composition; (ii) compared with this sugar solution, other cohorts (banana juice-amino acid enriched) did not have significantly higher reproductive outputs; (iii) yeast does not represent a valuable source of nutrients; (iv) caloric restriction may cause decreased life span and rate of reproduction; and (v) increased rates of reproduction have a life span cost. [source] Biological energy requirements as quantitative boundary conditions for life in the subsurfaceGEOBIOLOGY, Issue 4 2004T. M. HOEHLER ABSTRACT All life requires energy, which must be extracted from the environment. For all known life, free energy must be available at finite minimum levels in order to be usefully harnessed and must be delivered at finite minimum rates in order to support basic biochemical integrity and function. While seldom tested in the high energy light- and oxygen-based metabolisms of the surface biosphere, the magnitude of these requirements , the biological energy quantum (BEQ) and maintenance energy (ME) requirements, respectively , is considerable with respect to the potential metabolisms and energy sources that characterize the deep subsurface realm. As such, they constitute a fundamental constraint on the possible nature, distribution, and activity of microbial life in that environment. Because the energy released in a chemical transformation can be equated to the concentrations of substrates and products, both the BEQ and ME requirements define the minimum substrate concentration and minimum substrate production rate that must be sustained by a given environment for it to be capable of supporting life. The magnitudes of the BEQ and ME requirements are sensitive to a range of environmental parameters that may vary significantly in the subsurface. Temperature exerts a particularly strong control and is among the most important parameters to be considered in evaluating the energetic habitability of subsurface environments. [source] Tectonic control of bioalteration in modern and ancient oceanic crust as evidenced by carbon isotopesISLAND ARC, Issue 1 2006Harald Furnes Abstract We review the carbon-isotope data for finely disseminated carbonates from bioaltered, glassy pillow rims of basaltic lava flows from in situ slow- and intermediate-spreading oceanic crust of the central Atlantic Ocean (CAO) and the Costa Rica Rift (CRR). The ,13C values of the bioaltered glassy samples from the CAO show a large range, between ,17 and +3, (Vienna Peedee belemnite standard), whereas those from the CRR define a much narrower range, between ,17, and ,7,. This variation can be interpreted as the product of different microbial metabolisms during microbial alteration of the glass. In the present study, the generally low ,13C values (less than ,7,) are attributed to carbonate precipitated from microbially produced CO2 during oxidation of organic matter. Positive ,13C values >0, likely result from lithotrophic utilization of CO2 by methanogenic Archaea that produce CH4 from H2 and CO2. High production of H2 at the slow-spreading CAO crust may be a consequence of fault-bounded, high-level serpentinized peridotites near or on the sea floor, in contrast to the CRR crust, which exhibits a layer-cake pseudostratigraphy with much less faulting and supposedly less H2 production. A comparison of the ,13C data from glassy pillow margins in two ophiolites interpreted to have formed at different spreading rates supports this interpretation. The Jurassic Mirdita ophiolite complex in Albania shows a structural architecture similar to that of the slow-spreading CAO crust, with a similar range in ,13C values of biogenic carbonates. The Late Ordvician Solund,Stavfjord ophiolite complex in western Norway exhibits structural and geochemical evidence for evolution at an intermediate-spreading mid-ocean ridge and displays ,13C signatures in biogenic carbonates similar to those of the CRR. Based on the results of this comparative study, it is tentatively concluded that the spreading rate-dependent tectonic evolution of oceanic lithosphere has a significant control on the evolution of microbial life and hence on the ,13C biosignatures preserved in disseminated biogenic carbonates in glassy, bioaltered lavas. [source] Archaeabacterial lipids in drill core samples from the Bosumtwi impact structure, GhanaMETEORITICS & PLANETARY SCIENCE, Issue 11 2008Marina ESCALA The Bosumtwi crater in Ghana (West Africa) is a relatively young (1.07 Myr) structure with a rim-to-rim diameter of about 10.5 km. In a preliminary study targeting the subsurface microbial life in the impact structure, seven samples of the impact breccia from the central uplift of the Bosumtwi crater were analyzed for the presence of typical archaeal membrane-lipids (GDGTs). These have been detected in four of the samples, at a maximum depth of 382 m below the lake surface, which is equivalent to 309 m below the surface sediment. The concentration of the GDGTs does not show a trend with depth, and their distribution is dominated by GDGT-0. Possible origins of these lipids could be related to the soils or rocks predating the impact event, the hydrothermal system generated after the impact, or due to more recent underground water [source] Geological overview and cratering model for the Haughton impact structure, Devon Island, Canadian High ArcticMETEORITICS & PLANETARY SCIENCE, Issue 12 2005Gordon R. Osinski Regional geological mapping has refined the sedimentary target stratigraphy and constrained the thickness of the sedimentary sequence at the time of impact to ,1880 m. New 40Ar,39Ar dates place the impact event at ,39 Ma, in the late Eocene. Haughton has an apparent crater diameter of ,23 km, with an estimated rim (final crater) diameter of ,16 km. The structure lacks a central topographic peak or peak ring, which is unusual for craters of this size. Geological mapping and sampling reveals that a series of different impactites are present at Haughton. The volumetrically dominant crater-fill impact melt breccias contain a calcite-anhydrite-silicate glass groundmass, all of which have been shown to represent impact-generated melt phases. These impactites are, therefore, stratigraphically and genetically equivalent to coherent impact melt rocks present in craters developed in crystalline targets. The crater-fill impactites provided a heat source that drove a post-impact hydrothermal system. During this time, Haughton would have represented a transient, warm, wet microbial oasis. A subsequent episode of erosion, during which time substantial amounts of impactites were removed, was followed by the deposition of intra-crater lacustrine sediments of the Haughton Formation during the Miocene. Present-day intra-crater lakes and ponds preserve a detailed paleoenvironmental record dating back to the last glaciation in the High Arctic. Modern modification of the landscape is dominated by seasonal regional glacial and niveal melting, and local periglacial processes. The impact processing of target materials improved the opportunities for colonization and has provided several present-day habitats suitable for microbial life that otherwise do not exist in the surrounding terrain. [source] Bacitracin sensing in Bacillus subtilisMOLECULAR MICROBIOLOGY, Issue 3 2008Eva Rietkötter Summary The extracellular presence of antibiotics is a common threat in microbial life. Their sensitive detection and subsequent induction of appropriate resistance mechanisms is therefore a prerequisite for survival. The bacitracin stress response network of Bacillus subtilis consists of four signal-transducing systems, the two-component systems (TCS) BceRS, YvcPQ and LiaRS, and the extracytoplasmic function (ECF) , factor ,M. Here, we investigated the mechanism of bacitracin perception and the response hierarchy within this network. The BceRS,BceAB TCS/ABC transporter module is the most sensitive and efficient bacitracin resistance determinant. The ABC transporter BceAB not only acts as a bacitracin detoxification pump, but is also crucial for bacitracin sensing, indicative of a novel mechanism of stimulus perception, conserved in Firmicutes bacteria. The Bce system seems to respond to bacitracin directly (drug sensing), whereas the LiaRS TCS and ,M respond only at higher concentrations and indirectly to bacitracin action (damage sensing). The YvcPQ,YvcRS system is subject to cross-activation via the paralogous Bce system, and is therefore only indirectly induced by bacitracin. The bacitracin stress response network is optimized to respond to antibiotic gradients in a way that maximizes the gain and minimizes the costs of this stress response. [source] How microbes utilize host ubiquitinationCELLULAR MICROBIOLOGY, Issue 10 2009Thomas Spallek Summary Activity, abundance and localization of eukaryotic proteins can be regulated through covalent attachment of ubiquitin and ubiquitin-like moieties. Ubiquitination is important in various aspects of immunity. Pathogens utilize host ubiquitination for the suppression of immune signalling and reprogramming host processes to promote microbial life. They deliver so-called effector molecules into host cells, which functionally or structurally resemble components of the host ubiquitination machinery utilizing this enzymatic process or they secrete molecules to inhibit ubiquitin-mediated degradation. Since prokaryotic pathogens lack a classical ubiquitination system, effector mimicry of components of the ubiquitin machinery could be achieved through gene flow. Horizontal gene transfer allows pathogenic bacteria to access ubiquitination enzymes from a potential host, while lateral gene transfer recruits components from another pathogen providing spread within the microbial community. Additionally, convergent evolution can shape bacterial proteins to acquire ubiquitination functions. [source] | |||||||||||||