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Microbial Association (microbial + association)

Distribution by Scientific Domains

Life Sciences	75%

Selected Abstracts

Bioremediation of 6 % [w/w] Diesel-Contaminated Mainland Soil in Singapore: Comparison of Different Biostimulation and Bioaugmentation Treatments

ENGINEERING IN LIFE SCIENCES (ELECTRONIC), Issue 1 2006
M. Mathew
Abstract The efficacy of indigenous microorganisms to degrade diesel oil in contaminated mainland sites in Singapore was investigated. A semi-scale trial was made by spiking topsoil with 6,% [w/w] of diesel oil. The results indicated that in the presence of NPK commercial (Rosasol®) fertilizer a 53,% reduction in contaminant concentration was recorded after 60,days compared to untreated controls while the addition of a mixture of urea and K2HPO4 effected a 48,% reduction in the Total Recoverable Petroleum Hydrocarbons. A commercial culture and an enriched/isolated microbial association proved to be the least effective with 25 and 9,% reductions, respectively. The results confirmed the bioremediation potential of indigenous microorganisms for diesel-oil contaminated mainland soil. Identification of the persistent compounds was done and perceived as a tool in decision-making on strategies for speeding up of the degradation process to achieve clean-up standards in shorter remediation periods. [source]

Microbial characterisation and stability of a farmhouse natural fermented milk from Spain

INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, Issue 3 2010
ÁNGEL ALEGRÍA
This work reports the microbial characterisation of a farmhouse natural fermented milk (NFM) with good sensorial properties produced in Spain. Culturing and denaturing gradient gel electrophoresis (DGGE) analyses showed thatLactococcus lactissubsp.lactis and L. lactissubsp.cremoris(approximate levels of 109 cfu/mL) were dominant in this NFM, whileLactobacillus plantarumappeared at a lower level (106,107 cfu/mL). Repetitive extragenic palindromic (REP)-PCR typing of the isolates identified single strains each ofLc. lactissubsp.lactis, Lc. lactissubsp.cremorisandLb. plantarum. These three strains formed a stable microbial association which has been maintained for at least some decades. [source]

Ex situ cultivation of Aplysinaaerophoba close to in situ conditions: ecological, biochemical and histological aspects

MARINE ECOLOGY, Issue 2 2008
Anne Klöppel
Abstract Sponges provide the largest number of biologically active natural products known from the marine environment and continue to be a very well studied phylum of marine fauna. The Mediterranean sponge Aplysina aerophoba accumulates brominated isoxazoline alkaloids such as Aplysinamisin-1, Aerophobin-2, Isofistularin-3 and the biotransformation product Aeroplysinin-1, which possesses, for example, antibiotic and cytotoxic properties. Until now, it is still being discussed which organisms , the sponge itself or associated microorganisms , are responsible for metabolite production. For cultivating Aplysina individuals under ex situ conditions, we surveyed relevant ecological factors in situ and controlled them in our aquarium system. We maintained A. aerophoba for more than 9 months and analysed changes of metabolite content and composition, microbial association as well as morphology in situ and ex situ under different light exposure. Although sponges showed slight reduction during maintenance, ex situ cultivation similar to in situ conditions provides a promising method to keep sponges and obtain their bioactive metabolites. [source]

Role of bacteria in the oviposition behaviour and larval development of stable flies

MEDICAL AND VETERINARY ENTOMOLOGY, Issue 1 2006
A. Romero
Abstract., Stable flies, Stomoxys calcitrans (L.), are the most important pests of cattle in the United States. However, adequate management strategies for stable flies, especially for pastured cattle, are lacking. Microbial/symbiont-based approaches offer novel venues for management of insect pests and/or vector-borne human and animal pathogens. Unfortunately, the fundamental knowledge of stable fly,microbial associations and their effect on stable fly biology is lacking. In this study, stable flies laid greater numbers of eggs on a substrate with an active microbial community (> 95% of total eggs oviposited) than on a sterilized substrate. In addition, stable fly larvae could not develop in a sterilized natural or artificial substrate/medium. Bacteria were isolated and identified from a natural stable fly oviposition/developmental habitat and their individual effect on stable fly oviposition response and larval development was evaluated in laboratory bioassays. Of nine bacterial strains evaluated in the oviposition bioassays, Citrobacter freundii stimulated oviposition to the greatest extent. C. freundii also sustained stable fly development, but to a lesser degree than Serratia fanticola. Serratia marcescens and Aeromonas spp. neither stimulated oviposition nor supported stable fly development. These results demonstrate a stable fly bacterial symbiosis; stable fly larval development depends on a live microbial community in the natural habitat, and stable fly females are capable of selecting an oviposition site based on the microbially derived stimuli that indicate the suitability of the substrate for larval development. This study shows a promising starting point for exploiting stable fly,bacterial associations for development of novel approaches for stable fly management. [source]