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Mean Corpuscular Haemoglobin Concentration (mean + corpuscular_haemoglobin_concentration)
Selected AbstractsThe effect of weekly iron supplementation on anaemia and on iron deficiency among female tea pluckers in BangladeshJOURNAL OF HUMAN NUTRITION & DIETETICS, Issue 3 2001D. Gilgen Aim To investigate the effect of weekly iron supplementation on anaemia and iron deficiency among adult, female tea pluckers. Method A randomized double-blind intervention trial was conducted in a tea estate in Bangladesh where a total of 280 women received either weekly iron supplementation (200 mg ferrous fumarate and 200 mg folic acid) for 24 weeks or a matching placebo. Capillary blood samples were drawn at baseline and post-trial to determine haemoglobin, haematocrit and ferritin concentration. Mean corpuscular haemoglobin concentration (MCHC) was calculated using the haemoglobin and haematocrit values. Results The mean haemoglobin concentration in the supplemented group increased by 5.52 g L,1 over the study period, on average, while ferritin values decreased by 0.33 ,g L,1. The control group showed a decrease in both mean haemoglobin (,0.24 g L,1) and ferritin (,5.32 ,g L,1). Those individuals in the supplemented group with the lowest pretrial haemoglobin and ferritin values experienced the greatest improvements post-trial, whereas nonanaemic individuals showed a decrease in both haemoglobin and ferritin concentrations. A total of 62.2% of women in the supplemented group reported feeling better and more energetic compared to 51.1% in the placebo group; 14.4% of the supplemented group and 22.7% of the control group complained about side-effects. Conclusion Weekly iron supplementation was logistically simpler and cheaper than daily supplementation but would have to be continued on a longer term basis in order to combat both anaemia and iron deficiency. [source] Growth and haematology of pacu, Piaractus mesopotamicus, fed diets with varying protein to energy ratioAQUACULTURE RESEARCH, Issue 4 2009Álvaro José De Almeida Bicudo Abstract Haematopoiesis and blood cells' functions can be influenced by dietary concentration of nutrients. This paper studied the effects of dietary protein:energy ratio on the growth and haematology of pacu, Piaractus mesopotamicus. Fingerling pacu (15.5±0.4 g) were fed twice a day for 10 weeks until apparent saciety with diets containing 220, 260, 300, 340 or 380 g kg,1 crude protein (CP) and 10.88, 11.72, 12.55, 13.39, 14.22 MJ kg,1 digestible energy (DE) in a totally randomized experimental design, 5 × 5 factorial scheme (n=3). Weight gain and specific growth rate were affected (P<0.05) by protein level only. Protein efficiency ratio decreased (P<0.05) with increasing dietary protein at all levels of dietary energy. Daily feed intake decreased (P<0.05) with increasing dietary energy. Mean corpuscular haemoglobin concentration was affected (P<0.05) by DE and interaction between dietary CP and DE. Total plasma protein increased (P<0.05) with dietary protein and energy levels. Plasma glucose decreased (P<0.05) with increasing dietary protein. The CP requirement and optimum protein:energy ratio for weight gain of pacu fingerlings, determined using broken-line model, were 271 g kg,1 and 22.18 g CP MJ,1 DE respectively. All dietary CP and DE levels studied did not pose damages to fish health. [source] Haematological reference values in Spanish adolescents: the AVENA studyEUROPEAN JOURNAL OF HAEMATOLOGY, Issue 6 2009Javier Romeo Abstract Objectives:, To provide reference values for haematological indices in Spanish adolescents according to age and gender. Methods:, A cross sectional study conducted in five Spanish cities was performed. Blood was drawn from a representative sample of 581 adolescents with age ranging from 13 to 17,18.5 yr. Age- and gender-specific means, standard deviations and percentiles were determined for the following parameters: total red blood cell counts (RBC), haemoglobin concentration (Hb), haematocrit percentage (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, red cell distribution width and total white blood cell (WBC) counts as well as counts and percentage of neutrophils, lymphocytes, monocytes, eosinophils and basophils; platelet count (PLT), mean platelet volume and plateletcrit percentage. Results:, Younger male subjects presented lower RBC, Hb, Hct and MCV means that their older counterpart. By contrast these differences were not observed in female subjects. As expected, RBC, Hb and Hct mean values in males were found significantly higher than in girls for all studied age groups. No significant differences were observed in WBC by age and gender. PLT values gradually decreased with age, except for females aged 17,18.5 yr. Conclusion:, The present study provides reference data on the distribution of haematological indices of Spanish adolescents. These data can be useful biomarkers of the nutritional status in adolescents. [source] Respiratory changes in human red cellsINTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, Issue 6 2001G. Aliberti To investigate physiological respiratory changes in human red cells, we measured automated red cell parameters in samples from the pulmonary and radial arteries of 86 patients undergoing aorto-coronary bypass and from the pulmonary artery and the aorta in 23 patients. Our results showed higher mean corpuscular volume (88.53 ± 5.06 fl vs. 88.12 ± 4.94 fl, P < 0.000001), haematocrit (0.369 ± 0.039 vs. 0.367 ± 0.038, P < 0.0005), red cell distribution width (43.38 ± 4.16 vs. 43.04 ± 4.05 fl, P < 0.000001) and a lower mean corpuscular haemoglobin concentration (338.3 ± 15.9 vs. 339.9 ± 16.8 g/l, P < 0.005) in pulmonary arterial as compared to radial arterial blood. There were no differences with respect to haemoglobin concentration, red blood cell count, or mean corpuscular haemoglobin. Similar differences were observed between pulmonary arterial and aortic blood. Our results suggest cyclic respiratory modifications of red cell parameters attributable to the CO2 Jacobs,Stewart cycle. [source] Derivated fetal haemoglobin as a marker for red cell age in the human fetus reflecting stimulated or impaired red blood cell productionPRENATAL DIAGNOSIS, Issue 7 2001Margriet Huisman Abstract We have determined whether derivated fetal haemoglobin (dHbF, consisting of glycated and acetylated HbF) can be used as a cell age marker for fetal red blood cells (RBCs). Cord blood was obtained between 19 and 39 weeks of gestation from 28 alloimmunised anaemic fetuses (23 RhD+ and 5,Kell) and from 20 non-anaemic fetuses and newborns (controls). Density gradient centrifugation was applied to 36 samples (20 RhD+, 15 controls and 1,Kell) to obtain fractions of increasing cell age. Blood samples were used for measurements of mean cellular volume (MCV), mean cell haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), pyruvate kinase activity (PK) and derivated fetal haemoglobin (dHbF) by cation-exchange HPLC. Reticulocytes were counted only in the whole blood samples. In all density gradient separated RBC fractions, the values for MCV, MCH and PK activity decreased and those of MCHC and dHbF increased with increasing density (equivalent to increasing cell age). The mean density was lower for RBCs of the anaemic RHD group (1.072±0.007,g/ml) than for the non-anaemic controls (1.077±0.005,g/ml) (p<0.05) The RBC density of the Kell sensitised fetus did not differ from those of the controls. In the control group, the values of the cell age markers in whole blood changed significantly with the gestational age, showing an increase of mean age of the erythrocyte population. The best linear relationship was found for dHbF (y=6.28+0.17*weeks; r=0.84; p<0.001). In the anaemic RhD+ fetuses, the RBC age markers did not change with gestational age; the dHbF percentages were lower, and the MCV, MCH, PK values and the reticulocyte counts were higher than in the controls (0.05 Tolerance of Atlantic salmon (Salmo salar) to dietborne endosulfan assessed by haematology, biochemistry, histology and growthAQUACULTURE NUTRITION, Issue 5 2010A.-K. LUNDEBYE Abstract The inclusion of plant-based ingredients in commercial fish feeds may pose a challenge because of the presence of undesirable substances, such as the pesticide endosulfan. Waterborne endosulfan is highly toxic to fish, whereas dietborne exposure has varied toxicity in different species. To investigate the systemic effects of endosulfan exposure, quadruplicate groups of Atlantic salmon (Salmo salar) were fed either 0 (control), 0.005 mg kg,1; the European Union's maximum limit, or 10 or 20 times this level (0.05 and 0.1 mg kg,1 respectively) for 95 days. There were no significant differences (P > 0.05) in liver somatic index, spleen somatic index, condition factor or growth among treatments. There were no indications of liver damage in fish from any of the groups in the biomarkers measured: plasma aspartate aminotransferase, plasma alanine aminotransferase and histopathology. Similarly, there were no apparent treatment-related effects on the haematological parameters Hct, Hb, mean corpuscular volume, mean corpuscular haemoglobin concentration and mean corpuscular haemoglobin, and blood sodium, potassium, calcium and chloride levels were not significantly (P > 0.05) different among groups. Lipid digestibility, but not energy, protein, or glycogen digestibility, was significantly (P < 0.05) reduced at the highest exposure concentration. However, no significant differences were observed in lipid production value or lipid efficiency ratio. In contrast to previous studies, clinical histological abnormalities were not observed in the intestine, liver or spleen of endosulfan-treated fish. [source] Growth, haematological parameters and tissue lipid peroxidation of soft-shelled turtles, Pelodiscus sinensis, fed diets supplemented with different levels of ferrous sulphateAQUACULTURE NUTRITION, Issue 1 2009J.-H. CHU Abstract Soft-shelled turtles, Pelodiscus sinensis, with an average weight of 5.55 g, were fed diets supplemented with eight levels of ferrous sulphate for 8 weeks. The analysed iron content ranged from 50.8 to 482.9 mg kg,1. Growth rate of turtles fed the control diet with no iron supplementation was the lowest among all dietary groups. Haematological parameters including red blood cell, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration of the turtles fed the control diet were also significantly (P < 0.05) lower relative to the other groups. Thus, dietary iron at 50.8 mg kg,1 (no supplemented iron) was deemed deficient for growth and ineffective at preventing anaemia in juvenile soft-shelled turtle. Whereas, a supplementation of 50 mg kg,1 ferrous sulphate (a total dietary iron of 91.8 mg kg,1) was enough to normalize the haematological values of soft-shelled turtles to the level similar to other iron supplement-fed groups. Within the tested dietary iron range, liver iron content curve-linearly (r2 = 0.99) increased with increasing dietary iron level. Furthermore, thiobarbituric acid-reactive substances in liver tissues of the turtles have also increased when liver iron content increased. The dietary iron requirement of soft-shelled turtle is 120,198 mg kg,1 when ferrous sulphate is used as the source of iron. [source] Haematologic and immunologic parameters of bullfrogs, Lithobates catesbeianus, fed probioticsAQUACULTURE RESEARCH, Issue 7 2010Danielle de Carla Dias Abstract The effects of two probiotics (P1,Lactobacillus acidophilus, Bifidobacterium bifidum and Enterococcus faecium and P2,Bacillus subtilis) supplemented to commercial feed (40% crude protein) on the haematological and immunological parameters of the bullfrog Lithobates catesbeianus were studied. Two doses of each probiotic (5 and 10 g kg,1 of food) were added to the diets and fed to frogs, totalling five treatments over 112 days. Haematological analyses consisted of total and differential leucocyte counts, erythrocyte and thrombocyte counts, haematocrit, haemoglobin levels and RBC indices (mean corpuscular volume, mean corpuscular haemoglobin , and mean corpuscular haemoglobin concentration) and the immunological parameters included phagocytic capacity and phagocytic index of peritoneal phagocytes. The results showed that the probiotics did not significantly influence any of the haematological parameters measured. However, immunological assays showed that the probiotics had an immunostimulating effect. The greatest effects were seen with probiotic P1 fed at a dose of 10 g kg,1 of diet and probiotic P2 fed at 5 g kg,1 of diet. [source] Growth efficiency, body composition, survival and haematological changes in great sturgeon (Huso huso Linnaeus, 1758) juveniles fed diets supplemented with different levels of ErgosanAQUACULTURE RESEARCH, Issue 7 2009Mohammad Ali Jalali Abstract Growth performance, carcass quality, survival and haematological responses were determined when Huso huso juvenile (41.7±1.8 g) fed diets containing Ergosan (an algal product) at 0, 2.0, 4.0 and 6.0 g kg,1 for 60 days. Each diet was fed to triplicate groups of fish at 10-day intervals (1,10, 20,30 and 40,50 with non-supplemented diets and 10,20, 30,40 and 50,60 with supplemented diets). Results showed that fish fed diets containing Ergosan had significantly higher growth than the control group (P<0.05). Survival was not different among all dietary treatments (P>0.05). Food conversion ratio in the fish fed a diet containing 4.0 and 6.0 g kg,1 Ergosan was significantly better than the other treatments (P<0.05), whereas protein efficiency ratio was not different between experimental diets. Lymphocyte count in the fish fed diets containing Ergosan was higher than the other treatments. Haematocrit, haemoglobin (Hb) concentration, number of erythrocytes, total leucocytes, monocyte, eosinophil, myelocyte, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were not different between treatments. Neutrophil count in the control group was higher than the fish fed supplemented diets. Furthermore, whole body lipid, moisture and fibre were not different among dietary treatments (P>0.05) but body protein in the fish fed a diet containing Ergosan at the level of 2.0 and 4.0 g kg,1 was higher than the other treatments. Whole body ash content was higher in the control group. It was concluded that dietary administration of Ergosan can influence some growth and haematological parameters in great sturgeon, H. huso juveniles. [source] Effects of sulfamerazine on selected haematological and immunological parameters in rainbow trout (Onchorhynchus mykiss, Walbaum, 1792)AQUACULTURE RESEARCH, Issue 4 2009Naim Saglam Abstract In the present study, effects of sulfamerazine on some haematological and immunological parameters of rainbow trout (Oncorhynchus mykiss) were examined. Four groups of rainbow trout were fed experimental diets containing either no sulfamerazine (control) or supplemented with sulfamerazine at 100 mg kg,1 (Exp-A), 200 mg kg,1 (Exp-B) or 400 mg kg,1 (Exp-C) for 21 days. Blood samples were taken for the haematological and immunological parameters from fish on the third, seventh, 14th and 21st days of feeding. Haematological parameters [haematocrit, leucocrit, numbers of erythrocytes (RBC) and leucocytes (WBC), haemoglobin (Hb), mean corpuscular volume (MCV), mean haemoglobin concentration (MHC) and mean corpuscular haemoglobin concentration (MCHC)] and immunological parameters [phagocytic ratio (PR) and index (PI), glass-adherent NBT-positive cell activation, total plasma protein and total immunoglobulin (Ig)] were evaluated during the experimental trial. It has been observed that MCV (P<0.05), PR and PI (P>0.05) were increased and haematocrit and leucocrit value, numbers of erythrocytes and leucocyte, haemoglobin, MHC and MCHC values and total plasma protein and total Ig levels were decreased in rainbow trout after application of three different doses of sulfamerazine. [source]
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