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Mechanistic Work (mechanistic + work)

Distribution by Scientific Domains
Chemistry88%

Selected Abstracts

Resource allocation-based life histories: A conceptual basis for studies of ecological toxicology,

ENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 8 2001
Justin D. Congdon
Abstract Whereas ecological assessments of contaminants are concerned with populations and higher levels of organization, most mechanistic work in toxicology is directed at effects on individuals and their parts. We propose that studies based on individuals can be useful in ecological analysis of polluted systems when based on the concepts of resource allocation-based life history analysis. At the heart of the resource allocation approach is the concept of operative environments of individuals (i.e., environmental factors influencing birth, death, or migration). Contaminants can have strong influences on operative environments, modifying resource allocation strategies that reflect changes in energy assimilation and demands. By examining contaminant-induced responses of individuals from the perspective of changing operative environments, individual-based changes and population dynamics can be addressed in an ecologically rigorous manner. [source]

Cover Picture: (Adv. Synth.

ADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 16 2007
Catal.
The cover picture results from the seminal mechanistic work on DERA, deoxyribose-5-phosphate aldolase, a widely distributed catabolic enzyme, by Chi-Huey Wong and co-workers. [source]

Cover Picture: (Adv. Synth.

ADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 13 2007
Catal.
The cover picture results from the seminal mechanistic work on DERA, deoxyribose-5-phosphate aldolase, a widely distributed catabolic enzyme, by Chi-Huey Wong and co-workers. [source]

Cover Picture: (Adv. Synth.

ADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 8-9 2007
Catal.
The cover picture results from the seminal mechanistic work on DERA, deoxyribose-5-phosphate aldolase, a widely distributed catabolic enzyme, by Chi-Huey Wong and co-workers. [source]

Cover Picture: (Adv. Synth.

ADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 7 2007
Catal.
The cover picture results from the seminal mechanistic work on DERA, deoxyribose-5-phosphate aldolase, a widely distributed catabolic enzyme, by Chi-Huey Wong and co-workers. [source]

Cover Picture: (Adv. Synth.

ADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 6 2007
Catal.
The cover picture results from the seminal mechanistic work on DERA, deoxyribose-5-phosphate aldolase, a widely distributed catabolic enzyme, by Chi-Huey Wong and co-workers. [source]

Cover Picture: (Adv. Synth.

ADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 3 2007
Catal.
The cover picture results from the seminal mechanistic work on DERA, deoxyribose-5-phosphate aldolase, a widely distributed catabolic enzyme, by Chin-Huey Wong and co-workers. [source]

Cover Picture: (Adv. Synth.

ADVANCED SYNTHESIS & CATALYSIS (PREVIOUSLY: JOURNAL FUER PRAKTISCHE CHEMIE), Issue 1-2 2007
Catal.
The cover picture results from the seminal mechanistic work on DERA, deoxyribose-5-phosphate aldolase, a widely distributed catabolic enzyme, by Chin-Huey Wong and co-workers. [source]

Shear-induced degradation of plasmid DNA

JOURNAL OF PHARMACEUTICAL SCIENCES, Issue 7 2002
C. S. Lengsfeld
Abstract The majority of gene therapy clinical trials use plasmid DNA that is susceptible to shear-induced degradation. Many processing steps in the extraction, purification, and preparation of plasmid-based therapeutics can impart significant shear stress that can fracture the phosphodiester backbone of polynucleotides, and reduce biological activity. Much of the mechanistic work on shear degradation of DNA was conducted over 30 years ago, and we rely heavily on this early work in an attempt to explain the empirical observations of more recent investigations concerning the aerosolization of plasmids. Unfortunately, the sporadic reports of shear degradation in the literature use different experimental systems, making it difficult to quantitatively compare results and reach definitive mechanistic conclusions. In this review, we describe the forces imparted to DNA during shear stress, and use published data to quantitatively evaluate their relative effects. In addition, we discuss the effects of molecular weight, strain rate, particle size, flexibility, ionic strength, gas,liquid interfaces, and turbulence on the fluid flow degradation of supercoiled plasmid DNA. Finally, we speculate on computational methods that might allow degradation rates in different experimental systems to be predicted. © 2002 Wiley-Liss Inc. and the American Pharmaceutical Association J Pharm Sci 91:1581,1589, 2002 [source]