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Mechanical Inoculation (mechanical + inoculation)
Selected AbstractsIdentification and Characterization of a Potyvirus Isolated from Siratro PlantsJOURNAL OF PHYTOPATHOLOGY, Issue 4 2008L. J. Regatieri Abstract The present work describes the identification and characterization of a potyvirus isolated from siratro (Macroptilium atropurpureum Urb.) in the north-west region of the State of São Paulo, Brazil. The virus was transmitted by mechanical inoculation. Its host range was restricted mainly to members of the Fabaceae. A cDNA fragment of about 930 bp was amplified by RT/PCR, cloned and sequenced. The fragment, which included the coat protein gene, had amino acid identity percentages between 88 and 98% with isolates of Bean common mosaic virus (BCMV). Phylogenetic analysis grouped the siratro potyvirus and BCMV isolates in 99% of the replicates, including Azuki mosaic virus, Dendrobium mosaic virus, Blackeye cowpea mosaic virus and Peanut stripe virus, which have been classified as BCMV strains. This is the first citation on the presence of BCMV in siratro plants in Brazil. [source] Turnip yellow mosaic virus: transfer RNA mimicry, chloroplasts and a C-rich genomeMOLECULAR PLANT PATHOLOGY, Issue 5 2004THEO W. DREHER SUMMARY Taxonomy:,Turnip yellow mosaic virus (TYMV) is the type species of the genus Tymovirus, family Tymoviridae. TYMV is a positive strand RNA virus of the alphavirus-like supergroup. Physical properties:, Virions are non-enveloped 28-nm T = 3 icosahedrons composed of a single 20-kDa coat protein that is clustered in 20 hexameric and 12 pentameric subunits. Infectious particles and empty capsids coexist in infected tissue. The genomic RNA is 6.3 kb long, with a 5,m7GpppG cap and a 3, untranslated region ending in a tRNA-like structure to which valine can be covalently added. The genome has a distinctive skewed C-rich, G-poor composition (39% C, 17% G). Viral proteins:, Two proteins, whose open reading frames extensively overlap, are translated from the genomic RNA. p206, which contains sequences indicative of RNA capping, NTPase/helicase and polymerase activities, is the only viral protein that is necessary for genome replication in single cells. It is produced as a polyprotein and self-cleaved to yield 141- and 66-kDa proteins. p69 is required for virus movement within the plant and is also a suppressor of gene silencing. The coat protein is expressed from the single subgenomic RNA. Hosts and symptoms:, TYMV has a narrow host range almost completely restricted to the Cruciferae. Experimental host species are Brassica pekinensis (Chinese cabbage) or B. rapa (turnip), in which diffuse chlorotic local lesions and systemic yellow mosaic symptoms appear. Arabidopsis thaliana can also be used. Clumping of chloroplasts and the accumulation of vesicular invaginations of the chloroplast outer membranes are distinctive cytopathological symptoms. High yields of virus are produced in all leaf tissues, and the virus is readily transmissible by mechanical inoculation. Localized transmission by flea beetles may occur in the field. [source] Epidemiological evidence that vegetatively propagated, solanaceous plant species act as sources of Potato spindle tuber viroid inoculum for tomatoPLANT PATHOLOGY, Issue 1 2010J. Th. In autumn 2006 in the Netherlands, Potato spindle tuber viroid (PSTVd) infections were detected in 42·3 and 71·9% of professionally grown lots of Brugmansia spp. and Solanum jasminoides respectively. The infected lots contained 73 985 and 431 374 plants, respectively, demonstrating the presence of many potential viroid sources for tomato (Solanum lycopersicum). PSTVd was identified in cultivars of Brugmansia × candida, B. × flava, B. sanguinea, B. suaveolens and unspecified Brugmansia species/cultivars. Most infected lots of Brugmansia spp. originated from a single Dutch nursery; most infected lots of S. jasminoides originated abroad. Sequence analysis revealed that the PSTVd genomes from Brugmansia spp. contained an average of 360 nt, whereas all genomes from S. jasminoides except one consisted of 357 nt. Furthermore, the collective PSTVd genotypes showed polymorphism at four or more positions, except for two cases in which genotypes from Brugmansia spp. and S. jasminoides were identical. Phylogenetic studies showed that PSTVd genotypes from Brugmansia spp. and S. jasminoides grouped apart from each other and from PSTVd isolates from potato (Solanum tuberosum) and Physalis peruviana. The PSTVd genotypes from tomato did not form a separate cluster, but were dispersed over clusters of vegetatively or partly vegetatively propagated plant species, i.e. potato, P. peruviana and S. jasminoides. Moreover, mechanical inoculation of the predominant PSTVd genotypes from S. jasminoides to tomato was successful. These results provide evidence that vegetatively propagated, solanaceous plant species have been sources of infection for tomato crops in the past. [source] Molecular characterization of the CP gene and 3,UTR of Chilli veinal mottle virus from South and Southeast AsiaPLANT PATHOLOGY, Issue 3 2008W. S. Tsai Twenty-four isolates of Chilli veinal mottle virus (ChiVMV) from China, India, Indonesia, Taiwan and Thailand were analysed to determine their genetic relatedness. Pathogenicity of virus isolates was confirmed by induction of systemic mosaic and/or necrotic ringspot symptoms on Capsicum annuum after mechanical inoculation. The 3, terminal sequences of the viral genomic RNA were determined. The coat protein (CP) coding regions ranged from 858 to 864 nucleotides and the 3, untranslated regions (3,UTR) from 275 to 289 nucleotides in length. All isolates had the inverted repeat sequence GUGGNNNCCAC in the 3,UTR. The DAG motif, conserved in aphid-transmitted potyviruses, was observed in all isolates. All 24 isolates were considered as belonging to ChiVMV because of their high CP amino acid and nucleotide identity (more than 94·8 and 89·5%, respectively) with the reported ChiVMV isolates including the pepper vein banding virus (PVBV), the chilli vein-banding mottle virus (CVbMV) and the CVbMV Chiengmai isolate (CVbMV-CM1). Based on phylogenetic analysis, ChiVMV isolates including all 24 isolates tested, PVBV, CVbMV and CVbMV-CM1 can be classified into three groups. In addition, a conserved region of 204 amino acids with more than 90·2% identity was identified in the C terminal of the CP gene of ChiVMV and Pepper veinal mottle virus (PVMV), and may explain the serological cross reaction between these two viruses. The conserved region may also provide useful information for developing transgenic resistance to both ChiVMV and PVMV. [source] Characterization of Potato rough dwarf virus and Potato virus P: distinct strains of the same viral species in the genus CarlavirusPLANT PATHOLOGY, Issue 6 2006C. Nisbet In an attempt to resolve whether two putative carlaviruses, potato rough dwarf virus (PRDV) and potato virus P (PVP), reported infecting potato in Argentina and Brazil, respectively, are the same virus in the genus Carlavirus, they were characterized using nucleotide sequence analysis, serology and bioassay. For PRDV and PVP, sequences of 2016 and 1492 bp, respectively, were obtained. Similarity searches of coat-protein amino acid sequences and the presence of the nucleic-acid-binding protein exhibiting the characteristic motif C-X2 -C-X11 -C-X4 -C, highly conserved in carlaviruses, showed PRDV and PVP to be members of the genus Carlavirus. Further analysis showed that they were the same virus species since the full coat-protein sequence, translated from the nucleotide sequence, exceeded the 84% minimum identity required for members of the same species within the genus Carlavirus, at 90·8% identity in an ungapped alignment of 304 amino acids. Moreover, the viruses could not be distinguished using polyclonal antibodies raised to PRDV and PVP in ELISA. They could, however, be distinguished using an anti-PVP monoclonal antibody, which did not react to PRDV, and by differences in the susceptibility of plant species and potato cultivars to infection and/or symptom expression following mechanical inoculation. Host plants Datura metel, Nicandra physalodes and Nicotiana edwardsonii were reliably infected systemically with PVP, but not with PRDV. Although all potato cultivars tested were infected, there were marked differences between cultivars in the symptoms produced. The cultivars Ditta and King Edward showed symptoms (including stunting and mottle) when infected with PRDV, but not when infected with PVP. Based on the results, it is proposed that PRDV is a strain of PVP within the genus Carlavirus. For detection of the viruses in postentry quarantine, it is recommended that PRDV or PVP polyclonal antibodies are used, together with inoculation to Nicotiana occidentalis N. megalosiphon -P1. For differentiation of PRDV and PVP, the PVP monoclonal antibody may be used together with inoculation to D. metel and N. physalodes (systemic infection with PVP but not PRDV, determined using ELISA). [source] Identification and transmission of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) in Sri LankaPLANT PATHOLOGY, Issue 5 2002D. P. P. De Silva Sri Lankan black pepper with symptoms of yellow mottle disease contained a mixture of viruses: Piper yellow mottle virus (PYMV) particles (30 × 130 nm), Cucumber mosaic virus (CMV, 30 nm diameter isometric particles), and unidentified, isometric virus-like particles (30 nm diameter). An effective purification procedure is described for PYMV. Immunosorbent and conventional electron microscopy successfully detected badnavirus particles only when at least partially purified extracts were used. PYMV was confirmed as the cause of the disease, with the other two viruses apparently playing no part in producing symptoms. PYMV was transmitted by grafting, by the insect vectors citrus mealy bug (Planococcus citri) and black pepper lace bug (Diconocoris distanti), but not by mechanical inoculation or through seeds. The CMV isolate was transmitted to indicator plants by mechanical inoculation and by the vector Aphis gossypii, but not by Myzus persicae; but neither mechanical nor insect transmission of CMV to black pepper was successful. A sensitive polymerase chain reaction assay was developed to detect PYMV in black pepper. [source] Systemic induced resistance in Monterey pineFOREST PATHOLOGY, Issue 2 2001Bonello The pathogenic fungus Fusarium circinatum causes pitch canker of pines. This study shows that Monterey pine (Pinus radiata), one of the most economically important pine species in the world and the main host in California, responds to infection by Fusarium circinatum in a manner consistent with systemic induced resistance. Repeated mechanical inoculations of the same trees in the field produced progressively smaller lesions over a period of 2 years, with mean lesion length decreasing significantly from 2.89 ± 0.42 cm to 1.04 ± 0.17 cm. In the greenhouse, predisposing inoculations with the pathogen induced a significant lesion length reduction, from 5.5 ± 0.21 cm in control trees to 4.46 ± 0.36 cm in predisposed trees over a period of 6 weeks. Under constant environmental conditions in a growth chamber, predisposing inoculations also induced a significant reduction in lesion size, from 3.01 ± 0.15 cm to 2.55 ± 0.18 cm over a period of 4 weeks. This is the first unequivocal report of systemic induced resistance in a conifer. Résistance systémique induite chez le Pinus radiata Fusarium circinatum est l'agent causal du ,pitch canker' des pins. Le Pinus radiata est l'un des pins les plus importants économiquement dans le monde, et le principal hôte de la maladie en Californie. Cette étude montre que P. radiata réagit régulièrement à l'infection de Fusarium circinatum d'une façon qui peut être de la résistance systémique induite. Des inoculations mécaniques répétées sur de mêmes arbres en nature ont produit progressivement des lésions dont la taille diminuait au cours d'une période de deux ans; la longueur des lésions diminuait significativement de 2.89 ± 0.42 cm à 1.04 ± 0.17 cm. En serre, des inoculations de pré-conditionnement avec le parasite ont entraîné une réduction significative de la longueur des lésions, de 5.5 ± 0.21 cm chez les témoins à 4.46 ± 0.36 cm chez les arbres pré-conditionnés, au cours d'une période de six semaines. En conditions environnementales constantes en chambre climatique, les inoculations de pré-conditionnement ont aussi induit une réduction significative de la taille des lésions, de 3.01 ± 0.15 cm à 2.55 ± 0.18 cm, en une période de 4 semaines. Ceci est la première mention non équivoque d'une résistance systémique induite chez un conifère. Induzierte systemische Resistenz in Monterey-Kiefer Der pathogene Pilz Fusarium circinatum verursacht einen Krebs an Kiefern (pitch canker). Die vorliegende Untersuchung zeigt, dass die Monterey-Kiefer (Pinus radiata), eine der ökonomisch wichtigsten Kiefernarten der Welt und Hauptwirt in Kalifornien, auf Infektionen durch Fusarium circinatum mit induzierter systemischer Resistenz reagiert. Wiederholte mechanische Inokulationen der gleichen Bäume im Feld führten zu zunehmend kleineren Läsionen über einen Beobachtungszeitraum von zwei Jahren. Die Länge der Läsionen nahm von 2.89 ± 0.42 cm auf 1.04 ± 0.17 cm signifikant ab. Im Gewächshaus bewirkten prädisponierende Inokulationen mit dem Pathogen eine signifikante Reduktion der Läsionslänge von 5.5 ± 0.21 cm in den Kontrollen auf 4.46 ± 0.36 cm in den prädisponierten Pflanzen über einen Beobachtungszeitraum von sechs Wochen. Prädisponierende Inokulationen bewirkten auch unter konstanten Umweltbedingungen in einer Klimakammer eine signifikante Abnahme der Läsionsgrösse von 3.01 ± 0.15 cm auf 2.55 ± 0.18 über eine Periode von vier Wochen. Es handelt sich hier um den ersten eindeutigen Nachweis von induzierter systemischer Resistenz bei einer Konifere. [source] | ||||||||||||||||||||||