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Terms modified by Measured Selected AbstractsImmersive Integration of Physical and Virtual EnvironmentsCOMPUTER GRAPHICS FORUM, Issue 3 2004Henry Fuchs We envision future work and play environments in which the user's computing interface is more closely integrated with the physical surroundings than today's conventional computer display screens and keyboards. We are working toward realizable versions of such environments, in which multiple video projectors and digital cameras enable every visible surface to be both measured in 3D and used for display. If the 3D surface positions were transmitted to a distant location, they may also enable distant collaborations to become more like working in adjacent offices connected by large windows. With collaborators at the University of Pennsylvania, Brown University, Advanced Network and Services, and the Pittsburgh Supercomputing Center, we at Chapel Hill have been working to bring these ideas to reality. In one system, depth maps are calculated from streams of video images and the resulting 3D surface points are displayed to the user in head-tracked stereo. Among the applications we are pursuing for this tele-presence technology, is advanced training for trauma surgeons by immersive replay of recorded procedures. Other applications display onto physical objects, to allow more natural interaction with them "painting" a dollhouse, for example. More generally, we hope to demonstrate that the principal interface of a future computing environment need not be limited to a screen the size of one or two sheets of paper. Just as a useful physical environment is all around us, so too can the increasingly ubiquitous computing environment be all around us -integrated seamlessly with our physical surroundings. [source] A New Approach for Health Monitoring of Structures: Terrestrial Laser ScanningCOMPUTER-AIDED CIVIL AND INFRASTRUCTURE ENGINEERING, Issue 1 2007H. S. Park Three-dimensional (3D) coordinates of a target structure acquired using TLS can have maximum errors of about 10 mm, which is insufficient for the purpose of health monitoring of structures. A displacement measurement model is presented to improve the accuracy of the measurement. The model is tested experimentally on a simply supported steel beam. Measurements were made using three different techniques: (1) linear variable displacement transducers (LVDTs), (2) electric strain gages, and (3) a long gage fiber optic sensor. The maximum deflections estimated by the TLS model are less than 1 mm and within 1.6% of those measured directly by LVDT. Although GPS methods allow measurement of displacements only at the GPS receiver antenna location, the proposed TLS method allows measurement of the entire building's or bridge's deformed shape, and thus a realistic solution for monitoring structures at both structure and member level. Furthermore, it can be used to create a 3D finite element model of a structural member or the entire structure at any instance of time automatically. Through periodic measurements of deformations of a structure or a structural member and performing inverse structural analyses with the measured 3D displacements, the health of the structure can be monitored continuously. [source] Evaluation of rHA labeled with Gd,DTPA for blood pool imaging and targeted contrast deliveryCONTRAST MEDIA & MOLECULAR IMAGING, Issue 1 2010Jim M. Wild Abstract A new contrast agent was developed by linking Gd,DTPA chelate to recombinant human albumin in the laboratory. The molar relaxivity of the new agent was tested in aqueous solution at B0 1.5,T and temperature 20°C. The soluble compound had a higher molar longitudinal relaxivity and molar transverse relaxivity in water (r1,=,7.2,s,1,mM,1, r2,=,18.4,s,1,mM,1) than those measured for Gd,DTPA solution (r1,=,3.5,s,1,mM,1, r2,=,5.5,s,1,mM,1). The performance of the compound as a blood pool agent was investigated with soluble and microparticulate forms of the compound and comparisons were made with Gd,DTPA and the polymeric blood-pool agent, Gadomer. T1 -weighted imaging experiments show that the soluble compound acts as a highly effective blood pool agent with hyperintensity in the vasculature persisting beyond 2,h post administration, compared with free Gd,DTPA, which was cleared from the blood pool after approximately 10,min. The clearance kinetics of the new agents were examined, due to the incomplete elimination within 14 days post injection; both rHA labeled compounds are probably not suitable for development as routine blood pool contrast media. However, with free sites on the Gd-loaded rHA molecule, there are possibilities for binding the agent to antibodies in the laboratory, which was demonstrated, and thus there exist potential applications for in vivo molecular imaging with this agent. Copyright © 2010 John Wiley & Sons, Ltd. [source] In situ observation experiment for semiconductor solution growth under reduced convection condition , a reviewCRYSTAL RESEARCH AND TECHNOLOGY, Issue 7-8 2003Yuko Inatomi Abstract Morphological stability of solid/liquid interface in semiconductor crystal growth from solution has been investigated using a near-infrared microscopic interferometer under a reduced convection condition by authors and Prof. Benz's group. In the result, step kinetic coefficient of the interface of GaP/GaP(111)B in liquid phase epitaxy growth was obtained and the estimated value of macrostep wavelength agreed well with the measured one. [source] Damage identification of structures with uncertain frequency and mode shape dataEARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 5 2002Yong Xia Abstract A statistical method with combined uncertain frequency and mode shape data for structural damage identification is proposed. By comparing the measured vibration data before damage or analytical finite element model of the intact structure with those measured after damage, the finite element model is updated so that its vibration characteristic changes are equal to the changes in the measured data as closely as possible. The effects of uncertainties in both the measured vibration data and finite element model are considered as random variables in model updating. The statistical variations of the updated finite element model are derived with perturbation method and Monte Carlo technique. The probabilities of damage existence in the structural members are then defined. The proposed method is applied to a laboratory tested steel cantilever beam and frame structure. The results show that all the damages are identified correctly with high probabilities of damage existence. Discussions are also made on the applicability of the method when no measurement data of intact structure are available. Copyright © 2002 John Wiley & Sons, Ltd. [source] Metoprolol CR/XL Improves Systolic and Diastolic Left Ventricular Function in Patients with Chronic Heart FailureECHOCARDIOGRAPHY, Issue 3 2004Torstein Hole M.D. Aims: To investigate whether metoprolol controlled release/extended release (CR/XL) once daily would improve diastolic and systolic left ventricular function in patients with chronic heart failure and decreased ejection fraction. Methods: In an echocardiographic substudy to the Metoprolol CR/XL Randomized Intervention Trial in Heart Failure (MERIT-HF), 66 patients were examined three times during a 12-month period blinded to treatment group, assessing left ventricular dimensions and ejection fraction, and Doppler mitral inflow parameters, all measured in a core laboratory. Results: In the metoprolol CR/XL group left ventricular ejection fraction increased from 0.26 to 0.31 (P = 0.009) after a mean observation period of 10.6 months, and deceleration time of the early mitral filling wave (E) increased from 189 to 246 ms (P = 0.0012), time velocity integral of E-wave increased from 8.7 to 11.2 cm (P = 0.018), and the duration of the late mitral filling wave (A) increased from 122 to 145 ms (P = 0.014). No significant changes were seen in the placebo group regarding any of these variables. Conclusion: Metoprolol CR/XL once daily in addition to standard therapy improved both diastolic and systolic function in patients with chronic heart failure and decreased ejection fraction. (ECHOCARDIOGRAPHY, Volume 21, April 2004) [source] Habitat characteristics at bluegill spawning colonies in a South Dakota glacial lakeECOLOGY OF FRESHWATER FISH, Issue 4 2006N. J. C. Gosch Abstract,,, Bluegill (Lepomis macrochirus) primarily reproduce in spawning colonies. We assessed habitat characteristics at 15 bluegill spawning colonies in a South Dakota glacial lake. Nesting sites were visually identified and angling was used to verify the species of nesting fish. Habitat characteristics were measured at each nesting site and compared with those measured at 75 randomly selected sites. In Lake Cochrane, mean water depth of spawning colonies was 1.0 m. Of the 13 habitat characteristics measured, four (substrate type, substrate firmness, vegetation density and dissolved oxygen levels) were significantly different (P , 0.05) between nesting and random sites. Every bluegill nest site contained gravel substrate, despite the availability of muck, sand and rock. Substrate firmness was indexed at 0-cm penetration and vegetation density was low at all nesting sites. Additionally, bluegills selected nesting locations with relatively moderate dissolved oxygen levels. Lake Cochrane bluegill nest sites consisted of shallow, gravel areas with short, low-density, live submergent Chara vegetation. [source] A method to determine direct- and quadrature-axis inductances of permanent magnet synchronous motorsELECTRICAL ENGINEERING IN JAPAN, Issue 3 2010Shu Yamamoto Abstract The equivalent circuit constants of permanent magnet synchronous motors are needed in the calculation of operation characteristics, construction of a control system, etc. These constants can be computed from the data on structural form and materials. However, measurements are necessary to obtain highly precise values. Methods for measurement of the d- and q-axis inductances can be roughly divided into rotational and standstill methods. The standstill methods have the advantage that they are easy to carry out. However, it is difficult to consider magnetic saturation and distortion of the change in the armature winding inductance. The accuracy of the standstill method can be improved if these effects can be readily taken into account. This paper describes a standstill method for measuring accurate d- and q-axis synchronous inductances of permanent magnet synchronous motors. By utilizing the fact that the EMF interference terms in the motor voltage equation considering the distortion of the inductance change are equal to zero when the rotor is in a specific position, the proposed method determines the inductances considering both magnetic saturation and inductance distortion effects from simple off-line standstill testing. In addition, this method is capable of taking cross-magnetic saturation into account when used with the necessary testing equipment. The proposed method was implemented on a 0.4-kW interior permanent magnet synchronous motor with concentrated stator winding. The validity of the proposed method was demonstrated by comparing the measured and calculated results of the no-load and on-load characteristics. © 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 171(3): 41,50, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20969 [source] The active methanotrophic community in hydromorphic soils changes in response to changing methane concentrationENVIRONMENTAL MICROBIOLOGY, Issue 2 2006Claudia Knief Summary Methanotrophic communities were studied in several periodically water-saturated gleyic soils. When sampled, each soil had an oxic upper layer and consumed methane from the atmosphere (at 1.75 ppmv). In most gleyic soils the Km(app) values for methane were between 70 and 800 ppmv. These are higher than most values observed in dry upland soils, but lower than those measured in wetlands. Based on cultivation-independent retrieval of the pmoA -gene and quantification of partial pmoA gene sequences, type II (Alphaproteobacteria) methanotrophs of the genus Methylocystis spp. were abundant (> 107pmoA target molecules per gram of dry soil). Type I (Gammaproteobacteria) methanotrophs related to the genera Methylobacter and Methylocaldum/Methylococcus were detected in some soils. Six pmoA sequence types not closely related to sequences from cultivated methanotrophs were detected as well, indicating that diverse uncultivated methanotrophs were present. Three Gleysols were incubated under different mixing ratios of 13C-labelled methane to examine 13C incorporation into phospholipid fatty acids (PLFAs). Phospholipid fatty acids typical of type II methanotrophs, 16:0 and 18:1,7c, were labelled with 13C in all soils after incubation under an atmosphere containing 30 ppmv of methane. Incubation under 500 ppmv of methane resulted in labelling of additional PLFAs besides 16:0 and 18:1,7c, suggesting that the composition of the active methanotrophic community changed in response to increased methane supply. In two soils, 16:1 PLFAs typical of type I methanotrophs were strongly labelled after incubation under the high methane mixing ratio only. Type II methanotrophs are most likely responsible for atmospheric methane uptake in these soils, while type I methanotrophs become active when methane is produced in the soil. [source] Polybrominated diphenyl ethers, toxaphenes, and other halogenated organic pollutants in great blue heron eggsENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 2 2010Louise Champoux Abstract The great blue heron (Ardea herodias) has been used as a bioindicator of the state of the St. Lawrence River (Québec, Canada) since 1996. At 5-year intervals, selected breeding colonies along the River and its estuary are visited to estimate reproductive success and determine levels of contamination. Brominated flame retardants are found in many ecosystems and are increasing in concentration in the Great Lakes, which is the source of much of the water for the St. Lawrence River. In 2001 and 2002, in addition to polychlorinated biphenyls (PCBs) and chlorinated pesticides, the levels of polybrominated diphenyl ethers (PBDEs), polychlorinated bornanes (toxaphene) congeners and non- ortho -substituted PCBs were measured for the first time in pools of great blue heron eggs. The PBDE levels in great blue heron eggs (70,1,377,ng/g wet wt) were comparable to those measured in herring gull (Larus argentatus) eggs from the Great Lakes. Toxaphene was detected in great blue heron eggs at levels comparable to those of other major chlorinated pesticides. Major toxaphene congeners were octachlorobornane P44 and the nonachlorobornane P50. Environ. Toxicol. Chem. 2010;29:243,249. © 2009 SETAC [source] Trout density and health in a stream with variable water temperatures and trace element concentrations: Does a cold-water source attract trout to increased metal exposure?ENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 4 2009David D. Harper Abstract A history of hard-rock mining has resulted in elevated concentrations of heavy metals in Prickly Pear Creek (MT, USA). Remediation has improved water quality; however, dissolved zinc and cadmium concentrations still exceed U.S. Environmental Protection Agency water-quality criteria. Physical habitat, salmonid density, fish health, and water quality were assessed, and metal concentrations in fish tissues, biofilm, and macroinvertebrates were determined to evaluate the existing condition in the watershed. Cadmium, zinc, and lead concentrations in fish tissues, biofilm, and invertebrates were significantly greater than those at the upstream reference site and an experimental site farther downstream of the confluence. Fish densities were greatest, and habitat quality for trout was better, downstream of the confluence, where water temperatures were relatively cool (16°C). Measures of fish health (tissue metal residues, histology, metallothionein concentrations, and necropsies), however, indicate that the health of trout at this site was negatively affected. Trout were in colder but more contaminated water and were subjected to increased trace element exposures and associated health effects. Maximum water temperatures in Prickly Pear Creek were significantly lower directly below Spring Creek (16°C) compared to those at an experimental site 10 km downstream (26°C). Trout will avoid dissolved metals at concentrations below those measured in Prickly Pear Creek; however, our results suggest that the preference of trout to use cool water temperatures may supersede behaviors to avoid heavy metals. [source] Water-column concentrations and partitioning of polybrominated diphenyl ethers in the New York/New Jersey Harbor, USAENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 8 2008Archil Zarnadze Abstract Despite the emerging concern regarding polybrominated diphenyl ethers (BDEs), very few measurements of BDE concentrations in ambient water have been published. In the present study, BDEs were measured in water samples from the New York/New Jersey Harbor (USA). Samples were taken in Raritan Bay west of Sandy Hook during four intensive sampling campaigns in 2000 and 2001. Congeners 17, 47, 99, 100, 153, 154, 183, and 209 were detected. Total BDE (,BDE) concentrations (average ± standard deviation) were 175 ± 75 ng/g in the particle phase and 110 ± 72 pg/L in the apparent dissolved phase. The deca-congener, BDE 209, constituted 85 and 9% of ,BDEs in the particle and apparent dissolved phases, respectively. The ,BDE levels are significantly higher than those measured in Lake Ontario, USA, and in The Netherlands, but they are similar to concentrations measured in Lake Michigan and San Francisco Bay (both USA). Calculated values of the organic carbon-water partition coefficient (KOC) were strongly correlated with literature values of the octanol-water partition coefficient (KOW). The data suggest that sorption of BDEs to colloids is important in this system, although quantifying the extent of colloid sorption is difficult. [source] Inhibition of aquatic toxicity of pyrethroid insecticides by suspended sedimentENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 7 2006Weichun Yang Abstract The use of pyrethroid insecticides is increasing in both agricultural and urban environments. Although pyrethroids display very high acute toxicities to water column organisms in laboratory tests, environmental water samples typically contain suspended sediment (SS) that can reduce the freely dissolved concentration of pyrethroids, hence their bioavailability. Consequently, phase distribution could play an important role in pyrethroid aquatic toxicology. In this study, we evaluated the effect of SS on the acute toxicity of four widely used pyrethroid insecticides to Ceriodaphnia dubia. In all assays, median lethal concentrations (LC50s) consistently increased with increasing SS, demonstrating the pronounced inhibitory effects of SS on pyrethroid toxicity. The LC50s in the 200 mg/L SS solutions were 2.5 to 13 times greater than those measured in sediment-free controls. Solid-phase microextraction (SPME) was used to determine the apparent distribution coefficient Kd for the pyrethroids in the water samples. Under the assumption that only the freely dissolved fraction is bioavailable, the measured Kd was used to predict C. dubia LC50s in the water samples. The predicted LC50s were within a factor of two of the measured values for 95% of the treatments. Results from this study suggest that the inhibitory effect of SS can be highly significant and must be considered in estimating exposures to pyrethroids in aquatic systems. The SPME methodology could be used effectively to measure bioavailable concentration and to predict the actual ecotoxicologic effects of pyrethroids. [source] The efficacy of dantrolene sodium in controlling exertional rhabdomyolysis in the Thoroughbred racehorseEQUINE VETERINARY JOURNAL, Issue 7 2003J. G. T. Edwards Summary Reasons for performing study: Dantrolene sodium (Dantrium) has been used extensively for the treatment of myopathies in man and anecdotal evidence suggests it is of clinical benefit in the control of exercise-induced rhabdomyolysis (ER) in racehorses, although data to support this are currently lacking. Objectives: To investigate the efficacy of oral dantrolene sodium in controlling ER in a randomised, double-blind, placebo-controlled crossover trial involving 77 Thoroughbred racehorses in Newmarket, UK. Methods: Horses were treated on 2 occasions 1 week apart, with treatment days coinciding with a return to exercise following 2 days box rest on each occasion. For the first treatment, each horse was randomly selected to receive either 800 mg dantrolene sodium or a colour- matched placebo administered orally 1 h before exercise. This was followed by crossover to the other treatment on the second occasion, with each horse thereby acting as its own control. Degree of ER was assessed using rising serum creatine kinase (CK) levels, by subtracting pre-exercise blood CK levels from those measured in 6 h post exercise blood samples. For each horse, the difference in change between pre- and post exercise CK values between placebo and dantrolene treatments was calculated, with positive values indicating a greater rise with placebo than with dantrolene sodium treatment. Results: The overall mean difference for all horses was +104.8 iu/l and the null hypothesis, that there was no true difference in non-normally distributed post exercise rises in CK values between placebo and dantrolene treatments, was rejected (P = 0.0013) using the nonparametric Wilcoxon signed rank test. Additionally, no horses given dantrolene sodium showed clinical signs of ER, whereas 3 horses given the placebo developed ER following exercise. The incidence of ER in the study was 4% (3/77). Conclusions: The results confirmed that oral administration of dantrolene sodium, 1 h before exercise, had a statistically significant effect on reducing the difference between pre - and post exercise plasma CK levels compared with a placebo in the same animals, and preventing clinical ER in susceptible individuals. Potential relevance: This study suggested that dantrolene sodium is of use in controlling ER in the Thoroughbred racehorse. Further investigation into pre- and post exercise myoplasmic calcium levels and the repeat of the study late in the season when horses receive a much higher energy ration and more strenuous exercise would appear to be warranted. [source] Interventions to improve adherence to medication in people with type 2 diabetes mellitus: the role of nursesEUROPEAN DIABETES NURSING, Issue 2 2006Deputy Director, H Hearnshaw BSc, PhD Reader in Primary Care Abstract Summary Nurses now provide the majority of education and support for people with diabetes both in community and hospital settings. However, there are very few studies on nurse-led interventions to improve adherence to medication, a crucial element of the self-management of diabetes. The four studies reviewed formed a subgroup of a Cochrane review on interventions to improve adherence to medication in people with type 2 diabetes. Search terms were ,type 2 diabetes mellitus' and ,compliance' or ,adherence'. Studies were included if they assessed adherence to medical treatment specifically, rather than other aspects of self-management. Out of the 21 studies selected for review, four described an intervention delivered by a nurse. All four studies were from the USA and used an intervention delivered by telephone. Different interventions (two educational programmes, one automated telephone management system, one tracking system for health service and medication use) were backed up by a scripted nurse call. While patients in two studies reported improvements in self-care behaviour, only one measured a significant improvement in blood glucose control. Although some studies asked patients to report on their adherence to medication taking, responses from patients were not explicitly presented. The studies reviewed show the potential for generating evidence for the effectiveness of nurse-led diabetes management programmes. Further high-quality studies into this area are desperately needed, and they should consider new ways of evaluating complex interventions to generate more evidence. Copyright © 2006 FEND. [source] Brain natriuretic peptide in the prediction of recurrence of angina pectorisEUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Issue 2 2004H. Takase Abstract Background, Circulating levels of brain natriuretic peptide (BNP) provide prognostic information for patients with heart failure, but little is known about its prognostic usefulness in patients with stable angina pectoris. We investigated whether BNP could be used as a marker for the prediction of anginal recurrence after successful treatment. Design, Brain natriuretic peptide levels of 77 patients with stable angina pectoris were measured at enrolment and after confirmation of successful treatment (i.e. no anginal attack for at least 6 months: chronic phase) with percutaneous transluminal coronary angioplasty and/or conventional medication. Then, we prospectively followed them up for 25·9 ± 1·4 months, with the endpoint being a recurrence of anginal attacks. Results, An anginal attack recurred in seven patients. In patients without recurrence, BNP levels in the chronic phase (21 ± 12 [median ± median absolute deviation] pg mL,1) were lower than those measured at enrolment (46 ± 25 pg mL,1, P < 0·0001), whereas the levels in patients with recurrence increased during the same period (from 36 ± 16 to 72 ± 42 pg mL,1, P < 0·05). A univariate analysis revealed that the BNP level measured in the chronic phase was the significant predictor of future anginal recurrence. Analysis of the receiver operating characteristic curve indicated that the cutoff level of BNP in the chronic phase was 68 pg mL,1. The Kaplan-Meier method revealed that the incidence of anginal recurrence was higher in patients with higher (71·4%) than lower levels of BNP (2·9%; P < 0·0001). Conclusions, Measurement of BNP levels after successful therapy is clinically useful for the prediction of recurrence of anginal attacks in patients with angina pectoris. [source] Haemorheology in Gaucher diseaseEUROPEAN JOURNAL OF HAEMATOLOGY, Issue 3 2005Bridget E. Bax Abstract:, In Gaucher disease, a deficiency of glucocerebrosidase results in the accumulation of glucocerebroside within the lysosomes of the monocyte,macrophage system. Prior to the availability of enzyme replacement therapy (ERT), splenectomy was often indicated for hypersplenism. Haemorheological abnormalities could be expected in view of the anaemia and abnormal lipid metabolism in these patients and the role of the spleen in controlling erythrocyte quality. Objectives: To investigate the effect of Gaucher disease on blood and plasma viscosity, erythrocyte aggregation and erythrocyte deformability, and to determine whether observed rheological differences could be attributed to splenectomy. Methods: Haematological and haemorheological measurements were made on blood collected from 26 spleen-intact patients with Gaucher disease, 16 splenectomised patients with Gaucher disease, 6 otherwise healthy asplenic non-Gaucher disease subjects and 15 healthy controls. Results: No haemorheological differences could be demonstrated between spleen-intact patients with Gaucher disease and the control group. Compared to controls, both asplenic Gaucher disease and asplenic non-Gaucher disease study groups had a reduced MCHC (P = 0.003 and 0.005, respectively) and increased whole blood viscosity at 45% haematocrit (Hct), relative viscosity and red cell aggregation index , all measured at low shear (P < 0.05 for all). Additionally, asplenic patients with Gaucher disease alone showed an increased MCV (P = 0.006), an increased whole blood viscosity at 45% Hct measured at high shear (P = 0.019), and a reduced relative filtration rate (P = 0.0001), compared to controls. Conclusion: These observations demonstrate a direct and measurable haemorheological abnormality in Gaucher disease only revealed when there is no functioning spleen to control erythrocyte quality. [source] Estimation of net nitrogen flux between the atmosphere and a semi-natural grassland ecosystem in HungaryEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 5 2010A. Machon The aim of this work is to estimate the net N balance (deposition , emission) between the atmosphere and a semi-arid, semi-natural grassland (Bugac station, Central Hungary, CarboEurope IP, NitroEurope IP level 3 site). Dry deposition of N compounds has been determined by the inferential method, based on continuous monitoring of NO2 gas and daily 24-hour concentration measurements of HNO3 vapour, NH3 gas, and NH4+ and NO3, particles, using dry deposition velocities from the literature, measured above surfaces with the same characteristics as Bugac station. The bi-directional flux of NH3 within the atmosphere and the canopy (excluding soil emission) has also been estimated by the inferential method. Wet deposition of nitrate and ammonium ions was calculated on the basis of daily precipitation sampling and concentration measurements of nitrate and ammonium ions. To estimate the soil-atmosphere exchange of different gaseous N forms (N2, NO, N2O, NH3), the DNDC model was used as validated by the chamber measurements of NO and N2O soil emission fluxes. Soil emissions of NO and N2O have been determined by dynamic and static soil chamber methods, respectively. The measurement and modelling activity covers a complete year. Using the measured and modelled data, the calculated N balance at Bugac station between August 2006 and July 2007 is estimated at ,8.8 kg N ha,1 year,1 (deposition) as a sum of the deposition and emission terms (,10.4 and 1.6 kg N ha,1 year,1, respectively). Due to the warm and dry weather during the examined period, wet fluxes were substantially lower than usual, which may also have altered the regular yearly course of dry deposition and emission. [source] Estimating diesel degradation rates from N2, O2 and CO2 concentration versus depth data in a loamy sandEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 1 2007J. Van De Steene Summary The degradation rate of the pollutant is often an important parameter for designing and maintaining an active treatment system or for determining the rate of natural attenuation. A quasi-steady-state gas transport model based on Fick's law with a correction term for advective flux, for estimating diesel degradation rates from N2, O2 and CO2 concentration versus depth data, was evaluated in a laboratory column study. A loamy sand was spiked with diesel fuel at 0, 1000, 5000 and 10 000 mg kg,1 soil (dry weight basis) and incubated for 15 weeks. Soil gas was sampled weekly at 6 selected depths in the columns and analysed for O2, CO2 and N2 concentrations. The agreement between the measured and the modelled concentrations was good for the untreated soil (R2= 0.60) and very good for the soil spiked with 1000 mg kg,1 (R2= 0.96) and 5000 mg kg,1 (R2= 0.97). Oxygen consumption ranged from ,0.15 to ,2.25 mol O2 m,3 soil day,1 and CO2 production ranged from 0.20 to 2.07 mol CO2 m,3 soil day,1. A significantly greater mean O2 consumption (P < 0.001) and CO2 production (P < 0.005) over time was observed for the soils spiked with diesel compared with the untreated soil, which suggests biodegradation of the diesel substrate. Diesel degradation rates calculated from respiration data were 1.5,2.1 times less than the change in total petroleum hydrocarbon content. The inability of this study to correlate respiration data to actual changes in diesel concentration could be explained by volatilization, long-term sorption of diesel hydrocarbons to organic matter and incorporation of diesel hydrocarbons into microbial biomass, aspects of which require further investigation. [source] The role of mineral and organic components in phenanthrene and dibenzofuran sorption by soilEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 3 2006R. Celis Summary Improved predictions of sorption of hydrophobic organic compounds (HOCs) in soil require a better knowledge of the relative contribution of inorganic and organic soil constituents to the sorption process. In this paper, sorption of a three-ring polycyclic aromatic hydrocarbon (phenanthrene) and a three-ring heterocyclic,aromatic compound (dibenzofuran) by six agricultural soils, their clay-size fractions, and a series of single, binary, and ternary model sorbents was evaluated to elucidate the relative role of soil mineral and organic components in the retention of these two model HOCs. The sorption coefficients for phenanthrene and dibenzofuran on purified soil organic materials (Kd = 821,9080 litre kg,1) were two orders of magnitude greater than those measured on mineral model sorbents (Kd = 0,114 litre kg,1). This, along with the strong correlation between sorption and the organic C content of the soil clay fractions (r = 0.99, P < 0.01), indicated a primary role of soil organic matter in the retention of both compounds. However, weak relationships between phenanthrene and dibenzofuran sorption coefficients and the organic C content of the bulk soils and variability of Koc values among soils, clay fractions, and model sorbents (1340,21020 litre kg,1 C for phenanthrene and 1685,7620 litre kg,1 C for dibenzofuran) showed that sorption was not predictable exclusively from the organic C content of the materials. Organic matter heterogeneity and domain blockage arising from organic matter,clay interactions and associated pH shifts were identified as the most likely causes of the different organic C-normalized sorption capacities of the soils. A direct contribution from minerals to the sorption of phenanthrene and dibenzofuran by the soils studied was likely to be small. Our results suggested that suitable descriptors for the extent of organic matter,mineral interactions would help to improve current Koc -based sorption predictions and subsequently the assessment of risk associated with the presence of HOCs in soil. [source] Effect of temperature and moisture on rates of carbon mineralization in a Mediterranean oak forest soil under controlled and field conditionsEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 5 2005A. Rey Summary We examined the relationship between carbon mineralization (Cmin), moisture and temperature in a Mediterranean forest soil under controlled and field conditions. We studied the following. 1,The temperature sensitivity at three soil depths: soil samples were incubated at 4, 10, 20 and 30°C at optimal water content of 60% of water holding capacity (WHC). Values of Cmin of the top layer were more than 12 times faster than those measured in deeper layers. We found a temperature sensitivity factor (Q10) of 3.3, 2.7 and 2.2 for the 0,5 cm, 5,10 cm and 10,20 cm layers, respectively. 2,The relationship between Cmin, moisture and temperature (top layer). The sensitivity of Cmin to fluctuating moisture depended on temperature. However, the Q10 was not significantly affected by soil moisture. We fitted a multiple polynomial model that predicted Cmin as a multiplicative function of temperature and moisture (R2 > 0.99). 3,The response of Cmin of soil to rewetting after 1 and 24 hours. In all cases, the response was rapid. The soil incubated at 60% WHC or less responded positively to a sudden increase in water content, with the largest increase in the 20% WHC treatment. The model predicted Cmin in the field well when rewetting effects were taken into account (R2 > 0.81). These results indicate that sudden changes in soil moisture can lead to increased carbon mineralization during the dry summer. It is necessary to include such responses in models as they may represent a substantial loss of carbon in the overall carbon balance of Mediterranean ecosystems. [source] Fluorescence induction of protoporphyrin IX by a new 5-aminolevulinic acid nanoemulsion used for photodynamic therapy in a full-thickness ex vivo skin modelEXPERIMENTAL DERMATOLOGY, Issue 8 2010Tim Maisch Please cite this paper as: Fluorescence induction of protoporphyrin IX by a new 5-aminolevulinic acid nanoemulsion used for photodynamic therapy in a full-thickness ex vivo skin model. Experimental Dermatology 2010; 19: e302,e305. Abstract:, An ex vivo porcine skin model was utilized to analyse the penetration of 5-aminolevulinic acid (5-ALA) contained in a nanoemulsion-based formulation BF-200 ALA (10% 5-ALA-hydrochloride) versus 16% aminolevulinate methyl ester-hydrochloride in a commercially cream (MAL cream) by fluorescence microscopy of their common metabolite protoporphyrin IX (PpIX) after 3, 5, 8 and 12 h. Fluorescence signals of PpIX in pig skin treated with BF-200 ALA were stronger than those for MAL cream. At 8 and 12 h, the PpIX fluorescence signals were 4.8- and 5.0-fold higher than those measured after MAL cream application. Fluorescence signals of PpIX after application of BF-200 ALA were detected in deeper tissue layers of the epidermis than after application of MAL cream (97.2 ± 5.7 ,m for BF-200 ALA vs 42.0 ± 4.2 ,m for MAL cream). These data implicate that BF-200 ALA in photodynamic therapy might lead to a superior therapeutically effect of intraepidermal (in situ) squamous cell carcinomas. [source] Calcium homeostasis and signaling in yeast cells and cardiac myocytesFEMS YEAST RESEARCH, Issue 8 2009Jiangjun Cui Abstract Calcium ions are the most ubiquitous and versatile signaling molecules in eukaryotic cells. Calcium homeostasis and signaling systems are crucial for both the normal growth of the budding yeast Saccharomyces cerevisiae and the intricate working of the mammalian heart. In this paper, we make a detailed comparison between the calcium homeostasis/signaling networks in yeast cells and those in mammalian cardiac myocytes. This comparison covers not only the components, structure and function of the networks but also includes existing knowledge on the measured and simulated network dynamics using mathematical models. Surprisingly, most of the factors known in the yeast calcium homeostasis/signaling network are conserved and operate similarly in mammalian cells, including cardiac myocytes. Moreover, the budding yeast S. cerevisiae is a simple organism that affords powerful genetic and genomic tools. Thus, exploring and understanding the calcium homeostasis/signaling system in yeast can provide a shortcut to help understand calcium homeostasis/signaling systems in mammalian cardiac myocytes. In turn, this knowledge can be used to help treat relevant human diseases such as pathological cardiac hypertrophy and heart failure. [source] Numerical parameter study of the thermal behaviour of a gypsum plaster board at fire temperaturesFIRE AND MATERIALS, Issue 2 2008Luc Wullschleger Abstract Based on a comparison between the measured and the calculated temperature evolutions within a gypsum plaster board subject to fire, a numerical parameter study regarding the material properties of gypsum at elevated temperatures has been carried out. This allowed quantifying the individual influence of different quantities and their deviations on the calculated thermal behaviour of this material. Copyright © 2007 John Wiley & Sons, Ltd. [source] Application of a one-dimensional thermal flame spread model on predicting the rate of heat release in the SBI testFIRE AND MATERIALS, Issue 2 2001Tuula Hakkarainen A one-dimensional thermal flame spread model was applied to predict the rate of heat release in the single burning item (SBI) test on the basis of the cone calorimeter data. The input parameters were selected according to the features of the SBI test and using particle board as a model tuning material. The features of the measured and calculated rate of heat release curves were compared for a series of 33 building products. The fire growth rate (FIGRA) indices were calculated to predict the classification in the forthcoming Euroclass system. The model gave correct classification for 90% of the products studied. An essential feature of the model is that only one cone calorimeter test at the exposure level of 50 kW m,2 is needed. The model, therefore, provides a practical tool for product development and quality control. Copyright © 2001 John Wiley & Sons, Ltd. [source] Integration of lakes and streams in a landscape perspective: the importance of material processing on spatial patterns and temporal coherenceFRESHWATER BIOLOGY, Issue 3 2000George W. Kling 1. We studied the spatial and temporal patterns of change in a suite of twenty-one chemical and biological variables in a lake district in arctic Alaska, U.S.A. The study included fourteen stream sites and ten lake sites, nine of which were in a direct series of surface drainage. All twenty-four sites were sampled between one and five times a year from 1991 to 1997. 2. Stream sites tended to have higher values of major anions and cations than the lake sites, while the lake sites had higher values of particulate carbon, nitrogen, phosphorous and chlorophyll a. There were consistent and statistically significant differences in concentrations of variables measured at the inlet versus the outlet of lakes, and in variables measured at upstream versus downstream sites in the stream reaches which connect the lakes. In-lake processing tended to consume alkalinity, conductivity, H+, DIC, Ca2+, Mg2+, CO2, CH4, and NO3,, and produce K+ and dissolved organic carbon (DOC). In-stream processing resulted in the opposite trends (e.g. consumption of K+ and DOC), and the magnitudes of change were often similar to those measured in the lakes but with the opposite sign. 3. Observed spatial patterns in the study lakes included mean concentrations of variables which increased, decreased or were constant along the lake chain from high to low altitude in the catchment (stream sites showed no spatial patterns with any variables). The strongest spatial patterns were of increasing conductivity, Ca2+, Mg2+, alkalinity, dissolved inorganic carbon and pH with lake chain number (high to low altitude in the basin). These patterns were partly determined by the effect of increasing catchment area feeding into lakes further downslope, and partly by the systematic processing of materials in lakes and in the stream segments between lakes. 4. Synchrony (the temporal coherence or correlation of response) of variables across all lakes ranged from 0.18 for particulate phosphorus to 0.90 for Mg2+ the average synchrony for all twenty-one variables was 0.50. The synchronous behaviour of lake pairs was primarily related to the spatial location or proximity of the lakes for all variables taken together and for many individual variables, and secondarily, to the catchment to lake area ratio and the water residence time. 5. These results illustrate that, over small geographic areas, and somewhat independent of lake or stream morphometry, the consistent and directional (downslope) processing of materials helps produce spatial patterns which are coherent over time for many limnological variables. We combine concepts from stream, lake and landscape ecology, and develop a conceptual view of landscape mass balance. This view highlights that the integration of material processing in both lakes and rivers is critical for understanding the structure and function of surface waters, especially from a landscape perspective. [source] Dynamics of heat-induced thermal stress resistance and hsp70 expression in the springtail, Orchesella cinctaFUNCTIONAL ECOLOGY, Issue 2 2009Simon Bahrndorff Summary 1The relationship between thermal resistance and expression of inducible heat shock proteins, especially Hsp70, depends on the species and temperature treatments. The induction of Hsp70 has been shown to be essential for heat stress survival in a number of species, yet the maximum protein expression levels do not coincide with peak survival after heat hardening in Drosophila. 2Here we study the functional relationship between heat-induced expression of the heat shock protein Hsp70, and thermal resistance in adult Orchesella cincta by comparing thermal resistance (survival of 37·4 °C for 60 min) with Hsp70 gene and protein expression levels, all three measured at time points 2, 4, 6, 23, 27, 49 h after a heat hardening treatment (35·4 °C for 60 min). 3Thermotolerance increased over time after heat hardening until 49 h after exposure when the experiment ended. On the other hand the expression of hsp70 messenger RNA reached a peak within the first 2 h and then sharply decreased after 6 h. Within 23 h hsp70 expression was back to control levels. 4Surprisingly, protein levels of Hsp70 followed thermotolerance and reached the highest levels 49 h after heat hardening. A significant positive association was found between thermotolerance and Hsp70 protein levels, but not with hsp70 mRNA levels. 5Our results support a strong correlation between Hsp70 expression levels and thermal resistance following a heat hardening treatment. They also show that gene and protein expression follow different dynamics, a difference that may be important for our understanding of the role of candidate genes in functional studies. [source] Growth and physiological acclimation to temperature and inorganic carbon availability by two submerged aquatic macrophyte species, Callitriche cophocarpa and Elodea canadensisFUNCTIONAL ECOLOGY, Issue 2 2000B. Olesen Abstract 1.,Interactive effects of temperature and inorganic carbon availability on photosynthetic acclimation and growth of two submerged macrophyte species, Elodea canadensis and Callitriche cophocarpa, were examined to test the hypotheses that: (1) effects of temperature on growth rate and photosynthetic acclimation are suppressed under low inorganic carbon availability; (2) the plants compensate for the reduction in activity of individual enzymes at lower temperatures by increasing the activity per unit plant mass, here exemplified by Rubisco. The experiments were performed in the laboratory where plants were grown in a factorial combination of three temperatures (7,25 °C) and three inorganic carbon regimes. 2.,The relative growth rate of both species was strongly affected by growth conditions and increased by up to 4·5 times with increased temperature and inorganic carbon availability. The sensitivity to inorganic carbon was greatest at high temperature and the sensitivity to temperature greatest at high carbon concentrations. 3.,Photosynthetic acclimation occurred in response to growth conditions for both species. The affinity for inorganic carbon and the photosynthetic capacity, both measured at 15 °C, increased with reduced inorganic carbon availability during growth and were greater at warmer than at cooler growth temperature. The acclimative change in photosynthesis was related to the extent of temperature and inorganic carbon stress. Using data for Elodea, a negative relationship between degree of temperature stress and photosynthetic performance was found. In relation to inorganic carbon, a linear increase in CO2 affinity and photosynthetic capacity was found with increased inorganic carbon stress during growth. 4.,The total Rubisco activity declined with increased inorganic carbon availability during growth and with enhanced growth temperature. In addition, the activation state of Rubisco was higher at cooler than at warmer temperatures for Callitriche. This suggests that low-temperature grown plants compensate for the temperature-dependent reduction in activity of the individual Rubisco molecules by enhancing resource allocations towards Rubisco. [source] A geometric and kinematic model for double-edge propagating thrusts involving hangingwall and footwall folding.GEOLOGICAL JOURNAL, Issue 5-6 2010An example from the Jaca, Pamplona Basin (Southern Pyrenees) Abstract A new geometric and kinematic model is proposed for a particular type of fault-related folding based on the study of a natural example developed in Palaeogene carbonate rocks from the Jaca,Pamplona Basin (Southern Pyrenees). The example consists of a hangingwall anticline related to a reverse fault with variable displacement and a gentle footwall syncline. A detailed structural analysis of the structure and a cross-section, perpendicular to its axis and parallel to the transport direction, reveals that none of the previous published models of fault-related folds is able to simulate its main characteristics and reproduce its geometry. The main features of the new model are: double-edge propagating fault and folding developed in both the hangingwall and the footwall. A MATLAB-based program was created to calculate structural parameters such as shortening, structural relief and fault slip; obtain graphs of different parameters such as shortening versus slip along the fault, shortening versus fault length, and produce sections across forward models showing the different stages of fold growth. The model presented here gives an acceptable geometrical fit to the studied natural structure and provides a reasonable evolutionary history. In addition, the results obtained using the model are similar to those measured on the cross-section. As a final step the subsurface portion of the natural fold was completed following the constraints imposed by the model. Copyright © 2010 John Wiley & Sons, Ltd. [source] Soil organic carbon contents in long-term experimental grassland plots in the UK (Palace Leas and Park Grass) have not changed consistently in recent decadesGLOBAL CHANGE BIOLOGY, Issue 7 2009D. W. HOPKINS Abstract A recent report of widespread declines in soil organic C (SOC) in the UK over the 10,25 years until the early 2000s has focussed attention on the importance of resampling previously characterized sites to assess long-term trends in SOC contents and the importance of soils as a potentially volatile and globally significant reservoir of terrestrial C. We have used two sets of long-term experimental plots which have been under constant and known management for over a century and for which historical data exist that allow comparison over recent decades to determine what, if any, changes in SOC content have occurred. The plots used are the Palace Leas (PL) Meadow Hay Plots in north-east England (UK) established in 1897, and from the Park Grass (PG) Continuous Hay experiment established in 1856 at Rothamsted in south-east England. Collectively, these plots represent the only grassland sites in the UK under long-term management where changes in SOC over several decades can be assessed, and are probably unique in the world. The plots have received different manure and fertilizer treatment and have been under known management for at least 100 years. In 1982, total SOC contents were determined for the 0,27 cm layer of six of the PL plots using measurements of SOC concentrations, bulk density and soil depth. In 2006, the same six PL plots were resampled and SOC contents determined again. Four of the plots showed no net change in SOC content, but two plots showed net loss of SOC of 15% and 17% (amounting to decreases of 18 and 15 t C ha,1) since 1982. However, these differences in total SOC content were in a similar range to the variations in bulk density (6,31%) with changing soil water content. In 1959, the soil masses and SOC concentrations to 23 cm depth were measured on six PG plots with fertilizer and manure treatments corresponding closely with those measured on PL. In 2002, the SOC concentrations on the same plots were measured again. On three of the PG plots, SOC concentrations had declined by 2,10%, but in the other three it had increased by 4,8% between 1959 and 2002. If it is assumed that the soil bulk density had not changed over this period, the losses of SOC from the top soils ranged range from 10 to 3 t C ha,1, while the gains ranged from 4 to 7 t C ha,1. When the differences with time in SOC contents for the six PL and the six PG plots were examined using paired t -tests, that is, regarding the plots as two sets of six replicate permanent grasslands, there were no significant differences between 1982 and 2006 for the PL plots or between 1959 and 2002 for the PG plots. Thus, these independent observations on similar plots at PL and PG indicate there has been no consistent decrease in SOC stocks in surface soils under old, permanent grassland in England in recent decades, even though meteorological records for both sites indicate significant warming of the soil and air between 1980 and 2000. Because the potential influences of changes in management or land use have been definitively excluded, and measured rather than derived bulk densities have been used to convert from SOC concentrations to SOC amounts, our observations question whether for permanent grassland in England, losses in SOC in recent decades reported elsewhere can be attributed to widespread environmental change. [source] |