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Maritime Pine (maritime + pine)

Distribution by Scientific Domains
Life Sciences100%

Selected Abstracts

Range-wide phylogeography and gene zones in Pinus pinaster Ait. revealed by chloroplast microsatellite markers

MOLECULAR ECOLOGY, Issue 10 2007
GABRIELE BUCCI
Abstract Some 1339 trees from 48 Pinus pinaster stands were characterized by five chloroplast microsatellites, detecting a total of 103 distinct haplotypes. Frequencies for the 16 most abundant haplotypes (pk > 0.01) were spatially interpolated over a lattice made by 430 grid points. Fitting of spatially interpolated values on raw haplotype frequencies at the same geographical location was tested by regression analysis. A range-wide ,diversity map' based on interpolated haplotype frequencies allowed the identification of one ,hotspot' of diversity in central and southeastern Spain, and two areas of low haplotypic diversity located in the western Iberian peninsula and Morocco. Principal component analysis (PCA) carried out on haplotypes frequency surfaces allowed the construction of a colour-based ,synthetic' map of the first three PC components, enabling the detection of the main range-scale genetic trends and the identification of three main ,gene pools' for the species: (i) a ,southeastern' gene pool, including southeastern France, Italy, Corsica, Sardinia, Pantelleria and northern Africa; (ii) an ,Atlantic' gene pool, including all the western areas of the Iberian peninsula; and (iii) a ,central' gene pool, located in southeastern Spain. Multivariate and amova analyses carried out on interpolated grid point frequency values revealed the existence of eight major clusters (,gene zones'), whose genetic relationships were related with the history of the species. In addition, demographic models showed more ancient expansions in the eastern and southern ranges of maritime pine probably associated to early postglacial recolonization. The delineation of the gene zones provides a baseline for designing conservation areas in this key Mediterranean pine. [source]

Effective gene dispersal and female reproductive success in Mediterranean maritime pine (Pinus pinaster Aiton)

MOLECULAR ECOLOGY, Issue 14 2006
SANTIAGO C. GONZÁLEZ-MARTÍNEZ
Abstract Understanding population-scale processes that affect allele frequency changes across generations is a long-standing interest in genetic, ecological and evolutionary research. In particular, individual differences in female reproductive success and the spatial scale of gene flow considerably affect evolutionary change and patterns of local selection. In this study, a recently developed maximum-likelihood (ML) method based on established offspring, the Seedling Neighbourhood Model, was applied and exponentially shaped dispersal kernels were fitted to both genetic and ecological data in a widespread Mediterranean pine, Pinus pinaster Aiton. The distribution of female reproductive success in P. pinaster was very skewed (about 10% of trees mothered 50% of offspring) and significant positive female selection gradients for diameter (, = 0.7293) and cone crop (, = 0.4524) were found. The selective advantage of offspring mothered by bigger trees could be due to better-quality seeds. These seeds may show more resilience to severe summer droughts and microsite variation related to water and nutrient availability. Both approaches, ecological and of parentage, consistently showed a long-distance dispersal component in saplings that was not found in dispersal kernels based on seed shadows, highlighting the importance of Janzen-Connell effects and microenvironmental variation for survival at early stages of establishment in this Mediterranean key forest tree. [source]

Comparison of genetic diversity estimates within and among populations of maritime pine using chloroplast simple-sequence repeat and amplified fragment length polymorphism data

MOLECULAR ECOLOGY, Issue 5 2002
M. M. Ribeiro
Abstract We compared the genetic variation of Pinus pinaster populations using amplified fragment length polymorphism (AFLP) and chloroplast simple-sequence repeat (cpSSR) loci. Populations' levels of diversity within groups were found to be similar with AFLPs, but not with cpSSRs. The high interlocus variance associated with the AFLP loci could account for the lack of differences in the former. Although AFLPs revealed much lower genetic diversity than cpSSRs, the levels of among-population differentiation found with the two types of marker were similar, provided that loci showing fewer than four null-homozygotes, in any population, were pruned from the AFLP data. Moreover, the French and Portuguese populations were clearly differentiated from each other, with both markers. The Mantel test showed that the genetic distance matrix calculated using the AFLP data was correlated with the matrix derived from the cpSSRs. Because of the concordance found between markers we conclude that gene flow was indeed the predominant force shaping nuclear and chloroplastic genetic variation of the populations within regions, at the geographical scale studied. [source]

Genetic parameters and QTL analysis of ,13C and ring width in maritime pine

PLANT CELL & ENVIRONMENT, Issue 8 2002
O. Brendel
Abstract Classical quantitative genetics and quantitative trait dissection analysis (QTL) approaches were used in order to investigate the genetic determinism of wood cellulose carbon isotope composition (,13C, a time integrated estimate of water use efficiency) and of diameter growth and their relationship on adult trees (15 years) of a forest tree species (maritime pine). A half diallel experimental set-up was used to (1) estimate heritabilities for ,13C and ring width and (2) to decompose the phenotypic ,13C/growth correlation into its genetic and environmental components. Considerable variation was found for ,13C (range of over 3,) and for ring width (range of over 5 mm) and significant heritabilities (narrow sense 0ˇ17/0ˇ19 for ,13C and ring width, respectively, 100% additivity). The significant phenotypic correlation between ,13C and ring width was not determined by the genetic component, but was attributable to environmental components. Using a genetic linkage map of a full-sib family, four significant and four suggestive QTLs were detected for ,13C, the first for ,13C in a forest tree species, as far as known to the authors. Two significant and four suggestive QTLs were found for ring width. No co-location of QTLs was found between ,13C and growth. [source]