Home About us Contact
|
Home About us Contact | ||
|
|
||
Many Members (many + member)
Selected AbstractsDistinct expression of C1q-like family mRNAs in mouse brain and biochemical characterization of their encoded proteinsEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 9 2010Takatoshi Iijima Abstract Many members of the C1q family, including complement C1q and adiponectin, and the structurally related tumor necrosis factor family are secreted and play crucial roles in intercellular signaling. Among them, the Cbln (precerebellin) and C1q-like (C1ql) subfamilies are highly and predominantly expressed in the central nervous system. Although the Cbln subfamily serve as essential trans-neuronal regulators of synaptic integrity in the cerebellum, the functions of the C1ql subfamily (C1ql1,C1ql4) remain unexplored. Here, we investigated the gene expression of the C1ql subfamily in the adult and developing mouse brain by reverse transcriptase-polymerase chain reaction and high-resolution in-situ hybridization. In the adult brain, C1ql1,C1ql3 mRNAs were mainly expressed in neurons but weak expression was seen in glia-like structures in the adult brain. The C1ql1 mRNA was predominantly expressed in the inferior olive, whereas the C1ql2 and C1ql3 mRNAs were strongly coexpressed in the dentate gyrus. Although the C1ql1 and C1ql3 mRNAs were detectable as early as embryonic day 13, the C1ql2 mRNA was observed at later embryonic stages. The C1ql1 mRNA was also expressed transiently in the external granular layer of the cerebellum. Biochemical characterization in heterologous cells revealed that all of the C1ql subfamily proteins were secreted and they formed both homomeric and heteromeric complexes. They also formed hexameric and higher-order complexes via their N-terminal cysteine residues. These results suggest that, like Cbln, the C1ql subfamily has distinct spatial and temporal expression patterns and may play diverse roles by forming homomeric and heteromeric complexes in the central nervous system. [source] The Uses of a Good TheoryJOURNAL OF SOCIAL ISSUES, Issue 2 2006Faye J. Crosby How does one diminish discrimination? Many members of SPSSI, including the present authors, have tried to reduce discrimination through the application of good theories. We outline three theoretical approaches that Crosby, like many other psychologists, has taken as she has struggled with discrimination. Sometimes missing in Crosby's approach, and often missing in the approach of others, is a frank avowal of values. We argue that the attempt to divorce science from values renders theories less effective than they need be and even allows unexamined values to contaminate good research. [source] Titian's three-altar project in the Venetian church of San Salvador: strategies of self-representation by members of the Scuola Grande di San RoccoRENAISSANCE STUDIES, Issue 4 2001D Bohde In the Venetian church of San Salvador there are two altarpieces by Titian, the Transfiguration and the Annunciation. A third altarpiece by him, a Crucifixion, was also planned, but never erected. Together they were supposed to display a christological programme. The paper argues that the three altarpieces were co-ordinated by Titian and the patrons of the two side altars. These patrons were non-noble merchants, involved like Titian in the Scuola Grande di San Rocco. Many members of this lay confraternity desired a tomb or an altar in San Salvador, an important church near the Rialto. A history of the commissions for these altars and tombs reveals how cittadini acted in an area traditionally dominated by patricians. Their strategy of adapting to Venetian traditions on the one hand, and of developing their own modes of representation on the other, is most evident in Titian's patrons. A closer examination of Titian's Annunciation demonstrates how the painter and the patron dealt with such a crucial theme for the myth of Venice. Titian affirmed the traditional iconography of the Annunziata, but provided it with highly innovative features: the open brushwork and the unusual physicality of the holy figures express the incarnation of the divine Word. [source] Crystallization and initial crystallographic characterization of a vicilin-type seed storage protein from Pinus koraiensisACTA CRYSTALLOGRAPHICA SECTION F (ELECTRONIC), Issue 12 2007Tengchuan Jin The cupin superfamily of proteins includes the 7S and 11S seed storage proteins. Many members of this family of proteins are known allergens. In this study, the Korean pine (Pinus koraiensis) vicilin-type 7S seed storage protein was isolated from defatted pine-nut extract and purified by sequential gel-filtration and anion-exchange chromatography. Well diffracting single crystals were obtained by the vapor-diffusion method in hanging drops. The crystals belong to the primitive cubic space group P213, with unit-cell parameters a = b = c = 148.174,Å. Two vicilin molecules were present in the asymmetric unit and the Matthews coefficient was determined to be 2.90,Å3,Da,1, with a corresponding solvent content of ,58%. A molecular-replacement structural solution has been obtained using the program Phaser. Refinement of the structure is currently under way. [source] Get it Off Your Chest: Contexts in Creative Community WorkingINTERNATIONAL JOURNAL OF ART & DESIGN EDUCATION, Issue 1 2003June Bianchi Three years ago I started work on the exhibition Get it off your chest, a multimedia project exploring the personal and social role of the breast within British culture. The project would involve over one hundred people as contributors, engaging with ongoing debates within academic, media and informal contexts as to what constitutes and impacts upon constructions of the female image within our society, particularly in relation to the breast as a primary signifier. The working practices evolved in creating Get it off your chest were instrumental in generating a synergy in my own creative activities, enabling some measure of unification to occur within the strands of my art,making and art educational roles. This synergistic approach, which I term ,creative community working' will be discussed in this paper alongside the epistemological focus of the exhibition, its inception and its consequent structure, presentation and wider educational role. I will focus throughout on exploring the development of creative community working contexts: the impulse to integrate what sometimes seem like rogue elements of the professional and creative identity is one shared by many members of the art educational community and I hope that this paper will generate feedback and discussion on the diverse ways in which colleagues generate synergy in their own working lives. [source] Assessment in the context of uncertainty: how many members are needed on the panel of reference of a script concordance test?MEDICAL EDUCATION, Issue 3 2005R Gagnon Purpose, The script concordance test (SCT) assesses clinical reasoning in the context of uncertainty. Because there is no single correct answer, scoring is based on a comparison of answers provided by examinees with those provided by members of a panel of reference made up of experienced practitioners. This study aims to determine how many members are needed on the panel to obtain reliable scores to compare against the scores of examinees. Methods, A group of 80 residents were tested on 73 items (Cronbach's ,: 0.76). A total of 38 family doctors made up the pool of experienced practitioners, from which 1000 random panels of reference of increasing sizes (5, 10, 15, 20, 25 and 30) were generated with a resampling procedure. Residents' scores were computed for each panel sample. Units of analysis were means of residents' score, test reliability coefficient and correlation coefficient between scores obtained with a given panel of reference versus the scores obtained with the full panel of 38. Statistics were averaged across the 1000 samples for each panel size for the mean and test reliability computations, and across 100 samples for the correlation computation. Results, For sample variability, there was a 3-fold increase in standard deviation of means between a sample panel size of 5 (SD = 1.57) and a panel size of 30 (SD = 0.50). For reliability, there was a large difference in precision between a panel size of 5 (0.62) and a panel size of 10 (0.70). When the panel size was over 20, the gain became negligible (0.74 for 20 and 0.76 for 38). For correlation, the mean correlation coefficient values were 0.90 with 5 panel members, 0.95 with 10 members and 0.98 with 20 members. Conclusion, Any number over 10 is associated with acceptable reliability and good correlation between the samples versus the full panel of 38. For high stake examinations, using a panel of 20 members is recommended. Recruiting more than 20 panel members shows only a marginal benefit in terms of psychometric properties. [source] Structure and Photoreaction of Photoactive Yellow Protein, a Structural Prototype of the PAS Domain Superfamily,PHOTOCHEMISTRY & PHOTOBIOLOGY, Issue 1 2007Yasushi Imamoto Photoactive yellow protein (PYP) is a water-soluble photosensor protein found in purple photosynthetic bacteria. Unlike bacterial rhodopsins, photosensor proteins composed of seven transmembrane helices and a retinal chromophore in halophilic archaebacteria, PYP is a highly soluble globular protein. The ,/, fold structure of PYP is a structural prototype of the PAS domain superfamily, many members of which function as sensors for various kinds of stimuli. To absorb a photon in the visible region, PYP has a p -coumaric acid chromophore binding to the cysteine residue via a thioester bond. It exists in a deprotonated trans form in the dark. The primary photochemical event is photo-isomerization of the chromophore from trans to cis form. The twisted cis chromophore in early intermediates is relaxed and finally protonated. Consequently, the chromophore becomes electrostatically neutral and rearrangement of the hydrogen-bonding network triggers overall structural change of the protein moiety, in which local conformational change around the chromophore is propagated to the N-terminal region. Thus, it is an ideal model for protein conformational changes that result in functional change, responding to stimuli and expressing physiological activity. In this paper, recent progress in investigation of the photoresponse of PYP is reviewed. [source] | ||||||||||