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Many Gram-negative Bacteria (many + gram-negative_bacteria)
Selected AbstractsThe F4 fimbrial chaperone FaeE is stable as a monomer that does not require self-capping of its pilin-interactive surfacesACTA CRYSTALLOGRAPHICA SECTION D, Issue 5 2009Inge Van Molle Many Gram-negative bacteria use the chaperone,usher pathway to express adhesive surface structures, such as fimbriae, in order to mediate attachment to host cells. Periplasmic chaperones are required to shuttle fimbrial subunits or pilins through the periplasmic space in an assembly-competent form. The chaperones cap the hydrophobic surface of the pilins through a donor-strand complementation mechanism. FaeE is the periplasmic chaperone required for the assembly of the F4 fimbriae of enterotoxigenic Escherichia coli. The FaeE crystal structure shows a dimer formed by interaction between the pilin-binding interfaces of the two monomers. Dimerization and tetramerization have been observed previously in crystal structures of fimbrial chaperones and have been suggested to serve as a self-capping mechanism that protects the pilin-interactive surfaces in solution in the absence of the pilins. However, thermodynamic and biochemical data show that FaeE occurs as a stable monomer in solution. Other lines of evidence indicate that self-capping of the pilin-interactive interfaces is not a mechanism that is conservedly applied by all periplasmic chaperones, but is rather a case-specific solution to cap aggregation-prone surfaces. [source] Chaperones of the type III secretion pathway: jacks of all tradesMOLECULAR MICROBIOLOGY, Issue 1 2002Anne-Laure Page Summary The type III secretion (TTS) pathway is used by many Gram-negative bacteria to inject virulence proteins into cells of their host. The activity of the TTS apparatus is controlled by external signals and, in certain conditions, production and secretion are not coupled. Storage of some proteins before secretion involves their association with specific chaperones. Three classes of TTS chaperones have been distinguished according to whether they associate with: (i) one; (ii) several effector proteins; or (iii) the two translocators that allow passage of effectors across the membrane of eukaryotic cells. These chaperones are required for stabilization of their substrate(s) and prevention of their premature interactions with other partners during storage. They also play a role in secretion of their substrate(s). Some chaperones are also involved in transcriptional regulation of certain genes in response to the activity of secretion. The flagellar export apparatus is closely related to the TTS apparatus and some proteins of the flagellar export system have also been proposed to be chaperones that prevent premature interactions between the flagellum subunits. [source] Crystallization and rhenium MAD phasing of the acyl-homoserinelactone synthase EsaIACTA CRYSTALLOGRAPHICA SECTION D, Issue 12 2001William T. Watson Acyl-homoserine- l -lactones (AHLs) are diffusible chemical signals that are required for virulence of many Gram-negative bacteria. AHLs are produced by AHL synthases from two substrates, S -adenosyl- l -methionine and acyl-acyl carrier protein. The AHL synthase EsaI, which is homologous to the AHL synthases from other pathogenic bacterial species, has been crystallized in the primitive tetragonal space group P43, with unit-cell parameters a = b = 66.40, c = 47.33,Å. The structure was solved by multiple-wavelength anomalous diffraction with a novel use of the rhenium anomalous signal. The rhenium-containing structure has been refined to a resolution of 2.5,Å and the perrhenate ion binding sites and liganding residues have been identified. [source] Conserved features of type III secretionCELLULAR MICROBIOLOGY, Issue 9 2004A. P. Tampakaki Summary Type III secretion systems (TTSSs) are essential mediators of the interaction of many Gram-negative bacteria with human, animal or plant hosts. Extensive sequence and functional similarities exist between components of TTSS from bacteria as diverse as animal and plant pathogens. Recent crystal structure determinations of TTSS proteins reveal extensive structural homologies and novel structural motifs and provide a basis on which protein interaction networks start to be drawn within the TTSSs, that are consistent with and help rationalize genetic and biochemical data. Such studies, along with electron microscopy, also established common architectural design and function among the TTSSs of plant and mammalian pathogens, as well as between the TTSS injectisome and the flagellum. Recent comparative genomic analysis, bioinformatic genome mining and genome-wide functional screening have revealed an unsuspected number of newly discovered effectors, especially in plant pathogens and uncovered a wider distribution of TTSS in pathogenic, symbiotic and commensal bacteria. Functional proteomics and analysis further reveals common themes in TTSS effector functions across phylogenetic host and pathogen boundaries. Based on advances in TTSS biology, new diagnostics, crop protection and drug development applications, as well as new cell biology research tools are beginning to emerge. [source] Integration of environmental and host-derived signals with quorum sensing during plant,microbe interactionsCELLULAR MICROBIOLOGY, Issue 3 2004J. A. Newton Summary Many plant-associated microbes use secreted autoinducer molecules, including N -acylhomoserine lactones (AHLs), to regulate diverse behaviours in association with their population density (quorum sensing). Often, these responses are affected by environmental conditions, including the presence of other AHL-producing bacterial species. In addition, plant-derived metabolites, including products that arise as a direct result of the bacterial infection, may profoundly influence AHL-regulated behaviours. These plant products can interact directly and indirectly with the quorum-sensing network and can profoundly affect the quorum-sensing behaviour. Local conditions on a microscopic scale may affect signal molecule longevity, stability and accumulation, and this could be used to give information in addition to cell density. Furthermore, in many Gram-negative bacteria, AHL signalling is subservient to an additional two-component signalling system dependent upon homologues of GacS and GacA. The signal(s) to which GacS responds are not known, but recent research suggests that a self-produced ligand may be being detected. This review will focus on two well-studied examples of AHL-regulated plant-associated behaviour, Erwinia carotovora and Agrobacterium tumefaciens, to illustrate the complexity of such signalling networks. [source] | ||||||||||