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Selected AbstractsIn vivo phosphorylation of regulatory light chain of myosin II in sea urchin eggs and its role in controlling myosin localization and function during cytokinesisCYTOSKELETON, Issue 2 2008Ryota Uehara Abstract Phosphorylation of myosin regulatory light chain (RLC) at Ser19 (mono-phosphorylation) promotes filament assembly and enhances actin-activated ATPase activity of non-muscle myosin, while phosphorylation at both Ser19 and Thr18 (di-phosphorylation) further enhances the ATPase activity. However, it has not well been addressed which type of phosphorylation is important in regulating myosin during cytokinesis. Here, we investigated subcellular localization in sea urchin eggs of mono-phosphorylated and di-phosphorylated RLC by both quantitative biochemical and spatiotemporal cytological approaches. Mono-phosphorylated RLC was dominant in the equatorial cortex throughout the whole process of cytokinesis. Inhibition of myosin light chain kinase (MLCK) decreased mono-phosphorylated RLC both in the cortex and in the cleavage furrow, and blocked both formation and contraction of the contractile ring. Two different types of ROCK inhibitor gave inconsistent results: H1152 blocked both RLC mono-phosphorylation in the cleavage furrow and contraction of the contractile ring, while Y27632 affected neither the mono-phosphorylation nor cell division. These results suggest that there may be other targets of H1152 than ROCK, which is involved in the RLC phosphorylation in the cleavage furrow. Furthermore, it was revealed that localization of myosin heavy chain in the cleavage furrow, but not in the cortex, was perturbed by inhibition of RLC mono-phosphorylation. These results suggested that RLC mono-phosphorylation by more than two RLC kinases play a main role in regulation and localization of myosin in the dividing sea urchin eggs. Cell Motil. Cytoskeleton 2007. © 2007 Wiley-Liss, Inc. [source] Eyes and vision in Arion rufus and Deroceras agreste (Mollusca; Gastropoda; Pulmonata): What role does photoreception play in the orientation of these terrestrial slugs?ACTA ZOOLOGICA, Issue 2 2009Marina V. Zieger Abstract This paper deals with the orientational behaviour in the two terrestrial slugs Arion rufus and Deroceras agreste. It presents anatomical details of their eyes and provides an appraisal of the eyes' optical system. In both species the retinae contain two principal types of cell: photoreceptive and pigmented supportive cells. While only the eye of A. rufus apparently contains neurosecretory neurones, that of D. agreste is the only one equipped with a small additional retina with its own separate lens. Lens shapes vary between ovoid (A. rufus) and spherical (D. agreste). Our results demonstrate that the camera-type eyes in A. rufus and D. agreste have optical systems that do not allow the production of a sharp image on the retina. The slugs demonstrate negative visually mediated phototactic behaviour, but no polarization sensitivity. Only one aspect of the visual environment, namely the overall distribution of light and dark, seems to be important for these slugs. As the main role of the slugs' photoreceptors is to monitor environmental brightness and to assist the animal in orientating towards dark places, we conclude that these slugs do not need to perceive sharp images. [source] The effect of foundation embedment on inelastic response of structuresEARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 4 2009Mojtaba Mahsuli Abstract In this research, a parametric study is carried out on the effect of soil,structure interaction on the ductility and strength demand of buildings with embedded foundation. Both kinematic interaction (KI) and inertial interaction effects are considered. The sub-structure method is used in which the structure is modeled by a simplified single degree of freedom system with idealized bilinear behavior. Besides, the soil sub-structure is considered as a homogeneous half-space and is modeled by a discrete model based on the concept of cone models. The foundation is modeled as a rigid cylinder embedded in the soil with different embedment ratios. The soil,structure system is then analyzed subjected to a suit of 24 selected accelerograms recorded on alluvium deposits. An extensive parametric study is performed for a wide range of the introduced non-dimensional key parameters, which control the problem. It is concluded that foundation embedment may increase the structural demands for slender buildings especially for the case of relatively soft soils. However, the increase in ductility demands may not be significant for shallow foundations with embedment depth to radius of foundation ratios up to one. Comparing the results with and without inclusion of KI reveals that the rocking input motion due to KI plays the main role in this phenomenon. Copyright © 2008 John Wiley & Sons, Ltd. [source] Extracellular excitatory amino acids increase in the paraventricular nucleus of male rats during sexual activity: main role of N -methyl- d -aspartic acid receptors in erectile functionEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 9 2004Maria Rosaria Melis Abstract The concentrations of glutamic and aspartic acids were measured in the dialysate obtained with vertical microdialysis probes implanted into the paraventricular nucleus of the hypothalamus of sexually potent male rats during sexual activity. Animals showed noncontact erections when put in the presence of, and copulated with, a receptive (ovarietomized oestrogen- and progesterone-primed) female rat. The concentrations of glutamic and aspartic acids in the paraventricular dialysate increased by 37 and 80%, respectively, above baseline values during exposure to the receptive female rat and by 55 and 127%, respectively, during copulation. No changes in the concentrations of glutamic and aspartic acids were detected in the paraventricular dialysate when sexually potent male rats were exposed to nonreceptive (ovariectomized not oestrogen- and progesterone-primed) female rats or when impotent male rats were used. The injection into the paraventricular nucleus of the excitatory amino acid receptor antagonist dizocilpine (5 µg), a noncompetitive N -methyl- d -aspartic acid receptor antagonist, reduced noncontact erections and significantly impaired copulatory activity. The ,-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor antagonist 6-cyano-7-nitro-quinoxaline-2,3-dione (5 µg) was also able to impair copulatory activity, but to a much lower extent than dizocilpine. In contrast, (±)-2-amino-4-phosphono-butanoic acid, a metabotropic receptor antagonist (5 µg), was found to be ineffective. These results confirm the involvement of the paraventricular nucleus in the control of erectile function and copulatory behaviour and show that excitatory amino acid concentration increases in the paraventricular nucleus when penile erection occurs in physiological contexts. [source] Corporate Social Responsibility European StyleEUROPEAN LAW JOURNAL, Issue 2 2008Olivier De Schutter This article explains how, while CSR may have been initially an idea about the scope of the responsibility of companies towards their environment, it has now become a process in which the representatives of the business community have come to occupy the main role, and whose purpose is to promote learning among business organisations, rather than to identify the components of a regulatory framework for CSR. The central question now, therefore, is whether the so-called ,business case' for CSR is strong enough, so that we may hope that the forces of market will suffice to encourage companies to behave responsibly, over and above their obligation to comply with their legal obligations. The article shows, however, that this case rests on certain presuppositions about markets and the business environment, which cannot be simply assumed, but should be affirmatively created by a regulatory framework for CSR. Following the introduction, it proceeds in four stages. First, it examines the development of CSR in the EU. Second, it offers a critical examination of the so-called ,business case' for CSR, taking into account the growing diversity within the enlarged EU. It then discusses, as an alternative, what a regulatory framework for CSR could resemble, highlighting a number of initiatives which have been taken in this regard by the EU. The article finally concludes that, since the failure of the European Multi-Stakeholder Forum on CSR in 2004, the debate has made a turn in the wrong direction, both because of the mistaken view that the establishment of a regulatory framework for CSR would threaten the competitiveness of European companies, and because of the naive (and contradictory) view that reliance on market mechanisms will suffice to ensure that corporations will seek to minimise the negative social and environmental impacts of their activities, even in circumstances where they are not legally obliged to do so. [source] TESTING THE ROLE OF INTERSPECIFIC COMPETITION IN THE EVOLUTIONARY ORIGIN OF ELEVATIONAL ZONATION: AN EXAMPLE WITH BUARREMON BRUSH-FINCHES (AVES, EMBERIZIDAE) IN THE NEOTROPICAL MOUNTAINSEVOLUTION, Issue 5 2007Carlos Daniel Cadena Interspecific competition might drive the evolution of ecological niches and result in pairs of formerly competing species segregating along ecological gradients following a process of character displacement. This mechanism has been proposed to account for replacement of related species along gradients of elevation in many areas of the world, but the fundamental issue of whether competition is responsible for the origin of elevational replacements has not been tested. To test hypotheses about the role of interspecific competition in the origin of complementary elevational ranges, I combined molecular phylogenetics, phylogeography, and population genetic analyses on Buarremon torquatus and B. brunneinucha (Aves, Emberizidae), whose patterns of elevational distribution suggest character displacement or ecological release. The hypothesis that elevational distributions in these species changed in opposite directions as a result of competition is untenable because: (1) a historical expansion of the range of B. brunneinucha into areas occupied by B. torquatus was not accompanied by a shift in the elevational range of the former species; (2) when B. brunneinucha colonized the range of B. torquatus, lineages of the latter distributions had already diverged; and (3) historical trends in effective population size do not suggest populations with elevational ranges abutting those of putative competitors have declined as would be expected if competition caused range contractions. However, owing to uncertainty in coalescent estimates of historical population sizes, the hypothesis that some populations of B. torquatus have declined cannot be confidently rejected, which suggests asymmetric character displacement might have occurred. I suggest that the main role of competition in elevational zonation may be to act as a sorting mechanism that allows the coexistence along mountain slopes only of ecologically similar species that differ in elevational distributions prior to attaining sympatry. The contrasting biogeographic histories of B. brunneinucha and B. torquatus illustrate how present-day ecological interactions can have recent origins, and highlights important challenges for testing the hypothesis of character displacement in the absence of data on population history and robust reconstructions of the evolution of traits and geographic ranges. [source] A pathway through interferon-, is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cellsFEBS JOURNAL, Issue 19 2003Motohiro Matsuura Production of nitric oxide (NO) in response to bacterial lipopolysaccharide (LPS) was investigated using cultures of mouse peritoneal exudate cells (PEC) and the macrophage cell line RAW264.7. In the presence of anti-(interferon-,) (IFN-,), NO production was markedly suppressed in the PEC culture but not in the RAW264.7 culture. In the PEC culture, LPS induced both IFN-, production and activation of IFN response factor-1, which leads to the gene expression of inducible NO synthase, but neither was induced in the culture of RAW264.7 cells. In addition to anti-(IFN-,), antibodies against interleukin (IL)-12 and IL-18 showed a suppressive effect on LPS-induced NO production in the PEC culture, and these antibodies in synergy showed strong suppression. Stimulation of the PEC culture with IL-12 or IL-18 induced production of IFN-, and NO, and these cytokines, in combination, exhibited marked synergism. Stimulation of the culture with IFN-, induced production of NO, but not IL-12. The macrophage population in the PEC, prepared as adherent cells, responded well to LPS for IL-12 production, but weakly for production of IFN-, and NO. The macrophages also responded well to IFN-, for NO production. For production of IFN-, by stimulation with LPS or IL-12 + IL-18, nonadherent cells were required in the PEC culture. Considering these results overall, the indirect pathway, through the production of intermediates (such as IFN-,-inducing cytokines and IFN-,) by the cooperation of macrophages with nonadherent cells, was revealed to play the main role in the LPS-induced NO production pathway, as opposed to the direct pathway requiring only a macrophage population. [source] Proteome-guided search for influenza A B-cell epitopesFEMS IMMUNOLOGY & MEDICAL MICROBIOLOGY, Issue 1 2009Guglielmo Lucchese Abstract The influenza A linear peptide epitopes recognized by murine antibodies, and currently cataloged at http://www.immuneepitope.org, were examined for the identity score to the host mouse proteome. It was found that almost all of the linear viral determinants are (or contain) regions formed by pentapeptide fragments with no or only very low similarity to the murine proteins. The present study adds to previous reports in suggesting a main role of amino acid sequence similarity in the modulation and definition of the B-cell epitope repertoire, inspiring innovative vaccine approaches able to avoid cross-reactive autoimmune collateral phenomena, and addressing future research in the study of immunity against the influenza A virus and infectious diseases in general. [source] Cyclin G1 associates with MDM2 and regulates accumulation and degradation of p53 proteinGENES TO CELLS, Issue 8 2002Shinya H. Kimura Background: Cyclin G1 is a transcriptional target of p53 and is induced by DNA damage in a p53 dependent manner. Analysis of cyclin G1 disrupted mice demonstrated that cyclin G1 is involved in many of the functions regulated by p53 such as apoptosis, growth control and check point regulation in response to DNA damage. The results suggest that the main role of cyclin G1 is to mediate or regulate the function of p53. Results: Western blot analysis revealed that the accumulation of p53 protein during the initial 24 h period following DNA damage is reduced in cyclin G1,/, cells compared to wild-type cells. This decrease in p53 accumulation could be recovered by introducing a cDNA expressing cyclin G1. Cyclin G1 interacted directly with MDM2 and promoted the formation of the ARF/MDM2 complex within the initial 24 h period following DNA damage. Furthermore, 48 h after irradiation, accumulation of p53 protein was enhanced in cyclin G1,/, cells compared to wild-type cells. In contrast, in 48 h postirradiated wild-type cells, the cyclin G1-MDM2 complex was found not to be associated with ARF but with the B,, subunit of protein phosphatase A. Conclusion: These results suggest that cyclin G1 stabilizes and promotes the degradation of p53 protein by associating, respectively, with MDM2 complexes containing ARF and PP2A. [source] Bir1/Cut17 moving from chromosome to spindle upon the loss of cohesion is required for condensation, spindle elongation and repairGENES TO CELLS, Issue 9 2001Jun Morishita Background In mammals, proteins containing BIR domains (IAPs and survivin) are implicated in inhibiting apoptosis and sister chromatid separation. In the nematode, Bir1 is required for a proper localization of aurora kinase, which moves from the mitotic chromosome in metaphase to the spindle midzone in anaphase as a passenger. Fission yeast Bir1/Pbh1 is essential for normal mitosis. Results A temperature sensitive mutant cut17-275 exhibits the defect in condensation and spindle elongation at 36 °C, while securin is degraded. Gene cloning shows that the cut17+ gene is identical to bir1+/pbh1+. At 26 °C, cut17-275 is UV sensitive as the repair of DNA damage is severely compromised. Bir1/Cut17 is a nuclear protein in interphase, which is then required for recruiting condensin to the mitotic nucleus, and concentrates to form a discrete number of dots from prometaphase to metaphase. Once the chromatids are separated, Bir1/Cut17 no longer binds to kinetochores and instead moves to the middle of spindle. Chromatin immunoprecipitation suggested that Bir1/Cut17 associates with the outer repetitious centromere region in metaphase. Following the initiation of anaphase the protein switches from being a chromosomal protein to a spindle protein. This transit is stringently regulated by the state of sister chromatid cohesion proteins Mis4 and Rad21. Ark1, is an aurora kinase homologue whose mitotic distribution is identical to, and under the control of Bir1/Cut17. Conclusions Bir1/Cut17 and Ark1 act as ,passengers' but they may play a main role as a recruitment factor, essential for condensation, spindle elongation and DNA repair. Bir1/Cut17 should have roles both in mitotic and in interphase chromosome. The proper location of Ark1 requires Bir1/Cut17, and the mitotic localization of Bir1/Cut17 requires sister cohesion. [source] HORMONAL CONTROL OF THE VITELLOGENESIS IN THE JAPANESE OAK SILKWORM, ANTHERAEA YAMAMAZ (LEPIDOPTERA: SATURNIIDAE)INSECT SCIENCE, Issue 1 2002YE Gong-yin Abstract Effects of ecdysteroid and juvenile hormone (JH) on vitellogenesis of the Japanese oak silkworm, Antheraea yamami are reported in this article. After topical treatment with 20-hydroxyecdysone alone or JH analog (i.e. methoprene) alone and combined treatment with these two chemicals, vitellogenin (Vg) titers in the fat body and haemolymph at the pupal stage were mostly higher than those of the control, indicating that both ecdysteroid and JH exerted a promoting effect on the synthesis of Vg. In contrast, the Vg uptake was markedly inhibited by JH while stimulating effect of the ecdysteroid could be shown that vitellin (Vt) titer in the ovary was lower after methoprene treatments, but higher after 20-hydroxyecdyson treatments. Meanwhile, effects of these two hormones on Vg synthesis in the fat body were also tested with the incubation in vitro with Grace medium containing H-leucine and the hormones. The results demonstrated that Vg synthesis was stimulated after treating with methoprene alone or 20-hydroxyecdysone alone and combined treating with these two chemicals, and particularly ecdysteroid had more marked positive effect. To comprehensively concluded our results, it could be regarded that ecdysteroid play the main role in the regulation of vitellogenesis for the Japanese oak silkworm. [source] Redox Reactions and Electron Transfer Across the Red Cell MembraneIUBMB LIFE, Issue 7 2003Eleanor Kennett Abstract Plasma membrane electron transport systems appear to be ubiquitous. These systems are implicated in hormone signal transduction, cell growth and differentiation events as well as protection from oxidative stress. The red blood cell is constantly exposed to oxidative stress; protection against the reactive species generated during this process may be the main role of its membrane electron transport systems. Membrane redox activity has been studied for over three-quarters of a century, and yet many questions remain regarding its identity and likely roles: are electron transfers by distinct and specific mechanisms; what are the physiological donors and acceptors; and how do these systems affect metabolism? Current evidence suggests that the human erythrocyte membrane contains a number of distinct electron transfer systems, some of which, at least, involve membrane proteins, and NADH or ascorbate as electron donors. The activity of these systems appears to be closely related to the metabolic state of the cell, suggesting that mediation of reducing equivalents across the plasma membrane allows redox buffering of each environment, intra- and extracellular, by the other. We have decided to study this from a new perspective, NMR spectroscopy the area of our own technical expertise, hence this review is slanted towards this more recent analysis. IUBMB Life, 55: 375-385, 2003 [source] Catalytic performance of Brønsted acid sites during esterification of acetic acid with ethyl alcohol over phosphotungestic acid supported on silicaJOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY, Issue 6 2007Abd El-Aziz A Said Abstract Different ratios of phosphotungestic acid supported on silica gel were prepared by an impregnation method with PWA loadings ranging from 1 to 30% w/w and calcined at 350 and 500 °C for 4 h in a static air atmosphere. The catalysts were characterized by thermogravimety (TG), differential thermal analysis (DTA), X-ray diffraction, FT-IR spectroscopy and N2 adsorption measurements. The surface acidity and basicity of the catalyst were investigated by the dehydration,dehydrogenation of isopropanol and the adsorption of pyridine (PY) and 2,6-dimethyl pyridine (DMPY). The gas-phase estrification of acetic acid with ethanol was carried out at 185 °C in a conventional fixed-bed reactor at 1 atm using air as carrier gas. The results clearly revealed that the catalyst containing 10% w/w PWA/SiO2 is the most active and delivers reaction selectively to ester with 85% yield. The Brønsted acid site resulting from hydroxylation of tungsten oxide plays the main role in the formation of ester. Copyright © 2007 Society of Chemical Industry [source] Roles of cations, electronegativity difference, and anionic interlayer interactions in the metallic versus nonmetallic character of Zintl phases related to arsenicJOURNAL OF COMPUTATIONAL CHEMISTRY, Issue 13 2008Pere Alemany Abstract A first-principles Density Functional Theory study of several layered solids structurally related to rhombohedral arsenic has been carried out. The electronic structures of rhombohedral arsenic, CaSi2, CaAl2Si2, KSnSb, and SrSn2As2 are discussed in detail, emphasizing on the origins of their metallic or nonmetallic behaviours. It is found that all of these systems are metallic except KSnSb. Electronegativity differences between the elements in the anionic sublattice and/or direct interlayer interactions play the main role in controlling the conductivity behavior. CaSi2 exhibits a peculiar feature since the cation directly influences the conductivity but is not essential for its appearance. Cation-anion interactions are shown to have an important covalent contribution, but despite this fact and the metallic character found for most of these phases, the Zintl approach still provides a valid approximation to their electronic structure. © 2008 Wiley Periodicals, Inc. J Comput Chem 2008 [source] Expression of CD73/ecto-5,-nucleotidase on human gingival fibroblasts and contribution to the inhibition of interleukin-1,-induced granulocyte-macrophage colony stimulating factor productionJOURNAL OF PERIODONTAL RESEARCH, Issue 1 2004Eiji Nemoto Background and objectives:, CD73/5,-nucleotidase (5,-NT) is an ectoenzyme that participates in immune/inflammatory reactions. We examined the possible expression of CD73/5,-NT on human gingival fibroblasts (hGF), which are important to the immune/inflammatory system in periodontal tissue. Methods and results:, We demonstrated that CD73/5,-NT was expressed on hGF by flow cytometry. We found that pre-treatment of hGF with 5,-AMP induced marked inhibition of granulocyte-macrophage colony-stimulating factor (GM-CSF) production from hGF upon stimulation with interleukin-1, (IL-1,) by enzyme-linked immunosorbent assay (ELISA). A specific inhibitor of 5,-NT, adenosine 5,-[,,,-methylene] diphosphate blocked the inhibition of GM-CSF production, suggesting that adenosine converted from 5,-AMP acts on the inhibitory effects. The GM-CSF inhibition suggested that A3 receptor might be involved. The rank order of agonists was found to be (N6 -benzyl-5,- N -ethylcarboxamidoadenosine) A3 receptor agonist , (2-chloroadenosine) non-selective agonist > (CGS-21680) A2A receptor agonist > adenosine , (N6 -cyclohexyladenosine) A1 agonist. Further support for the main role of A3 receptor was the binding A3 antagonist [9-chloro-2-(2-furanyl)-5-([phenylacetyl]amino)[1,2,4]-triazolo[1,5- c]quinazdine] reversed the effect of adenosine, but no significant reverse was observed by A1 (1,3-dipropyl-8-cyclopentylxanthine), A2 [3,7-dimethyl-1-(2-propargyl)xanthine], A2A[8-(3-chlorostyryl)caffeine], and A2B (alloxazine) antagonists. The CD73/5,-NT expression was increased upon stimulation with gamma-interferon, but not other stimulants such as tumor necrosis factor-alpha, IL-4, lipopolysaccharide from Porphyromonas gingivalis and Escherichia coli, and fimbriae from P. gingivalis, and this increase was correlated with the enhanced GM-CSF inhibition by 5,-AMP but not adenosine. Conclusions:, These findings suggested that CD73/5,-NT on hGF exerts an anti-inflammatory effects in periodontal disease by conversion from 5,-AMP to adenosine. [source] Annealing effect on an exchange-biasing Co/IrMn systemPHYSICA STATUS SOLIDI (C) - CURRENT TOPICS IN SOLID STATE PHYSICS, Issue 12 2007Yuan-Tsung Chen Abstract The top-configuration Co(y)/IrMn(90 Å) exchange-biasing phenomenon has been studied by sputtering method with two conditions: (a) the substrate temperature (Ts) was kept at room temperature (RT) only, and (b) Ts = RT with an in-plane field (h) = 500 Oe deposition and postdeposition annealing in the field at TA = 250 °C for 1 h, with the samples field cooled to RT. High resolution electron cross-sectional transmission electron microscopy (HR X-TEM) reveals that the IrMn (111) texturing plays a main role to exchange-biasing field (Hex) and interfacial energy (Jk). The Hex versus y result shows that Hex increases when y decreases in case (b). Since Jk = HexMsy, where Ms is Co magnetization, it is easy to derive Hex = Jk/(Msy). Therefore, if Hex is inversely proportional to y, with Jk/Ms constant, we find Hexy = constant. In case (a), Hex is very small in general, while in case (b), Hex is of the order of 60-180 Oe. Moreover, the y dependence of Jk is similar to that of Ms for each curve. Finally, the Hc is inversely proportional to y because of the surface pinning effects from the Ta/Co and Co/IrMn interfaces. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Relationship between seed and clonal growth in the reproduction of Carex rugulosa Kük. in riverside meadowsPLANT SPECIES BIOLOGY, Issue 2 2008SATORU ARAKI Abstract Carex rugulosa Kük. develops riverside vegetation in brackish marshes along the lower parts of rivers. Because the rhizomatous ramets grow densely (590,950/m2) in its developed population, it is expected that seedlings cannot get enough light to grow if seeds germinate in crowded ramets. We studied the reproductive contribution of seed in a marshy meadow of the Ohashi River, Japan. The number of seeds produced was 6900,14 200/m2 in 2002. The number of buried seeds in the following germination season differed among sites from 1190 to 2690/m2. No seedlings were observed at plots where the ramets from rhizomes grew densely. In contrast, seedlings appeared in plots where all rhizomatous ramets were artificially cut. The number of seedlings corresponded to 17.5,39.5% of that of buried seeds. All these seedlings were submerged and died when it rained heavily. In the genotype analysis using allozyme detection, one of the phosphoglucoisomerase loci (Pgi-2) indicated a pair of alleles. Among eight plots (each plot was 50 or 60-cm square), four showed an excess of heterozygotes and two showed homozygote domination in the Pgi-2 genotype. These results suggest that: (i) seed germination is suppressed in crowded ramets; (ii) seedling survival is severely reduced by inundation; and (iii) the population is usually maintained by clonal growth. As germination is induced in open areas, it is hypothesized that the main role of seed is recovery after vegetation decline caused by environmental stresses or colonization to other areas without dense vegetation. [source] Influence of the rubbery phase on the crystallinity and thermomechanical properties of poly(3-hydroxybutyrate)/elastomer blendsPOLYMER INTERNATIONAL, Issue 6 2010Patrícia S Calvão Abstract Poly(3-hydroxybutyrate) (PHB) is a very promising biopolymer. In order to improve its processability and decrease its brittleness, PHB/elastomer blends can be prepared. In the work reported, the effect of the addition of a rubbery phase, i.e. ethylene,propylene,diene terpolymer (EPDM) or poly(vinyl butyral) (PVB), on the properties of PHB was studied. The effects of rubber type and of changing the PHB/elastomer blend processing method on the crystallinity and physical properties of the blends were also investigated. For blends based on PHB, the main role of EPDM is its nucleating effect evidenced by a decrease of crystallization temperature and an increase of crystallinity with increasing EPDM content regardless of the processing route. While EPDM has a weak effect on PHB glass transition temperature, PVB induces a marked decrease of this temperature thank to its plasticizer that swells the PHB amorphous phase. A promising solution to improve the mechanical properties of PHB seems to be the melt-processing of PHB with both plasticizer and EPDM. In fact, the plasticizer is more efficient than the elastomer in decreasing the PHB glass transition temperature and, because of the nucleating effect of EPDM, the decrease of the PHB modulus due to the plasticizer can be counterbalanced. Copyright © 2010 Society of Chemical Industry [source] Sperm chromatin integrity in young men with no experiences of infertility and men from idiopathic infertility couplesANDROLOGIA, Issue 3 2009R. Rybar Summary Damage to the genetic component of spermatozoa seems to play the main role in a majority of cases where current approaches fail to reveal the specific cause of male infertility. In this study, we compared semen quality in men assigned to two defined groups: men from couples with unexplained infertility , idiopathic infertility (A) and young men with no experiences of infertility (B). All samples were examined by standard ejaculate analysis and sperm chromatin structure assay (SCSA). Sperm chromatin damage was significantly higher in men from group A than in those from group B. Similar results were obtained by comparison of men from group A (all men were normozoospermic) with normozoospermic men from group B. According to these results, we can suppose that chromatin disorders may be the causal factor of subfertility or infertility in some of these men. No evidence for a strong association between chromatin disorders and standard parameters of ejaculates was found. We failed to confirm a relationship between smoking and sperm quality in men from any of the investigated groups. SCSA is a method that facilitates the identification of infertile men who otherwise show normal semen variables. [source] Scent-Marking of Giant Otter in the Southern Pantanal, BrazilETHOLOGY, Issue 3 2009Caroline Leuchtenberger Giant otters live in social groups, consisting of a mating pair and one or two litters. Groups are territorial and mark their territories often with scent-marks. Our objectives were to evaluate the frequencies of marking and over-marking according to the social status of the individuals and to define the different postures used during the marking. We observed four groups, totaling 25 individuals (five alpha males, four alpha females, seven adult females, one adult male and eight juveniles) with group size ranging between four and 13 individuals. The study was conducted between July 2006 and July 2007 in the Vermelho River and in a stretch of the Miranda River, in the Southern Pantanal. We observed the groups for a total of 2006 min and recorded 95 events of marking totaling 84.9 min. Time spent marking varied between groups and ranged from 4.3 to 44.7 min. The alpha males marked more frequently (62% of marking events, 55 min) than the alpha females (17% of marking events, 13.6 min). Of the 59 events of scent-marking by the alpha males, 32 over-marked the marks of other individuals from the group. Of the 16 events of scent-marking of the alpha females, five over-marked that of other females from the same group. When scent-marking, alpha males used the ,stepping' posture most frequently (63%), then ,fore-paw rubbing' (24%), ,latrine use' (7%), and ,body rubbing' (6%). Alpha females used the ,stepping' posture most frequently (65%), then ,latrine use' (19%) and ,fore-paw rubbing' (12%), with only one event of ,body rubbing' observed during marking. Subordinate females used the ,stepping' posture (76%) and ,latrine use' (24%) during marking. Scent-marking can play many roles in mammals and for giant otters, and the main roles appear to be communication of social and sexual status and territorial defense. [source] Nitric Oxide, Mitochondria, and Cell DeathIUBMB LIFE, Issue 3-5 2001Guy C. Brown Abstract NO or its derivatives (reactive nitrogen species: RNS) have three types of actions on mitochondria: 1) reversible inhibition of mitochondrial respiration at cytochrome oxidase by NO, and irreversible inhibition at multiple sites by RNS; 2) stimulation of mitochondrial production of superoxide, hydrogen peroxide, and peroxynitrite by NO; and 3) induction of mitochondrial permeability transition (MPT) by RNS. Similarly there are three main roles of mitochondria in NO-induced cell death: a) NO inhibition of respiration can induce necrosis (or excitotoxicity in neurons) and inhibit apoptosis if glycolysis is insufficient to compensate, b) RNS- or oxidant-induced signal transduction or DNA damage may activate the mitochondrial pathway to apoptosis, and c) RNS-induced MPT may induce apoptosis or necrosis. [source] Border crossing of Muslim women in southern-border provinces of ThailandASIA PACIFIC VIEWPOINT, Issue 1 2009Nisakorn Klanarong Abstract In general, the main roles of married Thai Muslim women were as home makers good wives and good mothers. Nevertheless, both married and single women from rural areas have been increasingly obliged to work outside the household, locally and in other countries. People in rural areas are now faced with the difficulty of maintaining their livelihoods if they depended on agricultural production alone. In some instances, female migration might be a response to families not being able to survive on the incomes earned by the male household heads. In response, women in southern Thailand provinces use long-standing social networks that facilitate their migration for work, because they benefit from the close proximity, language, and religion that they share with the destination area. Commonly, they travel to work in Malaysia by using a border pass, while some travel and work without any documents. The effects of crossing national borders on migrants themselves and on their communities are mixed, generally positive from an economic perspective, but negative from a social viewpoint. Socially negative responses reflect a system of social control in the region based on patriarchy. [source] Supraspinal modulation of pain by cannabinoids: the role of GABA and glutamateBRITISH JOURNAL OF PHARMACOLOGY, Issue 5 2007K Rea Recent physiological, pharmacological and anatomical studies provide evidence that one of the main roles of the endocannabinoid system in the brain is the regulation of ,-aminobutyric acid (GABA) and glutamate release. This article aims to review this evidence in the context of its implications for pain. We first provide a brief overview of supraspinal regulation of nociception, followed by a review of the evidence that the brain's endocannabinoid system modulates nociception. We look in detail at regulation of supraspinal GABAergic and glutamatergic neurons by the endocannabinoid system and by exogenously administered cannabinoids. Finally, we review the evidence that cannabinoid-mediated modulation of pain involves modulation of GABAergic and glutamatergic neurotransmission in key brain regions. British Journal of Pharmacology (2007) 152, 633,648; doi:10.1038/sj.bjp.0707440; published online 10 September 2007 [source] | |||||||||||||||||||