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Maximal Temperature (maximal + temperature)

Distribution by Scientific Domains

Medical Sciences	50%

Selected Abstracts

Ultrastructural preservation of rat embryonic dental tissues after rapid fixation and dehydration under microwave irradiation

EUROPEAN JOURNAL OF ORAL SCIENCES, Issue 1 2000
Luciana F. Massa
Adequate preservation of the cells and matrix of mineralising tissues remains difficult, as organic components and initial mineral deposits may be lost during conventional processing for electron microscopy. In this study, we have reduced significantly the processing time using microwave irradiation. Rat molar tooth germs were fixed in 4% glutaraldehyde+4% formaldehyde with 0.1 M sodium cacodylate in a laboratory microwave oven for two periods of 20 s with a maximal temperature of 37°C. After conventional washing and post-fixation, specimens were dehydrated in graded ethanols under microwave irradiation for a total of 7 min 20 s. For comparison, some specimens were processed by conventional methods. After embedding, ultrathin sections were examined by electron microscopy. In differentiating ameloblasts and odontoblasts, plasma membranes, mitochondria, rough endoplasmic reticulum, the Golgi complex, together with all other cytoplasmic organelles exhibited excellent preservation. Microtubules, microfilaments and coated vesicles were particularly evident. Crystal-like mineral deposits were conspicuously present in relation to dentine matrix vesicles and collagen fibrils as well as in enamel matrix. The matrix of forming enamel had a globular electron-lucent appearance. It is concluded that this is a rapid method which provides a preserved or even improved morphology. [source]

Prevalence of Fever in Patients Undergoing Left Atrial Ablation of Atrial Fibrillation Guided by Barium Esophagraphy

JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY, Issue 8 2009
RUBINDER S. RUBY M.D.
Background: Real-time esophageal imaging is critical in avoiding esophageal injury. However, the safety of esophageal imaging with barium has not been specifically explored. Methods: Three hundred seventy consecutive patients underwent left atrial (LA) ablation of atrial fibrillation (AF) under conscious sedation. One hundred eighty-five patients (50%) underwent the ablation procedure with, and 185 patients (50%) underwent the procedure without administration of barium. Fever, as a surrogate for aspiration, was defined as a maximal temperature ,100°F within the first 24 hours following the ablation procedure. Results: Thirty of the 370 patients (8%) developed fever within 24 hours after LA ablation. The prevalence of fever was 9% (17/185) among patients who received barium and 7% (13/185) among those who did not receive barium (P = 0.6). Evaluation revealed the following causes of fever in 14 of the 30 patients (47%) with no difference in prevalence between the 2 groups: pericarditis, venous thromboembolism, hematoma, and infiltrate on chest radiography. Multivariate analysis failed to reveal any factors associated with development of fever. None of the patients experienced serious complications such as respiratory failure or atrioesophageal fistula. Conclusions: Fever may occur in approximately 10% of patients undergoing LA ablation of AF. Administration of barium is not associated with fever or other complications such as aspiration pneumonia. Real-time imaging of the esophagus with barium administration in conjunction with conscious sedation appears to be safe. [source]

Thermophilic (55,65 °C) and Extreme Thermophilic (70,80 °C) Sulfate Reduction in Methanol and Formate-Fed UASB Reactors

BIOTECHNOLOGY PROGRESS, Issue 5 2004
Marcus V. G. Vallero
The feasibility of thermophilic (55,65 °C) and extreme thermophilic (70,80 °C) sulfate-reducing processes was investigated in three lab-scale upflow anaerobic sludge bed (UASB) reactors fed with either methanol or formate as the sole substrates and inoculated with mesophilic granular sludge previously not exposed to high temperatures. Full methanol and formate degradation at temperatures up to, respectively, 70 and 75 °C, were achieved when operating UASB reactors fed with sulfate rich (COD/SO42 - = 0.5) synthetic wastewater. Methane-producing archaea (MPA) outcompeted sulfate-reducing bacteria (SRB) in the formate-fed UASB reactor at all temperatures tested (65,75 °C). In contrast, SRB outcompeted MPA in methanol-fed UASB reactors at temperatures equal to or exceeding 65 °C, whereas strong competition between SRB and MPA was observed in these reactors at 55 °C. A short-term (5 days) temperature increase from 55 to 65 °C was an effective strategy to suppress methanogenesis in methanol-fed sulfidogenic UASB reactors operated at 55 °C. Methanol was found to be a suitable electron donor for sulfate-reducing processes at a maximal temperature of 70 °C, with sulfide as the sole mineralization product of methanol degradation at that temperature. [source]

Computer modeling of optical properties of gold ellipsoidal nanoparticles at laser radiation wavelengths

LASER PHYSICS LETTERS, Issue 2 2005
V. K. Pustovalov
Abstract Processes of laser interaction with metal (gold) nanoparticles are of great interest for laser applications in nanotechnology, engineering, and medicine. Optical properties of nanoparticles determine photophysical and photochemical processes during laser treatment. Computer calculation of efficiency factors of absorption, scattering and extinction of radiation by ellipsoidal gold nanoparticles (two-dimensional ellipsoids of revolution) with small semiaxes in the range 5,100 nm, for some values of aspect ratio and angle of orientation of ellipsoid with respect to direction of laser radiation propagation and for some laser wavelengths is performed. The estimation of absorbed and scattered laser radiation energy by gold ellipsoidal nanoparticles, their maximal temperatures and comparison with experimental data is made. (© 2005 by Astro, Ltd. Published exclusively by WILEY-VCH Verlag GmbH & Co. KGaA) [source]