Home About us Contact
|
Home About us Contact | ||
|
|
||
Malt Extract (malt + extract)
Selected AbstractsResponse surface methodology to optimize the nutritional parameters for enhanced production of jasmonic acid by Lasiodiplodia theobromaeJOURNAL OF APPLIED MICROBIOLOGY, Issue 3 2008P.C. Dhandhukia Abstract Aims:, To find out the cumulative effect of the nutritional parameters and to enhance the production of jasmonic acid (JA) in static fermentation by Lasiodiplodia theobromae using response surface methodology (RSM). Method and Results:, Malt extract, sucrose, NaNO3 and MgSO4.7H2O were analysed by a 30-trial central composite design using RSM for optimizing their concentrations in the medium and the effect of their mutual interaction on JA production. Sucrose and NaNO3 were found highly significant in influencing the JA production. Malt extract and MgSO4.7H2O showed an effect on the JA production in interaction with other variables. When the optimum values of the parameters obtained through RSM (19·95 g l,1 malt extract, 50 g l,1 sucrose, 7·5 g l,1 NaNO3 and 3·51 g l,1 MgSO4.7H2O) were applied, 32% increase in JA production (299 mg l,1) was observed in comparison with 225 mg l,1 of JA produced with same media components not analysed by RSM and subsequently validated the statistical model. Conclusions:, Increase in JA production was achieved by optimizing the nutritional parameters. Significance and Impact of the Study:, This is the first report of using RSM for optimizing a medium for JA production. It resulted in an increase in JA production without augmentation of costly additives. [source] FLOW PROPERTIES OF MALT CONCENTRATE-SWEETENED CONDENSED MILK MIXTURESJOURNAL OF FOOD PROCESS ENGINEERING, Issue 3 2000S. TEJINDER ABSTRACT The rheological characteristics of various malt extract concentrate-sweetened condensed milk mixtures in the ratios of 50:50, 60:40, 70:30 and 80:20 (w/w) were studied at 30 to 80C, using a coaxial viscometer. The magnitudes of flow behavior index varied from 0.42,0.93. Experimental temperatures and concentrations of sweetened condensed milk considerably affected pseudoplasticity, consistency index and yield stress values of the mixtures. At 60 to 80C, the 60:40, 70:30 and 80:20 mixtures showed spectacularly higher flow behavior index values than those of the 50:50. The yield stress values increased with increasing temperature. The increases were considerably high in the 50:50 mixtures. The hulless barley malt concentrate-sweetened condensed milk mixtures, however, were mildly non-Newtonian and showed decreases in the values for yield stress with increasing temperatures. The activation energy values for flow behavior index and yield stress were negative in magnitudes, whereas those of consistency index were positive, which ranged from 1.6 to 15.0 Kcal/g mole. [source] Large-scale submerged fermentation of Antrodia cinnamomea for anti-hepatoma activityJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 13 2008Jih-Hung Pan Abstract BACKGROUND: Submerged cultivation of Antrodia cinnamomea was carried out for manufacturing the fermentation product with anti-hepatoma activity. The fermentation process was optimized for different parameters at shake flask level to obtain products with high inhibition potency against Hep G2 hepatoma cells. Scale-up of the fermentation process was then achieved from 250 mL shake flask to 5 L, 500 L and 5-ton fermenters. RESULTS: Glucose and malt extract were found to be the best carbon and nitrogen sources, respectively. The initial pH of 5.0 and an operating temperature of 22 °C were the best for a product with lowest IC50 value. A shorter cultivation time was required when the scale of fermentation increased from 5 L to 5 tons. The reducing sugar and solids contents of the broth filtrate were correlated exponentially with the IC50 of the ethanolic extract of mycelium for hepatoma cells, and the level of ergosterol in the mycelium extract follows the same profile as the increase in Hep G2 cells inhibition. CONCLUSION: When Antrodia cinnamomea is cultured in a 5-ton fermenter, 4 weeks are required for the fermentation product to reach the highest anti-hepatoma activity. The solid and reducing sugar contents of the broth filtrate as well as the ergosterol content in the ethanol extract of mycelium can serve as the marker during fermentation for manufacturing product with anti-hepatoma activity. Copyright © 2008 Society of Chemical Industry [source] | ||||||||||